TY - JOUR A1 - Ribitsch, Doris A1 - Karl, Wolfgang A1 - Birner-Gruenberger, Ruth A1 - Gruber, Karl A1 - Eiteljoerg, Inge A1 - Remler, Peter A1 - Wieland, Susanne A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, Helmut T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - https://doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Götten, Falk A1 - Finger, Felix A1 - Braun, Carsten A1 - Havermann, Marc A1 - Bil, Cees A1 - Gomez, Francisco T1 - Empirical Correlations for Geometry Build-Up of Fixed Wing Unmanned Air Vehicles T2 - APISAT 2018: The Proceedings of the 2018 Asia-Pacific International Symposium on Aerospace Technology (APISAT 2018) N2 - The results of a statistical investigation of 42 fixed-wing, small to medium sized (20 kg−1000 kg) reconnaissance unmanned air vehicles (UAVs) are presented. Regression analyses are used to identify correlations of the most relevant geometry dimensions with the UAV’s maximum take-off mass. The findings allow an empirical based geometry-build up for a complete unmanned aircraft by referring to its take-off mass only. This provides a bridge between very early design stages (initial sizing) and the later determination of shapes and dimensions. The correlations might be integrated into a UAV sizing environment and allow designers to implement more sophisticated drag and weight estimation methods in this process. Additional information on correlation factors for a rough drag estimation methodology indicate how this technique can significantly enhance the accuracy of early design iterations. KW - Unmanned Air Vehicle KW - Geometry KW - Correlations KW - Statistics KW - Drag Y1 - 2019 SN - 978-981-13-3305-7 U6 - https://doi.org/10.1007/978-981-13-3305-7_109 N1 - APISAT 2018 - Asia-Pacific International Symposium on Aerospace Technology. 16-18 October 2018. Chengdu, China. Lecture Notes in Electrical Engineering (LNEE, volume 459) SP - 1365 EP - 1381 PB - Springer CY - Singapore ER - TY - JOUR A1 - Ribitsch, Doris A1 - Heumann, Sonja A1 - Trotscha, Eva A1 - Herrero Acero, Enrique A1 - Greimel, Katrin A1 - Leber, Regina A1 - Birner-Gruenberger, Ruth A1 - Deller, Sigrid A1 - Eiteljoerg, Inge A1 - Remler, Peter A1 - Weber, Thomas A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Donelli, Ilaria A1 - Freddi, Giuliano A1 - Schwab, Helmut A1 - Guebitz, Georg M. T1 - Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis JF - Biotechnology progress Y1 - 2011 SN - 1520-6033 (E-Journal); 8756-7938 (Print) VL - Vol. 27 IS - Iss. 4 SP - 951 EP - 960 PB - Wiley CY - Hoboken ER - TY - CHAP A1 - Uibel, Thomas A1 - Blaß, Hans Joachim T1 - Joints with Dowel Type Fasteners in CLT structures T2 - Focus solid timber solutions : European Conference on Cross Laminated Timber (CLT); May 21-22, 2013, Graz University of Technology, Austria Y1 - 2013 SN - 978-1-85790-181-8 ; 1-85790-181-9 SP - 119 EP - 134 ER - TY - JOUR A1 - Schaefer, Thomas A1 - Höfken, Hans-Wilhelm A1 - Schuba, Marko T1 - Windows Phone 7 from a Digital Forensics’ Perspective Y1 - 2011 U6 - https://doi.org/10.1007/978-3-642-35515-8_6 N1 - ICDF2C <3, 2011, Dublin> PB - Springer CY - Berlin ER - TY - CHAP A1 - Broenner, Simon A1 - Höfken, Hans-Wilhelm A1 - Schuba, Marko T1 - Streamlining extraction and analysis of android RAM images T2 - Proceedings of the 2nd International Conference on Information Systems Security and Privacy - ICISSP N2 - The Android operating system powers the majority of the world’s mobile devices and has been becoming increasingly important in day-to-day digital forensics. Therefore, technicians and analysts are in need of reliable methods for extracting and analyzing memory images from live Android systems. This paper takes different existing, extraction methods and derives a universal, reproducible, reliably documented method for both extraction and analysis. In addition the VOLIX II front-end for the Volatility Framework is extended with additional functionality to make the analysis of Android memory images easier for technically non-adept users. Y1 - 2016 SN - 978-989-758-167-0 U6 - https://doi.org/10.5220/0005652802550264 SP - 255 EP - 264 PB - SciTePress CY - Setúbal ER - TY - CHAP A1 - Schütz, P. A1 - Breuer, M. A1 - Höfken, Hans-Wilhelm A1 - Schuba, Marko T1 - Malware proof on mobile phone exhibits based on GSM/GPRS traces T2 - The Second International Conference on Cyber Security, Cyber Peacefare and Digital Forensic (CyberSec 2013) : 04.03. - 06.03.2013, Kuala Lumpur, Malaysia Y1 - 2013 SN - 978-0-9853483-7-3 SP - 89 EP - 96 PB - The Society of Digital Information and Wireless Communication ER - TY - JOUR A1 - Schuba, Marko A1 - Reichl, Peter A1 - Kesdogan, Dogan A1 - Junghärtchen, Klaus T1 - Simulative Performance Evaluation of the Temporary Pseudonym Method for Protecting Location Information in GSM Networks / Reichl, P. ; Kesdogan, D. ; Junghärtchen, K. ; Schuba, M. JF - Computer Performance Evaluation - Modelling Techniques and Tools / Puigjaner, Ramon (eds.) Y1 - 1998 SN - 3-540-64949-2 N1 - Lecture notes in computer science ; 1469 SP - 105 EP - 116 PB - Springer CY - Berlin ER - TY - JOUR A1 - Schuba, Marko A1 - Haverkort, Boudewijn R. A1 - Schneider, Gaby T1 - Performance evaluation of multicast communication in packet-switched networks / Schuba, Marko ; Haverkort, Boudewijn R. ; Schneider, Gaby JF - Performance Evaluation. 39 (2000), H. 1-4 Y1 - 2000 SN - 0166-5316 U6 - https://doi.org/10.1016/S0166-5316(99)00058-9 SP - 61 EP - 80 ER - TY - JOUR A1 - Schuba, Marko A1 - Hermanns, Oliver T1 - Performance Investigations of the IP Multicast Architecture / Hermanns, Oliver ; Schuba, Marko JF - Performance of the IP Multicast Achitecture . Proceedings JENC 6. Proceedings of the 6th Joint European Networking Conference, Tel Aviv Y1 - 1995 N1 - Reprinted in Computer Networks and ISDN Systems 28 pp 429-439, 1996 SP - 121-1 EP - 121-8 ER -