TY  - JOUR
A1  - Jahnke, Siegfried
A1  - Roussel, Johanna
A1  - Hombach, Thomas
A1  - Kochs, Johannes
A1  - Fischbach, Andreas
A1  - Huber, Gregor
A1  - Scharr, Hanno
T1  - phenoSeeder - A robot system for automated handling and phenotyping of individual seeds
JF  - Plant physiology
N2  - The enormous diversity of seed traits is an intriguing feature and critical for the overwhelming success of higher plants. In particular, seed mass is generally regarded to be key for seedling development but is mostly approximated by using scanning methods delivering only two-dimensional data, often termed seed size. However, three-dimensional traits, such as the volume or mass of single seeds, are very rarely determined in routine measurements. Here, we introduce a device named phenoSeeder, which enables the handling and phenotyping of individual seeds of very different sizes. The system consists of a pick-and-place robot and a modular setup of sensors that can be versatilely extended. Basic biometric traits detected for individual seeds are two-dimensional data from projections, three-dimensional data from volumetric measures, and mass, from which seed density is also calculated. Each seed is tracked by an identifier and, after phenotyping, can be planted, sorted, or individually stored for further evaluation or processing (e.g. in routine seed-to-plant tracking pipelines). By investigating seeds of Arabidopsis (Arabidopsis thaliana), rapeseed (Brassica napus), and barley (Hordeum vulgare), we observed that, even for apparently round-shaped seeds of rapeseed, correlations between the projected area and the mass of seeds were much weaker than between volume and mass. This indicates that simple projections may not deliver good proxies for seed mass. Although throughput is limited, we expect that automated seed phenotyping on a single-seed basis can contribute valuable information for applications in a wide range of wild or crop species, including seed classification, seed sorting, and assessment of seed quality.
Y1  - 2016
U6  - http://dx.doi.org/10.1104/pp.16.01122
SN  - 0032-0889
VL  - 172
IS  - 3
SP  - 1358
EP  - 1370
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Luisier, Raphaëlle
A1  - Lempiäinen, Harri
A1  - Scherbichler, Nina
A1  - Braeuning, Albert
A1  - Geissler, Miriam
A1  - Dubost, Valerie
A1  - Müller, Arne
A1  - Scheer, Nico
A1  - Chibout, Salah-Dine
A1  - Hara, Hisanori
A1  - Picard, Frank
A1  - Theil, Diethilde
A1  - Couttet, Philippe
A1  - Vitobello, Antonio
A1  - Grenet, Olivier
A1  - Grasl-Kraupp, Bettina
A1  - Ellinger-Ziegelbauer, Heidrung
A1  - Thomson, John P.
A1  - Meehan, Richard R.
A1  - Elcombe, Clifford R.
A1  - Henderson, Colin J.
A1  - Wolf, C. Roland
A1  - Schwarz, Michael
A1  - Moulin, Pierre
A1  - Terranova, Remi
A1  - Moggs, Jonathan G.
T1  - Phenobarbital Induces Cell Cycle Transcriptional Responses in Mouse Liver Humanized for Constitutive Androstane and Pregnane X Receptors
JF  - Toxicological Sciences
N2  - The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CARᴷᴼ-PXRᴷᴼ), double humanized CAR and PXR (CARʰ-PXRʰ), and wild-type C57BL/6 mice. Wild-type and CARʰ-PXRʰ mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CARᴷᴼ-PXRᴷᴼ mouse livers and largely reversible in wild-type and CARʰ-PXRʰ mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CARʰ-PXRʰ mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB.
Y1  - 2014
U6  - http://dx.doi.org/https://doi.org/10.1093/toxsci/kfu038
SN  - 1094-2025
VL  - 139
IS  - 2
SP  - 501
EP  - 511
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Stulpe, Werner
A1  - Singer, Matthias
T1  - Phase-Space Representations of General Statistical Physical Theories. Singer, Matthias; Stulpe, Werner
JF  - Journal of Mathematical Physics. 33 (1992), H. 1
Y1  - 1992
SN  - 1089-7658
SP  - 131
EP  - 142
ER  - 
TY  - JOUR
A1  - Miyamoto, Ko-ichiro
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Kanoh, Shin`ichiro
A1  - Schöning, Michael Josef
T1  - Phase-mode operation of FDM-LAPS
JF  - Sensor letters
Y1  - 2011
SN  - 1546-1971
VL  - 9
IS  - 2
SP  - 691
EP  - 694
PB  - American Scientific Publishers
CY  - Stevenson Ranch, Calif.
ER  - 
TY  - JOUR
A1  - Miyamoto, Ko-ichiro
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Kanoh, Shin`ichiro
A1  - Schöning, Michael Josef
T1  - Phase-mode LAPS and its application to chemical imaging
JF  - Sensors and Actuators B: Chemical. 154 (2011), H. 1
Y1  - 2011
SN  - 1873-3077
SP  - 28
EP  - 32
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Fleischhaker, Robert
A1  - Evers, Jörg
T1  - Phase-controlled pulse propagation in media with cross coupling of electric and magnetic probe field component
JF  - Physical Review A - Atomic, molecular, and optical physics
Y1  - 2009
U6  - http://dx.doi.org/10.1103/PHYSREVA.80.063816
SN  - 1050-2947
VL  - 80
IS  - 6
SP  - 063816
ER  - 
TY  - JOUR
A1  - Fleischhaker, Robert
A1  - Evers, Jörg
A1  - Dey, Tarak N.
T1  - Phase modulation induced by cooperative effects in electromagnetically induced transparency
JF  - Physical Review A - Atomic, molecular, and optical physics
N2  - We analyze the influence of dipole-dipole interactions in an electromagnetically induced transparency set up for a density at the onset of cooperative effects. To this end, we include mean-field models for the influence of local-field corrections and radiation trapping into our calculation. We show both analytically and numerically that the polarization contribution to the local field strongly modulates the phase of a weak pulse. We give an intuitive explanation for this local-field-induced phase modulation and demonstrate that it distinctively differs from the nonlinear self-phase-modulation that a strong pulse experiences in a Kerr medium.
Y1  - 2010
U6  - http://dx.doi.org/10.1103/PhysRevA.82.013815
SN  - 1050-2947
VL  - 82
IS  - 1
PB  - AIP Publishing
CY  - Melville, NY
ER  - 
TY  - JOUR
A1  - Buniatyan, Vahe V.
A1  - Abouzar, Maryam H.
A1  - Martirosyan, Norayr W.
A1  - Schubert, Jürgen
A1  - Gevorgian, Spartak
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - pH-sensitive properties of barium strontium titanate (BST) thin films prepared by pulsed laser deposition technique
JF  - Physica Status Solidi (A). 207 (2010), H. 4
Y1  - 2010
SN  - 1862-6300
N1  - Special Issue: Engineering of Functional Interfaces EnFI 2009
SP  - 824
EP  - 830
ER  - 
TY  - JOUR
A1  - Christiaens, P.
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
A1  - Wagner, Torsten
A1  - Bijnens, N.
A1  - Williams, O. A.
A1  - Daenen, M.
A1  - Haenen, K.
A1  - Douthéret, O.
A1  - Haen, J. d´
A1  - Mekhalif, Z.
A1  - Schöning, Michael Josef
A1  - Wagner, P.
T1  - pH sensitivity of nanocrystalline diamond films
JF  - Physica status solidi (A). 204 (2007), H. 9
Y1  - 2007
SN  - 0031-8965
SP  - 2925
EP  - 2930
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Abouzar, Maryam H.
A1  - Poghossian, Arshak
T1  - pH and ion sensitivity of a field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with polyelectrolyte multilayers
JF  - Journal of Solid State Electrochemistry. 13 (2009), H. 1
Y1  - 2009
SN  - 1433-0768
N1  - This manuscript is an invited paper to the special issue on “Solid-state potentiometric sensors” (Guest editor: Johan Bobacka).
SP  - 115
EP  - 122
ER  -