TY  - JOUR
A1  - Werfel, Stanislas
A1  - Günthner, Roman
A1  - Hapfelmeier, Alexander
A1  - Hanssen, Henner
A1  - Kotliar, Konstantin
A1  - Heemann, Uwe
A1  - Schmaderer, Christoph
ED  - Guzik, Tomasz J.
T1  - Identification of cardiovascular high-risk groups from dynamic retinal vessel signals using untargeted machine learning
JF  - Cardiovascular Research
N2  - Dynamic retinal vessel analysis (DVA) provides a non-invasive way to assess microvascular function in patients and potentially to improve predictions of individual cardiovascular (CV) risk. The aim of our study was to use untargeted machine learning on DVA in order to improve CV mortality prediction and identify corresponding response alterations.
KW  - Machine learning
KW  - Retinal vessels
KW  - Microcirculation
KW  - Haemodialysis
KW  - Myocardial infarction and cardiac death
Y1  - 2022
U6  - http://dx.doi.org/10.1093/cvr/cvab040
SN  - 0008-6363
VL  - 118
IS  - 2
SP  - 612
EP  - 621
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - CHAP
A1  - Wendorff, Marion
A1  - Eggert, Thorsten
A1  - Pohl, Martina
A1  - Dresen, Carola
A1  - Müller, Michael
A1  - Jaeger, Karl-Erich
A1  - Sprenger, Georg A.
A1  - Schürmann, Melanie
A1  - Schürmann, Martin
A1  - Johnen, Sandra
A1  - Sprenger, Gerda
A1  - Sahm, Hermann
A1  - Inoue, Tomoyuki
A1  - Schörken, Ulrich
A1  - Breittaupt, Holger
A1  - Frölich, Bettina
A1  - Heim, Petra
A1  - Iding, Hans
A1  - Juchem, Bettina
A1  - Siegert, Petra
A1  - Kula, Maria-Regina
A1  - Weckbecker, Andrea
A1  - Hummel, Werner
A1  - Fessner, Wolf-Dieter
A1  - Elling, Lothar
A1  - Wolberg, Michael
A1  - Bode, Silke
A1  - Feldmann, Ralf
A1  - Geilenkirchen, Petra
A1  - Schubert, Thomas
A1  - Walter, Lydia
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Kolter-Jung, Doris
A1  - Nitsche, Adam
A1  - Dünkelmann, Pascal
A1  - Cosp, Annabel
A1  - Lingen, Bettina
T1  - Catalytic asymmetric synthesis : section 2.2
T2  - Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ...
Y1  - 2007
SN  - 978-3-527-31473-7
SP  - 298
EP  - 413
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Wendlandt, Tim
A1  - Koch, Claudia
A1  - Britz, Beate
A1  - Liedek, Anke
A1  - Schmidt, Nora
A1  - Werner, Stefan
A1  - Gleba, Yuri
A1  - Vahidpour, Farnoosh
A1  - Welden, Melanie
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
T1  - Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System
JF  - Viruses
N2  - Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.
KW  - biosensor
KW  - horseradish peroxidase (HRP)
KW  - glucose oxidase (GOx)
KW  - enzyme cascade
KW  - turnip vein clearing virus (TVCV)
KW  - tobacco mosaic virus (TMV)
Y1  - 2023
U6  - http://dx.doi.org/doi.org/10.3390/v15091951
SN  - 1999-4915
N1  - This article belongs to the Special Issue "Tobamoviruses 2023"
VL  - 9
IS  - 15
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Wemhöner, Carsten
A1  - Hafner, Bernd
A1  - Schwarzer, Klemens
T1  - Simulation of solar thermal systems with CARNOT blockset in the environment MATLAB-Simulink
Y1  - 2000
SP  - 1
EP  - 6
ER  - 
TY  - CHAP
A1  - Wellmer, Georg
A1  - Chen, B.-H.
A1  - Braun, Carsten
A1  - Ballmann, Josef
T1  - Numerical prediction of aeroelastic effects on twin-sting-rig mounted models for rear fuselage and empennage flow investigation in transonic windtunnel
T2  - Proceedings / IFASD 2007, CEAS/AIAA/KTH International Forum on Aeroelasticity and Structural Dynamics, June 18 - 21, 2007, Stockholm, Sweden
Y1  - 2007
PB  - KTH
CY  - Stockholm
ER  - 
TY  - JOUR
A1  - Welden, Rene
A1  - Schöning, Michael Josef
A1  - Wagner, Patrick H.
A1  - Wagner, Torsten
T1  - Light-Addressable Electrodes for Dynamic and Flexible Addressing of Biological Systems and Electrochemical Reactions
JF  - Sensors
N2  - In this review article, we are going to present an overview on possible applications of light-addressable electrodes (LAE) as actuator/manipulation devices besides classical electrode structures. For LAEs, the electrode material consists of a semiconductor. Illumination with a light source with the appropiate wavelength leads to the generation of electron-hole pairs which can be utilized for further photoelectrochemical reaction. Due to recent progress in light-projection technologies, highly dynamic and flexible illumination patterns can be generated, opening new possibilities for light-addressable electrodes. A short introduction on semiconductor–electrolyte interfaces with light stimulation is given together with electrode-design approaches. Towards applications, the stimulation of cells with different electrode materials and fabrication designs is explained, followed by analyte-manipulation strategies and spatially resolved photoelectrochemical deposition of different material types.
Y1  - 2020
U6  - http://dx.doi.org/10.3390/s20061680
SN  - 1424-8220
VL  - 20
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Welden, Rene
A1  - Scheja, Sabrina
A1  - Schöning, Michael Josef
A1  - Wagner, Patrick
A1  - Wagner, Torsten
T1  - Electrochemical Evaluation of Light‐Addressable Electrodes Based on TiO2 for the Integration in Lab‐on‐Chip Systems
JF  - physica status solidi a : applications and materials sciences
N2  - In lab-on-chip systems, electrodes are important for the manipulation (e.g., cell stimulation, electrolysis) within such systems. An alternative to commonly used electrode structures can be a light-addressable electrode. Here, due to the photoelectric effect, the conducting area can be adjusted by modification of the illumination area which enables a flexible control of the electrode. In this work, titanium dioxide based light-addressable electrodes are fabricated by a sol–gel technique and a spin-coating process, to deposit a thin film on a fluorine-doped tin oxide glass. To characterize the fabricated electrodes, the thickness, and morphological structure are measured by a profilometer and a scanning electron microscope. For the electrochemical behavior, the dark current and the photocurrent are determined for various film thicknesses. For the spatial resolution behavior, the dependency of the photocurrent while changing the area of the illuminated area is studied. Furthermore, the addressing of single fluid compartments in a three-chamber system, which is added to the electrode, is demonstrated.
Y1  - 2018
U6  - http://dx.doi.org/10.1002/pssa.201800150
SN  - 1862-6319
VL  - 215
IS  - 15
SP  - Article number 1800150
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Welden, Rene
A1  - Nagamine Komesu, Cindy A.
A1  - Wagner, Patrick H.
A1  - Schöning, Michael Josef
A1  - Wagner, Torsten
T1  - Photoelectrochemical enzymatic penicillin biosensor: A proof-of-concept experiment
JF  - Electrochemical Science Advances
N2  - Photoelectrochemical (PEC) biosensors are a rather novel type of biosensors thatutilizelighttoprovideinformationaboutthecompositionofananalyte,enablinglight-controlled multi-analyte measurements. For enzymatic PEC biosensors,amperometric detection principles are already known in the literature. In con-trast, there is only a little information on H+-ion sensitive PEC biosensors. Inthis work, we demonstrate the detection of H+ions emerged by H+-generatingenzymes, exemplarily demonstrated with penicillinase as a model enzyme on atitanium dioxide photoanode. First, we describe the pH sensitivity of the sensorand study possible photoelectrocatalytic reactions with penicillin. Second, weshow the enzymatic PEC detection of penicillin.
KW  - enzymatic biosensor
KW  - penicillin
KW  - penicillinase
KW  - photoelectrochemistry
KW  - titanium dioxide photoanode
Y1  - 2021
U6  - http://dx.doi.org/10.1002/elsa.202100131
SN  - 2698-5977
N1  - Corresponding auhtor: Michael J. Schöning
VL  - 2
IS  - 4
SP  - 1
EP  - 5
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Welden, Rene
A1  - Jablonski, Melanie
A1  - Wege, Christina
A1  - Keusgen, Michael
A1  - Wagner, Patrick Hermann
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
T1  - Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase
JF  - Biosensors
N2  - The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.
KW  - microfluidics
KW  - enzyme kinetics
KW  - actuator-sensor system
KW  - light-addressable electrode
KW  - light-addressable potentiometric sensor
Y1  - 2021
U6  - http://dx.doi.org/10.3390/bios11060171
SN  - 2079-6374
N1  - This article belongs to the Special Issue "Selected Papers from the 1st International Electronic Conference on Biosensors (IECB 2020)"
VL  - 11
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - CHAP
A1  - Welden, Melanie
A1  - Severins, Robin
A1  - Poghossian, Arshak
A1  - Wege, Christina
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Studying the immobilization of acetoin reductase with Tobacco mosaic virus particles on capacitive field-effect sensors
T2  - 2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN)
N2  - A capacitive electrolyte-insulator-semiconductor (EISCAP) biosensor modified with Tobacco mosaic virus (TMV) particles for the detection of acetoin is presented. The enzyme acetoin reductase (AR) was immobilized on the surface of the EISCAP using TMV particles as nanoscaffolds. The study focused on the optimization of the TMV-assisted AR immobilization on the Ta 2 O 5 -gate EISCAP surface. The TMV-assisted acetoin EISCAPs were electrochemically characterized by means of leakage-current, capacitance-voltage, and constant-capacitance measurements. The TMV-modified transducer surface was studied via scanning electron microscopy.
KW  - Tobacco mosaic virus
KW  - acetoin
KW  - capacitive field-effect biosensor
KW  - enzyme immobilization
Y1  - 2022
SN  - 978-1-6654-5860-3 (Online)
SN  - 978-1-6654-5861-0 (Print)
U6  - http://dx.doi.org/10.1109/ISOEN54820.2022.9789657
N1  - IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), 29 May 2022 - 01 June 2022, Aveiro, Portugal.
PB  - IEEE
ER  -