TY  - BOOK
A1  - Buyer, Christoph
A1  - Klein, Hartmut
A1  - Müller, Thomas
T1  - Änderung der Unternehmensform :  Handbuch zum Umwandlungs- und Umwandlungssteuerrecht
Y1  - 2010
SN  - 978-3-482-42258-4
PB  - Verl. Neue Wirtschafts-Briefe
CY  - Herne
ET  - 8. Aufl.
ER  - 
TY  - JOUR
A1  - Goldbach, Daniel
T1  - Fahrstabilitätserhöhung bei Gespannen durch Querverschiebung des Anhängerkupplungspunktes
JF  - mechatronik mobil
Y1  - 2010
SN  - 1867-7371
VL  - 2
IS  - 1
SP  - 23
EP  - 29
PB  - Expert Verlag
CY  - Renningen
ER  - 
TY  - CHAP
A1  - Goldbach, Daniel
T1  - Management der funktionalen Sicherheit einer Entwicklung eines sicherheitskritischen Systems im Bereich Nutzfahrzeuge
T2  - Commercial vehicle technology 2010 : proceedings of the 1st Commercial Vehicle Technology Symposium (CVT 2010), March 16 - 18, 2010, Kaiserslautern / K. Berns ... (ed.)
Y1  - 2010
SN  - 978-3-8322-9040-5
SP  - 487
EP  - 495
PB  - Shaker
CY  - Aachen
ER  - 
TY  - CHAP
A1  - Goldbach, Daniel
A1  - Ament, Christoph
ED  - Scharff, Peter
T1  - Stabilized reversing with vehicle trailer combinations
T2  - Crossing borders within the ABC : automation, biomedical engineering and computer science; proceedings; 55. IWK Internationales Wissenschaftliches Kolloquium; 13 - 17 September 2010, Ilmenau
Y1  - 2010
SN  - 978-3-938843-53-6
SP  - 352
EP  - 357
PB  - Verl. ISLE
CY  - Ilmenau
ER  - 
TY  - GEN
A1  - Alt, Helmut
T1  - Vom Studium der Kerntechnik zu Energie zwischen Wunsch und Wirklichkeit : [Vortrag : Verleihung der KTG-Ehrenmitgliedschaft, Berlin, November 2010]
Y1  - 2010
SP  - 1
EP  - 15
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Karl, W.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwab, H.
T1  - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli
JF  - Journal of biotechnology
N2  - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477.

This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.
Y1  - 2010
U6  - https://doi.org/10.1016/j.jbiotec.2010.09.947
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 150
IS  - 3
SP  - 408
EP  - 416
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Mussmann, Nina
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Novel proteases and means containing said proteases [Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 30
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Baumstark, Rebecca
A1  - Kluin, Cornelia
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Hellmuth, Hendrik
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
Y1  - 2010
SP  - 1
EP  - 30
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Novel proteases and compositions comprising these proteases [Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 31
PB  - Deutsches Patentamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
T1  - Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus pumilus [Offenlegungsschrift]
T1  - Detergents and cleaning agents containing proteases from bacillus pumilus [Europäische Patentschrift / Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 20
PB  - Deutsches Patentamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  -