TY  - JOUR
A1  - Bassam, Rasha
A1  - Hescheler, Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
A1  - Digel, Ilya
T1  - Effects of spermine NONOate and ATP on the thermal stability of hemoglobin
JF  - BMC Biophysics
N2  - Background
Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min.

Results
Major results were:
1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment,
2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and
3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature.

Conclusion
The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell.
KW  - Nitric Oxide Donor
KW  - NONOate
KW  - Circular Dichroism
KW  - Nitric Oxide
Y1  - 2012
U6  - http://dx.doi.org/10.1186/2046-1682-5-16
SN  - 2046-1682
VL  - 5
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Digel, Ilya
A1  - Embs, Jan P.
A1  - Unruh, Tobias
A1  - Tehei, M.
A1  - Zaccai, G.
A1  - Büldt, G.
A1  - Artmann, Gerhard
T1  - From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature
JF  - Biophysical Journal. 96 (2009), H. 12
Y1  - 2009
SN  - 0006-3495
SP  - 5073
EP  - 5081
PB  - Cell Press
CY  - Cambridge, Mass.
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Unruh, Tobias
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
T1  - Cytoplasmic water and hydration layer dynamics in human red blood cells
JF  - Journal of the American Chemical Society. 50 (2008), H. 130
Y1  - 2008
SN  - 1520-5126
SP  - 16852
EP  - 16853
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Kayser, Peter
A1  - Artmann, Gerhard
T1  - Molecular processes in biological thermosensation
JF  - Journal of Biophysics. 2008 (2008)
Y1  - 2008
SN  - 1687-8000
SP  - 1
EP  - 9
ER  - 
TY  - JOUR
A1  - Kotliar, Konstantin
A1  - Svetlova, O. V.
A1  - Makarov, F. N.
A1  - Zaseeva, M. V.
T1  - Morfologicheskie i funktsional'nye osobennosti resnichnogo poiaska khrustalika kak kliuchevogo ispolnitel'nogo zvena v mekhanizme akkommodatsii glaza cheloveka = Morpho-functional characteristics of lens ciliary body as a key mechanism of accommodation in
JF  - Morfologiia (Saint Petersburg, Russia). 123 (2003), H. 3
Y1  - 2003
SN  - 1026-3543
N1  - Original in Russisch, English abstract
SP  - 7
EP  - 16
PB  - -
ER  - 
TY  - JOUR
A1  - Mikielewicz, Marek
A1  - Kotliar, Konstantin
A1  - Barraquer, Rafael I.
A1  - Michael, Ralph
T1  - Air-pulse corneal applanation signal curve parameters for the characterisation of keratoconus
JF  - British Journal of Ophthalmology (eBJO)
Y1  - 2011
SN  - 1468-2079
VL  - 95
IS  - 6
SP  - 793
EP  - 798
PB  - BMJ Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Kotliar, Konstantin
A1  - Baumann, Marcus
A1  - Vilser, Walthard
A1  - Lanzl, Ines M.
T1  - Pulse wave velocity in retinal arteries of healthy volunteers
JF  - British Journal of Ophthalmology (eBJO)
Y1  - 2011
SN  - 1468-2079
VL  - 95
IS  - 11
SP  - 675
EP  - 679
PB  - BMJ Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Kotliar, Konstantin
A1  - Lanzl, Ines M.
A1  - Schmidt-Trucksäss, A.
A1  - Sitnikova, Diana
A1  - Ali, Mohammad
A1  - Blume, Katharina
A1  - Halle, Martin
A1  - Hansser, Henner
T1  - Dynamic retinal vessel response to flicker in obesity: A methodological approach
JF  - Microvascular Research
Y1  - 2011
SN  - 0026-2862
VL  - 81
IS  - 1
SP  - 123
EP  - 128
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Dashevsky, Alexey V.
A1  - Lanzl, Ines M.
A1  - Kotliar, Konstantin
T1  - Non-penetrating intracanalicular partial trabeculectomy via the ostia of Schlemm's canal
JF  - Graefe's Archive for Clinical and Experimental Ophthalmology
Y1  - 2011
SN  - 0721-832x
VL  - 249
IS  - 4
SP  - 565
EP  - 573
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Schwarz, Sonja
A1  - Kotliar, Konstantin
A1  - Eynatten, Maximilian von
A1  - Trucksaess, Arno
A1  - Burckhardt, Klaus
A1  - Lutz, Jens
A1  - Heemann, Uwe
A1  - Lanzl, Ines
T1  - Non-diabetic chronic kidney disease influences retinal microvasculature
JF  - Kidney and Blood Pressure Research
Y1  - 2009
SN  - 1423-0143
VL  - 32
IS  - 6
SP  - 428
EP  - 433
PB  - -
ER  -