TY  - JOUR
A1  - Seibler, Jost
A1  - Schübeler, Dirk
A1  - Fiering, Steven
A1  - Groudine, Mark
A1  - Bode, Jürgen
T1  - DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs
JF  - Biochemistry
Y1  - 1998
U6  - https://doi.org/10.1021/bi980288t
SN  - 1520-4995
VL  - 37
IS  - 18
SP  - 6229
EP  - 6234
ER  - 
TY  - CHAP
A1  - Seibler, Jost
A1  - Schwenk, Frieder
T1  - Transgenic RNAi Applications in the Mouse
T2  - Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition
Y1  - 2010
SN  - 978-0-12-384880-2
N1  - Methods in Enzymology : Vol. 477
SP  - 367
EP  - 386
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Küter-Luks, Birgit
A1  - Kern, Heidrun
A1  - Streu, Sandra
A1  - Plum, Leona
A1  - Maurer, Jan
A1  - Kühn, Ralf
A1  - Brüning, Jens C.
A1  - Schwenk, Frieder
T1  - Single copy shRNA configuration for ubiquitous gene knockdown in mice
JF  - Nucleic Acids Research
Y1  - 2005
U6  - https://doi.org/10.1093/nar/gni065
SN  - 1362-4962
VL  - 33
IS  - 7
SP  - e67
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Kleinridders, Andre
A1  - Küter-Luks, Birgit
A1  - Niehaves, Sandra
A1  - Brüning, Jens C.
A1  - Schwenk, Frieder
T1  - Reversible gene knockdown in mice using a tight, inducible shRNA expression system
JF  - Nucleic Acids Research
Y1  - 2007
U6  - https://doi.org/10.1093/nar/gkm122
SN  - 1362-4962
VL  - 35
IS  - 7
SP  - e54
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Bode, Jürgen
T1  - Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay
JF  - Biochemistry
Y1  - 1997
SN  - 1520-4995
VL  - 36
IS  - 7
SP  - 1740
EP  - 1747
ER  - 
TY  - JOUR
A1  - Schwab, Lukas
A1  - Hojdis, Nils
A1  - Lacayo, Jorge
A1  - Wilhelm, Manfred
T1  - Fourier-Transform Rheology of Unvulcanized, Carbon Black Filled Styrene Butadiene Rubber
JF  - Macromolecular Materials and Engineering
N2  - Rubber materials filled with reinforcing fillers display nonlinear rheological behavior at small strain amplitudes below γ0 < 0.1. Nevertheless, rheological data are analyzed mostly in terms of linear parameters, such as shear moduli (G′, G″), which loose their physical meaning in the nonlinear regime. In this work styrene butadiene rubber filled with carbon black (CB) under large amplitude oscillatory shear (LAOS) is analyzed in terms of the nonlinear parameter I3/1. Three different CB grades are used and the filler load is varied between 0 and 70 phr. It is found that I3/1(φ) is most sensitive to changes of the total accessible filler surface area at low strain amplitudes (γ0 = 0.32). The addition of up to 70 phr CB leads to an increase of I3/1(φ) by a factor of more than ten. The influence of the measurement temperature on I3/1 is pronounced for CB levels above the percolation threshold.
Y1  - 2016
U6  - https://doi.org/10.1002/mame.201500356
SN  - 1439-2054
VL  - 301
IS  - 4
SP  - 457
EP  - 468
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schroeter, Rebecca
A1  - Hoffmann, Tamara
A1  - Voigt, Birgit
A1  - Meyer, Hanna
A1  - Bleisteiner, Monika
A1  - Muntel, Jan
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Becher, Dörte
A1  - Lalk, Michael
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Putzer, Harald
A1  - Hecker, Michael
A1  - Schweder, Thomas
A1  - Bremer, Erhard
T1  - Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges
JF  - PLoS ONE
N2  - The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress.
Y1  - 2014
U6  - https://doi.org/10.1371/journal.pone.0080956
SN  - 1932-6203
VL  - 8
IS  - 11
PB  - PLOS
CY  - San Francisco
ER  - 
TY  - JOUR
A1  - Schnitzler, Thomas
T1  - Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)
JF  - Application notes / Sartorius stedim biotech
Y1  - 2009
SP  - 1
EP  - 4
ER  - 
TY  - CHAP
A1  - Schnabel, Eberhard
A1  - Berndt, Heinz
ED  - Nesvadba, H.
T1  - Zur selektive Abspaltbarkeit der t-Butyloxycarbonylgruppe
T2  - Peptides 1971 : proceedings of the Eleventh European Peptide Symposium, Vienna, Austria, April 1971
Y1  - 1973
SN  - 0-7204-4120-X
SP  - 69
EP  - 70
PB  - North-Holland Publ. [u.a.]
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Schmitz, M.
A1  - Hirsch, E.
A1  - Bongaerts, Johannes
A1  - Takors, Ralf
T1  - Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli
JF  - Biotechnology progress
Y1  - 2002
SN  - 1520-6033 (E-Journal); 8756-7938 (Print)
VL  - Vol. 18
IS  - Iss. 5
SP  - 935
EP  - 941
ER  -