TY - CHAP A1 - Steuer-Dankert, Linda A1 - Berg, Tobias A1 - Leicht-Scholten, Carmen T1 - Breaking the habit – new approaches in engineering education T2 - Proceedings of the 43rd Annual Conference of the European Society for Engineering Education N2 - Within the framework of the project a genderand diversity-oriented teaching evaluation and modern, media-supported blended learning approaches were used in order to achieve the intended goals. First research results of the literature and status quo analysis were already implemented and tested in newly designed teaching approaches, for example in a multidisciplinary introductory lecture of civil engineering at RWTH Aachen University. KW - teaching KW - engineering education KW - gender and diversity KW - best practice Y1 - 2015 SN - 978-2-87352-012-0 N1 - Annual Conference of the European Society for Engineering Education , Orléans , France , SEFI , 2015-06-29 - 2015-07-02 ER - TY - JOUR A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Scheja, Sabrina A1 - Wu, Chunsheng A1 - Keusgen, Michael A1 - Mewes, Dieter A1 - Schöning, Michael Josef T1 - DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer JF - Applied Materials & Interfaces N2 - Miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge favor the semiconductor field-effect platform as one of the most attractive approaches for the development of label-free DNA chips. In this work, a capacitive field-effect EIS (electrolyte–insulator–semiconductor) sensor covered with a layer-by-layer prepared, positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was used for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization. The negatively charged probe single-stranded DNA (ssDNA) molecules were electrostatically adsorbed onto the positively charged PAH layer, resulting in a preferentially flat orientation of the ssDNA molecules within the Debye length, thus yielding a reduced charge-screening effect and a higher sensor signal. Each sensor-surface modification step (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), reducing an unspecific adsorption by a blocking agent, incubation with noncomplementary DNA (ncDNA) solution) was monitored by means of capacitance–voltage and constant-capacitance measurements. In addition, the surface morphology of the PAH layer was studied by atomic force microscopy and contact-angle measurements. High hybridization signals of 34 and 43 mV were recorded in low-ionic strength solutions of 10 and 1 mM, respectively. In contrast, a small signal of 4 mV was recorded in the case of unspecific adsorption of fully mismatched ncDNA. The density of probe ssDNA and dsDNA molecules as well as the hybridization efficiency was estimated using the experimentally measured DNA immobilization and hybridization signals and a simplified double-layer capacitor model. The results of field-effect experiments were supported by fluorescence measurements, verifying the DNA-immobilization and hybridization event. Y1 - 2015 U6 - https://doi.org/10.1021/acsami.5b05146 VL - 36 IS - 7 SP - 20068 EP - 20075 PB - American Chemical Society CY - Washington, DC ER - TY - CHAP A1 - Krückel, Kai A1 - Nolden, Florian A1 - Ferrein, Alexander A1 - Scholl, Ingrid T1 - Intuitive visual teleoperation for UGVs using free-look augmented reality displays T2 - 2015 IEEE International Conference on Robotics and Automation (ICRA), Seattle, WA Y1 - 2015 U6 - https://doi.org/10.1109/ICRA.2015.7139809 SP - 4412 EP - 4417 ER - TY - JOUR A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Schöning, Michael Josef T1 - Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer JF - Nanoscale N2 - A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent–voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout. Y1 - 2015 U6 - https://doi.org/10.1039/C4NR07225A VL - 14 IS - 7 SP - 6143 EP - 6150 PB - Royal Society of Chemistry (RSC) CY - Cambridge ER - TY - CHAP A1 - Rahier, Michael A1 - Ritz, Thomas A1 - Wallenborn, Ramona T1 - Information and communication technology for integrated mobility concepts such as E-carsharing T2 - E-Mobility in Europe : trends and good practice N2 - During the past decade attitude towards sharing things has changed extremely. Not just personal data is shared (e.g. in social networks) but also mobility. Together with the increased ecological awareness of the recent years, new mobility concepts have evolved. E-carsharing has become a symbol for these changes of attitude. The management of a shared car fleet, the energy management of electric mobility and the management of various carsharing users with individual likes and dislikes are just some of the major challenges of e-carsharing. Weaving it into integrated mobility concepts, this raises complexity even further. These challenges can only be overcome by an appropriate amount of well-shaped information available at the right place and time. In order to gather, process and share the required information, fleet cars have to be equipped with modern information and communication technology (ICT) and become so-called fully connected cars. Ensuring the usability of these ICT systems is another challenge that is often neglected, even though it is usability that makes carsharing comfortable, attractive and supports users’ new attitudes. By means of an integrated and consistent concept for human-machine interaction (HMI), the usability of such systems can be raised tremendously. KW - Information and communication technology KW - Fully connected car KW - E-carsharing KW - Mobility management KW - Integrated mobility Y1 - 2015 SN - 978-3-319-13193-1 U6 - https://doi.org/10.1007/978-3-319-13194-8_17 SP - 311 EP - 326 PB - Springer CY - Cham [u.a.] ER - TY - CHAP A1 - Anthrakidis, Anette A1 - Herrmann, Ulf A1 - Schorn, Christian A1 - Schwarzer, Klemens A1 - Wedding, Philipp A1 - Weis, Fabian T1 - Development and Testing of a Novel Method for the Determination of the Efficiency of Concentrating Solar Thermal Collectors T2 - Conference Proceedings Solar World Congress 2015, Daegu, Korea, 08 – 12 November 2015 Y1 - 2015 ER - TY - JOUR A1 - Al-Kaidy, Huschyar A1 - Duwe, Anna A1 - Huster, Manuel A1 - Muffler, Kai A1 - Schlegel, Christin A1 - Tim, Sieker A1 - Stadtmüller, Ralf A1 - Tippkötter, Nils A1 - Ulber, Roland T1 - Biotechnology and bioprocess engineering – from the first ullmann's article to recent trends JF - ChemBioEng Reviews N2 - For several thousand years, biotechnology and its associated technical processes have had a great impact on the development of mankind. Based on empirical methods, in particular for the production of foodstuffs and daily commodities, these disciplines have become one of the most innovative future issues. Due to the increasing detailed understanding of cellular processes, production strains can now be optimized. In combination with modern bioprocesses, a variety of bulk and fine chemicals as well as pharmaceuticals can be produced efficiently. In this article, some of the current trends in biotechnology are discussed. Y1 - 2015 U6 - https://doi.org/10.1002/cben.201500008 VL - 2 IS - 3 SP - 175 EP - 184 PB - Wiley CY - Weinheim ER - TY - CHAP A1 - Höfler, M. A1 - Kneer, R. A1 - Groß, Rolf Fritz A1 - Kehrmann, K. ED - Vorobieff, P. T1 - Chemical determination of oxygen transfer rates, transfer efficiencies and interphases evoked by aeration elements for liquid flows T2 - Computational Methods in Multiphase Flow VIII. - (WIT Transactions on Engineering Sciences ; Volume 89) Y1 - 2015 SN - 978-1-84564-946-3 (Print-Ausgabe) SN - 978-1-84564-947-0 (Online-Ausgabe) SN - 1746-4471 SP - 89 EP - 101 PB - WIT Press CY - Southampton ER - TY - JOUR A1 - Henderson, Colin J. A1 - Mclaughlin, Lesley A. A1 - Scheer, Nico A1 - Stanley, Lesley A. A1 - Wolf, C. Roland T1 - Cytochrome b5 Is a Major Determinant of Human Cytochrome P450 CYP2D6 and CYP3A4 Activity In Vivo s JF - Molecular Pharmacology Y1 - 2015 U6 - https://doi.org/10.1124/mol.114.097394 SN - 1521-0111 VL - 87 IS - 4 SP - 733 EP - 739 PB - ASPET CY - Bethesda ER - TY - CHAP A1 - Wolf, Martin R. A1 - Lenz, Laura L. T1 - The economic effectiveness of serious games in the healthcare environment : application and evaluation of the Comparative Transformation Model (CTM) T2 - IEEE 3rd International Conference on Serious Games and Applications for Health : SeGAH 2014 ; Rio de Janeiro, Brazil, May 14 - 16 Y1 - 2015 SN - 978-1-4799-4823-9 U6 - https://doi.org/10.1109/SeGAH.2014.7067089 SP - 135 EP - 142 PB - IEEE [u.a.] CY - Piscataway, NJ ER -