TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Demirci, Taylan
A1  - Trzewik, Jürgen
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension of endothelial cells: An LPS based in-vitro sepsis model
JF  - IUBMB Life. 61 (2009), H. 3
Y1  - 2009
SN  - 1521-6543
N1  - Abstracts: Turkish Society of Molecular Medicine, Third International Congress of Molecular Medicine, May 5-8, 2009, Istanbul, Turkey
SP  - 307
EP  - 308
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Wehlitz, V.
A1  - Kayser, Peter
A1  - Figiel-Lange, A.
A1  - Bassam, R.
A1  - Rundstedt, F. von
T1  - Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids
JF  - Plant Pathology
N2  - Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach.
Y1  - 2018
U6  - http://dx.doi.org/10.1111/ppa.12750
SN  - 1365-3059
VL  - 67
IS  - 2
SP  - 358
EP  - 365
PB  - Wiley
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Alexyuk, Madina
A1  - Bogoyavlenskiy, Andrey
A1  - Alexyuk, Pavel
A1  - Moldakhanov, Yergali
A1  - Berezin, Vladimir
A1  - Digel, Ilya
T1  - Epipelagic microbiome of the Small Aral Sea: Metagenomic structure and ecological diversity
JF  - MicrobiologyOpen
N2  - Microbial diversity studies regarding the aquatic communities that experienced or are experiencing environmental problems are essential for the comprehension of the remediation dynamics. In this pilot study, we present data on the phylogenetic and ecological structure of microorganisms from epipelagic water samples collected in the Small Aral Sea (SAS). The raw data were generated by massive parallel sequencing using the shotgun approach. As expected, most of the identified DNA sequences belonged to Terrabacteria and Actinobacteria (40% and 37% of the total reads, respectively). The occurrence of Deinococcus-Thermus, Armatimonadetes, Chloroflexi in the epipelagic SAS waters was less anticipated. Surprising was also the detection of sequences, which are characteristic for strict anaerobes—Ignavibacteria, hydrogen-oxidizing bacteria, and archaeal methanogenic species. We suppose that the observed very broad range of phylogenetic and ecological features displayed by the SAS reads demonstrates a more intensive mixing of water masses originating from diverse ecological niches of the Aral-Syr Darya River basin than presumed before.
KW  - ecological structure
KW  - metagenomics
KW  - microbial diversity
KW  - shotgun sequencing
KW  - Small Aral Sea
Y1  - 2021
U6  - http://dx.doi.org/10.1002/mbo3.1142
SN  - 2045-8827
VL  - 10
IS  - 1
SP  - 1
EP  - 10
PB  - Wiley
CY  - Weinheim
ER  -