TY  - JOUR
A1  - Oosterhuis, Koen
A1  - Öhlschläger, Peter
A1  - Berg, Joost H. van den
A1  - Toebes, Mireille
A1  - Gomez, Raquel
A1  - Schumacher, Ton N.
A1  - Haanen, John B.
T1  - Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7
JF  - International Journal of Cancer
Y1  - 2011
SN  - 1097-0215
VL  - 129
IS  - 2
SP  - 397
EP  - 406
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Henken, F. E.
A1  - Oosterhuis, K.
A1  - Öhlschläger, Peter
A1  - Bosch, L.
A1  - Hooijberg, E.
A1  - Haanen, J. B. A. G.
A1  - Steenbergen, R. D. M.
T1  - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7
JF  - Vaccine
N2  - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.vaccine.2012.04.013
SN  - 0264-410X
VL  - 30
IS  - 28
SP  - 4259
EP  - 4266
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Schmid, Georg
A1  - Wandrey, Christian
T1  - Preparation of cellodextrins and isolation of oligomeric side components and their characterization / Schmid, Georg ; Biselli, Manfred ; Wandrey, Christian
JF  - Analytical Biochemistry. 175 (1988)
Y1  - 1988
SN  - 0003-2697
SP  - 573
EP  - 583
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Hammon, U.
T1  - Preparation of pellets with well-defined pore structure / U. Hammon ; A. Kotter
JF  - International chemical engineering. 26 (1986), H. 4
Y1  - 1986
SN  - 0020-6318
SP  - 563
EP  - 573
ER  - 
TY  - JOUR
A1  - Naithani, V. K
A1  - Klostermeyer, Henning
A1  - Lange, H. R.
A1  - [u.a.], 
A1  - Berndt, Heinz
A1  - [u.a.], 
T1  - Preparation of peptide derivatives for porcine proinsulin-synthesis
JF  - Biological Chemistry
Y1  - 1971
U6  - http://dx.doi.org/10.1515/bchm2.1971.352.1.1
SN  - 1437-4315
VL  - 352
IS  - 1
SP  - 2
EP  - 3
PB  - De Gruyter
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Woznyj, M.
A1  - Lüdemann, H.-D.
T1  - Pressure Dependence of the Melting and Self Diffusion in 2,2-Dimethylpropane, 2,2-Dimethylpropionitrile, and 2-Methylpropanol-2 / M. Woznyj, F. X. Prielmeier, H.-D. Lüdemann
JF  - Zeitschrift für Naturforschung A, Journal of Physical Sciences. 39 (1984)
Y1  - 1984
SN  - 0932-0784
SP  - 800
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Lang, E. W.
A1  - Lüdemann, H.-D.
T1  - Pressure dependence of the self-diffusion in liquid trifluoromethane / F. X. Prielmeier; E. W. Lang; H.-D. Lüdemann
JF  - Molecular Physics. 52 (1984), H. 5
Y1  - 1984
SN  - 0026-8976
SP  - 1105
EP  - 1113
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Riekert, Lothar
A1  - Simoncic, V.
T1  - Pristup sintezi industrijskih katalizatora
JF  - Kemija u industriji : casopis kemicara i tehnologa Jugoslavije. 35 (1986)
Y1  - 1986
SN  - 0022-9830
N1  - Hrsg.: Croatian Society of Chemical Engineers
SP  - 79
EP  - 86
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Muratyan, Simone
T1  - Problematik verkehrsbezogener Außenluftmessungen von Partikeln (Dieselruß)
JF  - + Krebserzeugende Stoffe in der Umwelt : Herkunft, Messung, Risiko, Minimierung ; Kolloquium Mannheim, 23. bis 25. April 1991 / Kommission Reinhaltung der Luft im VDI und DIN
Y1  - 1991
SN  - 3-18-090888-2
N1  - VDI-Berichte ; 888
SP  - 143
PB  - VDI-Verl.
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements
JF  - Geophysical research letters
Y1  - 1994
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 21
IS  - Iss. 8
SP  - 689
EP  - 692
ER  -