TY - JOUR A1 - Bayer, Robin A1 - Temiz Artmann, Aysegül A1 - Digel, Ilya A1 - Falkenstein, Julia A1 - Artmann, Gerhard A1 - Creutz, Till A1 - Hescheler, Jürgen T1 - Mechano-pharmacological testing of L-Type Ca²⁺ channel modulators via human vascular celldrum model JF - Cellular Physiology and Biochemistry N2 - Background/Aims: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology. Methods: The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. The cell generated tensions deviations in the range of a few N/m² are evaluated by the CellDrum technology. This study focuses on the dilative and contractive effects of L-type Ca²⁺ channel agonists and antagonists, respectively. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate analysis technique for the study purpose. These three operation modes are called, particular time mode" (PTM), "long term mode" (LTM) and "real-time mode" (RTM). Results: It was possible to quantify the biomechanical response of haSMCs to the addition of vasoactive agents using CellDrum technology. Due to the supplementation of 100nM Bay K8644, the tension increased approximately 10.6% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomechanical stress around 6,5% and 50nM verapamil by 2,8%, compared to the initial tension maximum. Additionally, all tested measurement modes provide similar results while focusing on different analysis parameters. Conclusion: The CellDrum technology allows highly sensitive biomechanical stress measurements of cultured haSMC monolayers. The mechanical stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m²). All tested operation modes resulted in equal findings, whereas each mode features operation-related data analysis. Y1 - 2020 U6 - https://doi.org/10.33594/000000225 SN - 1421-9778 VL - 54 SP - 371 EP - 383 PB - Cell Physiol Biochem Press CY - Düsseldorf ER - TY - JOUR A1 - Kaulen, Lars A1 - Schwabedal, Justus T. C. A1 - Schneider, Jules A1 - Ritter, Philipp A1 - Bialonski, Stephan T1 - Advanced sleep spindle identification with neural networks JF - Scientific Reports N2 - Sleep spindles are neurophysiological phenomena that appear to be linked to memory formation and other functions of the central nervous system, and that can be observed in electroencephalographic recordings (EEG) during sleep. Manually identified spindle annotations in EEG recordings suffer from substantial intra- and inter-rater variability, even if raters have been highly trained, which reduces the reliability of spindle measures as a research and diagnostic tool. The Massive Online Data Annotation (MODA) project has recently addressed this problem by forming a consensus from multiple such rating experts, thus providing a corpus of spindle annotations of enhanced quality. Based on this dataset, we present a U-Net-type deep neural network model to automatically detect sleep spindles. Our model’s performance exceeds that of the state-of-the-art detector and of most experts in the MODA dataset. We observed improved detection accuracy in subjects of all ages, including older individuals whose spindles are particularly challenging to detect reliably. Our results underline the potential of automated methods to do repetitive cumbersome tasks with super-human performance. Y1 - 2022 U6 - https://doi.org/10.1038/s41598-022-11210-y SN - 2045-2322 N1 - Corresponding author: Stephan Bialonski VL - 12 IS - Article number: 7686 SP - 1 EP - 10 PB - Springer Nature CY - London ER - TY - JOUR A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül A1 - Nishikawa, K. A1 - Artmann, Gerhard T1 - Cluster air-ion effects on bacteria and moulds JF - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2 Y1 - 2004 SN - 0932-4666 SP - 1040 EP - 1041 ER - TY - JOUR A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül A1 - Nishikawa, K. A1 - Cook, M. T1 - Bactericidal effects of plasma-generated cluster ions JF - Medical and Biological Engineering and Computing. 43 (2005), H. 6 Y1 - 2005 SN - 1741-0444 SP - 800 EP - 807 ER - TY - JOUR A1 - Digel, Ilya A1 - Kern, Inna A1 - Geenen, Eva-Maria A1 - Akimbekov, Nuraly S. T1 - Dental plaque removal by ultrasonic toothbrushes JF - dentistry journal N2 - With the variety of toothbrushes on the market, the question arises, which toothbrush is best suited to maintain oral health? This thematic review focuses first on plaque formation mechanisms and then on the plaque removal effectiveness of ultrasonic toothbrushes and their potential in preventing oral diseases like periodontitis, gingivitis, and caries. We overviewed the physical effects that occurred during brushing and tried to address the question of whether ultrasonic toothbrushes effectively reduced the microbial burden by increasing the hydrodynamic forces. The results of published studies show that electric toothbrushes, which combine ultrasonic and sonic (or acoustic and mechanic) actions, may have the most promising effect on good oral health. Existing ultrasonic/sonic toothbrush models do not significantly differ regarding the removal of dental biofilm and the reduction of gingival inflammation compared with other electrically powered toothbrushes, whereas the manual toothbrushes show a lower effectiveness. Y1 - 2020 U6 - https://doi.org/10.3390/dj8010028 SN - 2304-6767 VL - 8 IS - 28 SP - 1 EP - 13 PB - MDPI CY - Basel ER - TY - JOUR A1 - Digel, Ilya A1 - Akimbekov, Nuraly S. A1 - Turalieva, M. A1 - Mansurov, Z. A1 - Temiz Artmann, Aysegül A1 - Eshibaev, A. A1 - Zhubanova, A. T1 - Usage of Carbonized Plant Wastes for Purification of Aqueous Solutions JF - Journal of Industrial Technology and Engineering Y1 - 2013 VL - 2 IS - 07 SP - 47 EP - 54 ER - TY - JOUR A1 - Digel, Ilya A1 - Kayser, Peter A1 - Artmann, Gerhard T1 - Molecular processes in biological thermosensation JF - Journal of Biophysics. 2008 (2008) Y1 - 2008 SN - 1687-8000 SP - 1 EP - 9 ER - TY - JOUR A1 - Alexyuk, Madina A1 - Bogoyavlenskiy, Andrey A1 - Alexyuk, Pavel A1 - Moldakhanov, Yergali A1 - Berezin, Vladimir A1 - Digel, Ilya T1 - Epipelagic microbiome of the Small Aral Sea: Metagenomic structure and ecological diversity JF - MicrobiologyOpen N2 - Microbial diversity studies regarding the aquatic communities that experienced or are experiencing environmental problems are essential for the comprehension of the remediation dynamics. In this pilot study, we present data on the phylogenetic and ecological structure of microorganisms from epipelagic water samples collected in the Small Aral Sea (SAS). The raw data were generated by massive parallel sequencing using the shotgun approach. As expected, most of the identified DNA sequences belonged to Terrabacteria and Actinobacteria (40% and 37% of the total reads, respectively). The occurrence of Deinococcus-Thermus, Armatimonadetes, Chloroflexi in the epipelagic SAS waters was less anticipated. Surprising was also the detection of sequences, which are characteristic for strict anaerobes—Ignavibacteria, hydrogen-oxidizing bacteria, and archaeal methanogenic species. We suppose that the observed very broad range of phylogenetic and ecological features displayed by the SAS reads demonstrates a more intensive mixing of water masses originating from diverse ecological niches of the Aral-Syr Darya River basin than presumed before. KW - ecological structure KW - metagenomics KW - microbial diversity KW - shotgun sequencing KW - Small Aral Sea Y1 - 2021 U6 - https://doi.org/10.1002/mbo3.1142 SN - 2045-8827 N1 - Corresponding author: Ilya Digel VL - 10 IS - 1 SP - 1 EP - 10 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Akimbekov, Nuraly S. A1 - Mansurov, Zulkhair A1 - Jandosov, J. A1 - Digel, Ilya A1 - Gossmann, Matthias A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül A1 - Zhubanova, Azhar A. T1 - Wound healing activity of carbonized rice husk N2 - The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls. KW - Wundheilung KW - Epithel KW - Fibroblast KW - carbonized rice husk KW - wound healing KW - epithelization KW - human dermal fibroblasts Y1 - 2013 PB - Trans Tech Publications, Switzerland CY - Bäch ER - TY - JOUR A1 - Zhubanova, Azhar A. A1 - Aknazarov, S. K. A1 - Mansurov, Zulkhair A1 - Digel, Ilya A1 - Kozhalakova, A. A. A1 - Akimbekov, Nuraly S. A1 - O'Heras, Carlos A1 - Tazhibayeva, S. A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials N2 - Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment. KW - Kohlenstofffaser KW - Lipopolysaccharide KW - nanostrukturierte carbonisierte Pflanzenteile KW - lipopolysaccharides KW - nanostructured carbonized plant parts Y1 - 2010 ER -