TY  - JOUR
A1  - Engelmann, Ulrich M.
A1  - Simsek, Beril
A1  - Shalaby, Ahmed
A1  - Krause, Hans-Joachim
T1  - Key contributors to signal generation in frequency mixing magnetic detection (FMMD): an in silico study
JF  - Sensors
N2  - Frequency mixing magnetic detection (FMMD) is a sensitive and selective technique to detect magnetic nanoparticles (MNPs) serving as probes for binding biological targets. Its principle relies on the nonlinear magnetic relaxation dynamics of a particle ensemble interacting with a dual frequency external magnetic field. In order to increase its sensitivity, lower its limit of detection and overall improve its applicability in biosensing, matching combinations of external field parameters and internal particle properties are being sought to advance FMMD. In this study, we systematically probe the aforementioned interaction with coupled Néel–Brownian dynamic relaxation simulations to examine how key MNP properties as well as applied field parameters affect the frequency mixing signal generation. It is found that the core size of MNPs dominates their nonlinear magnetic response, with the strongest contributions from the largest particles. The drive field amplitude dominates the shape of the field-dependent response, whereas effective anisotropy and hydrodynamic size of the particles only weakly influence the signal generation in FMMD. For tailoring the MNP properties and parameters of the setup towards optimal FMMD signal generation, our findings suggest choosing large particles of core sizes dc > 25 nm nm with narrow size distributions (σ < 0.1) to minimize the required drive field amplitude. This allows potential improvements of FMMD as a stand-alone application, as well as advances in magnetic particle imaging, hyperthermia and magnetic immunoassays.
KW  - key performance indicators
KW  - magnetic biosensing
KW  - coupled Néel–Brownian relaxation dynamics
KW  - frequency mixing magnetic detection
KW  - magnetic relaxation
KW  - micromagnetic simulation
KW  - magnetic nanoparticles
Y1  - 2024
U6  - https://doi.org/10.3390/s24061945
SN  - 1424-8220
N1  - This article belongs to the Special Issue "Advances in Magnetic Sensors and Their Applications"
VL  - 24
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Ermelenko, Y.
A1  - Yoshinobu, T
A1  - Mourzina, Y.
A1  - Levichev, S.
A1  - Furuichi, K.
A1  - Vlasov, Y.
A1  - Schöning, Michael Josef
A1  - Iwasaki, H.
T1  - Photocurable membranes for ion-selective light-addressable potentiometric sensors
JF  - Sensors and Actuators B. 85 (2002), H. 1-2
Y1  - 2002
SN  - 0925-4005
SP  - 79
EP  - 85
ER  - 
TY  - JOUR
A1  - Ermelenko, Y.
A1  - Yoshinobu, T.
A1  - Mourzina, Y.
A1  - Furuichi, K.
A1  - Iwasaki, H.
A1  - Vlasov, Y.
A1  - Schöning, Michael Josef
T1  - Technology of photocurable polymeric membranes for integrated LAPS
JF  - Proceedings of the 5th East Asian Conference on Chemical Sensors; the 33rd Chemical Sensor Symposium : December 4 - 7, 2001, Huis Ten Bosch, Sasebo-shi, Nagasaki, Japan / Japan Association of Chemical Sensors; the Electrochemical Society of Japan
Y1  - 2001
N1  - Chemical sensors; 17.2001 Suppl. B; East Asian Conference on Chemical Sensors ; (5, 2001; Nagasaki)
SP  - 66
EP  - 68
PB  - Japan Association of Chemical Sensors
CY  - Kasuga, Fukuoka-ken
ER  - 
TY  - JOUR
A1  - Ermelenko, Y.
A1  - Yoshinobu, T.
A1  - Mourzina, Y.
A1  - Furuichi, K.
A1  - Levichev, S.
A1  - Vlasov, Y.
A1  - Schöning, Michael Josef
A1  - Iwasaki, H.
T1  - Lithium sensor based on the laser scanning semiconductor transducer
JF  - Analytica Chimica Acta. 459 (2002), H. 1
Y1  - 2002
SN  - 0378-4304
SP  - 1
EP  - 9
ER  - 
TY  - JOUR
A1  - Ermelenko, Y.
A1  - Yoshinobu, T.
A1  - Mourzina, Y.
A1  - Schöning, Michael Josef
A1  - Furuichi, K.
A1  - Levichev, S.
A1  - Vlasov, Y.
A1  - Iwasaki, H.
T1  - The double K+/Ca2+ sensor based on laser scanned silicon transducer (LSST) for multicomponent analysis
JF  - Talanta. 59 (2003), H. 4
Y1  - 2003
SN  - 0039-9140
SP  - 785
EP  - 795
ER  - 
TY  - JOUR
A1  - Ermelenko, Y.
A1  - Yoshinobu, T.
A1  - Mourzina, Y.
A1  - Schöning, Michael Josef
A1  - Vlasov, Y.
A1  - Iwasaki, H.
T1  - The hybrid K+/Ca2+ sensor based on laser scanned silicon transducer for multi-component analysis
JF  - Proceedings of ICAS 2001, IUPAC [3rd] International Congress on Analytical Sciences 2001 : August 6 - 10, 2001, Waseda University, Tokyo
Y1  - 2002
N1  - International Congress on Analytical Sciences <2001, Tokyo> ; International Union of Pure and Applied Chemistry. Pure and applied chemistry ; 73,10. ; Analytical sciences ; 17.2001, special issue
SP  - i777
EP  - i780
PB  - Japan Society for Analytical Chemistry
CY  - Tokyo
ER  - 
TY  - CHAP
A1  - Ermelenko, Y.
A1  - Yoshinobu, T.
A1  - Mourzina, Y.
A1  - Schöning, Michael Josef
A1  - Vlasov, Y.
A1  - Iwasaki, H.
T1  - A multisensor based on laser scanned silicon transducer (LSST): development and properties
T2  - Eurosensors XVII : the 17th European Conference on Solid-State Transducers ; University of Minho, Guimarães, Portugal, September 21 - 24, 2003
Y1  - 2003
SP  - 72
EP  - 73
ER  - 
TY  - JOUR
A1  - Ermolenko, Y. E.
A1  - Yoshinobu, T.
A1  - Mourzina, Y. G.
A1  - Vlasov, Y. G.
A1  - Schöning, Michael Josef
A1  - Iwasaki, H.
T1  - Laserscanned transducer (LSST) as a multisensor system
JF  - Sensors and Actuators B. 103 (2004), H. 1-2
Y1  - 2004
SN  - 0925-4005
SP  - 457
EP  - 462
ER  - 
TY  - JOUR
A1  - Ermolenko, Y.E.
A1  - Vlasov, Y.G.
A1  - Kolodnikov, V.V.
A1  - Shabaldkin, D. A.
A1  - Kloock, Joachim P.
A1  - Schöning, Michael Josef
T1  - Diffusion of radioactive tracers (204Tl, 110mAg) and ionic conductivity in membrane materials for the chemical sensors
JF  - Advances in nuclear and radiochemistry : extended abstracts of papers presented at the Sixth International Conference on Nuclear and Radiochemistry (NRC-6), 29 August to 3 September 2004, Aachen, Germany ; in cooperation with University of Cologne, GDCh, FECS, OECD-NEA and IAEA / Syed M. Qaim ... (eds.).
Y1  - 2004
SN  - 3893363629
N1  - Schriften des Forschungszentrums Jülich Reihe Allgemeines und Interdisziplinäres ; 3. International Conference on Nuclear and Radiochemistry ; (6, 2004, Aachen)
SP  - 483
EP  - 485
PB  - Forschungszentrum Jülich, Zentralbibliothek
CY  - Jülich
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
JF  - Frontiers in Microbiology
N2  - The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates.
Y1  - 2022
U6  - https://doi.org/10.3389/fmicb.2022.1017978
SN  - 1664-302X
VL  - 2022
IS  - 13
PB  - Frontiers
CY  - Lausanne
ER  -