TY - JOUR A1 - Bertz, Morten A1 - Molinnus, Denise A1 - Schöning, Michael Josef A1 - Homma, Takayuki T1 - Real-time monitoring of H₂O₂ sterilization on individual bacillus atrophaeus spores by optical sensing with trapping Raman spectroscopy JF - Chemosensors N2 - Hydrogen peroxide (H₂O₂), a strong oxidizer, is a commonly used sterilization agent employed during aseptic food processing and medical applications. To assess the sterilization efficiency with H₂O₂, bacterial spores are common microbial systems due to their remarkable robustness against a wide variety of decontamination strategies. Despite their widespread use, there is, however, only little information about the detailed time-resolved mechanism underlying the oxidative spore death by H₂O₂. In this work, we investigate chemical and morphological changes of individual Bacillus atrophaeus spores undergoing oxidative damage using optical sensing with trapping Raman microscopy in real-time. The time-resolved experiments reveal that spore death involves two distinct phases: (i) an initial phase dominated by the fast release of dipicolinic acid (DPA), a major spore biomarker, which indicates the rupture of the spore’s core; and (ii) the oxidation of the remaining spore material resulting in the subsequent fragmentation of the spores’ coat. Simultaneous observation of the spore morphology by optical microscopy corroborates these mechanisms. The dependence of the onset of DPA release and the time constant of spore fragmentation on H₂O₂ shows that the formation of reactive oxygen species from H₂O₂ is the rate-limiting factor of oxidative spore death. KW - DPA (dipicolinic acid) KW - sterilization KW - Bacillus atrophaeus spores KW - optical trapping KW - Raman spectroscopy KW - optical sensor setup Y1 - 2023 U6 - http://dx.doi.org/10.3390/chemosensors11080445 SN - 2227-9040 N1 - This article belongs to the Special Issue "Biosensors and Chemical Sensors for Food and Healthcare Monitoring—Celebrating the 10th Anniversary" VL - 8 IS - 11 PB - MDPI CY - Basel ER - TY - JOUR A1 - Wendlandt, Tim A1 - Koch, Claudia A1 - Britz, Beate A1 - Liedek, Anke A1 - Schmidt, Nora A1 - Werner, Stefan A1 - Gleba, Yuri A1 - Vahidpour, Farnoosh A1 - Welden, Melanie A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System JF - Viruses N2 - Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes. KW - biosensor KW - horseradish peroxidase (HRP) KW - glucose oxidase (GOx) KW - enzyme cascade KW - turnip vein clearing virus (TVCV) KW - tobacco mosaic virus (TMV) Y1 - 2023 U6 - http://dx.doi.org/doi.org/10.3390/v15091951 SN - 1999-4915 N1 - This article belongs to the Special Issue "Tobamoviruses 2023" VL - 9 IS - 15 PB - MDPI CY - Basel ER - TY - GEN A1 - Steuer-Dankert, Linda A1 - Bernhard, Sebastian A1 - Langolf, Jessica A1 - Leicht-Scholten, Carmen T1 - About the paradox of sustainable production and what we can do about it! T2 - Joint SCORAI-ERSCP-WUR conference on transforming consumption-production systems toward just and sustainable futures (SCP23), July 5-8, 2023, Wageningen, The Netherlands N2 - Sustainability is playing an increasingly important role. Not least due to the definition of the sustainable development goals (SDGs) in the framework of the agenda 2030 by the United Nations (UN) in 2015 (United Nations, n.d.), it has become clear that the cooperation of different actors is needed to achieve the defined 17 goals. Industry, as a global actor, has a special role to play in this. In the course of sustainable production processes and chains, the industry is confronted with the responsibility of reflecting on the consequences of its own trade on an ecological, economic, and also social level and deriving measures that, according to the definition of sustainability (Hauff, 1987), will also enable future generations to satisfy their needs. While the ecological pillar of sustainability is already being addressed by different industrial initiatives (Deloitte, 2021), it is questionable to what extent the economic and, above all, the social pillars of sustainability also play a decisive role. Accordingly, it is questionable to what extent sustainability in its triad of social, ecological, and economic aspects is taken into account holistically at all, and thus to what extent the industry contributes to achieving the 17 goals defined by the UN. This paper presents a qualitative study that explores these questions. Interviewing 31 representatives from the manufacturing industry in Germany, results indicate a Paradox of Sustainable Production expressed by a theoretical reflection of the need for focusing on people in production processes on the one hand and a lack of addressing the social pillar of sustainability in concepts on the other hand. However, while it is a troublesome finding given the striking need for sustainable development (The-Sustainable-Development-Goals-Report-2022; Kropp 2019; von Hauff 2021; Roy and Singh 2017), the paradox directly lays out a path of resolving it. This is because, given its nature, we can see that we could resolve it via the implementation of strong educational efforts trying to help the respective people of the manufacturing industry to understand the holistic and interdependent character of sustainable development (The-Sustainable-Development-Goals-Report-2022). Y1 - 2023 N1 - Volltext auf dem Opus-Server verfügbar ER - TY - GEN A1 - Steuer-Dankert, Linda A1 - Berg-Postweiler, Julia A1 - Leicht-Scholten, Carmen T1 - One does not fit all: applying anti-bias trainings in academia T2 - Twenty-third international conference on diversity in organizations, communities & nations June 22 - 23, 2023 Toronto Metropolitan University, Rogers Communication Centre Toronto, Canada N2 - Anti-bias trainings are increasingly demanded and practiced in academia and industry to increase employees’ sensitivity to discrimination, racism, and diversity. Under the heading of “Diversity Management”, anti-bias trainings are mainly offered as one-off workshops intending to raise awareness of unconscious biases, create a diversity-affirming corporate culture, awake awareness of the potential of diversity, and ultimately enable the reflection of diversity in development processes. However, coming from childhood education, research and scientific articles on the sustainable effectiveness of anti-bias in adulthood, especially in academia, are very scarce. In order to fill this research gap, the paper explores how sustainable the effects of individual anti-bias trainings on the behavior of participants are. In order to investigate this, participant observation in a qualitative pre-post setting was conducted, analyzing anti-bias trainings in an academic context. Two observers actively participated in the training sessions and documented the activities and reflection processes of the participants. Overall, the results question the effectiveness of single anti-bias trainings and show that a target-group adaptive approach is mandatory due to the background of the approach in early childhood education. Therefore, it can be concluded that anti-bias work needs to be adapted to the target group’s needs and reality of life. Furthermore, the study reveals that single anti-bias trainings must be embedded in a holistic diversity management approach to stimulate sustainable reflection processes among the target group. This paper is one of the first to scientifically evaluate anti-bias training effectiveness, especially in engineering sciences and the university context. KW - Academia KW - Engineering Habitus KW - Organizational Culture KW - Diversity Management KW - Anti-Bias Y1 - 2023 ER - TY - CHAP A1 - Eggert, Mathias A1 - Zähl, Philipp M. A1 - Wolf, Martin R. A1 - Haase, Martin ED - Cooper, Kendra M.L. ED - Bucchiarone, Antonio T1 - Applying leaderboards for quality improvement in software development projects T2 - Software Engineering for Games in Serious Contexts N2 - Software development projects often fail because of insufficient code quality. It is now well documented that the task of testing software, for example, is perceived as uninteresting and rather boring, leading to poor software quality and major challenges to software development companies. One promising approach to increase the motivation for considering software quality is the use of gamification. Initial research works already investigated the effects of gamification on software developers and come to promising. Nevertheless, a lack of results from field experiments exists, which motivates the chapter at hand. By conducting a gamification experiment with five student software projects and by interviewing the project members, the chapter provides insights into the changing programming behavior of information systems students when confronted with a leaderboard. The results reveal a motivational effect as well as a reduction of code smells. KW - Leaderboard KW - Gamification KW - Software testing KW - Software development Y1 - 2023 SN - 978-3-031-33337-8 (Print) SN - 978-3-031-33338-5 (Online) U6 - http://dx.doi.org/10.1007/978-3-031-33338-5_11 SP - 243 EP - 263 PB - Springer CY - Cham ER - TY - THES A1 - Philippen, Jule T1 - bond(ed) : ein Projekt zum Kontaktabbruch in Familien N2 - Eines haben wir alle gemeinsam – Eine Familie. Die Bachelorarbeit „bond(ed)“ ist ein Projekt zum Kontaktabbruch in Familien. Familiäre Kontaktabbrüche sind heute keine Seltenheit mehr. Jede:r kennt jemanden, der davon betroffen ist, und doch wird nur selten darüber gesprochen. Schamgefühl, Angst und das Gefühl von Versagen spielen hierbei eine entscheidende Rolle. Doch wie kann es so weit kommen? Neun Personen haben sich bereit erklärt, ihre sehr persönlichen und emotionalen Geschichten zu teilen. Im Zuge dessen sind Fotos im jeweiligen Zuhause und in Interviewform niedergeschriebene Gespräche entstanden, welche unabhängig voneinander in zwei Büchern zu betrachten sind. Das Projekt „bond(ed)“ soll familiären Kontaktabbrüchen eine Plattform bieten und mit einem Tabuthema brechen. Es soll mit Vorurteilen aufräumen und ein Gefühl von Zusammengehörigkeit bei den Betroffenen wecken. KW - Familie KW - Kontaktabbruch KW - Fotografie KW - Interview KW - Menschen Y1 - 2023 PB - FH Aachen CY - Aachen ER - TY - THES A1 - Scheffel, Zeno Samuel T1 - Identität, Zusammenhalt, Zugehörigkeit : Die Exploration der Gestaltung von Fußballtrikots N2 - Das Fußballtrikot – Arbeitsbekleidung, Identifikationsmerkmal, Distinktionsmerkmal? Was ist das Fußballtrikot, was sind seine identitätsstiftenden Merkmale und welche Gestaltungsparameter prägen sein heutiges Aussehen? In dieser Publikation wird die Geschichte des Trikots ebenso wie die äußeren Einflüsse auf sein heutiges Erscheinungsbild dokumentiert und analysiert. Die Publikation definiert die verschiedenen Gestaltungsparameter, erläutert diese und ordnet sie ein. Ebenso wird ein Einblick in den kreativen Gestaltungsprozess von Fußballtrikots gewährt. Das Ziel dieser Publikation besteht darin, das Erscheinungsbild von Trikots zu verstehen und eine kritische Reflexion darüber zu ermöglichen, wie das Fußballtrikot individuell definiert wird. KW - Fußballtrikot KW - Trikot KW - Arbeitsbekleidung KW - Identifikation KW - Distinktion KW - Kommunikationsdesign Y1 - 2023 N1 - Für diese Arbeit steht kein Volltext zur Verfügung. PB - FH Aachen CY - Aachen ER - TY - THES A1 - Schlepütz, Nico T1 - News Flow : Nachrichten- und Informationsprozesse im digitalen Wandel N2 - "News Flow" Nachrichten- und Informationsprozesse im digitalen Wandel. In der digitalen Welt sind wir von Informationen aus unterschiedlichen Medien umgeben. Die ständige Verfügbarkeit führt zu Überforderung und Orientierungslosigkeit. Junge Menschen bevorzugen Streaming und Social Media gegenüber traditionellen Nachrichten. Angesichts globaler Krisen und Desinformation sind innovative Lösungen wichtig, um den Informationsfluss zu verbessern. "News Flow" ist eine digitale Anwendung für Alternativen des Nachrichtenkonsums. Sie berücksichtigt individuelle Bedürfnisse und nutzt moderne Technologien. Durch die visuelle Vermittlung wird das Interesse an Nachrichten gesteigert und junge Zielgruppen angesprochen. Diese Arbeit stellt sich den Herausforderungen des digitalen Wandels im Nachrichtenbereich und eröffnet eine neue Perspektive auf den Nachrichtenkonsum. KW - Informationsfluss KW - Good News Fokus KW - UX Design KW - UI Design KW - Alternative Konsumoption Y1 - 2023 N1 - Für diese Arbeit steht kein Volltext zur Verfügung. PB - FH Aachen CY - Aachen ER - TY - THES A1 - Schmidt, Lucie T1 - „Keine Schmerzen in der Regel“ : Aufklärungskampagne zur Früherkennung der Krankheit Endometriose N2 - Jede zehnte Frau hat Endometriose. Hierbei entstehen Zysten und Entzündungen, welche sich beispielsweise an den Eierstöcken ansiedeln können. Wie kann es also sein, dass so viele noch nie was von dieser Erkrankung gehört haben? Mit der online Aufklärungskampagne „ Keine Schmerzen in der Regel“ soll sich das ändern. In vielen Fällen werden Beschwerden der Endometriose fälschlicherweise als normale Menstruationsbeschwerden abgetan. Die Differenzierung zwischen normal und nicht normal misslingt. Die Aufmerksamkeit von Mädchen und Frauen, die sich in den nicht normalen Aspekten wiederfinden, soll erregt werden. Dafür werden vier Symptome visualisiert und gegenübergestellt. Zusätzlich bietet ein Schnellcheck weitere Informationen. Diese Kampagne soll dem Problem der Unbekanntheit von Endometriose entgegenwirken und ein gesellschaftliches Bewusstsein für sie schaffen. KW - Aufklärungskampagne KW - Endometriose KW - Krankheit KW - Kommunikationsdesign Y1 - 2023 N1 - Für diese Arbeit steht kein Volltext zur Verfügung. PB - FH Aachen CY - Aachen ER - TY - BOOK A1 - Heuermann, Holger T1 - Mikrowellentechnik : Feldsimulation, nichtlineare Schaltungstechnik, Komponenten und Subsysteme, Plasmatechnik, Antennen und Ausbreitung N2 - Das Lehrbuch behandelt alle Aspekte, die den aktuellen Stand der GHz-Technik betreffen. Das Buch behandelt die verschiedenen numerischen Feldsimulationsverfahren. Mit vielen modernen Themen. KW - Elektrotechnik KW - Hochfrequenztechnik KW - Feldsimulation KW - Plasmatechnik KW - Antennen Y1 - 2023 SN - 978-3-658-41286-9 U6 - http://dx.doi.org/10.1007/978-3-658-41287-6 PB - Springer Vieweg CY - Wiesbaden ET - 2. Auflage ER -