TY - BOOK A1 - Appel, Wolfgang A1 - Brähler, Hermann A1 - Dahlhaus, Ulrich A1 - Esch, Thomas A1 - Kopp, Stephan A1 - Rhein, Bernd T1 - Nutzfahrzeugtechnik : Grundlagen, Systeme, Komponenten. 6., überarb. Aufl. Y1 - 2010 SN - 978-3-8348-9757-2 PB - Vieweg + Teubner CY - Wiesbaden ER - TY - JOUR A1 - Ahmed, Essam A1 - Reisgen, Uwe A1 - Schleser, Markus A1 - Mokrov, Oleg T1 - On formability of tailor laser welded blanks of DP/TRIP steel sheets JF - Science and technology of welding and joining N2 - This paper aims to evaluate the formability of tailor welded blanks of dual phase (DP600)/transformation induced plasticity (TRIP700) steel sheets. In this work, bead on plate butt joints of 2·5 mm DP600 and 1·2 mm TRIP700 steel sheets were performed using CO2 laser beam welding. Microhardness measurements and transverse tensile testing were carried out to characterise the welds. The formability of base metals and welds were investigated by standard Erichsen test. In a perpendicular tensile test to the weld line, all specimens were fractured at the TRIP base metal, and the strengths were somewhat higher than those of base metal. There was a significant reduction in formability caused by welding of the DP600/TRIP700 steel sheets, and the formability increased with increasing welding speed. Y1 - 2010 U6 - http://dx.doi.org/10.1179/136217110X12731414739754 SN - 1743-2936 VL - 15 IS - 5 SP - 337 EP - 342 PB - Maney CY - Leeds ER - TY - JOUR A1 - Ferrein, Alexander A1 - Steinbauer, Gerald T1 - On the Way to High-Level Programming for Resource-Limited Embedded Systems with Golog JF - Simulation, Modeling, and Programming for Autonomous Robots Y1 - 2010 N1 - Lecture Notes in Computer Science ; 6472 ; Second International Conference, SIMPAR 2010, Darmstadt, Germany, November 15-18, 2010. Proceedings SP - 229 EP - 240 ER - TY - JOUR A1 - Srivastava, A. A1 - Singh, V. A1 - Aggarwal, P. A1 - Schneeweiss, F. A1 - Scherer, Ulrich W. A1 - Friedrich, W. T1 - Optical studies of insulating polymers for radiation dose monitoring JF - Indian Journal of Pure & Applied Physics Y1 - 2010 SN - 0019-5596 N1 - Special Issue: SI VL - 48 IS - 11 SP - 782 EP - 786 ER - TY - JOUR A1 - Kern, Alexander A1 - Klöters, Georg A1 - Pferdemenges, Jörg T1 - Optimised protection against lightning for the signalling and safety systems of level crossings on non-electrified railway lines JF - RTR : European Rail Technology Review Y1 - 2010 SN - 0079-9548 VL - 50 IS - 2 ER - TY - JOUR A1 - Degering, Christian A1 - Eggert, Thorsten A1 - Puls, Michael A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Jaeger, Karl-Erich T1 - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides JF - Applied and environmental microbiology N2 - Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains. Y1 - 2010 U6 - http://dx.doi.org/10.1128/AEM.01146-10 SN - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print) VL - 76 IS - 19 SP - 6370 EP - 6378 PB - American Society for Microbiology CY - Washington, DC ER - TY - GEN A1 - Alt, Helmut T1 - Optionen einer bezahlbaren Energieversorgung „Vision versus Realismus" : Beitrag für KTG Kongress: vom 4. bis 6. Mai 2010, Berlin Y1 - 2010 SP - 1 EP - 10 ER - TY - BOOK A1 - Siedenbiedel, Georg T1 - Organisation Y1 - 2010 SN - 978-3-8252-8413-8 N1 - UTB ; 8413 : Betriebswirtschaftslehre PB - Lucius & Lucius CY - Stuttgart ER - TY - BOOK A1 - Schulte-Zurhausen, Manfred T1 - Organisation / 5., überarb. und aktualisierte Aufl. Y1 - 2010 SN - 978-3-8006-3736-2 PB - Vahlen CY - München ER - TY - JOUR A1 - Tippkötter, Nils A1 - Roikaew, Wipa A1 - Ulber, Roland A1 - Hoffmann, Alexander A1 - Denzler, Hans-Jörg A1 - Buchholz, Heinrich T1 - Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food JF - Biotechnology Progress N2 - Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed. Y1 - 2010 U6 - http://dx.doi.org/10.1002/btpr.384 SN - 8756-7938 VL - 26 IS - 3 SP - 756 EP - 762 PB - Wiley CY - Hoboken, NJ ER -