TY  - JOUR
A1  - Biselli, Manfred
A1  - Meissner, Petra
A1  - Schröder, Bernd
A1  - Herfurth, Cornelia
T1  - Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells / Meissner, Petra ; Schröder, Bernd ; Herfurth, Cornelia ; Biselli, Manfred
JF  - Cytotechnology. 30 (1999), H. 1
Y1  - 1999
SN  - 0920-9069
SP  - 227
EP  - 234
ER  - 
TY  - JOUR
A1  - Dünkelmann, Pascal
A1  - Kolter-Jung, Doris
A1  - Nitsche, Adam
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Lingen, Bettina
A1  - Baumann, Martin
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation
JF  - Journal of the American Chemical Society
Y1  - 2002
SN  - 1520-5126 (E-Journal); 0002-7863 (Print)
VL  - Vol. 124
SP  - 12084
EP  - 12085
ER  - 
TY  - JOUR
A1  - Seifarth, Volker
A1  - Goßmann, Matthias
A1  - Grosse, J. O.
A1  - Becker, C.
A1  - Heschel, I.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds
JF  - Urologia Internationalis
Y1  - 2015
U6  - https://doi.org/10.1159/000368419
SN  - 0042-1138
VL  - 2015
IS  - 95
SP  - 106
EP  - 113
PB  - Karger
CY  - Basel
ER  - 
TY  - JOUR
A1  - Turek, Monika
A1  - Ketterer, Lothar
A1  - Claßen, Melanie
A1  - Berndt, Heinz
A1  - Elbers, Gereon
A1  - Krüger, Peter
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development and Electrochemical Investigations of an EIS-(Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection
JF  - Sensors
Y1  - 2007
SN  - 1424-8220
VL  - 7
IS  - 8
SP  - 1415
EP  - 1426
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Muschallik, Lukas
A1  - Gonzalez, Laura Osorio
A1  - Bongaerts, Johannes
A1  - Wagner, Torsten
A1  - Selmer, Thorsten
A1  - Siegert, Petra
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development and characterization of a field-effect biosensor for the detection of acetoin
JF  - Biosensors and Bioelectronics
N2  - A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO₂/Ta₂O₅/enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T, has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied. Typical biosensor parameters such as optimal pH working range, sensitivity, hysteresis, linear concentration range and long-term stability have been examined by means of constant-capacitance (ConCap) mode measurements. Furthermore, preliminary experiments have been successfully carried out for the detection of acetoin in diluted white wine samples.
Y1  - 2018
U6  - https://doi.org/10.1016/j.bios.2018.05.023
VL  - 115
SP  - 1
EP  - 6
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Küppers, Tobias
A1  - Steffen, Victoria
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Wiechert, Wolfgang
T1  - Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
JF  - Microbial cell factories
Y1  - 2014
U6  - https://doi.org/10.1186/1475-2859-13-46
SN  - 1475-2859 (E-Journal)
VL  - 13
SP  - Article No. 46
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Türler, A.
A1  - Gäggeler, H. W.
A1  - Jost, D. T.
T1  - Determination of the Partial Electron-Capture- and Spontaneous-Fission Half-Lives of 254No / A. Türler, H.W. Gäggeler, D.T. Jost, P. Armbruster, W. Brüchle, H. Folger, F.P. Heßberger,  S. Hofmann,
JF  - Zeitschrift für Physik A Hadrons and Nuclei. 331 (1988), H. 3
Y1  - 1988
SN  - 0939-7922
SP  - 363
EP  - 364
ER  - 
TY  - JOUR
A1  - Werner, Frederik
A1  - Krumbe, Christoph
A1  - Schumacher, Katharina
A1  - Groebel, Simone
A1  - Spelthahn, Heiko
A1  - Stellberg, Michael
A1  - Wagner, Torsten
A1  - Yoshinobu, Tatsuo
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Baumann, Marcus
A1  - Schöning, Michael Josef
T1  - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor
JF  - Physica status solidi (a) : applications and material science. 208 (2011), H. 6
Y1  - 2011
SN  - 1862-6319
SP  - 1340
EP  - 1344
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Deterding, A.
A1  - Ulber, Roland
T1  - Determination of acetic acid in fermentation broth by gas-diffusion technique
JF  - Engineering in Life Sciences
N2  - Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5–5.0 g/L acetic acid with a relative standard deviation of <5 % was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.
Y1  - 2008
U6  - https://doi.org/10.1002/elsc.200820227
VL  - 8
IS  - 1, Special Issue: Technical Systems for the Use in Life Sciences
SP  - 62
EP  - 67
ER  - 
TY  - CHAP
A1  - Takenaga, Shoko
A1  - Herrera, Cony F.
A1  - Werner, Frederik
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Öhlschläger, Peter
A1  - Wagner, Torsten
T1  - Detection of the metabolic activity of cells by differential measurements based on a single light-addressable potentiometric sensor chip
T2  - 11. Dresdner Sensor-Symposium : 9.-11.12.2013
Y1  - 2013
SN  - 978-3-9813484-5-3
SP  - 63
EP  - 67
ER  -