TY  - JOUR
A1  - Oosterhuis, Koen
A1  - Öhlschläger, Peter
A1  - Berg, Joost H. van den
A1  - Toebes, Mireille
A1  - Gomez, Raquel
A1  - Schumacher, Ton N.
A1  - Haanen, John B.
T1  - Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7
JF  - International Journal of Cancer
Y1  - 2011
SN  - 1097-0215
VL  - 129
IS  - 2
SP  - 397
EP  - 406
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Henken, F. E.
A1  - Oosterhuis, K.
A1  - Öhlschläger, Peter
A1  - Bosch, L.
A1  - Hooijberg, E.
A1  - Haanen, J. B. A. G.
A1  - Steenbergen, R. D. M.
T1  - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7
JF  - Vaccine
N2  - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.
Y1  - 2012
U6  - https://doi.org/10.1016/j.vaccine.2012.04.013
SN  - 0264-410X
VL  - 30
IS  - 28
SP  - 4259
EP  - 4266
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schelthoff, Christof
A1  - Basermann, Achim
A1  - Reichel, Björn
T1  - Preconditioned CG methods for sparse matrices on massively parallel machines / Basermann, A. ; Reichel, B. ; Schelthoff, C.
JF  - Parallel Computing. 23 (1997), H. 3
Y1  - 1997
SN  - 0167-8191
SP  - 381
EP  - 398
ER  - 
TY  - JOUR
A1  - Engelmann, Ulrich M.
A1  - Shasha, Carolyn
A1  - Teeman, Eric
A1  - Slabu, Iona
A1  - Krishnan, Kannan M.
T1  - Predicting size-dependent heating efficiency of magnetic nanoparticles from experiment and stochastic Néel-Brown Langevin simulation
JF  - Journal of Magnetism and Magnetic Materials
Y1  - 2019
U6  - https://doi.org/10.1016/j.jmmm.2018.09.041
SN  - 0304-8853
VL  - 471
IS  - 1
SP  - 450
EP  - 456
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Anik, Sabri
A1  - Frohberg, Martin G.
A1  - Kapoor, Madan Lal
T1  - Prediction of thermodynamic properties of oxygen in binary metallic solvents
JF  - Zeitschrift für Metallkunde
Y1  - 1983
SN  - 0044-3093
VL  - 74
IS  - 6
SP  - 372
EP  - 375
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Doll, Theodor
A1  - Wagner, Patrick
T1  - Preface / Doll, Theodor ; Wagner, Patrick ; Schöning, Michael J.
JF  - physica status solidi (a) . 206 (2009), H. 3
Y1  - 2009
SN  - 1862-6319
N1  - Special Issue: Engineering of Functional Interfaces (EnFI 08)
SP  - 389
EP  - 390
ER  - 
TY  - JOUR
A1  - Kezerashvili, Roman Ya
A1  - Dachwald, Bernd
T1  - Preface: Solar sailing: Concepts, technology, and missions II
JF  - Advances in Space Research
Y1  - 2021
U6  - https://doi.org/10.1016/j.asr.2021.01.037
SN  - 0273-1177
VL  - 67
IS  - 9
SP  - 2559
EP  - 2560
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Biener, Ernst
A1  - Sasse, T.
A1  - Bökers, H.
T1  - Preliminary or final capping of sanitary landfills? : Technical and economical aspects
JF  - Sardinia 99 : proceedings / Seventh International Waste Management and Landfill Symposium, [4 - 8 October 1999, S. Margherita di Pula, Cagliari, Sardinia, Italy]. Ed.: Thomas H. Christensen. -  Bd. 3 : Barriers, waste mechanics and landfill design
Y1  - 1999
N1  - International Waste Management and Landfill Symposium <7, 1999, Cagliari>
SP  - 287
EP  - 294
PB  - CISA, Environmental Sanitary Engineering Centre
CY  - Cagliari
ER  - 
TY  - JOUR
A1  - Khodaverdi, M.
A1  - Pauly, F.
A1  - Schroder, G.
A1  - Ziemons, Karl
A1  - Sievering, R.
A1  - Halling, H.
T1  - Preliminary studies of a micro-CT for a combined small animal PET/CT scanner
JF  - 2001 IEEE Nuclear Science Symposium Conference Record, Vol. 3
N2  - We are developing an X-ray computed tomography (CT) system which will be combined with a high resolution animal PET system. This permits acquisition of both molecular and anatomical images in a single machine. In particular the CT will also be utilized for the quantification of the animal PET data by providing accurate data for attenuation correction. A first prototype has been built using a commercially available plane silicon diode detector. A cone-beam reconstruction provides the images using the Feldkamp algorithm. First measurements with this system have been performed on a mouse. It could be shown that the CT setup fulfils all demands for a high quality image of the skeleton of the mouse. It is also suited for soft tissue measurements. To improve contrast and resolution and to acquire the X-ray energy further development of the system, especially the use of semiconductor detectors and iterative reconstruction algorithms are planned.
Y1  - 2002
SN  - 1082-3654
SP  - 1605
EP  - 1606
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Schmid, Georg
A1  - Wandrey, Christian
T1  - Preparation of cellodextrins and isolation of oligomeric side components and their characterization / Schmid, Georg ; Biselli, Manfred ; Wandrey, Christian
JF  - Analytical Biochemistry. 175 (1988)
Y1  - 1988
SN  - 0003-2697
SP  - 573
EP  - 583
ER  -