TY - JOUR A1 - Uysal, Karya A1 - Firat, Ipek Serat A1 - Creutz, Till A1 - Aydin, Inci Cansu A1 - Artmann, Gerhard A1 - Teusch, Nicole A1 - Temiz Artmann, Aysegül T1 - A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes JF - membranes N2 - Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here. Y1 - 2022 U6 - https://doi.org/10.3390/membranes13010022 N1 - This article belongs to the Special Issue "Latest Scientific Discoveries in Polymer Membranes" VL - 2023 IS - 13(1) PB - MDPI CY - Basel ER - TY - GEN A1 - Dieringer, Matthias A. A1 - Renz, Wolfgang A1 - Lindel, Tomasz Dawid A1 - Seifert, Frank A1 - Frauenrath, Tobias A1 - Waiczies, Helmar A1 - von Knobelsdorff-Brenkhoff, Florian A1 - Santoro, Davide A1 - Hoffmann, Werner A1 - Ittermann, Bernd A1 - Schulz-Menger, Jeanette A1 - Niendorf, Thoralf T1 - 4CH TX/RX Surface Coil for 7T: Design, Optimization and Application for Cardiac Function Imaging T2 - 2010 ISMRM-ESMRMB joint annual meeting N2 - Practical impediments of ultra high field cardiovascular MR (CVMR) can be catalogued in exacerbated magnetic field and radio frequency (RF) inhomogeneities, susceptibility and off-resonance effects, conductive and dielectric effects in tissue, and RF power deposition constraints, which all bear the potential to spoil the benefit of CVMR at 7T. Therefore, a four element cardiac transceive surface coil array was developed. Cardiac imaging provided clinically acceptable signal homogeneity with an excellent blood myocardium contrast. Subtle anatomic structures, such as pericardium, mitral and tricuspid valves and their apparatus, papillary muscles, and trabecles were accurately delineated. Y1 - 2010 SN - 1545-4428 N1 - ISMRM-ESMRMB joint annual meeting, 1 - 7 May 2010, Stockholm, Sweden ER - TY - CHAP A1 - Schmitz, Annika A1 - Apandi, Shah Eiman Amzar Shah A1 - Spillner, Jan A1 - Hima, Flutura A1 - Behbahani, Mehdi ED - Digel, Ilya ED - Staat, Manfred ED - Trzewik, Jürgen ED - Sielemann, Stefanie ED - Erni, Daniel ED - Zylka, Waldemar T1 - Effect of different cannula positions in the pulmonary artery on blood flow and gas exchange using computational fluid dynamics analysis T2 - YRA MedTech Symposium (2024) N2 - Pulmonary arterial cannulation is a common and effective method for percutaneous mechanical circulatory support for concurrent right heart and respiratory failure [1]. However, limited data exists to what effect the positioning of the cannula has on the oxygen perfusion throughout the pulmonary artery (PA). This study aims to evaluate, using computational fluid dynamics (CFD), the effect of different cannula positions in the PA with respect to the oxygenation of the different branching vessels in order for an optimal cannula position to be determined. The four chosen different positions (see Fig. 1) of the cannulas are, in the lower part of the main pulmonary artery (MPA), in the MPA at the junction between the right pulmonary artery (RPA) and the left pulmonary artery (LPA), in the RPA at the first branch of the RPA and in the LPA at the first branch of the LPA. Y1 - 2024 SN - 978-3-940402-65-3 U6 - https://doi.org/10.17185/duepublico/81475 N1 - 4th YRA MedTech Symposium, February 1, 2024. FH Aachen, Campus Jülich SP - 29 EP - 30 PB - Universität Duisburg-Essen CY - Duisburg ER - TY - CHAP A1 - Engelmann, Ulrich M. A1 - Baumann, Martin ED - Thomann, Hermann ED - Träger, Thomas T1 - Moderationsexpertise für QMBs – das Mindset T2 - Qualitätsmanagement in Dienstleistungsunternehmen N2 - Teamsitzungen, Arbeitsgruppentreffen, Kickoffs und Meetings – sie alle werden mit dem Ziel durchgeführt, innerhalb einer vorgegebenen Zeitspanne ein gemeinsames Arbeitsziel zu erreichen. Damit die Zielerreichung auch bei komplexeren Arbeitsaufträgen nicht vom Zufall abhängt, empfiehlt es sich, die Leitung des Ablaufs einem Moderator zu übertragen. In diesem Beitrag einer mehrteiligen Serie wird beschrieben, über welches Mindset der Moderator verfügen sollte, welche grundsätzlichen Methoden hilfreich sind und was bei der Onlinemoderation im Besonderen zu beachten ist. Y1 - 2023 SN - 978-3-8249-0473-0 SP - Kapitel 08630 PB - TÜV-Verlag CY - Köln ER - TY - CHAP A1 - Kleefeld, Andreas ED - Constanda, Christian T1 - Numerical calculation of interior transmission eigenvalues with mixed boundary conditions T2 - Computational and Analytic Methods in Science and Engineering N2 - Interior transmission eigenvalue problems for the Helmholtz equation play an important role in inverse wave scattering. Some distribution properties of those eigenvalues in the complex plane are reviewed. Further, a new scattering model for the interior transmission eigenvalue problem with mixed boundary conditions is described and an efficient algorithm for computing the interior transmission eigenvalues is proposed. Finally, extensive numerical results for a variety of two-dimensional scatterers are presented to show the validity of the proposed scheme. Y1 - 2020 SN - 978-3-030-48185-8 (Hardcover) U6 - https://doi.org/10.1007/978-3-030-48186-5_9 SP - 173 EP - 195 PB - Birkhäuser CY - Cham ER - TY - JOUR A1 - Baringhaus, Ludwig A1 - Gaigall, Daniel A1 - Thiele, Jan Philipp T1 - Statistical inference for L²-distances to uniformity JF - Computational Statistics N2 - The paper deals with the asymptotic behaviour of estimators, statistical tests and confidence intervals for L²-distances to uniformity based on the empirical distribution function, the integrated empirical distribution function and the integrated empirical survival function. Approximations of power functions, confidence intervals for the L²-distances and statistical neighbourhood-of-uniformity validation tests are obtained as main applications. The finite sample behaviour of the procedures is illustrated by a simulation study. KW - Integrated empirical distribution (survival) function KW - Goodness-of-fit tests for uniformity KW - Numerical inversion of Laplace transforms KW - Coverage probability KW - Equivalence test Y1 - 2018 U6 - https://doi.org/10.1007/s00180-018-0820-0 SN - 1613-9658 VL - 2018 IS - 33 SP - 1863 EP - 1896 PB - Springer CY - Berlin ER - TY - GEN A1 - Frauenrath, Tobias A1 - Becker, Meike A1 - Hezel, Fabian A1 - Krombach, Gabriele A. A1 - Kremer, Ute A1 - Schulz-Menger, Jeanette A1 - Niendorf, Thoralf T1 - Comparison of Left Function Assessment Using Phonocardiogram and Electrocardiogram Triggered 2D SSFP CINE MR Imaging at 1.5 T and 3.0 T T2 - 2010 ISMRM-ESMRMB joint annual meeting N2 - As high-field cardiac MRI (CMR) becomes more widespread the propensity of ECG to distortions and mistriggering increases and with it the motivation for a cardiac triggering alternative. Hence, this study explores the suitability of acoustic cardiac triggering (ACT) for left ventricular (LV) function assessment in healthy subjects at 1.5T and 3.0T. Y1 - 2010 SN - 1545-4428 N1 - ISMRM-ESMRMB joint annual meeting, 1 - 7 May 2010, Stockholm, Sweden ER - TY - GEN A1 - Hezel, Fabian A1 - Frauenrath, Tobias A1 - Renz, Wolfgang A1 - Schulz-Menger, Jeanette A1 - Niendorf, Thoralf T1 - Feasibility of CINE Myocardial T2* Mapping Using Susceptibility Weighted Gradient-Echo Imaging at 7.0 T T2 - 2010 ISMRM-ESMRMB joint annual meeting N2 - This study is designed to demonstrate the promise of susceptibility weighted 2D CINE FLASH and T2* Mapping of the heart at 7T. Y1 - 2010 SN - 1545-4428 N1 - ISMRM-ESMRMB joint annual meeting, 1 - 7 May 2010, Stockholm, Sweden ER - TY - CHAP A1 - Kurz, Melanie T1 - Waffen und militärische Ausrüstung in Systemspielzeugwelten T2 - Design für Spiel, Spaß, Spannung: Gestaltung von Artefakten zum spielerischen Handeln / herausgegeben von Melanie Kurz und Thilo Schwer Y1 - 2023 SN - 978-3-89986-396-3 SP - 104 EP - 121 PB - avedition CY - Stuttgart ER - TY - GEN A1 - Pasteur, A. A1 - Ludwig, B. A1 - Tippkötter, Nils A1 - Diller, R. A1 - Kampeis, P. A1 - Ulber, Roland T1 - Aufarbeitung von β-Lactamantibiotika mittels selektiver, magnetischer Adsorbermaterialien T2 - Chemie Ingenieur Technik N2 - b-Lactame gehören zu den wirkungsvollsten Antibiotika, jedoch lassen sich viele nur schwierig fermentativ erzeugen. Ein Problem bei der fermentativen Produktion ist die Hydrolyse des Lactamrings im wässrigen Milieu. Das Ziel des von der DBU geförderten Projekts ist die selektive In-situ-Adsorption der b-Lactamantibiotika unter anschließender magnetischer Separation. Durch die Isolation im Hochgradientenmagnetseparator (HGMS) ist eine Fest-fest-flüssig-Trennung und somit ein erheblicher Zeitgewinn im Downstreamprozess möglich. Zusätzlich kommt es zur Einsparung an Lösungsmittel und Energie, was neben Reduzierung der Antibiotikahydrolyse auch in ökologischer Hinsicht einen interessanten Aspekt darstellt. Als Trägermaterial für die Adsorbermatrix werden magnetisierbare Eisenoxidpartikel eingesetzt, die in einer Silikamatrix eingebettet sind. Diese Adsorber sollen auf Selektivität in Wasser und verschiedenen Medien getestet werden. Zusätzlich werden die Abbauprodukte des b-Lactams analysiert, um eine Aussage über die Stabilisierung des Antibiotikums durch die selektiven Adsorber treffen zu können. Diese Ergebnisse werden mit kommerziell erhältlichen Adsorbern verglichen. Die Aufreinigung der Antibiotika soll direkt aus der Fermentationsbrühe erfolgen. Um die Trennung der magnetischen, selektiven Adsorber von der Biomasse zu gewährleisten, soll der HGMS in die Fermentation integriert werden. Das filamentöse Wachstum des Mikroorganismus erfordert eine Neuauslegung der Filtermatrix. KW - β-Lactame KW - Magnetische Adsorbermaterialien Y1 - 2010 U6 - https://doi.org/10.1002/cite.201050270 N1 - ProcessNet-Jahrestagung 2010 und 28. DECHEMA-Jahrestagung der Biotechnologen, 21. - 23. September 2010, Eurogress Aachen VL - 82 IS - 9 SP - 1587 PB - Wiley-VCH CY - Weinheim ER -