TY  - JOUR
A1  - Aboulnaga, Elhussiny A.
A1  - Zou, Huibin
A1  - Selmer, Thorsten
A1  - Xian, Mo
T1  - Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16
JF  - Journal of Biotechnology
N2  - Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.
Y1  - 2018
U6  - https://doi.org/10.1016/j.jbiotec.2018.03.007
SN  - 0168-1656
VL  - 274
SP  - 15
EP  - 27
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Richter, Charlotte
A1  - Braunstein, Bjoern
A1  - Stäudle, Benjamin
A1  - Attias, Julia
A1  - Suess, Alexander
A1  - Weber, T.
A1  - Rittweger, Joern
A1  - Green, David A.
A1  - Albracht, Kirsten
T1  - In vivo fascicle length of the gastrocnemius muscle during walking in simulated martian gravity using two different body weight support devices
T2  - 23rd Annual Congress of the European College of Sport Science, Dublin, Irland
Y1  - 2018
ER  - 
TY  - CHAP
A1  - Finger, Felix
A1  - Braun, Carsten
A1  - Bil, Cees
T1  - An Initial Sizing Methodology for Hybrid-Electric Light Aircraft
T2  - AIAA AVIATION Forum 2018 Aviation Technology, Integration, and Operations Conference, Atlanta, Georgia, June 25 - 29, 2018
Y1  - 2018
U6  - https://doi.org/10.2514/6.2018-4229
ER  - 
TY  - CHAP
A1  - Fabo, Sabine
T1  - Eine dekonstruktive Erinnerungsarbeit
T2  - Doris Frohnapfel, bewaffnete Götter = Doris Frohnapfel, armed gods
Y1  - 2018
SN  - 978-3-9817241-2-7
N1  - Ausstellungskatalog Galerie M29: 09.11.2018 - 15.12.2018, Köln
SP  - 31
EP  - 35
PB  - RpB Richter-Brückner
CY  - Köln
ER  - 
TY  - RPRT
A1  - Fischedick, Manfred
A1  - Schoof, René
A1  - Hebel, Christoph
A1  - Merkens, Torsten
T1  - Sektorenkopplung als Herausforderung und Chance für das Energieland NRW : Handlungsoptionen und Ergebnispapier der Expertengruppe AG 4 „Sektoren- kopplung“ im Netzwerk Netze und Speicher der EnergieAgentur.NRW im Auftrag des Landes Nordrhein-Westfalen / EnergieAgentur.NRW GmbH
Y1  - 2018
ER  -