TY  - JOUR
A1  - Burger, René
A1  - Lindner, Simon
A1  - Rumpf, Jessica
A1  - Do, Xuan Tung
A1  - Diehl, Bernd W.K.
A1  - Rehahn, Matthias
A1  - Monakhova, Yulia
A1  - Schulze, Margit
T1  - Benchtop versus high field NMR: Comparable performance found for the molecular weight determination of lignin
JF  - Journal of Pharmaceutical and Biomedical Analysis
N2  - Lignin is a promising renewable biopolymer being investigated worldwide as an environmentally benign substitute of fossil-based aromatic compounds, e.g. for the use as an excipient with antioxidant and antimicrobial properties in drug delivery or even as active compound. For its successful implementation into process streams, a quick, easy, and reliable method is needed for its molecular weight determination. Here we present a method using 1H spectra of benchtop as well as conventional NMR systems in combination with multivariate data analysis, to determine lignin’s molecular weight (Mw and Mn) and polydispersity index (PDI). A set of 36 organosolv lignin samples (from Miscanthus x giganteus, Paulownia tomentosa and Silphium perfoliatum) was used for the calibration and cross validation, and 17 samples were used as external validation set. Validation errors between 5.6% and 12.9% were achieved for all parameters on all NMR devices (43, 60, 500 and 600 MHz). Surprisingly, no significant difference in the performance of the benchtop and high-field devices was found. This facilitates the application of this method for determining lignin’s molecular weight in an industrial environment because of the low maintenance expenditure, small footprint, ruggedness, and low cost of permanent magnet benchtop NMR systems.
KW  - NMR
KW  - PLS-regression
KW  - Molecular weight determination
KW  - Chemometrics
KW  - Biomass
Y1  - 2022
SN  - 0731-7085
U6  - http://dx.doi.org/10.1016/j.jpba.2022.114649
VL  - 212
IS  - Article number: 114649
PB  - Elsevier
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Strobel, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Hydrogels with incorporated graphene oxide as light-addressable actuator materials for cell culture environments in lab-on-chip systems
JF  - Physica status solidi (a)
N2  - Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.
Y1  - 2016
U6  - http://dx.doi.org/10.1002/pssa.201533056
SN  - 1862-6300
VL  - 213
IS  - 6
SP  - 1520
EP  - 1525
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, Ronald
A1  - Wagner, Torsten
T1  - Light-stimulated hydrogel actuators with incorporated graphene oxide for microfluidic applications
T2  - 12. Dresdner Sensor-Symposium 2015
Y1  - 2015
U6  - http://dx.doi.org/10.5162/12dss2015/P5.8
SP  - 206
EP  - 209
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Kirschbaum, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide
JF  - Physica status solidi (a)
N2  - Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431944
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1368
EP  - 1374
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, Ronald
A1  - Wagner, Torsten
T1  - Investigation of the spatial resolution of a laser-based stimulation process for light-addressable hydrogels with incorporated graphene oxide by means of IR thermography
JF  - Sensors and Actuators A: Physical
Y1  - 2017
U6  - http://dx.doi.org/10.1016/j.sna.2017.11.031
SN  - 0924-4247
VL  - 268
SP  - 126
EP  - 132
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Brandini, Frederico P.
A1  - Baumann, Marcus
T1  - The potential role of melted 'brown ice' as sources of chelators and ammonia to the surface waters of the Weddell Sea, Antarctica
T2  - Proceedings of the NIPR Symposium on Polar Biology : 10
Y1  - 1997
SN  - 0914-563X
SP  - 1
EP  - 13
CY  - Tokyo
ER  - 
TY  - JOUR
A1  - Braband, Henrik
A1  - Yegen, Eda
A1  - Paulßen, Elisabeth
A1  - Abram, Ulrich
T1  - [{ReN(PMe2Ph)3}{ReO3N}]2 – Structural Evidence for the Nitridotrioxorhenate(VII) Anion, [ReO3N]2−
JF  - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry
Y1  - 2005
U6  - http://dx.doi.org/10.1002/zaac.200500240
SN  - 1521-3749
VL  - 631
IS  - 12
SP  - 2408
EP  - 2410
ER  - 
TY  - JOUR
A1  - Braband, Henrik
A1  - Paulßen, Elisabeth
A1  - Abram, Ulrich
T1  - Nitridorhenium(V) Complexes with 1,3-Dialkyl-4,5-dimethylimidazole-2-ylidenes
JF  - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry
Y1  - 2006
U6  - http://dx.doi.org/10.1002/zaac.200600002
SN  - 1521-3749
VL  - 632
IS  - 6
SP  - 1051
EP  - 1056
ER  - 
TY  - JOUR
A1  - Bouwman, Peter
A1  - Gulden, Hanneke van der
A1  - Heijden, Ingrid van der
A1  - Drost, Rinske
A1  - Klijn, Christiaan N.
A1  - Prasetyanti, Pramudita
A1  - Pieterse, Mark
A1  - Wientjens, Ellen
A1  - Seibler, Jost
A1  - Hogervorst, Frank B. L.
A1  - Jonkers, Jos
T1  - A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants
JF  - Cancer Discovery
Y1  - 2013
U6  - http://dx.doi.org/10.1158/2159-8290.CD-13-0094
SN  - 2159-8290
IS  - 3
SP  - 1142
EP  - 1152
ER  - 
TY  - JOUR
A1  - Borgmeier, Claudia
A1  - Bongaerts, Johannes
A1  - Meinhardt, Friedhelm
T1  - Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production
JF  - Journal of biotechnology
N2  - Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele – known to cause hypersecretion in Bacillus subtilis – resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG – known to interfere with DegU DNA-binding in B. subtilis – did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.
KW  - Marker-free mutagenesis
KW  - Extracellular enzymes
KW  - Uracil-phosphoribosyltransferase
KW  - Hypersecretion
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiotec.2012.02.011
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 159
IS  - 1-2
SP  - 12
EP  - 20
PB  - Elsevier
CY  - Amsterdam
ER  -