TY  - CHAP
A1  - Barek, Jiri
A1  - Fischer, Jan
A1  - Navratil, Tomas
A1  - Peckova, Karolina
A1  - Yosypchuk, Bogdan
T1  - Silver solid amalgam electrodes as sensors for chemical carcinogens
N2  - The applicability of differential pulse voltammetry (DPV) and adsorptive stripping voltammetry (AdSV) at a non-toxic meniscus-modified silver solid amalgam electrode (m-AgSAE) for the determination of trace amounts of genotoxic substances was demonstrated on the determination of micromolar and submicromolar concentrations of 3-nitrofluoranthene using methanol - 0.01 mol L-1 NaOH (9:1) mixture as a base electrolyte and of Ostazine Orange using 0.01 mol L-1 NaOH as a base electrolyte.
KW  - Biosensor
KW  - Solid amalgam electrodes
KW  - voltammetry
KW  - carcinogens
KW  - 3-nitrofluoranthene
KW  - Ostazine Orange
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1554
ER  - 
TY  - CHAP
A1  - Baronas, Romas
A1  - Ivanauskas, Feliksas
A1  - Kulys, Juozas
T1  - Mathematical modeling of biosensors based on an array of enzyme microreactors
N2  - This paper presents a two-dimensional-in-space mathematical model of biosensors based on an array of enzyme microreactors immobilised on a single electrode. The modeling system acts under amperometric conditions. The microreactors were modeled by particles and by strips. The model is based on the diffusion equations containing a nonlinear term related to the Michaelis-Menten kinetics of the enzymatic reaction. The model involves three regions: an array of enzyme microreactors where enzyme reaction as well as mass transport by diffusion takes place, a diffusion limiting region where only the diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Using computer simulation, the influence of the geometry of the microreactors and of the diffusion region on the biosensor response was investigated. The digital simulation was carried out using the finite difference technique.
KW  - Biosensor
KW  - Reaction-diffusion
KW  - modeling biosensor
KW  - microreactor
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1569
ER  - 
TY  - CHAP
A1  - Lei, Yu
A1  - Mulchandani, Priti
A1  - Chen, Wilfred
A1  - Mulchandani, Ashok
T1  - Biosensor for direct determination of fenitrothion and EPN using recombinant Pseudomonas putida JS444 with surface expressed organophosphorus hydrolase. 1. modified clark oxygen electrode
N2  - This paper reports a first microbial biosensor for rapid and cost-effective determination of organophosphorus pesticides fenitrothion and EPN. The biosensor consisted of recombinant PNP-degrading/oxidizing bacteria Pseudomonas putida JS444 anchoring and displaying organophosphorus hydrolase (OPH) on its cell surface as biological sensing element and a dissolved oxygen electrode as the transducer. Surfaceexpressed OPH catalyzed the hydrolysis of fenitrothion and EPN to release 3-methyl-4-nitrophenol and p-nitrophenol, respectively, which were oxidized by the enzymatic machinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen, which was measured and correlated to the concentration of organophosphates. Under the optimum operating conditions, the biosensor was able to measure as low as 277 ppb of fenitrothion and 1.6 ppm of EPN without interference from phenolic compounds and other commonly used pesticides such as carbamate pesticides, triazine herbicides and organophosphate pesticides without nitrophenyl substituent. The applicability of the biosensor to lake water was also demonstrated.
KW  - Biosensor
KW  - Organophosphorus
KW  - fenitrothion
KW  - EPN
KW  - biosensor
KW  - Pseudomonas putida
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:hbz:a96-opus-1573
ER  -