TY - JOUR A1 - Öhlschläger, Peter A1 - Steinberg, Thorsten A1 - Sehr, Peter A1 - Osen, Wolfram T1 - Modification of HPV 16 E7 genes: correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice / Steinberg, Thorsten ; Öhlschläger, Peter ; Sehr, Peter ; Osen, Wolfram ; Gissmann, Lutz JF - Vaccine. 23 (2005), H. 9 Y1 - 2005 SN - 0264-410X SP - 1149 EP - 1157 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Spies, Elmar A1 - Alvarez, Gerardo A1 - Quetting, Michael A1 - Groettrup, Marcus T1 - The combination of TLR-9 adjuvantation and electroporation-mediated delivery enhances in vivo antitumor responses after vaccination with HPV-16 E7 encoding DNA JF - International Journal of Cancer. 128 (2011), H. 2 Y1 - 2011 SN - 1097-0215 SP - 473 EP - 481 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Quetting, Michael A1 - Alvarez, Gerardo A1 - Dürst, Matthias A1 - Gissmann, Lutz A1 - Kaufmann, Andreas M. T1 - Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants JF - International Journal of Cancer Y1 - 2009 SN - 1097-0215 VL - 125 IS - 1 SP - 189 EP - 198 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Pes, Michaela A1 - Osen, Wolfram A1 - Dürst, Matthias T1 - An improved rearranged Human Papillomavirus Type 16 E7 DNA vaccine candidate (HPV-16 E7SH) induces an E7 wildtype-specific T cell response / Öhlschläger, Peter ; Pes, Michaela ; Osen, Wolfram ; Dürst, Matthias ; Schneider, Achim ; Gissmann, Lutz ; Kaufman JF - Vaccine. 24 (2006), H. 15 Y1 - 2006 SN - 0264-410X SP - 2880 EP - 2893 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Osen, Wolfram A1 - Peiler, Tanja A1 - Caldeira, Sandra T1 - A DNA vaccine based on a shuffled E7 oncogene of the human papillomavirus type 16 (HPV 16) induces E7-specific cytotoxic T cells but lacks transforming activity / Osen, Wolfram ; Peiler, Tanja ; Öhlschläger, Peter ; Caldeira, Sandra ; Faath, Stefan ; Mich JF - Vaccine. 19 (2001), H. 20 Y1 - 2001 SN - 0264-410X SP - 4276 EP - 4286 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Osen, Wolfram A1 - Dell, Kerstin A1 - Faath, Stefan T1 - Human papillomavirus type 16 L1 capsomeres induce L1-specific cytotoxic T lymphocytes and tumor regression in C57BL/6 mice / Öhlschläger, Peter ; Osen, Wolfram ; Dell, Kerstin ; Faath, Stefan ; Garcea Robert L: ; Jochmus, Ingrid ; Müller, Martin, Pawlita, JF - Journal of Virology. 77 (2003), H. 8 Y1 - 2003 SN - 1098-5514 SP - 4635 EP - 4645 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Michel, Nico A1 - Osen, Wolfram A1 - Freyschmidt, Eva-Jasmin T1 - T cell response to human papillomavirus 16 E7 in mice: comparison of Cr release assay, intracellular IFN-gamma production, ELISPOT and tetramer staining / Michel, Nico ; Öhlschläger, Peter ; Osen, Wolfram ; Freyschmidt, Eva-Jasmin ; Gutöhrlein, Heidrun ; JF - Intervirology. 45 (2002) Y1 - 2002 SN - 1423-0100 SP - 290 EP - 299 ER - TY - JOUR A1 - Öhlschläger, Peter A1 - Corvinus, Florian M. A1 - Orth, Carina A1 - Moriggl, Richard T1 - Persistent STAT3 activation in colon cancer is associated with enhanced cell proliferation and tumor growth / Corvinus, Florian, Moriggl, Richard ; Tsareva, Svetlana A. ; Wagner, Stefan ; Pfitzner, Edith B. ; Baus, Daniela ; Kaufmann, Roland : Huber, Luka JF - Neoplasia. 7 (2005), H. 6 Y1 - 2005 SN - 1476-5586 SP - 545 EP - 555 ER - TY - JOUR A1 - Zientz, Evelyn A1 - Bongaerts, Johannes A1 - Unden, Gottfried T1 - Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system JF - Journal of bacteriology Y1 - 1998 SN - 1098-5530 (E-Journal); 0021-9193 (Print) VL - Vol. 180 IS - No. 20 SP - 5421 EP - 5425 ER - TY - JOUR A1 - Zhantlessova, Sirina A1 - Savitskaya, Irina A1 - Kistaubayeva, Aida A1 - Ignatova, Ludmila A1 - Talipova, Aizhan A1 - Pogrebnjak, Alexander A1 - Digel, Ilya T1 - Advanced “Green” prebiotic composite of bacterial cellulose/pullulan based on synthetic biology-powered microbial coculture strategy JF - Polymers N2 - Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for “grafting” of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality. KW - coculture KW - pullulan KW - exopolysaccharides KW - prebiotic KW - bacterial cellulose Y1 - 2022 U6 - http://dx.doi.org/10.3390/polym14153224 SN - 2073-4360 N1 - This article belongs to the Special Issue "Cellulose Based Composites" VL - 14 IS - 15 PB - MDPI CY - Basel ER -