TY - JOUR A1 - Borgmeier, Claudia A1 - Bongaerts, Johannes A1 - Meinhardt, Friedhelm T1 - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production JF - Journal of biotechnology N2 - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes. KW - Marker-free mutagenesis KW - Extracellular enzymes KW - Uracil-phosphoribosyltransferase KW - Hypersecretion Y1 - 2012 U6 - https://doi.org/10.1016/j.jbiotec.2012.02.011 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 159 IS - 1-2 SP - 12 EP - 20 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Borggrafe, Andreas A1 - Ohndorf, Andreas A1 - Dachwald, Bernd A1 - Seboldt, Wolfgang T1 - Analysis of interplanetary solar sail trajectories with attitude dynamics T2 - Dynamics and Control of Space Systems 2012 N2 - We present a new approach to the problem of optimal control of solar sails for low-thrust trajectory optimization. The objective was to find the required control torque magnitudes in order to steer a solar sail in interplanetary space. A new steering strategy, controlling the solar sail with generic torques applied about the spacecraft body axes, is integrated into the existing low-thrust trajectory optimization software InTrance. This software combines artificial neural networks and evolutionary algorithms to find steering strategies close to the global optimum without an initial guess. Furthermore, we implement a three rotational degree-of-freedom rigid-body attitude dynamics model to represent the solar sail in space. Two interplanetary transfers to Mars and Neptune are chosen to represent typical future solar sail mission scenarios. The results found with the new steering strategy are compared to the existing reference trajectories without attitude dynamics. The resulting control torques required to accomplish the missions are investigated, as they pose the primary requirements to a real on-board attitude control system. Y1 - 2012 SN - 978-0-87703-587-9 SP - 1553 EP - 1569 PB - Univelt Inc CY - San Diego ER - TY - CHAP A1 - Bohrn, Ulrich A1 - Stütz, Evamaria A1 - Fleischer, Maximilian A1 - Schöning, Michael Josef A1 - Wagner, Patrick T1 - Living cell-based gas sensor system for the detection of acetone in air Y1 - 2012 SN - 978-3-9813484-2-2 U6 - https://doi.org/10.5162/IMCS2012/3.2.3 SP - 269 EP - 272 ER - TY - CHAP A1 - Bohrn, Ulrich A1 - Mucha, Andreas A1 - Werner, Frederik A1 - Stütz, Evamaria A1 - Bäcker, Matthias A1 - Krumbe, Christoph A1 - Schienle, Meinrad A1 - Fleischer, Maximilian A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Detection of toxic chromium species in water using cellbased sensor systems Y1 - 2012 SN - 978-3-9813484-2-2 U6 - https://doi.org/10.5162/IMCS2012/P2.1.14 SP - 1364 EP - 1367 ER - TY - JOUR A1 - Bohrn, U. A1 - Stütz, E. A1 - Fuchs, K. A1 - Fleischer, M. A1 - Schöning, Michael Josef A1 - Wagner, P. T1 - Monitoring of irritant gas using a whole-cell-based sensor system JF - Sensors and Actuators B: Chemical N2 - Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas®), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security. Y1 - 2012 U6 - https://doi.org/10.1016/j.snb.2012.05.088 SN - 0925-4005 VL - 175 SP - 208 EP - 217 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Bohrn, U. A1 - Stütz, E. A1 - Fleischer, M. A1 - Schöning, Michael Josef A1 - Wagner, P. ED - Abdelghani, Adnane ED - Schöning, Michael Josef T1 - Towards a paradigm change – mammalian cells as sensitive biosensor layers for the detection of unexpected toxic substances in air T2 - Nanoscale Science and Technology (NS&T´12) : Proceedings Book Humboldt Kolleg ; Tunisia, 17-19 March, 2012 Y1 - 2012 SP - 44 EP - 44 ER - TY - JOUR A1 - Boettcher, Martin A1 - Haselhuhn, Angelika A1 - Jakse, Gerhard A1 - Brehmer, Bernhard A1 - Kirschner-Hermanns, Ruth T1 - Overactive bladder syndrome: an underestimated long-term problem after treatment of patients with localized prostate cancer? JF - BJU international N2 - In this study we observed courses of micturition symptoms and differentiated degrees of symptoms for each point in time while also considering the impact of bothersomeness. Our data show that not only significantly more patients who have undergone BT suffer from OAB than those who have undergone RP, but also that those affected show significantly higher values for severity of OAB symptoms throughout the whole observation period of 36 months. Our data analysis further shows that variability of OAB symptoms as well as fluctuation of severity of OAB symptoms vary to a significantly higher degree after BT than after RP. Looking only at mean figures at a given point in time clearly underestimates the underlying problem. This fact is not reflected in the literature. KW - quality of life KW - prostate cancer KW - prostatectomy KW - incontinence KW - brachytherapy Y1 - 2012 U6 - https://doi.org/10.1111/j.1464-410X.2011.10623.x SN - 1365-2176 (E-Journal); 1464-410X (E-Journal); 0007-1331 (Print); 1464-4096 (Print) VL - 109 IS - 12 SP - 1824 EP - 1830 PB - Wiley-Blackwell CY - Oxford ER - TY - CHAP A1 - Bitz, Andreas A1 - Kraff, O. A1 - Orzada, S. A1 - Maderwald, S. A1 - Brote, I. A1 - Johst, S. A1 - Ladd, E. T1 - Assessment of RF Safety of Transmit Coils at 7 Tesla by Experimental and Numerical Procedures (490.) T2 - 19th annual ISMRM scientific meeting and exhibition 2011 : Montreal, Quebec, Canada, 7 - 13 May 2011 Y1 - 2012 SN - 978-1-61839-284-8 IS - Volume 1 SP - 475 PB - Curran CY - Red Hook, NY ER - TY - BOOK A1 - Bialonski, Stephan T1 - Inferring complex networks from time series of dynamical systems: Pitfalls, misinterpretations, and possible solutions Y1 - 2012 N1 - Bonn, Rheinische Friedrich-Wilhelms-Universität Bonn, Diss., 2012 PB - Universitäts- und Landesbibliothek Bonn CY - Bonn ER - TY - CHAP A1 - Becker, Jörg A1 - Eggert, Mathias A1 - Schwittay, Sebastian ED - Mattfeld, Dirk Christian T1 - How to Evaluate the Practical Relevance of Business Process Compliance Checking Approaches? T2 - Multikonferenz Wirtschaftsinformatik 2012 - Tagungsband der MKWI 2012 Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:gbv:084-13011115376 SP - 849 EP - 862 PB - Institut für Wirtschaftsinformatik CY - Braunschweig ER -