TY - JOUR A1 - Tippkötter, Nils A1 - Stückmann, Henning A1 - Kroll, Stephen A1 - Winkelmann, Gunda A1 - Noack, Udo A1 - Scheper, Thomas A1 - Ulber, Roland T1 - A semi-quantitative dipstick assay for microcystin JF - Analytical and Bioanalytical Chemistry N2 - An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l−1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels. Y1 - 2009 U6 - http://dx.doi.org/10.1007/s00216-009-2750-8 SN - 1618-2650 VL - 394 IS - 3 SP - 863 EP - 869 PB - springer CY - Berlin ER - TY - JOUR A1 - Ulber, Roland A1 - Tippkötter, Nils T1 - Nitratfreie Molke JF - Rundschau für Fleischhygiene und Lebensmittelüberwachung Y1 - 2009 IS - 4 SP - 150 EP - 152 ER - TY - JOUR A1 - Henderson, Colin J. A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Advances in the generation of mouse models to elucidate the pathways of drug metabolism in rodents and man JF - Expert Review of Clinical Pharmacology Y1 - 2009 U6 - http://dx.doi.org/10.1586/17512433.2.2.105 SN - 1751-2441 VL - 2 IS - 2 SP - 105 EP - 109 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Stanley, Lesley A. A1 - Horsburgh, Brian C. A1 - Ross, Jillian A1 - Scheer, Nico A1 - Wolf, C. Roland T1 - Drug transporters: Gatekeepers controlling access of xenobiotics to the cellular interior JF - Drug Metabolism Reviews Y1 - 2009 U6 - http://dx.doi.org/10.1080/03602530802605040 SN - 1097-9883 VL - 41 IS - 1 SP - 27 EP - 65 PB - Taylor & Francis CY - London ER - TY - JOUR A1 - Balakrishnan, Karthikeyan A1 - Andrei-Selmer, Luminita-Cornelia A1 - Selmer, Thorsten A1 - Bacher, Michael A1 - Dodel, Richard T1 - Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β JF - Journal of Alzheimer's Disease Y1 - 2010 SN - 1387-2877 VL - 20 IS - 1 SP - 135 EP - 143 ER - TY - JOUR A1 - Kowollik, S. A1 - Schnitzler, Thomas A1 - Biselli, Manfred A1 - Krueger, R. A1 - Zang, Werner A1 - Peuscher, A. A1 - Schillberg, S. A1 - Fischer, R. T1 - Die Rolle des Respirationsquotienten in der Zellkulturfermentation JF - Chemie Ingenieur Technik Y1 - 2010 SN - Chemie Ingenieur Tec N1 - Special Issue: ProcessNet-Jahrestagung 2010 und 28. Jahrestagung der Biotechnologen VL - 82 IS - 9 SP - 1505 EP - 1506 ER - TY - JOUR A1 - Biselli, Manfred A1 - Bäcker, Matthias A1 - Poghossian, Arshak A1 - Schöning, Michael Josef A1 - Schnitzler, Thomas A1 - Zang, Werner A1 - Wagner, P. T1 - Entwicklung eines modularen festkörperbasierten Sensorsystems für die Überwachung von Zellkulturfermenationen JF - Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vorträge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in Nürnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA) Y1 - 2010 SN - 978-3-8007-3260-9 N1 - Fachtagung Sensoren und Messsysteme 15, 2010, Nürnberg ; Gesellschaft Mess- und Automatisierungstechnik SP - 688 EP - 691 PB - VDE Verlag CY - Berlin ER - TY - JOUR A1 - Claessen, O. A1 - Grefen, Dana A1 - Mang, Thomas A1 - Dikland, H. G. A1 - Dikland, H. G. A1 - Duin, M. van T1 - Helle Fensterprofilmaterialien : Alterungsverhalten auf Basis von peroxidisch vernetztem EPDM JF - Kautschuk, Gummi, Kunststoffe : KGK Y1 - 2010 SN - 0948-3276 VL - 63 IS - 9 SP - 350 EP - 360 ER - TY - JOUR A1 - Srivastava, A. A1 - Singh, V. A1 - Aggarwal, P. A1 - Schneeweiss, F. A1 - Scherer, Ulrich W. A1 - Friedrich, W. T1 - Optical studies of insulating polymers for radiation dose monitoring JF - Indian Journal of Pure & Applied Physics Y1 - 2010 SN - 0019-5596 N1 - Special Issue: SI VL - 48 IS - 11 SP - 782 EP - 786 ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - http://dx.doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER -