TY - JOUR A1 - Aboulnaga, E. A. A1 - Zou, H. A1 - Selmer, Thorsten A1 - Xian, M. T1 - Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16 JF - Journal of Biotechnology N2 - Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate. Y1 - 2018 U6 - https://doi.org/10.1016/j.jbiotec.2018.03.007 SN - 0168-1656 VL - 274 SP - 15 EP - 27 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Röth, Thilo A1 - Pielen, Michael A1 - Wolff, Klaus A1 - Lüdiger, Thomas T1 - Urbane Fahrzeugkonzepte für die Shared Mobility JF - Automobiltechnische Zeitschrift - ATZ N2 - Urbane Mobilitätskonzepte der Zukunft erfordern neue Unternehmensformen, idealerweise aus Old Economy und New Economy, sowie eine enge Anbindung an die gesellschaftsrelevante Zukunftsforschung. Für neue Fahrzeugkonzepte des Carsharing bedeutet dies, dass alle kostenverursachenden Faktoren erfasst und analysiert werden müssen. Die FH Aachen, share2drive und FEV geben einen Ausblick auf die zukünftige Fahrzeugklasse der Personal Public Vehicles als „Rolling Device“. Y1 - 2018 U6 - https://doi.org/10.1007/s35148-017-0176-8 SN - 0001-2785 VL - 120 IS - 1 SP - 18 EP - 23 PB - Springer Vieweg CY - Wiesbaden ER - TY - CHAP A1 - Scholl, Ingrid A1 - Suder, Sebastian A1 - Schiffer, Stefan T1 - Direct Volume Rendering in Virtual Reality T2 - Bildverarbeitung für die Medizin 2018 Y1 - 2018 SN - 978-3-662-56537-7 U6 - https://doi.org/10.1007/978-3-662-56537-7_79 SP - 297 EP - 302 PB - Springer Vieweg CY - Berlin ER - TY - JOUR A1 - Schirra, Julian A1 - Bissonnette, William A1 - Bramesfeld, Götz T1 - Wake-model effects on induced drag prediction of staggered boxwings JF - Aerospace Y1 - 2018 U6 - https://doi.org/10.3390/aerospace5010014 SN - 2226-4310 VL - 5 IS - 1 ER - TY - JOUR A1 - Druckenmüller, Katharina A1 - Günther, Klaus A1 - Elbers, Gereon T1 - Near-infrared spectroscopy (NIRS) as a tool to monitor exhaust air from poultry operations JF - Science of the Total Environment N2 - Intensive poultry operation systems emit a considerable volume of inorganic and organic matter in the surrounding environment. Monitoring cleaning properties of exhaust air cleaning systems and to detect small but significant changes in emission characteristics during a fattening cycle is important for both emission and fattening process control. In the present study, we evaluated the potential of near-infrared spectroscopy (NIRS) combined with chemometric techniques as a monitoring tool of exhaust air from poultry operation systems. To generate a high-quality data set for evaluation, the exhaust air of two poultry houses was sampled by applying state-of-the-art filter sampling protocols. The two stables were identical except for one crucial difference, the presence or absence of an exhaust air cleaning system. In total, twenty-one exhaust air samples were collected at the two sites to monitor spectral differences caused by the cleaning device, and to follow changes in exhaust air characteristics during a fattening period. The total dust load was analyzed by gravimetric determination and included as a response variable in multivariate data analysis. The filter samples were directly measured with NIR spectroscopy. Principal component analysis (PCA), linear discriminant analysis (LDA), and factor analysis (FA) were effective in classifying the NIR exhaust air spectra according to fattening day and origin. The results indicate that the dust load and the composition of exhaust air (inorganic or organic matter) substantially influence the NIR spectral patterns. In conclusion, NIR spectroscopy as a tool is a promising and very rapid way to detect differences between exhaust air samples based on still not clearly defined circumstances triggered during a fattening period and the availability of an exhaust air cleaning system. Y1 - 2018 U6 - https://doi.org/10.1016/j.scitotenv.2018.02.072 SN - 0048-9697 VL - 630 SP - 536 EP - 543 PB - Elsevier CY - Amsterdam ER - TY - BOOK A1 - Hüning, Felix T1 - Embedded Design For IoT With Renesas Synergy Y1 - 2018 N1 - gedruckt in der Bereichsbibliothek Eupener Str. vorhanden; Document No. R01PF0164ED0100 PB - Renesas Electronics CY - Düsseldorf ER - TY - JOUR A1 - Wollert, Jörg T1 - Echtzeit-Ethernet – Basis für Industrie 4.0 JF - Design & Elektronik Y1 - 2018 SN - 0933-8667 N1 - Echtzeit für Industrie 4.0. - Teil 2 PB - WEKA-Fachmedien CY - München ER - TY - JOUR A1 - Pilas, Johanna A1 - Yazici, Y. A1 - Selmer, Thorsten A1 - Keusgen, M. A1 - Schöning, Michael Josef T1 - Application of a portable multi-analyte biosensor for organic acid determination in silage JF - Sensors N2 - Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at −21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at −21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media. Y1 - 2018 U6 - https://doi.org/10.3390/s18051470 SN - 1424-8220 VL - 18 IS - 5 PB - MDPI CY - Basel ER - TY - JOUR A1 - Dantism, Shahriar A1 - Röhlen, Desiree A1 - Wagner, Torsten A1 - Wagner, Patrick A1 - Schöning, Michael Josef T1 - Optimization of Cell-Based Multi-Chamber LAPS Measurements Utilizing FPGA-Controlled Laser-Diode Modules JF - physica status solidi a : applications and materials sciences N2 - A light-addressable potentiometric sensor (LAPS) is a field-effect-based potentiometric device, which detects concentration changes of an analyte solution on the sensor surface in a spatially resolved way. It uses a light source to generate electron–hole pairs inside the semiconductor, which are separated in the depletion region due to an applied bias voltage across the sensor structure and hence, a surface-potential-dependent photocurrent can be read out. However, depending on the beam angle of the light source, scattering effects can occur, which influence the recorded signal in LAPS-based differential measurements. To solve this problem, a novel illumination unit based on a field programmable gate array (FPGA) consisting of 16 small-sized tunable infrared laser-diode modules (LDMs) is developed. Due to the improved focus of the LDMs with a beam angle of only 2 mrad, undesirable scattering effects are minimized. Escherichia coli (E. coli) K12 bacteria are used as a test microorganism to study the extracellular acidification on the sensor surface. Furthermore, a salt bridge chamber is built up and integrated with the LAPS system enabling multi-chamber differential measurements with a single Ag/AgCl reference electrode. Y1 - 2018 U6 - https://doi.org/10.1002/pssa.201800058 SN - 1862-6319 VL - 215 IS - 15 SP - Article number 1800058 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Schreiber, Marc A1 - Kraft, Bodo A1 - Zündorf, Albert T1 - NLP Lean Programming Framework: Developing NLP Applications More Effectively T2 - Proceedings of NAACL-HLT 2018: Demonstrations, New Orleans, Louisiana, June 2 - 4, 2018 N2 - This paper presents NLP Lean Programming framework (NLPf), a new framework for creating custom natural language processing (NLP) models and pipelines by utilizing common software development build systems. This approach allows developers to train and integrate domain-specific NLP pipelines into their applications seamlessly. Additionally, NLPf provides an annotation tool which improves the annotation process significantly by providing a well-designed GUI and sophisticated way of using input devices. Due to NLPf’s properties developers and domain experts are able to build domain-specific NLP applications more efficiently. NLPf is Opensource software and available at https:// gitlab.com/schrieveslaach/NLPf. Y1 - 2018 U6 - https://doi.org/10.18653/v1/N18-5001  ER -