TY - JOUR A1 - Vögele, Stefan A1 - Rübbelke, Dirk A1 - Govorukha, Kristina A1 - Grajewski, Matthias T1 - Socio-technical scenarios for energy-intensive industries: the future of steel production in Germany JF - Climatic Change Y1 - 2019 U6 - https://doi.org/10.1007/s10584-019-02366-0 SN - 0165-0009 SP - 1 EP - 16 PB - Springer CY - Berlin ER - TY - CHAP A1 - Bertsch, Timo A1 - Meinecke, Matthias A1 - Wolf, Martin R. A1 - Schmunk, Karina T1 - Smart-Living-Services nur gegen Daten? Process-Mining als Möglichkeit zur Steigerung der Akzeptanz! T2 - Angewandte Forschung in der Wirtschaftsinformatik 2019 : Tagungsband zur 32. AKWI-Jahrestagung / hrsg. von Martin R. Wolf, Thomas Barton, Frank Herrmann, Vera G. Meister, Christian Müller, Christian Seel N2 - Seit Jahren etablieren sich Technologien in unserem Alltag, die mit Hilfe von smarten Komponenten neue Services und Vernetzungsmöglichkeiten schaffen. Dieses Paper beschreibt die Ergebnisse einer Studie, die die Akzeptanz von IoT-gestützten, smarten Services im privaten Umfeld untersucht. Dabei wird eine zentrale Datenverarbeitung mit automatisierter Erstellung smarter Services der dezentralen Datenverarbeitung mit manueller Serviceerstellung in sieben Kategorien gegenübergestellt. Die Auswertung der Studie legt die Forschungsfrage nahe, ob das Nutzerverhalten im Kontext Smart Living nicht auch mit einem dezentralen Lösungsansatz, und somit unabhängig von großen Unternehmen, analysiert werden kann. Hierfür wird im zweiten Teil des Papers die Anwendbarkeit von Process-Mining im Bereich Smart Living untersucht und prototypisch getestet. Y1 - 2019 SN - 978-3-944330-62-4 SP - 216 EP - 226 PB - mana-Buch CY - Heide ER - TY - JOUR A1 - Thoma, Andreas A1 - Ravi, Sridhar T1 - Significance of parallel computing on the performance of Digital Image Correlation algorithms in MATLAB N2 - Digital Image Correlation (DIC) is a powerful tool used to evaluate displacements and deformations in a non-intrusive manner. By comparing two images, one of the undeformed reference state of a specimen and another of the deformed target state, the relative displacement between those two states is determined. DIC is well known and often used for post-processing analysis of in-plane displacements and deformation of specimen. Increasing the analysis speed to enable real-time DIC analysis will be beneficial and extend the field of use of this technique. Here we tested several combinations of the most common DIC methods in combination with different parallelization approaches in MATLAB and evaluated their performance to determine whether real-time analysis is possible with these methods. To reflect improvements in computing technology different hardware settings were also analysed. We found that implementation problems can reduce the efficiency of a theoretically superior algorithm such that it becomes practically slower than a suboptimal algorithm. The Newton-Raphson algorithm in combination with a modified Particle Swarm algorithm in parallel image computation was found to be most effective. This is contrary to theory, suggesting that the inverse-compositional Gauss-Newton algorithm is superior. As expected, the Brute Force Search algorithm is the least effective method. We also found that the correct choice of parallelization tasks is crucial to achieve improvements in computing speed. A poorly chosen parallelisation approach with high parallel overhead leads to inferior performance. Finally, irrespective of the computing mode the correct choice of combinations of integerpixel and sub-pixel search algorithms is decisive for an efficient analysis. Using currently available hardware realtime analysis at high framerates remains an aspiration. Y1 - 2019 SP - 1 EP - 17 ER - TY - CHAP A1 - Rings, René A1 - Ludowicy, Jonas A1 - Finger, Felix A1 - Braun, Carsten A1 - Bil, Cees T1 - Sensitivity Analysis of General Aviation Aircraft with Parallel Hybrid-Electric Propulsion Systems T2 - Asia Pacific International Symposium on Aerospace Technology. APISAT 2019 Y1 - 2019 ER - TY - JOUR A1 - Cornelis, Peter A1 - Givanoudi, Stella A1 - Yongabi, Derick A1 - Iken, Heiko A1 - Duwé, Sam A1 - Deschaume, Olivier A1 - Robbens, Johan A1 - Dedecker, Peter A1 - Bartic, Carmen A1 - Wübbenhorst, Michael A1 - Schöning, Michael Josef A1 - Heyndrickx, Marc A1 - Wagner, Patrick T1 - Sensitive and specific detection of E. coli using biomimetic receptors in combination with a modified heat-transfer method JF - Biosensors and Bioelectronics Y1 - 2019 U6 - https://doi.org/10.1016/j.bios.2019.04.026 SN - 0956-5663 VL - 136 SP - 97 EP - 105 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Achtsnicht, Stefan A1 - Neuendorf, Christian A1 - Faßbender, Tobias A1 - Nölke, Greta A1 - Offenhäusser, Andreas A1 - Krause, Hans-Joachim A1 - Schröper, Florian T1 - Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection JF - Plos One N2 - Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more than 700 ng/ml. Y1 - 2019 U6 - https://doi.org/10.1371/journal.pone.0219356 SN - 1932-6203 VL - 14 IS - 7 PB - Plos CY - San Francisco ER - TY - CHAP A1 - Butenweg, Christoph A1 - Holtschoppen, Britta T1 - Seismic design of structures and components in industrial units T2 - Structural Dynamics with Applications in Earthquake and Wind Engineering N2 - Industrial units consist of the primary load-carrying structure and various process engineering components, the latter being by far the most important in financial terms. In addition, supply structures such as free-standing tanks and silos are usually required for each plant to ensure the supply of material and product storage. Thus, for the earthquake-proof design of industrial plants, design and construction rules are required for the primary structures, the secondary structures and the supply structures. Within the framework of these rules, possible interactions of primary and secondary structures must also be taken into account. Importance factors are used in seismic design in order to take into account the usually higher risk potential of an industrial unit compared to conventional building structures. Industrial facilities must be able to withstand seismic actions because of possibly wide-ranging damage consequences in addition to losses due to production standstill and the destruction of valuable equipment. The chapter presents an integrated concept for the seismic design of industrial units based on current seismic standards and the latest research results. Special attention is devoted to the seismic design of steel thin-walled silos and tank structures. KW - Industrial units KW - Seismic design KW - Tanks KW - Silos KW - Components Y1 - 2019 SN - 978-3-662-57550-5 U6 - https://doi.org/10.1007/978-3-662-57550-5_5 SP - 359 EP - 481 PB - Springer CY - Berlin ER - TY - JOUR A1 - Knödler, Matthias A1 - Rühl, Clemens A1 - Emonts, Jessica A1 - Buyel, Johannes Felix T1 - Seasonal weather changes affect the yield and quality of recombinant proteins produced in transgenic tobacco plants in a greenhouse setting JF - Frontiers in Plant Science N2 - Transgenic plants have the potential to produce recombinant proteins on an agricultural scale, with yields of several tons per year. The cost-effectiveness of transgenic plants increases if simple cultivation facilities such as greenhouses can be used for production. In such a setting, we expressed a novel affinity ligand based on the fluorescent protein DsRed, which we used as a carrier for the linear epitope ELDKWA from the HIV-neutralizing antibody 2F5. The DsRed-2F5-epitope (DFE) fusion protein was produced in 12 consecutive batches of transgenic tobacco (Nicotiana tabacum) plants over the course of 2 years and was purified using a combination of blanching and immobilized metal-ion affinity chromatography (IMAC). The average purity after IMAC was 57 ± 26% (n = 24) in terms of total soluble protein, but the average yield of pure DFE (12 mg kg−1) showed substantial variation (± 97 mg kg−1, n = 24) which correlated with seasonal changes. Specifically, we found that temperature peaks (>28°C) and intense illuminance (>45 klx h−1) were associated with lower DFE yields after purification, reflecting the loss of the epitope-containing C-terminus in up to 90% of the product. Whereas the weather factors were of limited use to predict product yields of individual harvests conducted for each batch (spaced by 1 week), the average batch yields were well approximated by simple linear regression models using two independent variables for prediction (illuminance and plant age). Interestingly, accumulation levels determined by fluorescence analysis were not affected by weather conditions but positively correlated with plant age, suggesting that the product was still expressed at high levels, but the extreme conditions affected its stability, albeit still preserving the fluorophore function. The efficient production of intact recombinant proteins in plants may therefore require adequate climate control and shading in greenhouses or even cultivation in fully controlled indoor farms. KW - batch reproducibility KW - environmental correlation KW - fluorescent protein carrier KW - greenhouse cultivation KW - plant molecular farming Y1 - 2019 U6 - https://doi.org/10.3389/fpls.2019.01245 SN - 1664-462X (online-ressource) IS - 10 PB - Frontiers Media CY - Lausanne ER - TY - JOUR A1 - Pilas, Johanna A1 - Selmer, Thorsten A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Screen-printed carbon electrodes modified with graphene oxide for the design of a reagent-free NAD+-dependent biosensor array JF - Analytical Chemistry Y1 - 2019 U6 - https://doi.org/10.1021/acs.analchem.9b04481 VL - 91 IS - 23 SP - 15293 EP - 15299 PB - ACS Publications CY - Washington ER - TY - PAT A1 - Hojdis, Nils A1 - Multhaupt, Hendrik A1 - Recker, Carla A1 - Wark, Michael T1 - Schwefelvernetzbare Kautschukmischung, Vulkanisat der Kautschukmischung und Fahrzeugreifen N2 - Die Erfindung betrifft eine schwefelvernetzbare Kautschukmischung, deren Vulkanisat und einen Fahrzeugreifen. Die erfindungsgemäße Kautschukmischung enthält wenigstens folgende Bestandteile: - Wenigstens einen Dienkautschuk; - wenigstens eine Kohle (HTC-Kohle), die mittels hydrothermaler Karbonisierung von wenigstens einer Ausgangssubstanz hergestellt ist. Der erfindungsgemäße Fahrzeugreifen weist in wenigstens einem Bauteil wenigstens ein erfindungsgemäßes Vulkanisat der Kautschukmischung auf. Y1 - 2019 N1 - Patent EP3470457A1 17.04.2019 auch veröffentlicht unter WO2019072431A1 ER -