TY  - JOUR
A1  - Brahma, Aischarya
A1  - Musio, Biagia
A1  - Ismayilova, Uliviya
A1  - Nikbin, Nikzad
A1  - Kamptmann, Sonja B.
A1  - Siegert, Petra
A1  - Jeromin, Günter Erich
A1  - Ley, Steven
A1  - Pohl, Martina
T1  - An orthogonal biocatalytic approach for the safe generation and use of HCN in a multi-step continuous preparation of chiral O-acetylcyanohydrins
JF  - Synlett
Y1  - 2015
U6  - https://doi.org/10.1055/s-0035-1560644
SN  - 0936-5214 (Print) ; 1437-2096 (e-Journal)
IS  - Publ. online 29.09.2015
PB  - Thieme
CY  - Stuttgart
ER  - 
TY  - JOUR
A1  - Borgmeier, Claudia
A1  - Bongaerts, Johannes
A1  - Meinhardt, Friedhelm
T1  - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production
JF  - Journal of biotechnology
N2  - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.
KW  - Marker-free mutagenesis
KW  - Extracellular enzymes
KW  - Uracil-phosphoribosyltransferase
KW  - Hypersecretion
Y1  - 2012
U6  - https://doi.org/10.1016/j.jbiotec.2012.02.011
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 159
IS  - 1-2
SP  - 12
EP  - 20
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bongaerts, Johannes
A1  - Esser, Simon
A1  - Lorbach, Volker
A1  - Al-Momani, Lóay
A1  - Müller, Michael A.
A1  - Franke, Dirk
A1  - Grondal, Christoph
A1  - Kurutsch, Anja
A1  - Bujnicki, Robert
A1  - Takors, Ralf
A1  - Raeven, Leon
A1  - Wubbolts, Marcel
A1  - Bovenberg, Roel
A1  - Nieger, Martin
A1  - Schürmann, Melanie
A1  - Trachtmann, Natalie
A1  - Kozak, Stefan
A1  - Sprenger, Georg A.
A1  - Müller, Michael
T1  - Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis
JF  - Angewandte Chemie International Edition
Y1  - 2011
SN  - 1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)
VL  - Vol. 50
IS  - Iss. 34
SP  - 7781
EP  - 7786
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Bohrn, Ulrich
A1  - Stütz, Evamaria
A1  - Fleischer, Maximilian
A1  - Schöning, Michael Josef
A1  - Wagner, Patrick
T1  - Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1345
EP  - 1350
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Bohrn, Ulrich
A1  - Stütz, Evamaria
A1  - Fleischer, Maximilian
A1  - Schöning, Michael Josef
A1  - Wagner, Patrick
T1  - Using a cell-based gas biosensor for investigation of adverse effects of acetone vapors in vitro
JF  - Biosensors and Bioelectronics. 40 (2013), H. 1
Y1  - 2013
SN  - 0956-5663
SP  - 393
EP  - 400
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Bohrn, Ulrich
A1  - Stütz, Evamaria
A1  - Fleischer, Maximilian
A1  - Schöning, Michael Josef
A1  - Wagner, Patrick
T1  - Living cell-based gas sensor system for the detection of acetone in air
Y1  - 2012
SN  - 978-3-9813484-2-2
U6  - https://doi.org/10.5162/IMCS2012/3.2.3
SP  - 269
EP  - 272
ER  - 
TY  - JOUR
A1  - Bohrn, Ulrich
A1  - Stütz, Evamaria
A1  - Fleischer, Maximilian
A1  - Schöning, Michael Josef
T1  - Real-time detection of CO by eukaryotic cells
JF  - Procedia Engineering
N2  - In this contribution, we focus on the detection of toxic gases with living eukaryotic cells. A cell-based gas sensor system, able to measure the effects of direct exposure of gases to cells in real-time, was set up. Impedance data as well as oxygen consumption of Chinese hamster lung fibroblast cells (V79) were analysed upon exposure to carbon monoxide (CO). The CO (diluted in wet synthetic air) affects the cell respiration as indicated by an attenuated respiration signal after the CO exposure as well as an instant increase of the capacitive part of the impedance signal during the gas exposure.
Y1  - 2010
SN  - 1877-7058
N1  - Eurosensor XXIV Conference, 5-8 September 2010, Linz, Austria
VL  - 5
SP  - 17
EP  - 20
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bohrn, Ulrich
A1  - Mucha, Andreas
A1  - Werner, Frederik
A1  - Trattner, Barbara
A1  - Bäcker, Matthias
A1  - Krumbe, Christoph
A1  - Schienle, Meinrad
A1  - Stütz, Evamaria
A1  - Schmitt-Landsiedel, Doris
A1  - Fleischer, Maximilian
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - A critical comparison of cell-based sensor systems for the detection of Cr (VI) in aquatic environment
JF  - Sensors and actuators. B: Chemical
Y1  - 2013
SN  - 1873-3077 (E-Journal); 0925-4005 (Print)
VL  - Vol. 182
SP  - 58
EP  - 65
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Bohrn, Ulrich
A1  - Mucha, Andreas
A1  - Werner, Frederik
A1  - Stütz, Evamaria
A1  - Bäcker, Matthias
A1  - Krumbe, Christoph
A1  - Schienle, Meinrad
A1  - Fleischer, Maximilian
A1  - Wagner, Patrick
A1  - Schöning, Michael Josef
T1  - Detection of toxic chromium species in water using cellbased sensor systems
Y1  - 2012
SN  - 978-3-9813484-2-2
U6  - https://doi.org/10.5162/IMCS2012/P2.1.14
SP  - 1364
EP  - 1367
ER  - 
TY  - JOUR
A1  - Bertz, Morten
A1  - Schöning, Michael Josef
A1  - Molinnus, Denise
A1  - Homma, Takayuki
T1  - Influence of temperature, light, and H₂O₂ concentration on microbial spore inactivation: in-situ Raman spectroscopy combined with optical trapping
JF  - Physica status solidi (a) applications and materials science
N2  - To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.
KW  - hydrogen peroxide
KW  - optical spore trapping
KW  - Raman spectroscopy
KW  - sterilization conditions
KW  - temperature
Y1  - 2024
U6  - https://doi.org/10.1002/pssa.202300866
SN  - 1862-6319 (Online)
SN  - 1862-6300 (Print)
N1  - Corresponding author: Michael J. Schöning
IS  - Early View
PB  - Wiley-VCH
CY  - Berlin
ER  -