TY  - JOUR
A1  - Wu, Chunsheng
A1  - Poghossian, Arshak
A1  - Bronder, Thomas
A1  - Schöning, Michael Josef
T1  - Sensing of double-stranded DNA molecules by their intrinsic molecular charge using the light-addressable potentiometric sensor
JF  - Sensors and Actuators B: Chemical
N2  - A multi-spot light-addressable potentiometric sensor (LAPS), which belongs to the family of semiconductor field-effect devices, was applied for label-free detection of double-stranded deoxyribonucleic acid (dsDNA) molecules by their intrinsic molecular charge. To reduce the distance between the DNA charge and sensor surface and thus, to enhance the electrostatic coupling between the dsDNA molecules and the LAPS, the negatively charged dsDNA molecules were electrostatically adsorbed onto the gate surface of the LAPS covered with a positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)). The surface potential changes in each spot of the LAPS, induced by the layer-by-layer adsorption of a PAH/dsDNA bilayer, were recorded by means of photocurrent-voltage and constant-photocurrent measurements. In addition, the surface morphology of the gate surface before and after consecutive electrostatic adsorption of PAH and dsDNA layers was studied by atomic force microscopy measurements. Moreover, fluorescence microscopy was used to verify the successful adsorption of dsDNA molecules onto the PAH-modified LAPS surface. A high sensor signal of 25 mV was registered after adsorption of 10 nM dsDNA molecules. The lower detection limit is down to 0.1 nM dsDNA. The obtained results demonstrate that the PAH-modified LAPS device provides a convenient and rapid platform for the direct label-free electrical detection of in-solution hybridized dsDNA molecules.
KW  - Layer-by-layer adsorption
KW  - Poly(allylamine hydrochloride)
KW  - Label-free detection
KW  - DNA biosensor
KW  - LAPS
KW  - Field effect
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.snb.2016.02.004
SN  - 0925-4005
IS  - 229
SP  - 506
EP  - 512
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Wu, Chunsheng
A1  - Poghossian, Arshak
A1  - Werner, Frederik
A1  - Bronder, Thomas
A1  - Bäcker, Matthias
A1  - Wang, Ping
A1  - Schöning, Michael Josef
T1  - An application of a scanning light-addressable potentiometric sensor for label-free DNA detection
T2  - 11. Dresdner Sensor-Symposium : 9.-11.12.2013
Y1  - 2013
SN  - 978-3-9813484-5-3
SP  - 164
EP  - 168
ER  - 
TY  - JOUR
A1  - Yoshinobu, T.
A1  - Ecken, H.
A1  - Ismail, Md.A.B.
A1  - Iwasaki, H.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Chemical imaging sensor and its application to biological systems
JF  - Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz
Y1  - 2001
SN  - 0-08-044014-2
SP  - 259
EP  - 263
PB  - Elsevier [u.a.]
CY  - Amsterdam [u.a.]
ER  - 
TY  - JOUR
A1  - Yoshinobu, T.
A1  - Ecken, H.
A1  - Poghossian, Arshak
A1  - Lüth, H.
A1  - Iwasaki, H.
A1  - Schöning, Michael Josef
T1  - Alternative sensor materials for light-addressable potentiometric sensors
JF  - Sensors and Actuators B. 76 (2001), H. 1-3
Y1  - 2001
SN  - 0925-4005
SP  - 388
EP  - 392
ER  - 
TY  - JOUR
A1  - Yoshinobu, T.
A1  - Ecken, H.
A1  - Poghossian, Arshak
A1  - Simonis, A.
A1  - Iwasaki, H.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Constant-current-mode LAPS (CLAPS) for the detection of penicillin
JF  - Electroanalysis. 13 (2001), H. 8-9
Y1  - 2001
SN  - 1040-0397
SP  - 733
EP  - 736
ER  - 
TY  - JOUR
A1  - Yoshinobu, T.
A1  - Ermelenko, Y.
A1  - Mourzina, Y.
A1  - Schöning, Michael Josef
A1  - Iwasaki, H.
A1  - Vlasov, Y.
T1  - Multi-component analysis based on the light-addressable potentiometric sensor (LAPS)
JF  - Proceedings : 11th international conference Sensor 2003; 13 - 15 May 2003, Exhibition Centre Nuremberg, Germany
Y1  - 2003
N1  - Internationale Messe mit Kongress Sensor <11, 2003, Nürnberg>
SP  - 141
EP  - 144
PB  - AMA Service
CY  - Wunstorf
ER  - 
TY  - JOUR
A1  - Yoshinobu, T.
A1  - Iwasaki, H.
A1  - Ui, Y.
A1  - Furuichi, K.
A1  - Ermelenko, Y.
A1  - Mourzina, Y.
A1  - Wagner, Torsten
A1  - Näther, Niko
A1  - Schöning, Michael Josef
T1  - The light-addressable potentiometric sensor for multi-ion sensing and imaging
JF  - Methods. 37 (2005), H. 1
Y1  - 2005
SN  - 1046-2023
SP  - 99
EP  - 102
ER  - 
TY  - JOUR
A1  - Yoshinobu, T.
A1  - Schöning, Michael Josef
A1  - Finger, F.
A1  - Moritz, W.
A1  - Iwasaki, H.
T1  - Fabrication of thin-film LAPS with amorphous silicon
JF  - Sensors. 4 (2004), H. 10
Y1  - 2004
SN  - 1424-8220
SP  - 163
EP  - 169
ER  - 
TY  - CHAP
A1  - Yoshinobu, T.
A1  - Schöning, Michael Josef
A1  - Finger, F.
A1  - Moritz, W.
A1  - Iwasaki, H.
T1  - Thin-film a-Si LAPS : Preparation and pH sensitivity
T2  - Eurosensors XVII : the 17th European Conference on Solid-State Transducers ; University of Minho, Guimarães, Portugal, September 21 - 24, 2003
Y1  - 2003
SP  - 88
EP  - 89
ER  - 
TY  - JOUR
A1  - Yoshinobu, T.
A1  - Schöning, Michael Josef
A1  - Otto, R.
A1  - Furuichi, K.
A1  - Mourzina, Y.
A1  - Ermelenko, Y.
A1  - Iwasaki, H.
T1  - Portable light-addressable potentiometric sensor (LAPS) for multisensor applications
JF  - Sensors and Actuators B. 95 (2003), H. 1-3
Y1  - 2003
SN  - 0925-4005
SP  - 352
EP  - 356
ER  -