TY  - JOUR
A1  - Jordan, Sabine D.
A1  - Krüger, Markus
A1  - Willmes, Diana M.
A1  - Redemann, Nora
A1  - Wunderlich, F. Thomas
A1  - Brönneke, Hella S.
A1  - Merkwirth, Carsten
A1  - Kashkar, Hamid
A1  - Olkkonen, Vesa M.
A1  - Böttger, Thomas
A1  - Braun, Thomas
A1  - Seibler, Jost
A1  - Brüning, Jens C.
T1  - Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism
JF  - Nature Cell Biology
N2  - The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.
Y1  - 2011
U6  - http://dx.doi.org/10.1038/ncb2211
SN  - 1465-7392
VL  - 13
IS  - 4
SP  - 434
EP  - 446
PB  - Nature
CY  - New York
ER  - 
TY  - JOUR
A1  - Ratke, Lorenz
A1  - Milow, Barbara
A1  - Lisinski, Susanne
A1  - Hoepfner, Sandra
T1  - On an effect of fine ceramic particles on the structure of aerogels
JF  - Microgravity science and technology
Y1  - 2014
U6  - http://dx.doi.org/10.1007/s12217-014-9380-2
SN  - 0938-0108 ; 1875-0494
VL  - 26
SP  - 103
EP  - 110
PB  - Springer Nature
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Noll, T.
A1  - Wandrey, C.
T1  - On-line biomass monitoring of immobilized hybridoma cells by dielectrical measurements / Noll, T. ; Biselli, M. ; Wandrey, C.
JF  - Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo
Y1  - 1997
SN  - 0-7923-4321-2
SP  - 289
EP  - 294
PB  - Kluwer
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Van der Pol, Jens J.
A1  - Joksch, Burkhard
A1  - Galgens, Jochem
T1  - On-line control of an immobilized hybridoma culture with multi-channel flow injektion analysis / Jens J. van der Pol, Burkhard Joksch, Jochem Galgens, Manfred Biselli, Cornelis D. de Gooijer, Johannes Tramper and Christian Wandrey
JF  - Journal of Biotechnology. 43 (1995), H. 3
Y1  - 1995
SN  - 0168-1656
N1  - 3. Autor vermutlich: Jochen Gätgens
SP  - 229
EP  - 242
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Van der Pol, Jens J.
A1  - Machnik, Marc
A1  - Portela-Klein, Theresa
T1  - On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells / Jens J. van der Pol, Marc Machnik, Manfred Biselli, Theresa Portela-Klein, Cornelis D. de Gooijer, Johannes Tramper und Christian Wandrey
JF  - Cytotechnology. 24 (1997), H. 1
Y1  - 1997
SN  - 0920-9069
SP  - 19
EP  - 30
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Van der Pol, Jens J.
A1  - Joksch, B.
A1  - Spohn, U.
T1  - On-line monitoring and control of glucose, glutamine, lactate and ammonium during a high-cell-density cultivation of hybridoma cells / van der Pol, Jens J. ; Joksch, B. ; Spohn, U. ; Biselli, M. ; Wandrey, C.
JF  - Proceedings of the sixth international meeting of the Japanese Association for Animal Cell Technology [JAACT 93]: Nagoya, Japan, November 9 - 12, 1993 / ed. by T. Kobayashi
Y1  - 1994
SN  - 0-7923-3156-7
N1  - International meeting of the Japanese Association for Animal Cell Technology, JAACT 93 ; (6 : ; 1993.11.09-12 : ; Nagoya, Japan) ; Animal cell technology, basic and applied aspects ; 6
SP  - 167
EP  - 170
PB  - Springer Netherland
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Van der Pol, Jens J.
A1  - Spohn, Uwe
A1  - Eberhardt, Rolf
T1  - On-line monitoring of an animal cell culture with multi-channel flow injection analysis / van der Pol, Jens J.; Spohn, Uwe ; Eberhardt, Rolf ; Gätgens, Jochen ; Biselli, Manfred ; Wandrey, Christian ; Tramper, Johannes
JF  - Journal of Biotechnology. 37 (1994), H. 3
Y1  - 1994
SN  - 0168-1656
SP  - 253
EP  - 264
ER  - 
TY  - JOUR
A1  - Hough, Lindsay B.
A1  - Nalwalk, Julia W.
A1  - Ding, Xinxin
A1  - Scheer, Nico
T1  - Opioid Analgesia in P450 Gene Cluster Knockout Mice: A Search for Analgesia-Relevant Isoforms
JF  - Drug Metabolism and Disposition
Y1  - 2015
U6  - http://dx.doi.org/10.1124/dmd.115.065490
SN  - 1521-009x
VL  - 43
IS  - 9
SP  - 1326
EP  - 1330
ER  - 
TY  - JOUR
A1  - Niedermeier, Jana
A1  - Penner, Crystal
A1  - Usherovich, Samuel
A1  - Bélanger-Champagne, Camille
A1  - Paulßen, Elisabeth
A1  - Cornelia, Hoehr
T1  - Optical Fibers as Dosimeter Detectors for Mixed Proton/Neutron Fields - A Biological Dosimeter
JF  - electronics
N2  - In recent years, proton therapy has gained importance as a cancer treatment modality due to its conformality with the tumor and the sparing of healthy tissue. However, in the interaction of the protons with the beam line elements and patient tissues, potentially harmful secondary neutrons are always generated. To ensure that this neutron dose is as low as possible, treatment plans could be created to also account for and minimize the neutron dose. To monitor such a treatment plan, a compact, easy to use, and inexpensive dosimeter must be developed that not only measures the physical dose, but which can also distinguish between proton and neutron contributions. To that end, plastic optical fibers with scintillation materials (Gd₂O₂S:Tb, Gd₂O₂S:Eu, and YVO₄:Eu) were irradiated with protons and neutrons. It was confirmed that sensors with different scintillation materials have different sensitivities to protons and neutrons. A combination of these three scintillators can be used to build a detector array to create a biological dosimeter.
KW  - biological dosimeter
KW  - Bragg peak
KW  - relative dosimetry
KW  - optical fibers
KW  - proton therapy
KW  - protons
KW  - neutrons
Y1  - 2023
U6  - http://dx.doi.org/10.3390/electronics12020324
SN  - 2079-9292
N1  - This article belongs to the Special Issue "Applications of Optical Fiber Sensors"
VL  - 12
IS  - 2
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Srivastava, A.
A1  - Singh, V.
A1  - Aggarwal, P.
A1  - Schneeweiss, F.
A1  - Scherer, Ulrich W.
A1  - Friedrich, W.
T1  - Optical studies of insulating polymers for radiation dose monitoring
JF  - Indian Journal of Pure & Applied Physics
Y1  - 2010
SN  - 0019-5596
N1  - Special Issue: SI
VL  - 48
IS  - 11
SP  - 782
EP  - 786
ER  -