TY - JOUR A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Bäcker, Matthias A1 - Schöning, Michael Josef T1 - Label-free electrical detection of DNA with a multi-spot LAPS: First step towards light-addressable DNA chips JF - Physica status solidi A : Applications and materials science N2 - A multi-spot (4 × 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent–voltage (I–V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I–V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift (∼5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge. Y1 - 2014 U6 - http://dx.doi.org/10.1002/pssa.201330442 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1423 EP - 1428 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer T2 - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250) Y1 - 2014 SN - 978-3-8007-3622-5 SP - 1 EP - 4 PB - VDE-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Wrede, Oliver T1 - Mnemotechnik in graphischen Benutzeroberflächen JF - Form / Form-Diskurs : Zeitschrift für Design und Theorie. 1 (1996), H. 2 Y1 - 1996 ER - TY - JOUR A1 - Wrede, Oliver T1 - Wer nicht hören will muss sehen : ein Plädoyer für eine entwurfsbezogene Bildwissenschaft JF - Boxhorn. 12 (2005), H. Sehsturz Y1 - 2005 SP - 24 EP - 25 ER - TY - JOUR A1 - Wrede, Oliver T1 - Continuity in travel information JF - Information Design Journal N2 - This article discusses the contrast between the information transportation companies provide to travellers and that of their brand messaging. Companies’ brand messaging often portrays the service they provide as pleasant, stress free and perfect. Customers and users of the service, on the other hand, often describe their experience of the service as a negative one. This article suggests that the brand value would be greater if transportation companies paid more attention to the users’ experience when designing their information systems, particularly in worst case scenarios. Y1 - 2016 U6 - http://dx.doi.org/10.1075/idj.22.2.09wre SN - 0142-5471 N1 - Gedruckt vorhanden in der Bereichsbibliothek Boxgraben unter 53 Z 971-2016 (Magazin) VL - 22 IS - 2 SP - 172 EP - 178 PB - John Benjamins CY - Amsterdam ER - TY - BOOK A1 - Wosnitza, Franz A1 - Hilgers, Hans Gerd T1 - Energieeffizienz und Energiemanagement : Ein Überblick heutiger Möglichkeiten und Notwendigkeiten Y1 - 2012 SN - 978-3-8348-8671-2 PB - Vieweg + Teubner CY - Wiesbaden ER - TY - BOOK A1 - Wosnitza, Franz A1 - Hilgers, Hans Gerd T1 - Energieeffizienz und Energiemanagement : ein Überblick heutiger Möglichkeiten und Notwendigkeiten Y1 - 2012 SN - 978-3-8348-1941-3 N1 - eISBN 978-3-8348-8671-2 PB - Springer Spektrum CY - Wiesbaden ER - TY - BOOK A1 - Wosnitza, Franz T1 - Bau eines HF-Empfangsteiles, Bild-Ton-ZF-Verstärkers und Demodulators, sowie eines Bildträgertonempfangsteiles für Schmalbandfernsehen für den ATV-Fernsehumsetzer DK 0 HJ / Betreuer: Erich Vogelsang Y1 - 1976 N1 - Aachen, Fachhochschule, Diplomarbeit 1976 ER - TY - BOOK A1 - Wosnitza, Franz T1 - Freifeldmessungen an Violinen und gehörgemäße Bewertungen ihrer Frequenzen Y1 - 1980 N1 - Aachen, Technische Hochschule, Diplomarbeit ca. 1980 ; Institut für Technische Akustik ER - TY - BOOK A1 - Wosnitza, Franz T1 - Ein Testbarkeitsanalysemaß zur Architekturbewertung programmierbarer Schaltnetze / von Franz Wosnitza Y1 - 1986 N1 - Aachen, Techn. Hochsch., Diss., 1986 ER -