TY - JOUR A1 - Clausnitzer, Julian A1 - Kleefeld, Andreas T1 - A spectral Galerkin exponential Euler time-stepping scheme for parabolic SPDEs on two-dimensional domains with a C² boundary JF - Discrete and Continuous Dynamical Systems - Series B N2 - We consider the numerical approximation of second-order semi-linear parabolic stochastic partial differential equations interpreted in the mild sense which we solve on general two-dimensional domains with a C² boundary with homogeneous Dirichlet boundary conditions. The equations are driven by Gaussian additive noise, and several Lipschitz-like conditions are imposed on the nonlinear function. We discretize in space with a spectral Galerkin method and in time using an explicit Euler-like scheme. For irregular shapes, the necessary Dirichlet eigenvalues and eigenfunctions are obtained from a boundary integral equation method. This yields a nonlinear eigenvalue problem, which is discretized using a boundary element collocation method and is solved with the Beyn contour integral algorithm. We present an error analysis as well as numerical results on an exemplary asymmetric shape, and point out limitations of the approach. KW - Nonlinear eigenvalue problems KW - Boundary integral equations, KW - Exponential Euler scheme, KW - Parabolic SPDEs Y1 - 2024 U6 - https://doi.org/10.3934/dcdsb.2023148 SN - 1531-3492 SN - 1553-524X (eISSN) VL - 29 IS - 4 SP - 1624 EP - 1651 PB - AIMS CY - Springfield ER - TY - JOUR A1 - Ayala, Rafael Ceja A1 - Harris, Isaac A1 - Kleefeld, Andreas T1 - Direct sampling method via Landweber iteration for an absorbing scatterer with a conductive boundary JF - Inverse Problems and Imaging N2 - In this paper, we consider the inverse shape problem of recovering isotropic scatterers with a conductive boundary condition. Here, we assume that the measured far-field data is known at a fixed wave number. Motivated by recent work, we study a new direct sampling indicator based on the Landweber iteration and the factorization method. Therefore, we prove the connection between these reconstruction methods. The method studied here falls under the category of qualitative reconstruction methods where an imaging function is used to recover the absorbing scatterer. We prove stability of our new imaging function as well as derive a discrepancy principle for recovering the regularization parameter. The theoretical results are verified with numerical examples to show how the reconstruction performs by the new Landweber direct sampling method. Y1 - 2024 U6 - https://doi.org/10.3934/ipi.2023051 SN - 1930-8337 SN - 1930-8345 (eISSN) VL - 18 IS - 3 SP - 708 EP - 729 PB - AIMS CY - Springfield ER - TY - JOUR A1 - Haeger, Gerrit A1 - Jolmes, Tristan A1 - Oyen, Sven A1 - Jaeger, Karl-Erich A1 - Bongaerts, Johannes A1 - Schörken, Ulrich A1 - Siegert, Petra T1 - Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis JF - Applied Microbiology and Biotechnology N2 - N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC–MS and NMR. KW - Chaperone KW - Biocatalysis KW - Aminoacylase KW - Acylation KW - Acyl-amino acids KW - Biosurfactants Y1 - 2024 U6 - https://doi.org/10.1007/s00253-023-12868-8 SN - 1432-0614 N1 - Corresponding author: Petra Siegert IS - 108 PB - Springer CY - Berlin ER - TY - JOUR A1 - Tix, Julian A1 - Moll, Fabian A1 - Krafft, Simone A1 - Betsch, Matthias A1 - Tippkötter, Nils T1 - Hydrogen production from enzymatic pretreated organic waste with thermotoga neapolitana JF - Energies N2 - Biomass from various types of organic waste was tested for possible use in hydrogen production. The composition consisted of lignified samples, green waste, and kitchen scraps such as fruit and vegetable peels and leftover food. For this purpose, the enzymatic pretreatment of organic waste with a combination of five different hydrolytic enzymes (cellulase, amylase, glucoamylase, pectinase and xylase) was investigated to determine its ability to produce hydrogen (H2) with the hydrolyzate produced here. In course, the anaerobic rod-shaped bacterium T. neapolitana was used for H2 production. First, the enzymes were investigated using different substrates in preliminary experiments. Subsequently, hydrolyses were carried out using different types of organic waste. In the hydrolysis carried out here for 48 h, an increase in glucose concentration of 481% was measured for waste loads containing starch, corresponding to a glucose concentration at the end of hydrolysis of 7.5 g·L−1. In the subsequent set fermentation in serum bottles, a H2 yield of 1.26 mmol H2 was obtained in the overhead space when Terrific Broth Medium with glucose and yeast extract (TBGY medium) was used. When hydrolyzed organic waste was used, even a H2 yield of 1.37 mmol could be achieved in the overhead space. In addition, a dedicated reactor system for the anaerobic fermentation of T. neapolitana to produce H2 was developed. The bioreactor developed here can ferment anaerobically with a very low loss of produced gas. Here, after 24 h, a hydrogen concentration of 83% could be measured in the overhead space. KW - Biological hydrogen KW - Organic waste KW - Dark fermentation KW - Hydrolysis KW - Pretreatment Y1 - 2024 U6 - https://doi.org/10.3390/en17122938 SN - 1996-1073 N1 - Corresponding author: Nils Tippkötter VL - 17 IS - 12 PB - MDPI CY - Basel ER - TY - JOUR A1 - Golland, Alexander T1 - Bußgelder bei Datenschutzverstößen durch Unternehmen JF - NJW Neue Juristische Wochenschrift N2 - Bald eine Dekade ist es her, dass diese annähernd mantraartig wiederholte Phrase Unternehmen zur Umsetzung datenschutzrechtlicher Vorgaben incentivierte. Was ist davon geblieben? Nur wenige in Deutschland verhängte Bußgelder erreichten Millionenhöhe. Hintergrund ist (auch) das deutsche Ordnungswidrigkeitenrecht, welches in einem Spannungsverhältnis zu den Vorgaben der DS-GVO steht. Ein Bußgeldbescheid der Berliner Datenschutzaufsicht gegen die Deutsche Wohnen sollte Auslöser eines langen, fortdauernden Rechtsstreits werden. Auf Vorlage des KG hatte der EuGH in der Rechtssache C-807/21 („Deutsche Wohnen“) erstmals Gelegenheit, sich zur Frage der Bußgeldhaftung zu positionieren. Y1 - 2024 SN - 0341-1915 IS - 6 SP - 325 EP - 329 PB - Beck CY - München ER - TY - JOUR A1 - Golland, Alexander A1 - Schröer, Jan-Erik T1 - Error 404 im Vergabeverfahren: Datenschutz nicht gefunden? JF - VergabeR N2 - Das Thema Datenschutz wurde bei der öffentlichen Auftragsvergabe bislang vor allem in Bezug auf Drittlandtransfers personenbezogener Daten in die USA diskutiert. Jedoch spielt der Datenschutz für das Vergabeverfahren und für die Ausführung datenschutzrelevanter Leistungen generell eine wesentliche Rolle. Gleichwohl herrschen bislang unter öffentlichen Auftraggebern Schwierigkeiten, datenschutzrechtlich relevante Fallkonstellationen zu erkennen, die möglichen Risiken daraus abzuleiten und, sofern dies gelingt, diesen Risiken angemessen zu begegnen. Der vorliegende Beitrag befasst sich mit der datenschutzrechtlichen Verantwortlichkeit, ihren Folgen und den daraus resultierenden Konsequenzen für die Gestaltung von Vergabeverfahren und Vergabeunterlagen. Y1 - 2024 SN - 2366-2247 IS - 2 SP - 75 EP - 92 PB - Wolters Kluwer CY - Köln ER - TY - JOUR A1 - Adels, Klaudia A1 - Monakhova, Yulia T1 - Low-field NMR spectroscopic study of e-cigarettes: Is determination of only nicotine and organic carrier solvents possible? JF - Microchemical Journal N2 - Electronic cigarettes (e-cigarettes) have become popular worldwide with the market growing exponentially in some countries. The absence of product standards and safety regulations requires urgent development of analytical methodologies for the holistic control of the growing diversity of such products. An approach based on low-field nuclear magnetic resonance (LF-NMR) at 80 MHz is presented for the simultaneous determination of key parameters: carrier solvents (vegetable glycerine (VG), propylene glycol (PG) and water), total nicotine as well as free-base nicotine fraction. Moreover, qualitative and quantitative determination of fourteen weak organic acids deliberately added to enhance sensory characteristics of e-cigarettes was possible. In most cases these parameters can be rapidly and conveniently determined without using any sample manipulation such as dilution, extraction or derivatization steps. The method was applied for 37 authentic e-cigarettes samples. In particular, eight different organic acids with the content up to 56 mg/mL were detected. Due to its simplicity, the method can be used in routine regulatory control as well as to study release behaviour of nicotine and other e-cigarettes constituents in different products. KW - Electronic cigarettes KW - Low field NMR KW - Carrier solvents KW - Free-base nicotine KW - Weak organic acids Y1 - 2024 U6 - https://doi.org/10.1016/j.microc.2024.110859 SN - 1095-9149 N1 - Corresponding author: Yulia Monakhova VL - 203 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Schopen, Oliver A1 - Shah, Neel A1 - Esch, Thomas A1 - Shabani, Bahman T1 - Critical quantitative evaluation of integrated health management methods for fuel cell applications JF - International Journal of Hydrogen Energy N2 - Online fault diagnostics is a crucial consideration for fuel cell systems, particularly in mobile applications, to limit downtime and degradation, and to increase lifetime. Guided by a critical literature review, in this paper an overview of Health management systems classified in a scheme is presented, introducing commonly utilised methods to diagnose FCs in various applications. In this novel scheme, various Health management system methods are summarised and structured to provide an overview of existing systems including their associated tools. These systems are classified into four categories mainly focused on model-based and non-model-based systems. The individual methods are critically discussed when used individually or combined aimed at further understanding their functionality and suitability in different applications. Additionally, a tool is introduced to evaluate methods from each category based on the scheme presented. This tool applies the technique of matrix evaluation utilising several key parameters to identify the most appropriate methods for a given application. Based on this evaluation, the most suitable methods for each specific application are combined to build an integrated Health management system. KW - Fuel cell KW - Health management system KW - Online diagnostic KW - Fault detection KW - Non-model-based Evaluation Y1 - 2024 U6 - https://doi.org/10.1016/j.ijhydene.2024.05.156 SN - 0360-3199 VL - 70 SP - 370 EP - 388 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Timme, Michael T1 - Kaufvertrag: Das Zusammentreffen von Beschaffenheitsvereinbarung und Gewährleistungsausschluss – ein Spannungsverhältnis JF - Monatsschrift für Deutsches Recht N2 - Wer A sagt, muss zumindest im Kaufrecht nicht immer B sagen: Es kommt nicht selten vor, dass sich in einem Kaufvertrag einerseits ein wirksamer Ausschluss der Gewährleistung des Verkäufers für Sachmängel findet, die Parteien aber andererseits gleichwohl eine Beschaffenheitsvereinbarung für bestimmte Eigenschaften vertraglich festlegen. In diesem Problemfeld führt eine aktuelle Entscheidung des BGH zu weiteren Klärungen für die Praxis (BGH, Urt. v. 10.4.2024 – VIII ZR 161/23, MDR 2024, 706). Der folgende Beitrag setzt sich mit den vielfältigen Aspekten der Entscheidung auseinander und erläutert, aus welchen Gründen der BGH dem Käufer einige goldene Brücken für einen Schadensersatzanspruch gebaut hat. Y1 - 2024 U6 - https://doi.org/10.9785/mdtr-2024-781203 SN - 2194-4202 (online) SN - 0340-1812 (print) VL - 78 IS - 12 SP - 745 EP - 748 PB - Verlag Dr. Otto Schmidt CY - Köln ER - TY - JOUR A1 - Yoshinobu, Tatsuo A1 - Miyamoto, Ko-ichiro A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - Field-effect sensors combined with the scanned light pulse technique: from artificial olfactory images to chemical imaging technologies JF - Chemosensors N2 - The artificial olfactory image was proposed by Lundström et al. in 1991 as a new strategy for an electronic nose system which generated a two-dimensional mapping to be interpreted as a fingerprint of the detected gas species. The potential distribution generated by the catalytic metals integrated into a semiconductor field-effect structure was read as a photocurrent signal generated by scanning light pulses. The impact of the proposed technology spread beyond gas sensing, inspiring the development of various imaging modalities based on the light addressing of field-effect structures to obtain spatial maps of pH distribution, ions, molecules, and impedance, and these modalities have been applied in both biological and non-biological systems. These light-addressing technologies have been further developed to realize the position control of a faradaic current on the electrode surface for localized electrochemical reactions and amperometric measurements, as well as the actuation of liquids in microfluidic devices. KW - visualization KW - light-addressing technologies KW - scanned light pulse technique KW - field-effect structure KW - MOS KW - metal-oxide-semiconductor structure KW - catalytic metal KW - electronic nose KW - gas sensor KW - artificial olfactory image Y1 - 2024 U6 - https://doi.org/10.3390/chemosensors12020020 SN - 2227-9040 N1 - This article belongs to the Special Issue "An Exciting Journey of Chemical Sensors and Biosensors: A Theme Issue in Honor of Professor Ingemar Lundström" Corresponding author: Tatsuo Yoshinobu, Michael J. Schöning VL - 12 IS - 2 PB - MDPI CY - Basel ER -