TY  - CHAP
A1  - Kasper, Katharina
A1  - Schiffels, Johannes
A1  - Krafft, Simone
A1  - Kuperjans, Isabel
A1  - Elbers, Gereon
A1  - Selmer, Thorsten
T1  - Biogas Production on Demand Regulated by Butyric Acid Addition
T2  - IOP Conference Series: Earth and Environmental Science. Bd. 32
Y1  - 2016
U6  - http://dx.doi.org/10.1088/1755-1315/32/1/012009
SN  - 1755-1315
N1  - ICARET 2016, International Conference on Advances in Renewable Energy and Technologies, Putrajaya, MY, Feb 23-25, 2016
VL  - 32
SP  - 012009/1
EP  - 012009/4
ER  - 
TY  - JOUR
A1  - Schöning, Michael Josef
A1  - Poghossian, Arshak
T1  - BioFEDs (field-effect devices) : State-of-the-art and new directions
JF  - Electroanalysis
Y1  - 2006
U6  - http://dx.doi.org/10.1002/elan.200603609
SN  - 1521-4109
VL  - 18
IS  - 19-20
SP  - 1893
EP  - 1900
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Weißbecker, B.
A1  - Schütz, S.
A1  - Ecken, H.
A1  - Yoshinobu, T.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Bioelectronic signal processing - intact chemoreceptors coupled to field-effect devices
JF  - Lecture Notes of the ICB Seminars
Y1  - 2002
SP  - 28
EP  - 42
PB  - MCB
CY  - Warsaw
ER  - 
TY  - JOUR
A1  - Schroth, P.
A1  - Weißbecker, B.
A1  - Schütz, S.
A1  - Ecken, H.
A1  - Yoshinobu, T.
A1  - Lüth, H.
A1  - Schöning, Michael Josef
T1  - Bioelectronic signal processing - intact chemoreceptors coupled to field-effect devices
JF  - Biocybernetics and Biomedical Engineering. 21 (2001), H. 3
Y1  - 2001
SN  - 0208-5216
SP  - 27
EP  - 42
ER  - 
TY  - JOUR
A1  - Lüth, H.
A1  - Thust, M.
A1  - Steffen, A.
A1  - Kordos, P.
A1  - Schöning, Michael Josef
T1  - Biochemical sensors with structured and porous silicon capacitors
JF  - Materials Science and Engineering B. 69-70 (2000)
Y1  - 2000
SN  - 0921-5107
SP  - 104
EP  - 108
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Rahba, Jade
A1  - Fischer, David
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T
JF  - FEBS Open Bio
N2  - Halophilic and halotolerant microorganisms represent a promising source of salt-tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market and the need for more efficient and new biocatalysts, the present study aimed at the characterization of a high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T. The protease gene was cloned and expressed in Bacillus subtilis DB104. The recombinant protease SPAO with 269 amino acids belongs to the subfamily of high-alkaline subtilisins. The biochemical characteristics of purified SPAO were analyzed in comparison with subtilisin Carlsberg, Savinase, and BPN'. SPAO, a monomer with a molecular mass of 27.1 kDa, was active over a wide range of pH 6.0–12.0 and temperature 20–80 °C, optimally at pH 9.0–9.5 and 55 °C. The protease is highly oxidatively stable to hydrogen peroxide and retained 58% of residual activity when incubated at 10 °C with 5% (v/v) H2O2 for 1 h while stimulated at 1% (v/v) H2O2. Furthermore, SPAO was very stable and active at NaCl concentrations up to 5.0 m. This study demonstrates the potential of SPAO for biotechnological applications in the future.
KW  - Alkalihalobacillus okhensis
KW  - detergent protease
KW  - halotolerant protease
KW  - high-alkaline subtilisin
KW  - oxidative stable protease
Y1  - 2022
U6  - http://dx.doi.org/10.1002/2211-5463.13457
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 12
IS  - 10
SP  - 1729
EP  - 1746
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Kohn, Sophie
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ
JF  - FEBS Open Bio
N2  - Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications.
KW  - Bacillaceae
KW  - Biotechnological application
KW  - Broad pH spectrum
KW  - Subtilases
KW  - Subtilisin
Y1  - 2023
U6  - http://dx.doi.org/10.1002/2211-5463.13701
SN  - 2211-5463
N1  - Corresponding author: Petra Siegert
VL  - 13
IS  - 11
SP  - 2035
EP  - 2046
PB  - Wiley
CY  - Hoboken, NJ
ER  - 
TY  - JOUR
A1  - Kramer, Friederike
A1  - Halamkova, Lenka
A1  - Poghossian, Arshak
A1  - Schöning, Michael Josef
A1  - Katz, Evgeny
A1  - Halamek, Jan
T1  - Biocatalytic analysis of biomarkers for forensic identification of ethnicity between Caucasian and African American
JF  - The analyst. August 2013
Y1  - 2013
SN  - 1364-5528 (E-Journal); 0003-2654 (Print)
VL  - Vol. 138
SP  - 6251
EP  - 6257
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Groebel, Simone
A1  - Schuhmacher, K.
A1  - Spelthahn, Heiko
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Bestimmung der metabolischen Aktivität von Mikroorganismen während des Biogasbildungsprozesses
JF  - 9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]
Y1  - 2009
SN  - 978-3-941298-44-6
N1  - Dresdner Sensor-Symposium ; (9, 2009, Dresden)
SP  - 201
EP  - 204
PB  - TUDpress
CY  - Dresden
ER  - 
TY  - JOUR
A1  - Darmo, J.
A1  - Schäffer, F.
A1  - Förster, Arnold
A1  - Kordos, P.
T1  - Beryllium doped low-temperature-grown MBE GaAs: material for photomixing in the THz frequency range
JF  - ASDAM 2000 : conference proceedings / edited by Jozef Osvald ... [et al.]
Y1  - 2000
SN  - 0780359399
N1  - International Conference on Advanced Semiconductor Devices and Microsystems ; (3rd : ; 2000 : ; Smolenice, Slovakia)
SP  - 147
EP  - 150
PB  - IEEE
CY  - Piscataway, NJ
ER  -