TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Kirschbaum, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide
JF  - Physica status solidi (a)
N2  - Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431944
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1368
EP  - 1374
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro
JF  - Biotechnology and Bioengineering
Y1  - 2015
U6  - http://dx.doi.org/10.1002/bit.25658
SN  - 1097-0290
VL  - 112
IS  - 11
SP  - 2360
EP  - 2372
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Sievers, Susanne
A1  - Becher, Dörte
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Schweder, Thomas
A1  - Maurer, Karl-Heinz
A1  - Hecker, Michael
T1  - High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium
JF  - Proteomics
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pmic.201400504
SN  - 1615-9861
VL  - 15
IS  - 15
SP  - 2629
EP  - 2633
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Dantism, S.
A1  - Takenaga, S.
A1  - Wagner, P.
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Light-addressable Potentiometric Sensor (LAPS) Combined with Multi-chamber Structures to Investigate the Metabolic Activity of Cells
JF  - Procedia Engineering
N2  - LAPS are field-effect-based potentiometric sensors which are able to monitor analyte concentrations in a spatially resolved manner. Hence, a LAPS sensor system is a powerful device to record chemical imaging of the concentration of chemical species in an aqueous solution, chemical reactions, or the growth of cell colonies on the sensor surface, to record chemical images. In this work, multi-chamber 3D-printed structures made out of polymer (PP-ABS) were combined with LAPS chips to analyse differentially and simultaneously the metabolic activity of Escherichia coli K12 and Chinese hamster ovary (CHO) cells, and the responds of those cells to the addition of glucose solution.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.647
SN  - 1877-7058
N1  - Part of special issue "Eurosensors 2015"
VL  - 120
SP  - 384
EP  - 387
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Bäcker, Matthias
A1  - Siegert, Petra
A1  - Willenberg, H.
A1  - Poghossian, Arshak
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Detection of Adrenaline Based on Substrate Recycling Amplification
JF  - Procedia Engineering
N2  - An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.708
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 540
EP  - 543
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Schusser, Sebastian
A1  - Krischer, M.
A1  - Molin, D. G. M.
A1  - Akker, N. M. S. van den
A1  - Bäcker, Matthias
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Sensor System for in-situ and Real-time Monitoring of Polymer (bio) degradation
JF  - Procedia Engineering
N2  - A sensor system for investigating (bio)degradationprocesses of polymers is presented. The system utilizes semiconductor field-effect sensors and is capable of monitoring the degradation process in-situ and in real-time. The degradation of the polymer poly(d,l-lactic acid) is exemplarily monitored in solutions with different pH value, pH-buffer solution containing the model enzyme lipase from Rhizomucormiehei and cell-culture medium containing supernatants from stimulated and non-stimulated THP-1-derived macrophages mimicking activation of the immune system.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.815
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 948
EP  - 951
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Mariano, K.
A1  - Keusgen, M.
A1  - Selmer, Thorsten
A1  - Schöning, Michael Josef
T1  - Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes
JF  - Procedia Engineering
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.702
SN  - 1877-7058
N1  - Part of special issue "Eurosensors 2015"
VL  - 120
SP  - 532
EP  - 535
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Miyamoto, Ko-ichiro
A1  - Bing, Yu
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Schöning, Michael Josef
T1  - Visualization of Defects on a Cultured Cell Layer by Utilizing Chemical Imaging Sensor
JF  - Procedia Engineering
N2  - The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) inthe sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the defect of a cell layer brought into proximity of the sensing surface.A reduced impedance inside the defect, which was artificially formed ina cell layer, was successfully visualized in a photocurrent image.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.806
SN  - 1877-7058
N1  - Part of special issue "Eurosensors 2015"
VL  - 120
SP  - 936
EP  - 939
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bronder, Thomas
A1  - Poghossian, Arshak
A1  - Scheja, S.
A1  - Wu, Chunsheng
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer
JF  - Procedia Engineering
N2  - Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.710
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 544
EP  - 547
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Takenaga, Shoko
A1  - Schneider, Benno
A1  - Erbay, E.
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Wagner, Torsten
T1  - Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process
JF  - Physica status solidi (a)
N2  - A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201532053
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1347
EP  - 1352
PB  - Wiley
CY  - Weinheim
ER  -