TY  - JOUR
A1  - Kotter, Michael
A1  - Riekert, L.
T1  - Beeinflussung der Aktivität und Selektivität von Tränkkatalysatoren durch die topographische Verteilung der Aktivkomponente
JF  - Verfahrenstechnik  : VT ; Informationen für Planung, Bau und Betrieb von Apparaten und Anlagen. 17 (1983)
Y1  - 1983
SN  - 0049-5948
N1  - 2. ISSN: 0175-5315 , 3. ISSN: 0341-2644
SP  - 639
EP  - 658
ER  - 
TY  - JOUR
A1  - Burger, René
A1  - Lindner, Simon
A1  - Rumpf, Jessica
A1  - Do, Xuan Tung
A1  - Diehl, Bernd W.K.
A1  - Rehahn, Matthias
A1  - Monakhova, Yulia
A1  - Schulze, Margit
T1  - Benchtop versus high field NMR: Comparable performance found for the molecular weight determination of lignin
JF  - Journal of Pharmaceutical and Biomedical Analysis
N2  - Lignin is a promising renewable biopolymer being investigated worldwide as an environmentally benign substitute of fossil-based aromatic compounds, e.g. for the use as an excipient with antioxidant and antimicrobial properties in drug delivery or even as active compound. For its successful implementation into process streams, a quick, easy, and reliable method is needed for its molecular weight determination. Here we present a method using 1H spectra of benchtop as well as conventional NMR systems in combination with multivariate data analysis, to determine lignin’s molecular weight (Mw and Mn) and polydispersity index (PDI). A set of 36 organosolv lignin samples (from Miscanthus x giganteus, Paulownia tomentosa and Silphium perfoliatum) was used for the calibration and cross validation, and 17 samples were used as external validation set. Validation errors between 5.6% and 12.9% were achieved for all parameters on all NMR devices (43, 60, 500 and 600 MHz). Surprisingly, no significant difference in the performance of the benchtop and high-field devices was found. This facilitates the application of this method for determining lignin’s molecular weight in an industrial environment because of the low maintenance expenditure, small footprint, ruggedness, and low cost of permanent magnet benchtop NMR systems.
KW  - NMR
KW  - PLS-regression
KW  - Molecular weight determination
KW  - Chemometrics
KW  - Biomass
Y1  - 2022
SN  - 0731-7085
U6  - http://dx.doi.org/10.1016/j.jpba.2022.114649
VL  - 212
IS  - Article number: 114649
PB  - Elsevier
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Iding, Hans
A1  - Dünnwald, Thomas
A1  - Greiner, Lasse
A1  - Liese, Andreas
A1  - Müller, Michael
A1  - Siegert, Petra
A1  - Grötzinger, Joachim
A1  - Demir, Ayhan S.
A1  - Pohl, Martina
T1  - Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones
JF  - Chemistry - a European journal
Y1  - 2000
SN  - 1521-3765 (E-Journal); 0947-6539 (Print)
VL  - Vol. 6
IS  - Iss. 8
SP  - 1483
EP  - 1495
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Groebel, Simone
A1  - Schuhmacher, K.
A1  - Spelthahn, Heiko
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Bestimmung der metabolischen Aktivität von Mikroorganismen während des Biogasbildungsprozesses
JF  - 9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]
Y1  - 2009
SN  - 978-3-941298-44-6
N1  - Dresdner Sensor-Symposium ; (9, 2009, Dresden)
SP  - 201
EP  - 204
PB  - TUDpress
CY  - Dresden
ER  - 
TY  - PAT
A1  - Bankowski, Bernhard
A1  - Hoffmann, Daniele
A1  - Wadle, Armin
A1  - Siegert, Petra
A1  - Sättler, Andrea
A1  - Gerke, Thomas
T1  - Beta-Glucuronidase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - Beta-Glucuronidase inhibitors for use in deodorants and antiperspirants [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2003
SP  - 1
EP  - 24
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - JOUR
A1  - Merschenz-Quack, Angelika
T1  - Betriebsanweisungen beim Umgang mit Gefahrstoffen
JF  - Elektro-Praktiker : Fachzeitschrift für Handwerk und Industrie
Y1  - 1991
SN  - 0013-5569
VL  - Bd. 45
IS  - H. 10
SP  - 644
EP  - 649
ER  - 
TY  - CHAP
A1  - Muffler, Kai
A1  - Poth, Sabastian
A1  - Sieker, Tim
A1  - Tippkötter, Nils
A1  - Ulber, Roland
A1  - Sell, Dieter
ED  - Moo-Young, Murray
T1  - Bio-feedstocks
T2  - Comprehensive biotechnology : principles and practices in industry, agcriculture, medicine and the environment. Volume 2: Engineering fundamentals of biotechnology
Y1  - 2011
SN  - 978-0-444-53352-4
U6  - http://dx.doi.org/10.1016/B978-0-08-088504-9.00088-X
SP  - 93
EP  - 101
PB  - Elsevier
CY  - Amsterdam
ET  - 2. edition
ER  - 
TY  - CHAP
A1  - Tippkötter, Nils
A1  - Schünemann, V.
A1  - Christmann, R.
A1  - Pasteur, A.
A1  - Schweizer, J.
A1  - Ulber, R.
ED  - Beckmann, Dieter
T1  - Bioaffinity Layering magnetisierbarer Mikro- und Nanopartikel
T2  - Technische Systeme für die Lebenswissenschaften : 14. Heiligenstädter Kolloquium, Heilbad Heiligenstadt, 22.09. - 24.09.2008
Y1  - 2008
SN  - 978-3-00-025695-0
SP  - 97
EP  - 98
CY  - Heiligenstadt
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Netz, Daili Jacqueline Aguilar
A1  - Pohl, Regula
A1  - Beck-Sickinger, Annette G.
T1  - Biochemical characterisation and genetic analysis of aureocin A53, a new, atypical bacteriocin from Staphylococcus aureus / Netz, Daili Jacqueline Aguilar ; Pohl, Regula ; Beck-Sickinger, Annette G. ; Selmer, Thorsten ; Pierik, Antonio J. ;  Carmo de Frei
JF  - Journal of Molecular Biology. 319 (2002), H. 3
Y1  - 2002
SN  - 0022-2836
SP  - 745
EP  - 756
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Kohn, Sophie
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ
JF  - FEBS Open Bio
N2  - Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications.
KW  - Bacillaceae
KW  - Biotechnological application
KW  - Broad pH spectrum
KW  - Subtilases
KW  - Subtilisin
Y1  - 2023
U6  - http://dx.doi.org/10.1002/2211-5463.13701
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 13
IS  - 11
SP  - 2035
EP  - 2046
PB  - Wiley
CY  - Hoboken, NJ
ER  -