TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, B. A1 - Eberhardt, R. T1 - Development of an enzymatic multichannel flow injection analysis system for monitoring mammalian cell fermentations / Van der Pol, J.J.; Joksch, B.; Eberhardt, R.; Biselli, M.; Wandrey, C., Tramper, J. JF - Biosensors : fundamentals, technologies and applications ; contributions to the BMFT status seminar with international participation May, 12 to 14, 1991, Internationales Bildungs-Centrum Bogensee/Brandenburg, Germany / org. by ZIM-Zentralinstitut für Molekularbiologie, Berlin-Buch and GBF, Braunschweig. Ed. by F. Scheller Y1 - 1992 SN - 3527284370 N1 - GBF Monographs ; 17 SP - 349 EP - 352 PB - VCH CY - Weinheim ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, B. A1 - Spohn, U. T1 - On-line monitoring and control of glucose, glutamine, lactate and ammonium during a high-cell-density cultivation of hybridoma cells / van der Pol, Jens J. ; Joksch, B. ; Spohn, U. ; Biselli, M. ; Wandrey, C. JF - Proceedings of the sixth international meeting of the Japanese Association for Animal Cell Technology [JAACT 93]: Nagoya, Japan, November 9 - 12, 1993 / ed. by T. Kobayashi Y1 - 1994 SN - 0-7923-3156-7 N1 - International meeting of the Japanese Association for Animal Cell Technology, JAACT 93 ; (6 : ; 1993.11.09-12 : ; Nagoya, Japan) ; Animal cell technology, basic and applied aspects ; 6 SP - 167 EP - 170 PB - Springer Netherland CY - Berlin ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Joksch, Burkhard A1 - Galgens, Jochem T1 - On-line control of an immobilized hybridoma culture with multi-channel flow injektion analysis / Jens J. van der Pol, Burkhard Joksch, Jochem Galgens, Manfred Biselli, Cornelis D. de Gooijer, Johannes Tramper and Christian Wandrey JF - Journal of Biotechnology. 43 (1995), H. 3 Y1 - 1995 SN - 0168-1656 N1 - 3. Autor vermutlich: Jochen Gätgens SP - 229 EP - 242 ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Machnik, Marc A1 - Portela-Klein, Theresa T1 - On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells / Jens J. van der Pol, Marc Machnik, Manfred Biselli, Theresa Portela-Klein, Cornelis D. de Gooijer, Johannes Tramper und Christian Wandrey JF - Cytotechnology. 24 (1997), H. 1 Y1 - 1997 SN - 0920-9069 SP - 19 EP - 30 ER - TY - JOUR A1 - Biselli, Manfred A1 - Van der Pol, Jens J. A1 - Spohn, Uwe A1 - Eberhardt, Rolf T1 - On-line monitoring of an animal cell culture with multi-channel flow injection analysis / van der Pol, Jens J.; Spohn, Uwe ; Eberhardt, Rolf ; Gätgens, Jochen ; Biselli, Manfred ; Wandrey, Christian ; Tramper, Johannes JF - Journal of Biotechnology. 37 (1994), H. 3 Y1 - 1994 SN - 0168-1656 SP - 253 EP - 264 ER - TY - JOUR A1 - Biselli, Manfred A1 - Wandrey, Christian T1 - Reaction Mechanism of an Endoglucanase of Trichoderma Reesei QM 9414 using Cellodextrins as Substrate JF - Technology of biological processes, safety in biotechnology, applied genetic engineering : lectures held at the 5th DECHEMA Annual Meeting of Biotechnologists, May 12 - 13, 1987, [Frankfurt, Main] / [hrsg. von Dieter Behrens ...] Y1 - 1988 N1 - Jahrestagung der Biotechnologen ; <5, 1987, Frankfurt, Main> ; DECHEMA Biotechnology Conferences series ; 1 SP - 425 EP - 429 PB - VCH CY - Weinheim ER - TY - JOUR A1 - Biselli, Manfred A1 - Zeng, Steffen A1 - Dinter, Andre A1 - Eisenkrätzer, Detlef T1 - Pilot Scale Expression and Purification of Soluble Protein A Tagged β1,6N-Acetylglucosaminyltransferase in CHO Cells / Zeng, Steffen ; Dinter, Andre ; Eisenkrätzer, Detlef ; Biselli, Manfred ; Wandrey, Christian ; Berger, Eric G. JF - Biochemical and Biophysical Research Communications. 237 (1997), H. 3 Y1 - 1997 SN - 0006-291X SP - 653 EP - 658 ER - TY - JOUR A1 - Block, Franziska A1 - May, Alexander A1 - Wetzel, Katharina A1 - Adels, Klaudia A1 - Elbers, Gereon A1 - Schulze, Margit A1 - Monakhova, Yulia T1 - What is the best spectroscopic method for simultaneous analysis of organic acids and (poly)saccharides in biological matrices: Example of Aloe vera extracts? JF - Talanta Open N2 - Several species of (poly)saccharides and organic acids can be found often simultaneously in various biological matrices, e.g., fruits, plant materials, and biological fluids. The analysis of such matrices sometimes represents a challenging task. Using Aloe vera (A. vera) plant materials as an example, the performance of several spectro-scopic methods (80 MHz benchtop NMR, NIR, ATR-FTIR and UV–vis) for the simultaneous analysis of quality parameters of this plant material was compared. The determined parameters include (poly)saccharides such as aloverose, fructose and glucose as well as organic acids (malic, lactic, citric, isocitric, acetic, fumaric, benzoic and sorbic acids). 500 MHz NMR and high-performance liquid chromatography (HPLC) were used as the reference methods. UV–vis data can be used only for identification of added preservatives (benzoic and sorbic acids) and drying agent (maltodextrin) and semiquantitative analysis of malic acid. NIR and MIR spectroscopies combined with multivariate regression can deliver more informative overview of A. vera extracts being able to additionally quantify glucose, aloverose, citric, isocitric, malic, lactic acids and fructose. Low-field NMR measurements can be used for the quantification of aloverose, glucose, malic, lactic, acetic, and benzoic acids. The benchtop NMR method was successfully validated in terms of robustness, stability, precision, reproducibility and limit of detection (LOD) and quantification (LOQ), respectively. All spectroscopic techniques are useful for the screening of (poly)saccharides and organic acids in plant extracts and should be applied according to its availability as well as information and confidence required for the specific analytical goal. Benchtop NMR spectroscopy seems to be the most feasible solution for quality control of A. vera products. KW - Spectroscopy KW - (Poly)saccharides KW - Organic acids KW - Aloe vera KW - qNMR Y1 - 2023 U6 - http://dx.doi.org/10.1016/j.talo.2023.100220 SN - 2666-8319 VL - 7 IS - Art. No. 100220 SP - 1 EP - 9 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Bode, J. A1 - Bartsch, J. A1 - Boulikas, T. A1 - Iber, M. A1 - Mielke, C. A1 - Schübeler, D. A1 - Seibler, Jost A1 - Benham, C. T1 - Transcription-promoting genomic sites in mammalia: their elucidation and architectural principles JF - Gene therapy & molecular biology Y1 - 1998 SN - 1529-9120 VL - 1 IS - 1 SP - 1 EP - 29 ER - TY - JOUR A1 - Bode, Jürgen A1 - Schlake, Thomas A1 - Iber, Michaela A1 - Schübeler, Dirk A1 - Seibler, Jost A1 - Snezhkov, Evgeney A1 - Nikolaev, Lev T1 - The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes JF - Biological Chemistry Y1 - 2000 U6 - http://dx.doi.org/10.1515/BC.2000.103 SN - 1431-6730 VL - 381 IS - 9-10 SP - 801 EP - 813 ER -