TY - JOUR A1 - Muschallik, Lukas A1 - Kipp, Carina Ronja A1 - Recker, Inga A1 - Bongaerts, Johannes A1 - Pohl, Martina A1 - Gelissen, Melanie A1 - Schöning, Michael Josef A1 - Selmer, Thorsten A1 - Siegert, Petra T1 - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase JF - Journal of Biotechnology N2 - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled. Y1 - 2020 SN - 2590-1559 U6 - https://doi.org/10.1016/j.jbiotec.2020.09.016 VL - 202 IS - Vol. 324 SP - 61 EP - 70 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Hunker, Jan L. A1 - Gossmann, Matthias A1 - Raman, Aravind Hariharan A1 - Linder, Peter T1 - Artificial neural networks in cardiac safety assessment: Classification of chemotherapeutic compound effects on hiPSC-derived cardiomyocyte contractility JF - Journal of Pharmacological and Toxicological Methods Y1 - 2021 U6 - https://doi.org/10.1016/j.vascn.2021.107044 SN - 1056-8719 VL - 111 IS - Article number 107044 PB - Elsevier CY - New York ER - TY - BOOK A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül A1 - Zhubanova, Azhar A. A1 - Digel, Ilya ED - Artmann, Gerhard ED - Temiz Artmann, Aysegül ED - Zhubanova, Azhar A. ED - Digel, Ilya T1 - Biological, physical and technical basics of cell engineering Y1 - 2018 SN - 978-981-10-7903-0 PB - Springer CY - Singapore ER - TY - JOUR A1 - Staat, Manfred T1 - Local and global collapse pressure of longitudinally flawed pipes and cylindrical vessels N2 - Limit loads can be calculated with the finite element method (FEM) for any component, defect geometry, and loading. FEM suggests that published long crack limit formulae for axial defects under-estimate the burst pressure for internal surface defects in thick pipes while limit loads are not conservative for deep cracks and for pressure loaded crack-faces. Very deep cracks have a residual strength, which is modelled by a global collapse load. These observations are combined to derive new analytical local and global collapse loads. The global collapse loads are close to FEM limit analyses for all crack dimensions. KW - Finite-Elemente-Methode KW - Grenzwertberechnung KW - Axialbelastung KW - FEM KW - Grenzwertberechnung KW - Axialbelastung KW - Traglastanalyse KW - Limit analysis KW - Global and local collapse KW - Axially cracked pipe KW - Pressure loaded crack-face Y1 - 2005 ER - TY - CHAP A1 - Tran, Thanh Ngoc A1 - Staat, Manfred A1 - Kreißig, R. T1 - Finite element shakedown and limit reliability analysis of thin shells N2 - A procedure for the evaluation of the failure probability of elastic-plastic thin shell structures is presented. The procedure involves a deterministic limit and shakedown analysis for each probabilistic iteration which is based on the kinematical approach and the use the exact Ilyushin yield surface. Based on a direct definition of the limit state function, the non-linear problems may be efficiently solved by using the First and Second Order Reliabiblity Methods (Form/SORM). This direct approach reduces considerably the necessary knowledge of uncertain technological input data, computing costs and the numerical error. In: Computational plasticity / ed. by Eugenio Onate. Dordrecht: Springer 2007. VII, 265 S. (Computational Methods in Applied Sciences ; 7) (COMPLAS IX. Part 1 . International Center for Numerical Methods in Engineering (CIMNE)). ISBN 978-1-402-06576-7 S. 186-189 KW - Finite-Elemente-Methode KW - Limit analysis KW - shakedown analysis KW - Exact Ilyushin yield surface KW - Random variable KW - First Order Reliabiblity Method Y1 - 2007 ER - TY - JOUR A1 - Staat, Manfred T1 - Plastic collapse analysis of longitudinally flawed pipes and vessels N2 - Improved collapse loads of thick-walled, crack containing pipes and vessels are suggested. Very deep cracks have a residual strength which is better modelled by a global limit load. In all burst tests, the ductility of pressure vessel steels was sufficiently high whereby the burst pressure could be predicted by limit analysis with no need to apply fracture mechanics. The relative prognosis error increases however, for long and deep defects due to uncertainties of geometry and strength data. KW - Druckbehälter KW - Stahl KW - Druckbelastung KW - Druckbeanspruchung KW - Rohr KW - Rohrbruch KW - Druckbehälter KW - Stahl KW - Druckbelastung KW - Druckbeanspruchung KW - Rohrbruch KW - Fehlerstellen KW - pipes KW - vessels KW - load limit KW - burst tests KW - burst pressure KW - flaw Y1 - 2004 ER - TY - CHAP A1 - Duong, Minh Tuan A1 - Staat, Manfred ED - Onate, E. T1 - A face-based smoothed finite element method for hyperelastic models and tissue growth T2 - 11th World Congress on Computational Mechanics (WCCM XI) ; 5th European Conference on Computational Mechanics (ECCM V) ; 6th European Conference on Computational Fluid Dynamics (ECFD VI) ; July 20-25, 2014, Barcelona Y1 - 2014 SP - 1 EP - 12 ER - TY - CHAP A1 - Staat, Manfred T1 - Design by Analysis of Pressure Components by non-linear Optimization N2 - This paper presents the direct route to Design by Analysis (DBA) of the new European pressure vessel standard in the language of limit and shakedown analysis (LISA). This approach leads to an optimization problem. Its solution with Finite Element Analysis is demonstrated for some examples from the DBA-Manual. One observation from the examples is, that the optimisation approach gives reliable and close lower bound solutions leading to simple and optimised design decision. KW - Analytischer Zulaessigkeitsnachweis KW - FEM KW - Einspiel-Analyse KW - design-by-analysis KW - finite element analysis KW - limit and shakedown analysis Y1 - 2003 ER - TY - JOUR A1 - Staat, Manfred A1 - Schwartz, M. A1 - Lang, H. A1 - Wirtz, K. A1 - Heitzer, M. T1 - Design by Analysis of Pressure Components by non-linear Optimization JF - The 10th International Conference on Pressure Vessel Technology, July 7-10, 2003, Vienna, Austria, Proceedings ICPVT-10 / Zeman, J. L. [ed] Y1 - 2003 SN - 3950152814 SP - 59 EP - 65 PB - ÖGS, Österreichische Gesellschaft für Schweißtechnik CY - Wien ER - TY - JOUR A1 - Staat, Manfred A1 - Heitzer, M. T1 - Limit and Shakedown Analysis with Uncertain Data JF - Stochastic optimization techniques : numerical methods and technical applications / Marti, K. [ed] Y1 - 2002 SN - 3-540-42889-5 SP - 241 EP - 254 PB - Springer CY - Heidelberg ER -