TY - JOUR A1 - Bäcker, Matthias A1 - Poghossian, Arshak A1 - Abouzar, Maryam H. A1 - Wenmackers, Sylvia A1 - Janssens, Stoffel D. A1 - Haenen, Ken A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Capacitive field-effect (bio-)chemical sensors based on nanocrystalline diamond films JF - Diamond Electronics and Bioelectronics — Fundamentals to Applications III, edited by P. Bergonzo, [u.a.] Y1 - 2010 N1 - MRS Proceedings Volume 1203 paper 1203-J17-31 ; Mater. Res. Soc. Sympos. Proc. Vol 1203 (2010) ; Materials Research Society SP - 1 EP - 6 ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Poghossian, Arshak A1 - Siqueira, José R. Jr. A1 - Oliveira, Osvaldo N. Jr. A1 - Moritz, Werner A1 - Schöning, Michael Josef T1 - Capacitive electrolyte-insulator-semiconductor structures functionalised with a polyelectrolyte/enzyme multilayer: New strategy for enhanced field-effect biosensing JF - Physica Status Solidi (A). 207 (2010), H. 4 Y1 - 2010 SN - 1862-6300 N1 - Special Issue: Engineering of Functional Interfaces EnFI 2009 SP - 884 EP - 890 ER - TY - JOUR A1 - Abouzar, Maryam H. A1 - Werner, Moritz A1 - Schöning, Michael Josef A1 - Poghossian, Arshak T1 - Capacitance–voltage and impedance-spectroscopy characteristics of nanoplate EISOI capacitors JF - Physica status solidi (a) : applications and material science. 208 (2011), H. 6 Y1 - 2011 SN - 1862-6319 SP - 1327 EP - 1332 PB - Wiley-VCH CY - Berlin ER - TY - JOUR A1 - Poghossian, Arshak A1 - Abouzar, Maryam H. A1 - Schöning, Michael Josef T1 - Capacitance–voltage and impedance characteristics of field-effect EIS sensors functionalised with polyelectrolyte multilayers JF - IRBM. 29 (2008), H. 2-3 Y1 - 2008 SN - 1959-0318 SP - 149 EP - 154 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Mourzina, Y. G. A1 - Schubert, J. A1 - Zander, W. A1 - Legin, A. A1 - Vlasov, Y. G. A1 - Lüth, H. T1 - Can pulsed laser deposition serve as an advanced technique in fabricating chemical sensors? JF - Sensors and Actuators B. 78 (2001), H. 1-3 Y1 - 2001 SN - 0925-4005 N1 - Selected Papers form Eurosensors XIV SP - 273 EP - 278 ER - TY - JOUR A1 - Schöning, Michael Josef A1 - Mourzina, Y. G. A1 - Schubert, J. A1 - Zander, W. A1 - Legin, A. A1 - Vlasov, Y. G. A1 - Lüth, H. T1 - Can pulsed laser deposition serve as an advanced technique in fabricating chemical sensors? JF - Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...] Y1 - 2000 SN - 87-89935-50-0 N1 - Eurosensors ; (14, 2000, København) ; Eurosensors ; (14 : ; 2000.08.27-30 : ; Copenhagen) ; European Conference on Solid-State Transducers ; (14 : ; 2000.08.27-30 : ; Copenhagen) SP - 615 EP - 618 PB - MIC, Mikroelektronik Centret CY - Lyngby, Denmark ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - http://dx.doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Paczkowski, Sebastian A1 - Weißbecker, Bernhard A1 - Schöning, Michael Josef A1 - Schütz, Stefan T1 - Biosensors on the Basis of Insect Olfaction JF - Insect biotechnology / Andreas Vilcinskas, ed. Y1 - 2011 SN - 978-90-481-9640-1 N1 - Biologically-inspired system ; 2 SP - 225 EP - 240 PB - Springer CY - Dordrecht [u.a.] ER - TY - JOUR A1 - Keusgen, Michael A1 - Jünger, Martina A1 - Krest, Ingo A1 - Schöning, Michael Josef T1 - Biosensoric detection of the cysteine sulphoxide alliin JF - Sensors and Actuators B. 95 (2003), H. 1-3 Y1 - 2003 SN - 0925-4005 SP - 297 EP - 302 ER - TY - JOUR A1 - Keusgen, M. A1 - Jünger, M. A1 - Schöning, Michael Josef T1 - Biosensoric detection of the cysteine sulphoxide alliin JF - Book of abstracts / ed. by J. Saneistr. Y1 - 2002 SN - 80-01-02576-4 N1 - Eurosensors ; (16, 2002, Praha) SP - 1175 EP - 1178 PB - Czech Technical University, Faculty of Electrical Engineering, Department of Measurement CY - Prague ER -