TY - CHAP A1 - Bung, Daniel Bernhard T1 - A comparative study of self-aerated stepped spillway and smooth invert chute flow: the effect of step-induced macro roughness T2 - 5th Chinese-German Joint Symposium on Hydraulic and Ocean Engineering : CG JOINT 2010 Y1 - 2010 SN - 978-7-5618-3671-2 SP - 451 EP - 456 PB - Univ. Press CY - Tianjin ER - TY - JOUR A1 - Ribitsch, D. A1 - Karl, W. A1 - Birner-Gruenberger, R. A1 - Gruber, K. A1 - Eiteljoerg, I. A1 - Remler, P. A1 - Wieland, S. A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schwab, H. T1 - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli JF - Journal of biotechnology N2 - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease. Y1 - 2010 U6 - https://doi.org/10.1016/j.jbiotec.2010.09.947 SN - 1873-4863 (E-Journal); 0168-1656 (Print) VL - 150 IS - 3 SP - 408 EP - 416 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Buda, Aurel A1 - Schürmann, Volker A1 - Wollert, Jörg T1 - Wireless technologies in factory automation T2 - Factory automation / ed. by Javier Silvestre-Blanes Y1 - 2010 SN - 978-953-7619-42-8 SP - 29 EP - 50 PB - Intech CY - London ER - TY - CHAP A1 - Schürmann, Volker A1 - Buda, Aurel A1 - Jonker, Stefan A1 - Palmhof, Norman A1 - Wollert, Jörg T1 - Wireless in future automotive applications T2 - Mobile and wireless communications network layer and circuit level design / ed. by Salma Ait Fares ... Y1 - 2010 SN - 978-953-307-042-1 SP - 71 EP - 92 PB - Intech CY - London ER - TY - CHAP A1 - Schürmann, Volker A1 - Leidinger, R. A1 - Wollert, Jörg T1 - Simplified rapid-prototyping with automation technology T2 - Automobiles and sustainable mobility : FISITA 2010 World Automotive Congress, 30 May - 4 June, Budapest, Hungary ; proceedings Y1 - 2010 SN - 978-963-9058-29-3 SP - F2010C093 ER - TY - JOUR A1 - Vogt, C. A1 - Mottaghy, Darius A1 - Wolf, A. A1 - Rath, V. A1 - Pechnig, R. A1 - Clauser, C. T1 - Reducing temperature uncertainties by stochastic geothermal reservoir modelling JF - Geophysical Journal International N2 - Quantifying and minimizing uncertainty is vital for simulating technically and economically successful geothermal reservoirs. To this end, we apply a stochastic modelling sequence, a Monte Carlo study, based on (i) creating an ensemble of possible realizations of a reservoir model, (ii) forward simulation of fluid flow and heat transport, and (iii) constraining post-processing using observed state variables. To generate the ensemble, we use the stochastic algorithm of Sequential Gaussian Simulation and test its potential fitting rock properties, such as thermal conductivity and permeability, of a synthetic reference model and—performing a corresponding forward simulation—state variables such as temperature. The ensemble yields probability distributions of rock properties and state variables at any location inside the reservoir. In addition, we perform a constraining post-processing in order to minimize the uncertainty of the obtained distributions by conditioning the ensemble to observed state variables, in this case temperature. This constraining post-processing works particularly well on systems dominated by fluid flow. The stochastic modelling sequence is applied to a large, steady-state 3-D heat flow model of a reservoir in The Hague, Netherlands. The spatial thermal conductivity distribution is simulated stochastically based on available logging data. Errors of bottom-hole temperatures provide thresholds for the constraining technique performed afterwards. This reduce the temperature uncertainty for the proposed target location significantly from 25 to 12 K (full distribution width) in a depth of 2300 m. Assuming a Gaussian shape of the temperature distribution, the standard deviation is 1.8 K. To allow a more comprehensive approach to quantify uncertainty, we also implement the stochastic simulation of boundary conditions and demonstrate this for the basal specific heat flow in the reservoir of The Hague. As expected, this results in a larger distribution width and hence, a larger, but more realistic uncertainty estimate. However, applying the constraining post-processing the uncertainty is again reduced to the level of the post-processing without stochastic boundary simulation. Thus, constraining post-processing is a suitable tool for reducing uncertainty estimates by observed state variables. Y1 - 2010 U6 - https://doi.org/10.1111/j.1365-246X.2009.04498.x SN - 1365-246X VL - 181 IS - 1 SP - 321 EP - 333 PB - Oxford University Press CY - Oxford ER - TY - CHAP A1 - Kirsch, Ansgar T1 - Numerical investigation of the face stability of shallow tunnels in sand T2 - Numerical methods in geotechnical engineering : (NUMGE 2010) : proceedings of the seventh European Conference on Numerical Methods in Geotechnical Engineering, Trondheim, Norway, 2 - 4 June 2010 / ed. by Thomas Benz ... Y1 - 2010 SN - 978-0-415-59239-0 SP - 779 EP - 784 PB - Taylor and Francis CY - London ER - TY - CHAP A1 - Kirsch, Ansgar A1 - Marcher, Thomas T1 - Numerical prediction of time-dependent rock swelling based on an example of a major tunnel project in Ontario/Canada T2 - Numerical methods in geotechnical engineering : (NUMGE 2010) : proceedings of the seventh European Conference on Numerical Methods in Geotechnical Engineering, Trondheim, Norway, 2 - 4 June 2010 / ed. by Thomas Benz ... Y1 - 2010 SN - 978-0-415-59239-0 SP - 297 EP - 302 PB - Taylor and Francis CY - London ER - TY - PAT A1 - O'Connell, Timothy A1 - Siegert, Petra A1 - Maurer, Karl-Heinz A1 - Schiedel, Marc-Steffen A1 - Vockenroth, Inga Kerstin T1 - Method for improving the cleaning action of a detergent or cleaning agent [Internationale Patentanmeldung] T1 - Verfahren zur Verbesserung der Reinigungsleistung eines Wasch- oder Reinigungsmittels Y1 - 2010 SP - 1 EP - 15 PB - WIPO CY - Genf ER - TY - CHAP A1 - Gömmel, Andreas A1 - Niendorf, Thoralf A1 - Frauenrath, Tobias A1 - Otten, Mario A1 - Butenweg, Christoph A1 - Kob, Malte T1 - 3D vocal fold geometry mapping using Magnetic Resonance Imaging T2 - Fortschritte der Akustik : 36. Deutsche Jahrestagung für Akustik, Band 1 Y1 - 2010 SN - 978-3-9808659-8-2 N1 - Fortschritte der Akustik : 36. Deutsche Jahrestagung für Akustik ; 15.-18. März 2010, Berlin SP - 271 EP - 272 PB - Deutsche Gesellschaft für Akustik CY - Berlin ER -