TY - CHAP A1 - Duong, Minh Tuan A1 - Seifarth, Volker A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard A1 - Staat, Manfred ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Growth Modelling Promoting Mechanical Stimulation of Smooth Muscle Cells of Porcine Tubular Organs in a Fibrin-PVDF Scaffold T2 - Biological, Physical and Technical Basics of Cell Engineering N2 - Reconstructive surgery and tissue replacements like ureters or bladders reconstruction have been recently studied, taking into account growth and remodelling of cells since living cells are capable of growing, adapting, remodelling or degrading and restoring in order to deform and respond to stimuli. Hence, shapes of ureters or bladders and their microstructure change during growth and these changes strongly depend on external stimuli such as training. We present the mechanical stimulation of smooth muscle cells in a tubular fibrin-PVDFA scaffold and the modelling of the growth of tissue by stimuli. To this end, mechanotransduction was performed with a kyphoplasty balloon catheter that was guided through the lumen of the tubular structure. The bursting pressure was examined to compare the stability of the incubated tissue constructs. The results showed the significant changes on tissues with training by increasing the burst pressure as a characteristic mechanical property and the smooth muscle cells were more oriented with uniformly higher density. Besides, the computational growth models also exhibited the accurate tendencies of growth of the cells under different external stimuli. Such models may lead to design standards for the better layered tissue structure in reconstructing of tubular organs characterized as composite materials such as intestines, ureters and arteries. KW - Mechanical simulation KW - Growth modelling KW - Ureter KW - Bladder KW - Reconstruction Y1 - 2018 SN - 978-981-10-7904-7 U6 - https://doi.org/10.1007/978-981-10-7904-7_9 SP - 209 EP - 232 PB - Springer CY - Singapore ER - TY - CHAP A1 - Duffner, Markus A1 - Moorkamp, Wilfried A1 - Peterson, Leif Arne A1 - Uibel, Thomas ED - Kuhlmann, Ulrike T1 - Untersuchungen zur Tragfähigkeit und Steifigkeit eines neuartigen Wandelements in Holzbauweisen T2 - Doktorandenkolloquium Holzbau Forschung + Praxis : Stuttgart, 08./09. März 2018 Y1 - 2018 SP - 131 EP - 139 PB - Universität Stuttgart, Institut für Konstruktion und Entwurf CY - Stuttgart ER - TY - JOUR A1 - Druckenmüller, Katharina A1 - Günther, Klaus A1 - Elbers, Gereon T1 - Near-infrared spectroscopy (NIRS) as a tool to monitor exhaust air from poultry operations JF - Science of the Total Environment N2 - Intensive poultry operation systems emit a considerable volume of inorganic and organic matter in the surrounding environment. Monitoring cleaning properties of exhaust air cleaning systems and to detect small but significant changes in emission characteristics during a fattening cycle is important for both emission and fattening process control. In the present study, we evaluated the potential of near-infrared spectroscopy (NIRS) combined with chemometric techniques as a monitoring tool of exhaust air from poultry operation systems. To generate a high-quality data set for evaluation, the exhaust air of two poultry houses was sampled by applying state-of-the-art filter sampling protocols. The two stables were identical except for one crucial difference, the presence or absence of an exhaust air cleaning system. In total, twenty-one exhaust air samples were collected at the two sites to monitor spectral differences caused by the cleaning device, and to follow changes in exhaust air characteristics during a fattening period. The total dust load was analyzed by gravimetric determination and included as a response variable in multivariate data analysis. The filter samples were directly measured with NIR spectroscopy. Principal component analysis (PCA), linear discriminant analysis (LDA), and factor analysis (FA) were effective in classifying the NIR exhaust air spectra according to fattening day and origin. The results indicate that the dust load and the composition of exhaust air (inorganic or organic matter) substantially influence the NIR spectral patterns. In conclusion, NIR spectroscopy as a tool is a promising and very rapid way to detect differences between exhaust air samples based on still not clearly defined circumstances triggered during a fattening period and the availability of an exhaust air cleaning system. Y1 - 2018 U6 - https://doi.org/10.1016/j.scitotenv.2018.02.072 SN - 0048-9697 VL - 630 SP - 536 EP - 543 PB - Elsevier CY - Amsterdam ER - TY - RPRT A1 - Drescher, Hans Paul T1 - The irreversible thermodynamic’s theorem of minimum entropy production applied to the laminar and the turbulent Couette flow N2 - Analyzing thermodynamic non-equilibrium processes, like the laminar and turbulent fluid flow, the dissipation is a key parameter with a characteristic minimum condition. That is applied to characterize laminar and turbulent behaviour of the Couette flow, including its transition in both directions. The Couette flow is chosen as the only flow form with constant shear stress over the flow profile, being laminar, turbulent or both. The local dissipation defines quantitative and stable criteria for the transition and the existence of turbulence. There are basic results: The Navier Stokes equations cannot describe the experimental flow profiles of the turbulent Couette flow. But they are used to quantify the dissipation of turbulent fluctuation. The dissipation minimum requires turbulent structures reaching maximum macroscopic dimensions, describing turbulence as a “non-local” phenomenon. At the transition the Couette flow profiles and the shear stress change by a factor ≅ 5 due to a change of the “apparent” turbulent viscosity by a calculated factor ≅ 27. The resulting difference of the laminar and the turbulent profiles results in two different Reynolds numbers and different loci of transition, which are identified by calculation. KW - Turbulence KW - Transition KW - Minimum dissipation Y1 - 2018 ER - TY - JOUR A1 - Ditzhaus, Marc A1 - Gaigall, Daniel T1 - A consistent goodness-of-fit test for huge dimensional and functional data JF - Journal of Nonparametric Statistics N2 - A nonparametric goodness-of-fit test for random variables with values in a separable Hilbert space is investigated. To verify the null hypothesis that the data come from a specific distribution, an integral type test based on a Cramér-von-Mises statistic is suggested. The convergence in distribution of the test statistic under the null hypothesis is proved and the test's consistency is concluded. Moreover, properties under local alternatives are discussed. Applications are given for data of huge but finite dimension and for functional data in infinite dimensional spaces. A general approach enables the treatment of incomplete data. In simulation studies the test competes with alternative proposals. KW - Cramér-von-Mises statistic KW - separable Hilbert space KW - huge dimensional data KW - functional data Y1 - 2018 U6 - https://doi.org/10.1080/10485252.2018.1486402 SN - 1029-0311 VL - 30 IS - 4 SP - 834 EP - 859 PB - Taylor & Francis CY - Abingdon ER - TY - JOUR A1 - Digel, Ilya A1 - Wehlitz, V. A1 - Kayser, Peter A1 - Figiel-Lange, A. A1 - Bassam, R. A1 - Rundstedt, F. von T1 - Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids JF - Plant Pathology N2 - Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach. Y1 - 2018 U6 - https://doi.org/10.1111/ppa.12750 SN - 1365-3059 VL - 67 IS - 2 SP - 358 EP - 365 PB - Wiley CY - Oxford ER - TY - CHAP A1 - Digel, Ilya A1 - Akimbekov, Nuraly S. A1 - Kistaubayeva, Aida A1 - Zhubanova, Azhar A. ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Microbial Sampling from Dry Surfaces: Current Challenges and Solutions T2 - Biological, Physical and Technical Basics of Cell Engineering N2 - Sampling of dry surfaces for microorganisms is a main component of microbiological safety and is of critical importance in many fields including epidemiology, astrobiology as well as numerous branches of medical and food manufacturing. Aspects of biofilm formation, analysis and removal in aqueous solutions have been thoroughly discussed in literature. In contrast, microbial communities on air-exposed (dry) surfaces have received significantly less attention. Diverse surface sampling methods have been developed in order to address various surfaces and microbial groups, but they notoriously show poor repeatability, low recovery rates and suffer from lack of mutual consistency. Quantitative sampling for viable microorganisms represents a particular challenge, especially on porous and irregular surfaces. Therefore, it is essential to examine in depth the factors involved in microorganisms’ recovery efficiency and accuracy depending on the sampling technique used. Microbial colonization, retention and community composition on different dry surfaces are very complex and rely on numerous physicochemical and biological factors. This study is devoted to analyze and review the (a) physical phenomena and intermolecular forces relevant for microbiological surface sampling; (b) challenges and problems faced by existing sampling methods for viable microorganisms and (c) current directions of engineering and research aimed at improvement of quality and efficiency of microbiological surface sampling. KW - Sampling methods KW - Surface microorganisms KW - Dry surfaces KW - Microbial adhesion KW - Swabbing Y1 - 2018 SN - 978-981-10-7904-7 U6 - https://doi.org/10.1007/978-981-10-7904-7_19 SP - 421 EP - 456 PB - Springer CY - Singapore ER - TY - JOUR A1 - de Blois, Eric A1 - de Zanger, Rory M. S. A1 - Paulßen, Elisabeth A1 - Sze Chan, Ho A1 - Breeman, Wouter A. P. T1 - Semi-automated system for concentrating 68Ga-eluate to obtain high molar and volume concentration of 68Ga-Radiopharmaca for preclinical applications JF - Nuclear Medicine and Biology N2 - 68Ga-radiopharmaceuticals are common in the field of Nuclear Medicine to visualize receptor-mediated processes. In contrast to straightforward labeling procedures for clinical applications, preclinical in vitro and in vivo applications are hampered for reasons like e.g. volume restriction, activity concentration, molar activity and osmolality. Therefore, we developed a semiautomatic system specifically to overcome these problems. A difficulty appeared unexpectedly, as intrinsic trace metals derived from eluate (Zn, Fe and Cu) are concentrated as well in amounts that influence radiochemical yield and thus lower molar activity. Y1 - 2018 U6 - https://doi.org/10.1016/j.nucmedbio.2018.06.006 VL - 64-65 SP - 16 EP - 21 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Dantism, Shahriar A1 - Röhlen, Desiree A1 - Wagner, Torsten A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Optimization of Cell-Based Multi-Chamber LAPS Measurements Utilizing FPGA-Controlled Laser-Diode Modules JF - physica status solidi a : applications and materials sciences N2 - A light-addressable potentiometric sensor (LAPS) is a field-effect-based potentiometric device, which detects concentration changes of an analyte solution on the sensor surface in a spatially resolved way. It uses a light source to generate electron–hole pairs inside the semiconductor, which are separated in the depletion region due to an applied bias voltage across the sensor structure and hence, a surface-potential-dependent photocurrent can be read out. However, depending on the beam angle of the light source, scattering effects can occur, which influence the recorded signal in LAPS-based differential measurements. To solve this problem, a novel illumination unit based on a field programmable gate array (FPGA) consisting of 16 small-sized tunable infrared laser-diode modules (LDMs) is developed. Due to the improved focus of the LDMs with a beam angle of only 2 mrad, undesirable scattering effects are minimized. Escherichia coli (E. coli) K12 bacteria are used as a test microorganism to study the extracellular acidification on the sensor surface. Furthermore, a salt bridge chamber is built up and integrated with the LAPS system enabling multi-chamber differential measurements with a single Ag/AgCl reference electrode. Y1 - 2018 U6 - https://doi.org/10.1002/pssa.201800058 SN - 1862-6319 VL - 215 IS - 15 SP - Article number 1800058 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Czarnecki, Christian A1 - Auth, Gunnar ED - Barton, Thomas ED - Müller, Christian ED - Seel, Christian T1 - Prozessdigitalisierung durch Robotic 7 Process Automation T2 - Digitalisierung in Unternehmen: von den theoretischen Ansätzen zur praktischen Umsetzung N2 - Im Rahmen der digitalen Transformation werden innovative Technologiekonzepte, wie z. B. das Internet der Dinge und Cloud Computing als Treiber für weitreichende Veränderungen von Organisationen und Geschäftsmodellen angesehen. In diesem Kontext ist Robotic Process Automation (RPA) ein neuartiger Ansatz zur Prozessautomatisierung, bei dem manuelle Tätigkeiten durch sogenannte Softwareroboter erlernt und automatisiert ausgeführt werden. Dabei emulieren Softwareroboter die Eingaben auf der bestehenden Präsentationsschicht, so dass keine Änderungen an vorhandenen Anwendungssystemen notwendig sind. Die innovative Idee ist die Transformation der bestehenden Prozessausführung von manuell zu digital, was RPA von traditionellen Ansätzen des Business Process Managements (BPM) unterscheidet, bei denen z. B. prozessgetriebene Anpassungen auf Ebene der Geschäftslogik notwendig sind. Am Markt werden bereits unterschiedliche RPA-Lösungen als Softwareprodukte angeboten. Gerade bei operativen Prozessen mit sich wiederholenden Verarbeitungsschritten in unterschiedlichen Anwendungssystemen sind gute Ergebnisse durch RPA dokumentiert, wie z. B. die Automatisierung von 35 % der Backoffice-Prozesse bei Telefonica. Durch den vergleichsweise niedrigen Implementierungsaufwand verbunden mit einem hohen Automatisierungspotenzial ist in der Praxis (z. B. Banken, Telekommunikation, Energieversorgung) ein hohes Interesse an RPA vorhanden. Der Beitrag diskutiert RPA als innovativen Ansatz zur Prozessdigitalisierung und gibt konkrete Handlungsempfehlungen für die Praxis. Dazu wird zwischen modellgetriebenen und selbstlernenden Ansätzen unterschieden. Anhand von generellen Architekturen von RPA-Systemen werden Anwendungsszenarien sowie deren Automatisierungspotenziale, aber auch Einschränkungen, diskutiert. Es folgt ein strukturierter Marktüberblick ausgewählter RPA-Produkte. Anhand von drei konkreten Anwendungsbeispielen wird die Nutzung von RPA in der Praxis verdeutlicht. KW - Prozessautomatisierung KW - Geschäftsprozessmanagement KW - Softwareroboter KW - Digitale Transformation KW - Projektbeispiele Y1 - 2018 SN - 9783658227739 SN - 9783658227722 U6 - https://doi.org/10.1007/978-3-658-22773-9_7 SP - 113 EP - 131 PB - Springer CY - Wiesbaden ER -