TY - CHAP A1 - Pijanowska, Dorota G. A1 - Remiszewska, Elzbieta A1 - Pederzolli, Cecilia A1 - Lunelli, Lorenzo A1 - Vendano, Michele A1 - Canteri, Roberto A1 - Dudzinski, Konrad A1 - Kruk, Jerzy A1 - Torbicz, Wladyslaw T1 - Surface modification for microreactor fabrication N2 - In this paper, methods of surface modification of different supports, i.e. glass and polymeric beads for enzyme immobilisation are described. The developed method of enzyme immobilisation is based on Schiff’s base formation between the amino groups on the enzyme surface and the aldehyde groups on the chemically modified surface of the supports. The surface of silicon modified by APTS and GOPS with immobilised enzyme was characterised by atomic force microscopy (AFM), time-of-flight secondary ion mass spectroscopy (ToF-SIMS) and infrared spectroscopy (FTIR). The supports with immobilised enzyme (urease) were also tested in combination with microreactors fabricated in silicon and Perspex, operating in a flow-through system. For microreactors filled with urease immobilised on glass beads (Sigma) and on polymeric beads (PAN), a very high and stable signal (pH change) was obtained. The developed method of urease immobilisation can be stated to be very effective. KW - Biosensor KW - Microreactors KW - surface modification KW - enzyme immobilisation KW - lab-on-a-chip Y1 - 2006 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1480 ER - TY - CHAP A1 - Pijanowska, Dorota G. A1 - Remiszewska, Elzbieta T1 - pH-based detection of phenylalnine by potentiometric and colorimetric methods N2 - In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD+, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration. KW - Biosensor KW - Phenylalanine determination KW - enzymatic methods KW - pH-based biosensing Y1 - 2006 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1536 ER - TY - JOUR A1 - Pietschmann, Bernd P. A1 - Vahs, Dietmar T1 - Change Management - Wie Sie Ihr Unternehmen fit für die Zukunft machen. Teil 1: Erfolgsfaktoren von Veränderungsprozessen JF - News / ASU ; BJU : von Unternehmern für Unternehmer. Ausgabe Baden-Württemberg. 13 (1996), H. 5 Y1 - 1996 SP - 76 EP - 79 ER - TY - JOUR A1 - Pietschmann, Bernd P. A1 - Vahs, Dietmar T1 - Change Management - Wie Sie Ihr Unternehmen fit für die Zukunft machen. Teil 2: Prozeßmanagement - Von starren Strukturen zu flexiblen Prozessen JF - News / ASU ; BJU : von Unternehmern für Unternehmer. Ausgabe Baden-Württemberg. 13 (1996), H. 6 Y1 - 1996 SP - 84 EP - 86 ER - TY - JOUR A1 - Pietschmann, Bernd P. A1 - Ruhtz, Vanessa T1 - Knowledge Management JF - Personal : Zeitschrift für Human Resource Management. 53 (2001), H. 5 Y1 - 2001 SN - 0031-5605 SP - 242 EP - 249 ER - TY - JOUR A1 - Pietsch, Wolfram A1 - Taudes, A. T1 - Groupware Support for Interorganizational Courseware-Development. A Case Study / Pietsch, W. , Taudes, A. JF - IM : die Fachzeitschrift für Information Management & Consulting (1994) Y1 - 1994 SN - 0930-5181, 1616-1017 SP - 12 EP - 18 ER - TY - JOUR A1 - Pietsch, Wolfram A1 - Steinbauer, Dieter T1 - Business Process Reengineering JF - Wirtschaftsinformatik. 36 (1994), H. 5 Y1 - 1994 SN - 0937-6429 SP - 502 EP - 505 ER - TY - JOUR A1 - Pietsch, Wolfram A1 - Kurbel, Karl A1 - Jung, R. T1 - Modeling Knowledge about Long-term IS-Integration and Integration-oriented Reengineering with KADS / Kurbel, K., Jung, R., Pietsch, W. JF - Distributed information systems in business / W. König ... (eds.) Y1 - 1996 SN - 3-540-61094-4 PB - Springer CY - Berlin [u.a.] ER - TY - JOUR A1 - Pietsch, Wolfram A1 - Kurbel, Karl T1 - A Cooperative Work Environment for Evolutionary Software Development / Kurbel, K., Pietsch, W. JF - Multi-user interfaces and applications : proceedings of the IFIP WG 8.4 Conference on Multi-User Interfaces and Applications, Heraklion, Crete, Greece, 24 - 26 September, 1990 / ed. by Simon Gibbs ... Y1 - 1990 SN - 0-444-88760-1 N1 - Conference on Multi-User Interfaces and Applications <1990, Hērakleion> SP - 115 EP - 127 PB - North-Holland Publ. CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Pietsch, Wolfram A1 - Kurbel, Karl T1 - A Portable Implementation of Index Sequential Input-Output [Part 1] / Kurbel, Karl; Pietsch, W. JF - Ada letters. 6 (1986), H. 2 Y1 - 1986 SN - 0736-721X SP - 29 EP - 40 ER -