TY - CHAP A1 - Siegert, Petra A1 - Pohl, Martina A1 - Kneen, Malea M. A1 - Pogozheva, Irina D. A1 - Kenyon, George L. A1 - McLeish, Michael J. T1 - Exploring the substrate specificity of benzoylformate decarboxylase, pyruvate decarboxylase, and benzaldehyde lyase T2 - Thiamine : catalytic mechanisms in normal and disease states / ed. by Frank Jordan ... Y1 - 2004 SN - 0-8247-4062-9 SP - 275 EP - 290 PB - Dekker CY - New York, NY ER - TY - JOUR A1 - Dünkelmann, Pascal A1 - Kolter-Jung, Doris A1 - Nitsche, Adam A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Lingen, Bettina A1 - Baumann, Martin A1 - Pohl, Martina A1 - Müller, Michael T1 - Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation JF - Journal of the American Chemical Society Y1 - 2002 SN - 1520-5126 (E-Journal); 0002-7863 (Print) VL - Vol. 124 SP - 12084 EP - 12085 ER - TY - JOUR A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Pohl, Martina A1 - Müller, Michael T1 - ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation JF - Cheminform Y1 - 2001 SN - 1522-2667 (E-Journal); 0931-7597 (Print) VL - Vol. 32 IS - Iss. 4 SP - Publ. online ER - TY - JOUR A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Siegert, Petra A1 - Pohl, Martina A1 - Müller, Michael T1 - Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C−C Bond Formation JF - European journal of organic chemistry Y1 - 2000 SN - 0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print) VL - Vol. 2000 IS - Iss. 11 SP - 2161 EP - 2170 ER - TY - CHAP A1 - Siegert, Petra A1 - Iding, Hans A1 - Baumann, Martin A1 - McLeish, Michael J. A1 - Kenyon, George L. A1 - Pohl, Martina T1 - Broadening of the substrate spectra of two ThDP-dependent decarboxylases using site-directed-mutagenesis T2 - Proceedings of the 4th International Congress on Biochemical Engineering : 17 and 18 February 2000, Stuttgart Y1 - 2000 SN - 3-8167-5570-4 SP - 38 EP - 42 ER - TY - JOUR A1 - Iding, Hans A1 - Dünnwald, Thomas A1 - Greiner, Lasse A1 - Liese, Andreas A1 - Müller, Michael A1 - Siegert, Petra A1 - Grötzinger, Joachim A1 - Demir, Ayhan S. A1 - Pohl, Martina T1 - Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones JF - Chemistry - a European journal Y1 - 2000 SN - 1521-3765 (E-Journal); 0947-6539 (Print) VL - Vol. 6 IS - Iss. 8 SP - 1483 EP - 1495 ER - TY - JOUR A1 - Iding, Hans A1 - Siegert, Petra A1 - Mesch, K. A1 - Pohl, Martina T1 - Application of α-keto acid decarboxylases in biotransformations JF - Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology Y1 - 1998 SN - 1879-2588 (E-Journal); 0167-4838 (Print) VL - Vol. 1385 IS - Iss. 2 SP - 307 EP - 322 ER - TY - JOUR A1 - Pohl, Martina A1 - Siegert, Petra A1 - Mesch, K. A1 - Bruhn, H. A1 - Grötzinger, Joachim T1 - Active site mutants of pyruvate decarboxylase from Zymomonas mobilis : a site-directed mutagenesis study of L112, I472, I476, E473 and N482 JF - European journal of biochemistry Y1 - 1998 SN - 1432-1033 (E-Journal); 1742-4658 (E-Journal); 0014-2956 (Print); 1742-464X (Print) VL - Vol. 257 IS - Iss. 3 SP - 538 EP - 546 ER - TY - JOUR A1 - Ratke, Lorenz A1 - Milow, Barbara A1 - Lisinski, Susanne A1 - Hoepfner, Sandra T1 - On an effect of fine ceramic particles on the structure of aerogels JF - Microgravity science and technology Y1 - 2014 U6 - http://dx.doi.org/10.1007/s12217-014-9380-2 SN - 0938-0108 ; 1875-0494 VL - 26 SP - 103 EP - 110 PB - Springer Nature CY - Heidelberg ER - TY - JOUR A1 - Handtke, Stefan A1 - Volland, Sonja A1 - Methling, Karen A1 - Albrecht, Dirk A1 - Becher, Dörte A1 - Nehls, Jenny A1 - Bongaerts, Johannes A1 - Maurer, Karl-Heinz A1 - Lalk, Michael A1 - Liesegang, Heiko A1 - Voigt, Birgit A1 - Daniel, Rolf A1 - Hecker, Michael T1 - Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach JF - Journal of Biotechnology N2 - Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC–MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed. Y1 - 2014 U6 - http://dx.doi.org/10.1016/j.jbiotec.2014.08.028 SN - 1873-4863 (E-Journal); 0168-1656 (Print) IS - 192(A) SP - 204 EP - 214 PB - Elsevier CY - Amsterdam ER -