TY - JOUR A1 - Christiaens, Pieter A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Wagner, Torsten A1 - Bijnens, Nathalie A1 - Williams, Oliver A. A1 - Daenen, Michael A1 - Haenen, Ken A1 - Schöning, Michael Josef A1 - Wagner, Patrick T1 - Nanocrystalline diamond-based field-effect capacitive pH sensor JF - Transducers '07 Eurosensors XXI : digest of technical papers ; the14th International Conference on Solid-State Sensors, Actuators and Microsystems, June 10-14, 2007, Lyon, France / Gilles Delapierre (Ed.) Y1 - 2007 SN - 1-4244-0841-5 N1 - Eurosensors 21, 2007, Lyon ; International Conference on Solid-State Sensors, Actuators and Microsystems 14, 2007, Lyon SP - 1891 EP - 1894 PB - IEEE CY - Piscataway ER - TY - JOUR A1 - Poghossian, Arshak A1 - Abouzar, Maryam H. A1 - Razavi, Arash A1 - Bäcker, Matthias A1 - Bijnens, Nathalie A1 - Williams, Oliver A. A1 - Haenen, Ken A1 - Moritz, Werner A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Nanocrystalline-diamond thin films with high pH and penicillin sensitivity prepared on a capacitive Si–SiO2 structure JF - Electrochimica Acta. 54 (2009), H. 25 Y1 - 2009 SN - 0013-4686 SP - 5981 EP - 5985 ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Razavi, Arash A1 - Williams, Oliver A. A1 - Bijnens, Nathalie A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Characterisation of capacitive field-effect sensors with a nanocrystalline-diamond film as transducer material for multi-parameter sensing JF - Biosensors and Bioelectronics. 24 (2009), H. 5 Y1 - 2009 SN - 0956-5663 N1 - Selected Papers from the Tenth World Congress on Biosensors Shangai, China, May 14-16, 2008 ; Zeitschrift früher u.d.T. : Biosensors SP - 1298 EP - 1304 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schuba, Marko A1 - Reichl, Peter T1 - How to Place Connectionless Servers in ATM Networks / Schuba, Marko ; Reichl, Peter JF - Proc. of Fourth IFIP Workshop on Performance Modelling and Evaluation of ATM Networks Y1 - 1996 N1 - Fourth IFIP Workshop on Performance Modelling and Evaluation of ATM Networks, Ilkley, West Yorkshire, U.K., July 1996 SP - 09/1 EP - 09/10 ER - TY - JOUR A1 - Ribitsch, Doris A1 - Karl, Wolfgang A1 - Birner-Gruenberger, Ruth A1 - Gruber, Karl A1 - Eiteljoerg, Inge A1 - Remler, Peter A1 - Wieland, Susanne A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, Helmut T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - https://doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Ribitsch, Doris A1 - Heumann, Sonja A1 - Trotscha, Eva A1 - Herrero Acero, Enrique A1 - Greimel, Katrin A1 - Leber, Regina A1 - Birner-Gruenberger, Ruth A1 - Deller, Sigrid A1 - Eiteljoerg, Inge A1 - Remler, Peter A1 - Weber, Thomas A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Donelli, Ilaria A1 - Freddi, Giuliano A1 - Schwab, Helmut A1 - Guebitz, Georg M. T1 - Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis JF - Biotechnology progress Y1 - 2011 SN - 1520-6033 (E-Journal); 8756-7938 (Print) VL - Vol. 27 IS - Iss. 4 SP - 951 EP - 960 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Schaefer, Thomas A1 - Höfken, Hans-Wilhelm A1 - Schuba, Marko T1 - Windows Phone 7 from a Digital Forensics’ Perspective Y1 - 2011 U6 - https://doi.org/10.1007/978-3-642-35515-8_6 N1 - ICDF2C <3, 2011, Dublin> PB - Springer CY - Berlin ER - TY - JOUR A1 - Schuba, Marko A1 - Reichl, Peter A1 - Kesdogan, Dogan A1 - Junghärtchen, Klaus T1 - Simulative Performance Evaluation of the Temporary Pseudonym Method for Protecting Location Information in GSM Networks / Reichl, P. ; Kesdogan, D. ; Junghärtchen, K. ; Schuba, M. JF - Computer Performance Evaluation - Modelling Techniques and Tools / Puigjaner, Ramon (eds.) Y1 - 1998 SN - 3-540-64949-2 N1 - Lecture notes in computer science ; 1469 SP - 105 EP - 116 PB - Springer CY - Berlin ER - TY - JOUR A1 - Schuba, Marko A1 - Haverkort, Boudewijn R. A1 - Schneider, Gaby T1 - Performance evaluation of multicast communication in packet-switched networks / Schuba, Marko ; Haverkort, Boudewijn R. ; Schneider, Gaby JF - Performance Evaluation. 39 (2000), H. 1-4 Y1 - 2000 SN - 0166-5316 U6 - https://doi.org/10.1016/S0166-5316(99)00058-9 SP - 61 EP - 80 ER - TY - JOUR A1 - Schuba, Marko A1 - Hermanns, Oliver T1 - Performance Investigations of the IP Multicast Architecture / Hermanns, Oliver ; Schuba, Marko JF - Performance of the IP Multicast Achitecture . Proceedings JENC 6. Proceedings of the 6th Joint European Networking Conference, Tel Aviv Y1 - 1995 N1 - Reprinted in Computer Networks and ISDN Systems 28 pp 429-439, 1996 SP - 121-1 EP - 121-8 ER -