TY  - JOUR
A1  - Bäcker, Matthias
A1  - Rakowski, D.
A1  - Poghossian, Arshak
A1  - Biselli, Manfred
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis
JF  - Journal of Biotechnology
N2  - A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
Y1  - 2013
U6  - https://doi.org/10.1016/j.jbiotec.2012.03.014
SN  - 0168-1656
VL  - 163
IS  - 4
SP  - 371
EP  - 376
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Beverungen, Daniel
A1  - Eggert, Mathias
A1  - Voigt, Matthias
A1  - Rosemann, Michael
T1  - Augmenting Analytical CRM Strategies with Social BI
JF  - International Journal of Business Intelligence Research (IJBIR)
Y1  - 2013
U6  - https://doi.org/10.4018/ijbir.2013070103
SN  - 1947-3591
VL  - 4
IS  - 3
SP  - 32
EP  - 49
PB  - IGI Global
CY  - Hershey
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hecker, Michael
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production
JF  - Microbial Cell Factories
Y1  - 2013
U6  - https://doi.org/10.1186/1475-2859-12-120
SN  - 1475-2859
VL  - 12
SP  - 120
PB  - Biomed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Menzel, S.
A1  - Bäcker, Matthias
A1  - Leinhos, Marcel
A1  - Poghossian, Arshak
A1  - Wagner, P.
A1  - Schöning, Michael Josef
T1  - Degradation of thin poly(lactic acid) films: characterization by capacitance-voltage, atomic force microscopy, scanning electron microscopy and contact-angle measurements
JF  - Electrochimica Acta
Y1  - 2013
SN  - 1873-3859 (E-Journal); 0013-4686 (Print)
VL  - Vol. 113
SP  - 779
EP  - 784
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Micili, Serap C.
A1  - Valter, Markus
A1  - Oflaz, Hakan
A1  - Ozogul, Candan
A1  - Linder, Peter
A1  - Föckler, Nicole
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - Optical coherence tomography : a potential tool to predict premature rupture of fetal membranes
JF  - Proceedings of the Institution of Mechanical Engineers. Part H : Journal of engineering in medicine
Y1  - 2013
SN  - 0046-2039 (Print) ; 2041-3033 (E-Journal)
VL  - Vol. 227
IS  - No. 4
SP  - 393
EP  - 401
PB  - Sage
CY  - London
ER  - 
TY  - CHAP
A1  - Becker, Jörg
A1  - Eggert, Mathias
A1  - Fleischer, Stefan
A1  - Knackstedt, Ralf
T1  - How to Teach Regulatory Compliant Data Warehouse Engineering?
T2  - Proceedings of the Nineteenth Americas Conference on Information Systems, Chicago, Illinois, August 15-17, 2013.
Y1  - 2013
N1  - AMCIS 2013 Proceedings
ER  - 
TY  - CHAP
A1  - Gogolin, C.
A1  - Carvalho, R.
A1  - Bung, Daniel Bernhard
A1  - Matos, J.
T1  - Experimental and numerical investigation of steady and unsteady flows in roughened channels with cross beams
T2  - 35th IAHR world congress : 8.-13.9.2013, Chengdu, China
Y1  - 2013
SP  - Artikelkennnummer: A10837C
PB  - Tsinghua Univ. Press
CY  - Beijing
ER  - 
TY  - CHAP
A1  - Oertel, Mario
A1  - Bung, Daniel Bernhard
T1  - Comparison of 2D dam-break waves with VOF and SPH method
T2  - Proceedings of the 35th IAHR world congress : 8.-13.9.2013, Chengdu, China
Y1  - 2013
SP  - Artikelkennnummer: A11113
PB  - Tsinghua Univ. Press
CY  - Beijing
ER  - 
TY  - CHAP
A1  - Bung, Daniel Bernhard
T1  - Air-water surface roughness in self-aerated stepped spillway flows
T2  - 35th IAHR world congress : 8.-13.9.2013, Chengdu, China
Y1  - 2013
SP  - Artikelkennnummer: A11045
PB  - Tsinghua Univ. Press
CY  - Beijing
ER  - 
TY  - CHAP
A1  - Becker, Jörg
A1  - Eggert, Mathias
A1  - Bruning, Dennis
A1  - Saat, Jan
T1  - Applicability of Business Process Model Analysis Approaches – A Case Study in Financial Services Consulting
T2  - Wirtschaftsinformatik Proceedings 2013
Y1  - 2013
N1  - 11th International Conference on Wirtschaftsinformatik,
27th February – 01st March 2013, Leipzig, Germany
SP  - 1181
EP  - 1195
ER  -