TY  - CHAP
A1  - Yoshinobu, Tatsuo
A1  - Krause, Steffi
A1  - Miyamoto, Ko-ichiro
A1  - Werner, Frederik
A1  - Poghossian, Arshak
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - (Bio-)chemical Sensing and Imaging by LAPS and SPIM
T2  - Label-free biosensing: advanced materials, devices and applications
N2  - The light-addressable potentiometric sensor (LAPS) and scanning photo-induced impedance microscopy (SPIM) are two closely related methods to visualise the distributions of chemical species and impedance, respectively, at the interface between the sensing surface and the sample solution. They both have the same field-effect structure based on a semiconductor, which allows spatially resolved and label-free measurement of chemical species and impedance in the form of a photocurrent signal generated by a scanning light beam. In this article, the principles and various operation modes of LAPS and SPIM, functionalisation of the sensing surface for measuring various species, LAPS-based chemical imaging and high-resolution sensors based on silicon-on-sapphire substrates are described and discussed, focusing on their technical details and prospective applications.
KW  - Chemical imaging
KW  - Field-effect device
KW  - Light-addressable potentiometric sensor
KW  - Potentiometry
Y1  - 2018
SN  - 978-3-319-75219-8
SP  - 103
EP  - 132
PB  - Springer
CY  - Cham
ER  - 
TY  - CHAP
A1  - Schöning, Michael Josef
A1  - Wagner, Torsten
A1  - Poghossian, Arshak
A1  - Miyamoto, K.I.
A1  - Werner, C.F.
A1  - Krause, S.
A1  - Yoshinobu, T.
T1  - Light-addressable potentiometric sensors for (bio-)chemical sensing and imaging
T2  - Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry. Vol. 7
Y1  - 2018
SN  - 9780128097397
SP  - 295
EP  - 308
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - Enzyme und Biosensorik
T2  - Einführung in die Enzymtechnologie
N2  - Enzymbasierte Biosensoren finden seit mehr als fünf Jahrzehnten einen prosperierenden Wachstumsmarkt und werden zunehmend auch in biotechnologischen Prozessen eingesetzt. In diesem Kapitel werden, ausgehend vom Sensorbegriff und typischen Kenngrößen für Biosensoren (Abschn. 18.1), elektrochemische Enzym-Biosensoren vorgestellt und deren typischen Einsatzgebiete diskutiert (Abschn. 18.2). Ein Blick über den „Tellerrand“ hinaus zeigt alternative Transduktorprinzipien (Abschn. 18.3) und führt abschließend in aktuelle Forschungstrends ein (Abschn. 18.4).
Y1  - 2018
SN  - 978-3-662-57619-9
U6  - http://dx.doi.org/10.1007/978-3-662-57619-9_18
SP  - 323
EP  - 347
PB  - Springer Spektrum
CY  - Berlin
ER  - 
TY  - CHAP
A1  - Koch, Claudia
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Schöning, Michael Josef
ED  - Wege, Christina
T1  - TMV-Based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications
T2  - Virus-Derived Nanoparticles for Advanced Technologies
N2  - Nanotubular tobacco mosaic virus (TMV) particles and RNA-free lower-order coat protein (CP) aggregates have been employed as enzyme carriers in different diagnostic layouts and compared for their influence on biosensor performance. In the following, we describe a label-free electrochemical biosensor for improved glucose detection by use of TMV adapters and the enzyme glucose oxidase (GOD). A specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CP aggregates was achieved via bioaffinity binding. Glucose sensors with adsorptively immobilized [SA]-GOD, and with [SA]-GOD cross-linked with glutardialdehyde, respectively, were tested in parallel on the same sensor chip. Comparison of these sensors revealed that TMV adapters enhanced the amperometric glucose detection remarkably, conveying highest sensitivity, an extended linear detection range and fastest response times. These results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for applications in biosensorics and biochips. Here, we describe the fabrication and use of amperometric sensor chips combining an array of circular Pt electrodes, their loading with GOD-modified TMV nanotubes (and other GOD immobilization methods), and the subsequent investigations of the sensor performance.
KW  - Tobacco mosaic virus (TMV)
KW  - Coat protein
KW  - Enzyme nanocarrier
KW  - Glucose biosensor
KW  - Glucose oxidase
Y1  - 2018
SN  - 978-1-4939-7808-3
U6  - http://dx.doi.org/10.1007/978-1-4939-7808-3
N1  - Methods in Molecular Biology, vol 1776
SP  - 553
EP  - 568
PB  - Humana Press
CY  - New York, NY
ER  -