TY - BOOK A1 - Lauth, Jakob A1 - Kowalczyk, Jürgen T1 - Thermodynamik : eine Einführung Y1 - 2015 SN - 978-3-662-46228-7 U6 - https://doi.org/10.1007/978-3-662-46229-4 PB - Springer Spektrum CY - Berlin ER - TY - JOUR A1 - Eckert, Alexander A1 - Abbasi, Mozhdeh A1 - Mang, Thomas A1 - Saalwächter, Kay A1 - Walther, Andreas T1 - Structure, Mechanical Properties, and Dynamics of Polyethylenoxide/Nanoclay Nacre-Mimetic Nanocomposites JF - Macromolecules N2 - Nacre-mimetic nanocomposites based on high fractions of synthetic high-aspect-ratio nanoclays in combination with polymers are continuously pushing boundaries for advanced material properties, such as high barrier against oxygen, extraordinary mechanical behavior, fire shielding, and glass-like transparency. Additionally, they provide interesting model systems to study polymers under nanoconfinement due to the well-defined layered nanocomposite arrangement. Although the general behavior in terms of forming such layered nanocomposite materials using evaporative self-assembly and controlling the nanoclay gallery spacing by the nanoclay/polymer ratio is understood, some combinations of polymer matrices and nanoclay reinforcement do not comply with the established models. Here, we demonstrate a thorough characterization and analysis of such an unusual polymer/nanoclay pair that falls outside of the general behavior. Poly(ethylene oxide) (PEO) and sodium fluorohectorite form nacre-mimetic, lamellar nanocomposites that are completely transparent and show high mechanical stiffness and high gas barrier, but there is only limited expansion of the nanoclay gallery spacing when adding increasing amounts of polymer. This behavior is maintained for molecular weights of PEO varied over four orders of magnitude and can be traced back to depletion forces. By careful investigation via X-ray diffraction and proton low-resolution solid-state NMR, we are able to quantify the amount of mobile and immobilized polymer species in between the nanoclay galleries and around proposed tactoid stacks embedded in a PEO matrix. We further elucidate the unusual confined polymer dynamics, indicating a relevant role of specific surface interactions. Y1 - 2020 U6 - https://doi.org/10.1021/acs.macromol.9b01931 SN - 1520-5835 VL - 53 IS - 5 SP - 1716 EP - 1725 PB - ACS Publications CY - Washington, DC ER - TY - JOUR A1 - Lowis, Carsten A1 - Ferguson, Simon A1 - Paulßen, Elisabeth A1 - Hoehr, Cornelia T1 - Improved Sc-44 production in a siphon-style liquid target on a medical cyclotron JF - Applied Radiation and Isotopes Y1 - 2021 U6 - https://doi.org/10.1016/j.apradiso.2021.109675 SN - 0969-8043 VL - 172 IS - Art. 109675 PB - Elsevier CY - Amsterdam ER - TY - BOOK A1 - Lauth, Jakob T1 - Physikalische Chemie kompakt KW - Physikalische Chemie Y1 - 2022 SN - 978-3-662-64587-1 U6 - https://doi.org/https://doi.org/10.1007/978-3-662-64588-8 PB - Springer Spektrum CY - Berlin ER - TY - JOUR A1 - Haeger, Gerrit A1 - Grankin, Alina A1 - Wagner, Michaela T1 - Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology JF - Applied Research N2 - Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems. KW - aspergillus KW - CRISPR/Cas9 KW - filamentous fungi KW - genome engineering Y1 - 2023 U6 - https://doi.org/10.1002/appl.202200106 SN - 2702-4288 IS - Early View SP - 1 EP - 15 PB - Wiley-VCH ER - TY - BOOK A1 - Lauth, Jakob T1 - Physical chemistry in a nutshell: Basics for engineers and scientists N2 - This book is based on a multimedia course for biological and chemical engineers, which is designed to trigger students' curiosity and initiative. A solid basic knowledge of thermodynamics and kinetics is necessary for understanding many technical, chemical, and biological processes. The one-semester basic lecture course was divided into 12 workshops (chapters). Each chapter covers a practically relevant area of physical chemistry and contains the following didactic elements that make this book particularly exciting and understandable: - Links to Videos at the start of each chapter as preparation for the workshop - Key terms (in bold) for further research of your own - Comprehension questions and calculation exercises with solutions as learning checks - Key illustrations as simple, easy-to-replicate blackboard pictures Humorous cartoons for each workshop (by Faelis) additionally lighten up the text and facilitate the learning process as a mnemonic. To round out the book, the appendix includes a summary of the most popular experiments in basic physical chemistry courses, as well as suggestions for designing workshops with exhibits, experiments, and "questions of the day." Suitable for students minoring in chemistry; chemistry majors are sure to find this slimmed-down, didactically valuable book helpful as well. The book is excellent for self-study. KW - Physical chemistry KW - Thermodynamics as minor KW - Physical chemistry starters KW - Minor chemistry KW - Physical chemistry basics Y1 - 2023 SN - 978-3-662-67636-3 (Softcover) SN - 978-3-662-67637-0 (eBook) U6 - https://doi.org/10.1007/978-3-662-67637-0 PB - Springer CY - Berlin ER - TY - JOUR A1 - Dellmann, Sophia Florence A1 - Glorius, J. A1 - Litvinov, Yu A. A1 - Reifarth, R. A1 - Al-Khasawneh, Kafa A1 - Aliotta, M. A1 - Bott, L. A1 - Brückner, Benjamin A1 - Bruno, C. G. A1 - Chen, Ruijiu A1 - Davinson, T. A1 - Dickel, T. A1 - Dillmann, Iris A1 - Dmytriev, D. A1 - Erbacher, P. A1 - Freire-Fernández, D. A1 - Forstner, Oliver A1 - Geissel, H. A1 - Göbel, K. A1 - Griffin, Christopher J. A1 - Grisenti, R. A1 - Gumberidze, Alexandre A1 - Haettner, Emma A1 - Hagmann, Siegbert A1 - Heil, M. A1 - Heß, R. A1 - Hillenbrand, P.-M. A1 - Joseph, R. A1 - Jurado, B. A1 - Kozhuharov, Christophor A1 - Kulikov, I. A1 - Löher, Bastian A1 - Langer, Christoph A1 - Leckenby, Guy A1 - Lederer-Woods, C. A1 - Lestinsky, M. A1 - Litvinov, S. A. A1 - Lorenz, B. A. A1 - Lorenz, E. A1 - Marsh, J. A1 - Menz, Esther Babette A1 - Morgenroth, T. A1 - Petridis, N. A1 - Pibernat, Jerome A1 - Popp, U. A1 - Psaltis, Athanasios A1 - Sanjari, Shahab A1 - Scheidenberger, C. A1 - Sguazzin, M. A1 - Sidhu, Ragandeep Singh A1 - Spillmann, Uwe A1 - Steck, M. A1 - Stöhlker, T. A1 - Surzhykov, A. A1 - Swartz, J. A. A1 - Törnqvist, H. A1 - Varga, L. A1 - Vescovi, Diego A1 - Weick, H. A1 - Weigand, M. A1 - Woods, P. A1 - Xing, Y. A1 - Yamaguchi, Taiyo T1 - Proton capture on stored radioactive ¹¹⁸Te ions JF - EPJ Web of Conferences N2 - Experimental determination of the cross sections of proton capture on radioactive nuclei is extremely difficult. Therefore, it is of substantial interest for the understanding of the production of the p-nuclei. For the first time, a direct measurement of proton-capture cross sections on stored, radioactive ions became possible in an energy range of interest for nuclear astrophysics. The experiment was performed at the Experimental Storage Ring (ESR) at GSI by making use of a sensitive method to measure (p,γ) and (p,n) reactions in inverse kinematics. These reaction channels are of high relevance for the nucleosyn-thesis processes in supernovae, which are among the most violent explosions in the universe and are not yet well understood. The cross section of the ¹¹⁸Te(p,γ) reaction has been measured at energies of 6 MeV/u and 7 MeV/u. The heavy ions interacted with a hydrogen gas jet target. The radiative recombination process of the fully stripped ¹¹⁸Te ions and electrons from the hydrogen target was used as a luminosity monitor. An overview of the experimental method and preliminary results from the ongoing analysis will be presented. Y1 - 2023 U6 - https://doi.org/10.1051/epjconf/202327911018 SN - 2100-014X N1 - Volume 279, 2023. Nuclear Physics in Astrophysics – X (NPA-X 2022). VL - 279 IS - Article Number: 11018 SP - 1 EP - 5 PB - EDP Sciences ER - TY - INPR A1 - Greiner, Lasse A1 - Jeromin, Günter Erich A1 - Sithole, Patience A1 - Petersen, Soenke T1 - Preprint: Studies on the enzymatic reduction of levulinic acid using Chiralidon-R and Chiralidon-S T2 - ChemRxiv N2 - The enzymatic reduction of levulinic acid by the chiral catalysts Chiralidon-R and Chiralidon-S which are commercially available superabsorbed alcohol dehydrogenases is described. The Chiralidon®-R/S reduces the levulinic acid to the (R,S)-4-hydroxy valeric acid and the (R)- or (S)- gamma-valerolactone. KW - Levulinic acid KW - Chiralidon-R KW - Chiralidon-S KW - 4-hydroxy valeric acid KW - (R)- or (S)- gamma-valerolactone Y1 - 2023 U6 - https://doi.org/10.26434/chemrxiv-2023-jlvcv ER - TY - JOUR A1 - Degering, Christian A1 - Eggert, Thorsten A1 - Puls, Michael A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Jaeger, Karl-Erich T1 - Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides JF - Applied and environmental microbiology N2 - Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains. Y1 - 2010 U6 - https://doi.org/10.1128/AEM.01146-10 SN - 1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print) VL - 76 IS - 19 SP - 6370 EP - 6378 PB - American Society for Microbiology CY - Washington, DC ER - TY - JOUR A1 - Deppe, Veronika Maria A1 - Bongaerts, Johannes A1 - O'Connell, Timothy A1 - Maurer, Karl-Heinz A1 - Meinhardt, Friedhelm T1 - Enzymatic deglycation of Amadori products in bacteria JF - Applied microbiology and biotechnology Y1 - 2011 SN - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print) VL - Vol. 90 IS - Iss. 2 SP - 399 EP - 406 PB - Springer CY - Berlin ER -