TY  - JOUR
A1  - Seifarth, Volker
A1  - Schehl, D.
A1  - Linder, Peter
A1  - Gossmann, Matthias
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Porst, Dariusz
A1  - Preiß, C.
A1  - Kayser, Peter
A1  - Pack, O.
A1  - Temiz Artmann, Aysegül
T1  - Ureplace: development of a bioreactor for in vitro culturing of cell seeded tubular vessels on collagen scaffolds
N2  - The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 %. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days.
KW  - Tissue Engineering
KW  - Bioreaktor
KW  - Organkultur
KW  - Harnleiter
Y1  - 2011
ER  - 
TY  - CHAP
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Demirci, Taylan
A1  - Gierkowski, Jessica R.
A1  - Digel, Ilya
A1  - Gossmann, Matthias
A1  - Temiz Artmann, Aysegül
T1  - rhAPC reduces the endothelial cell permeability via a decrease of cellular mechanical contractile tensions : [abstract]
N2  - In this study, the CellDrum technology quanitfying cellular mechanical tension on a pico-scale was used to investigate the effect of LPS (lipopolysaccharide) on HAoEC (Human Aortic Endothelial Cell) tension.
KW  - Endothelzelle
KW  - Sepsis
KW  - kontraktile Spannung
KW  - rhAPC
KW  - contractile tension
KW  - rhAPC
KW  - celldrum technology
Y1  - 2010
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Demirci, Taylan
A1  - Trzewik, Jürgen
A1  - Linder, Peter
A1  - Temiz Artmann, Aysegül
T1  - Fibroblast response to mechanical stress: role of the adhesion substrate : [abstract]
N2  - Mechanical stimulation of the cells resulted in evident changes in the cell morphology, protein composition and gene expression. Microscopically, additional formation of stress fibers accompanied by cell re-arrangements in a monolayer was observed. Also, significant activation of p53 gene was revealed as compared to control. Interestingly, the use of CellTech membrane coating induced cell death after mechanical stress had been applied. Such an effect was not detected when fibronectin had been used as an adhesion substrate.
KW  - Fibroblast
KW  - Mechanische Beanspruchung
KW  - celldrum technology
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Bassam, Rasha
A1  - Artmann, Gerhard
A1  - Hescheler, Jürgen
A1  - Graef, T.
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Arinkin, Vladimir
A1  - Gossmann, Matthias
A1  - Digel, Ilya
T1  - Alterations in human hemoglobin structure related to red blood cell storage
N2  - The importance of the availability of stored blood or blood cells, respectively, for urgent transfusion cannot be overestimated. Nowadays, blood storage becomes even more important since blood products are used for epidemiological studies, bio-technical research or banked for transfusion purposes. Thus blood samples must not only be processed, stored, and shipped to preserve their efficacy and safety, but also all parameters of storage must be recorded and reported for Quality Assurance. Therefore, blood banks and clinical research facilities are seeking more accurate, automated means for blood storage and blood processing.
KW  - Hämoglobin
KW  - Hämoglobinstruktur
KW  - Blutzellenlagerung
KW  - Hemoglobin structure
KW  - Red blood cell storage
Y1  - 2011
ER  -