TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements
JF  - Geophysical research letters
Y1  - 1994
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 21
IS  - Iss. 8
SP  - 689
EP  - 692
ER  - 
TY  - JOUR
A1  - Medlin, L. K.
A1  - Lange, M.
A1  - Baumann, Marcus
T1  - Genetic differentiation among three colony-forming species of Phaeocystis : further evidence for the phylogeny of the Prymnesiophyta
JF  - Phycologia
Y1  - 1994
SN  - 0031-8884
VL  - Vol. 33
IS  - Iss. 3
SP  - 199
EP  - 212
ER  - 
TY  - CHAP
A1  - Medlin, L. K.
A1  - Barker, G. L. A.
A1  - Baumann, Marcus
A1  - Hayes, P. K.
T1  - Molecular biology and systematics
T2  - The Haptophyte Algae (Special volume / Systematics Association : 51)
Y1  - 1994
SN  - 0-19-857772-9
SP  - 393
EP  - 411
PB  - Clarendon Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper “Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements”
JF  - Geophysical research letters
Y1  - 1995
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 22
IS  - Iss. 9
SP  - 1159
EP  - 1160
ER  - 
TY  - CHAP
A1  - Brandini, Frederico P.
A1  - Baumann, Marcus
T1  - The potential role of melted 'brown ice' as sources of chelators and ammonia to the surface waters of the Weddell Sea, Antarctica
T2  - Proceedings of the NIPR Symposium on Polar Biology : 10
Y1  - 1997
SN  - 0914-563X
SP  - 1
EP  - 13
CY  - Tokyo
ER  - 
TY  - PAT
A1  - Bessler, Cornelius
A1  - Maurer, Karl-Heinz
A1  - Merkel, Marion
A1  - Siegert, Petra
A1  - Wieland, Susanne
T1  - Subtilisin from Bacillus Pumilus and detergent and cleaning agents containing said novel subtilisin [US Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2009
SP  - 1
EP  - 39
PB  - USPTO; WIPO
CY  - Washington; Genf
ER  - 
TY  - JOUR
A1  - Martinez, Ronny
A1  - Jakob, Felix
A1  - Tu, Ran
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
T1  - Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution
JF  - Biotechnology and bioengineering
Y1  - 2013
SN  - 1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print)
VL  - Vol. 110
IS  - Iss. 3
SP  - 711
EP  - 720
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Jakob, Felix
A1  - Martinez, Ronny
A1  - Mandawe, John
A1  - Hellmuth, Hendrik
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
T1  - Surface charge engineering of a Bacillus gibsonii subtilisin protease
JF  - Applied microbiology and biotechnology
Y1  - 2013
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 97
IS  - Iss. 15
SP  - 6793
EP  - 6802
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Niehaus, F.
A1  - Gabor, E.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Eck, J.
T1  - Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases
JF  - Microbial biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 4
IS  - Iss. 6
SP  - 767
EP  - 776
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Heumann, S.
A1  - Karl, W.
A1  - Gerlach, J.
A1  - Leber, R.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Siegert, Petra
A1  - Lange, J.
A1  - Maurer, Karl-Heinz
A1  - Berg, G.
A1  - Guebitz, G. M.
A1  - Schwab, H.
T1  - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli
JF  - Journal of biotechnology
N2  - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3.

From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.
KW  - Alginate beads
KW  - Stenotrophomonas maltophilia
KW  - Detergent protease
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiotec.2011.09.025
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 157
IS  - 1
SP  - 140
EP  - 147
PB  - Elsevier
CY  - Amsterdam
ER  -