TY  - JOUR
A1  - Aggarwal, Prerna
A1  - Singh, Virendra
A1  - Singh, Arjun
A1  - Scherer, Ulrich W.
A1  - Singh, Tejvir
A1  - Singla, Madan L.
A1  - Srivastava, Alok
T1  - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate)
JF  - Radiation Physics and Chemistry
Y1  - 2011
SN  - 0969-806X
N1  - In Press, Accepted Manuscript
SP  - 1
EP  - 28
PB  - Pergamon Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Aggarwal, P.
A1  - Dhiman, S.
A1  - Kumar, G.
A1  - Scherer, Ulrich W.
A1  - Singla, M. L.
A1  - Srivastava, A.
T1  - Optical study of poly(ethyleneterephthalate) modified by different ionizing radiation dose
JF  - Indian Journal of Pure and Applied Physics
N2  - Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions.
Y1  - 2012
SN  - 0019-5596
VL  - 50
IS  - 2
SP  - 129
EP  - 132
ER  - 
TY  - JOUR
A1  - Abulnaga, El-Hussiny
A1  - Pinkenburg, Olaf
A1  - Schiffels, Johannes
A1  - E-Refai, Ahmed
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Effect of an Oxygen-Tolerant Bifurcating Butyryl Coenzyme A Dehydrogenase/Electron-Transferring Flavoprotein Complex from Clostridium difficile on Butyrate Production in Escherichia coli
JF  - Journal of bacteriology
Y1  - 2013
SN  - 1098-5530 [E-Journal]
SN  - 0021-9193 [Print]
VL  - 195
IS  - 16
SP  - 3704
EP  - 3713
ER  - 
TY  - JOUR
A1  - Aboulnaga, E. H.
A1  - Pinkenburg, O.
A1  - Schiffels, Johannes
A1  - El-Refai, A.
A1  - Buckel, W.
A1  - Selmer, Thorsten
T1  - Butyrate production in Escherichia coli: Exploitation of an oxygen tolerant bifurcating butyryl-CoA dehydrogenase/electron transferring flavoprotein complex from Clostridium difficile
JF  - Journal of bacteriology. June 14, 2013
Y1  - 2013
SN  - 1098-5530 (E-Journal) ; 0021-9193 (Print)
SP  - Epub ahead of print
ER  - 
TY  - JOUR
A1  - Aboulnaga, E. A.
A1  - Zou, H.
A1  - Selmer, Thorsten
A1  - Xian, M.
T1  - Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16
JF  - Journal of Biotechnology
N2  - Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.
Y1  - 2018
U6  - http://dx.doi.org/10.1016/j.jbiotec.2018.03.007
SN  - 0168-1656
VL  - 274
SP  - 15
EP  - 27
PB  - Elsevier
CY  - Amsterdam
ER  -