TY  - JOUR
A1  - Falkenberg, Fabian
A1  - Bott, Michael
A1  - Bongaerts, Johannes
A1  - Siegert, Petra
T1  - Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
JF  - Frontiers in Microbiology
N2  - The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates.
Y1  - 2022
U6  - http://dx.doi.org/10.3389/fmicb.2022.1017978
SN  - 1664-302X
VL  - 2022
IS  - 13
PB  - Frontiers
CY  - Lausanne
ER  - 
TY  - BOOK
A1  - Lauth, Jakob
T1  - Physical chemistry in a nutshell: Basics for engineers and scientists
N2  - This book is based on a multimedia course for biological and chemical engineers, which is designed to trigger students' curiosity and initiative. A solid basic knowledge of thermodynamics and kinetics is necessary for understanding many technical, chemical, and biological processes.
The one-semester basic lecture course was divided into 12 workshops (chapters). Each chapter covers a practically relevant area of physical chemistry and contains the following didactic elements that make this book particularly exciting and understandable:
- Links to Videos at the start of each chapter as preparation for the workshop
- Key terms (in bold) for further research of your own
- Comprehension questions and calculation exercises with solutions as learning checks
- Key illustrations as simple, easy-to-replicate blackboard pictures
Humorous cartoons for each workshop (by Faelis) additionally lighten up the text and facilitate the learning process as a mnemonic. To round out the book, the appendix includes a summary of the most popular experiments in basic physical chemistry courses, as well as suggestions for designing workshops with exhibits, experiments, and "questions of the day."
Suitable for students minoring in chemistry; chemistry majors are sure to find this slimmed-down, didactically valuable book helpful as well. The book is excellent for self-study.
KW  - Physical chemistry
KW  - Thermodynamics as minor
KW  - Physical chemistry starters
KW  - Minor chemistry
KW  - Physical chemistry basics
Y1  - 2023
SN  - 978-3-662-67636-3 (Softcover)
SN  - 978-3-662-67637-0 (eBook)
U6  - http://dx.doi.org/10.1007/978-3-662-67637-0
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Aggarwal, Prerna
A1  - Singh, Virendra
A1  - Singh, Arjun
A1  - Scherer, Ulrich W.
A1  - Singh, Tejvir
A1  - Singla, Madan L.
A1  - Srivastava, Alok
T1  - Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate)
JF  - Radiation Physics and Chemistry
Y1  - 2011
SN  - 0969-806X
N1  - In Press, Accepted Manuscript
SP  - 1
EP  - 28
PB  - Pergamon Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Smith, Walker O.
A1  - Baumann, Marcus
A1  - Wilson, David L.
A1  - Aletsee, Ludwig
T1  - Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984
JF  - Journal of geophysical research
Y1  - 1987
SN  - 2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print)
VL  - Vol. 92
IS  - Iss. C7
SP  - 6777
EP  - 6786
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Goeyens, L.
A1  - Semeneh, M.
A1  - Elskens, M.
T1  - Phytoplankton nutrient utilisation and nutrient signature in the Southern Ocean / Goeyens, L. ; Semeneh, M. ; Baumann, M.E.M. ; Elskens, M. ; Shopova, D. ; Dehairs, F.
JF  - Journal of Marine Systems. 17 (1998), H. 1-4
Y1  - 1998
SN  - 0924-7963
SP  - 143
EP  - 157
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Zeng, Steffen
A1  - Dinter, Andre
A1  - Eisenkrätzer, Detlef
T1  - Pilot Scale Expression and Purification of Soluble Protein A Tagged β1,6N-Acetylglucosaminyltransferase in CHO Cells / Zeng, Steffen ; Dinter, Andre ; Eisenkrätzer, Detlef ; Biselli, Manfred ; Wandrey, Christian ; Berger, Eric G.
JF  - Biochemical and Biophysical Research Communications. 237 (1997), H. 3
Y1  - 1997
SN  - 0006-291X
SP  - 653
EP  - 658
ER  - 
TY  - JOUR
A1  - Baumann, Marcus
A1  - Hirche, H.-J.
A1  - Kattner, G.
A1  - Gradinger, R.
T1  - Plankton distribution and the impact of copepod grazing on primary production in Fram Strait, Greenland Sea / Hirche, H.-J. ; Baumann, M.E.M. ; Kattner, G.; Gradinger, R.
JF  - Journal of Marine Systems. 2 (1991), H. 3-4
Y1  - 1991
SN  - 0924-7963
SP  - 477
EP  - 494
ER  - 
TY  - JOUR
A1  - Michalak, Ewa Malgorzata
A1  - Nacerddine, Karim
A1  - Pietersen, Alexandra
A1  - Beuger, Vincent
A1  - Pawlitzky, Inka
A1  - Cornelissen-Steijger, Paulien
A1  - Wientjens, Ellen
A1  - Tanger, Ellen
A1  - Seibler, Jost
A1  - Lohuizen, Maarten van
A1  - Jonkers, Jos
T1  - Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool
JF  - Stem Cells
Y1  - 2013
U6  - http://dx.doi.org/10.1002/stem.1437
SN  - 1549-4918
VL  - Vol 31
IS  - 9
SP  - 1910
EP  - 1920
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kricheldorf, Hans R.
A1  - Jonté, J. Michael
T1  - Polylactones, 2. Copolymerization of glycolide with β-propiolactone,  χ-butyrolactone or δ-valerolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jonté, J. Michael
JF  - Die makromolekulare Chemie. 186 (1985), H. 5
Y1  - 1985
SN  - 1022-1352
SP  - 955
EP  - 976
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kricheldorf, Hans R.
A1  - Jonté, J. Michael
T1  - Polylactones. 1. Copolymerizations of glycolide and &beta-caprolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jonté, J. Michael
JF  - Macromolecules. 17 (1984), H. 10
Y1  - 1984
SN  - 0024-9297
SP  - 2173
EP  - 2181
ER  - 
TY  - JOUR
A1  - Mang, Thomas
T1  - Polymer Magnetic Particles for Isolating Biomolecules, Especially Nucleic Acids
JF  - Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2
Y1  - 2004
SN  - 0013-5585
SP  - 1008
EP  - 1009
ER  - 
TY  - JOUR
A1  - Oosterhuis, Koen
A1  - Öhlschläger, Peter
A1  - Berg, Joost H. van den
A1  - Toebes, Mireille
A1  - Gomez, Raquel
A1  - Schumacher, Ton N.
A1  - Haanen, John B.
T1  - Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7
JF  - International Journal of Cancer
Y1  - 2011
SN  - 1097-0215
VL  - 129
IS  - 2
SP  - 397
EP  - 406
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Henken, F. E.
A1  - Oosterhuis, K.
A1  - Öhlschläger, Peter
A1  - Bosch, L.
A1  - Hooijberg, E.
A1  - Haanen, J. B. A. G.
A1  - Steenbergen, R. D. M.
T1  - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7
JF  - Vaccine
N2  - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.vaccine.2012.04.013
SN  - 0264-410X
VL  - 30
IS  - 28
SP  - 4259
EP  - 4266
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - BOOK
A1  - Lauth, Jakob
A1  - Harreus, Albrecht
A1  - Götz, Norbert
A1  - Rang, Harald [u.a.]
T1  - Preparation O-(2-Hydroxyalkyl)Oximes = of Verfahren zur Herstellung von O-(2-Hydroxy)Oximen : Europäische Patentschrift EP0775107B1 ; Veröffentlichungstag im Patentblatt: 18.11.1999 / Anmelder: BASF AG, 67063 Ludwigshafen. Erfinder: Albrecht Harreus ; Norbert Götz ; Harald Rang ... Günter Lauth
Y1  - 1999
N1  - Volltext über Datenbank: http://publikationen.dpma.de/ ; Text in engl. ; Deutsche Übersetzung unter DE69512786T2 ; Internationale Veröffentlichungsnummer: WO 96/04238
PB  - Europäisches Patentamt
CY  - [München u.a.]
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Wolf, Gerhard
A1  - Burkhart, Bernd
T1  - Preparation of alkoxylation products in the presence of mixed hydroxides modified with additives : United States Patent ; patent number 5,741,947 ; date of patent: Apr. 21, 1998 / assignee: BASF Aktiengesellschaft. Inventors: Gerhard Wolf ; Bernd Burkhart ; Guenter Lauth ...
Y1  - 1998
N1  - Barcode: US005741947A ; Volltext über Datenbank: http://www.google.com/patents
PB  - [United States Trademark and Patent Office]
CY  - [Alexandria, VA]
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Müller, Ulrich
A1  - Hoelderich, Wolfgang
T1  - Preparation of aluminophosphates and silicoaluminophosphates having the AEL structure using 1,2-bis-( 4-pyridyl)-ethane : United States Patent ; patent number 5,324,493 ; date of patent: Jun. 28, 1994 / assignee: BASF Aktiengesellschaft. Inventors: Ulrich Mueller ; Wolfgang Hoelderich ; Guenter Lauth
Y1  - 1994
N1  - Barcode: US005324493A ; Volltext über Datenbank: http://www.google.com/patents
PB  - [United States Trademark and Patent Office]
CY  - [Alexandria, VA]
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Dingerdissen, Uwe
A1  - Henne, Andreas
T1  - Preparation of amines  : United States Patent ; patent number 5,817,871 ; date of patent: Oct. 6, 1998 / Assignee: BASF Aktiengesellschaft. Inventors: Uwe Dingerdissen ; Gunter Lauth ; Andreas Henne ....
Y1  - 1998
N1  - Barcode: US005817871A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung: Gunter ...
PB  - [United States Trademark and Patent Office]
CY  - [Alexandria, VA]
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Dingerdissen, Uwe
A1  - Trübenbach, Peter
A1  - Steuerle, Ulrich
T1  - Preparation of aziridines : United States Patent ; patent number 5,693,825 ; date of patent: Dec. 2, 1997 / assignee: BASF Aktiengesellschaft. Inventors: Uwe Dingerdissen ; Gunter Lauth ; Peter Trubenbach ; Ulrich Steuerle
Y1  - 1997
N1  - Barcode: US005324493A ; Volltext über Datenbank: http://www.google.com/patents ; Falsche Namensansetzung in Datenbank: Gunter ...
PB  - United States Patent and Trademark Office
CY  - [Alexandria, VA]
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Schmid, Georg
A1  - Wandrey, Christian
T1  - Preparation of cellodextrins and isolation of oligomeric side components and their characterization / Schmid, Georg ; Biselli, Manfred ; Wandrey, Christian
JF  - Analytical Biochemistry. 175 (1988)
Y1  - 1988
SN  - 0003-2697
SP  - 573
EP  - 583
ER  - 
TY  - PAT
A1  - Lauth, Jakob
A1  - Harreus, Albrecht
A1  - Goetz, Norbert
A1  - Rang, Harald
T1  - Preparation of O-(2-hydroxyalkyl) oximes : [South African Patent ] publication number: ZA9506402 ; publication date: 1997-02-03 / applicant: BASF AG.  Inventor: Harreus, Albrecht ; Goetz, Norbert ; Rang, Harald .... Lauth, Guenter ...
Y1  - 1997
N1  - Also published as WO9604238
PB  - [South African Companies and Intellectual Property Registration Office]
CY  - [Pretoria]
ER  - 
TY  - PAT
A1  - Lauth, Jakob
T1  - Preparation of O-(2-hydroxyalkyl) oximes : patent application CA 2196452 ; filing date: 1995-07-28 ; national entry: 1997-01-30 / applicants: BASF Aktiengesellschaft. Inventors: Harreus, Albrecht ; Gotz, Norbert ; Rang, Harald ... Lauth, Guenther ...
Y1  - 1995
N1  - International publication number: WO1996/004238
PB  - Canadian International Property Office
CY  - [Gatineau, Quebec]
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Hammon, U.
T1  - Preparation of pellets with well-defined pore structure / U. Hammon ; A. Kotter
JF  - International chemical engineering. 26 (1986), H. 4
Y1  - 1986
SN  - 0020-6318
SP  - 563
EP  - 573
ER  - 
TY  - JOUR
A1  - Naithani, V. K
A1  - Klostermeyer, Henning
A1  - Lange, H. R.
A1  - [u.a.], 
A1  - Berndt, Heinz
A1  - [u.a.], 
T1  - Preparation of peptide derivatives for porcine proinsulin-synthesis
JF  - Biological Chemistry
Y1  - 1971
U6  - http://dx.doi.org/10.1515/bchm2.1971.352.1.1
SN  - 1437-4315
VL  - 352
IS  - 1
SP  - 2
EP  - 3
PB  - De Gruyter
ER  - 
TY  - INPR
A1  - Greiner, Lasse
A1  - Jeromin, Günter Erich
A1  - Sithole, Patience
A1  - Petersen, Soenke
T1  - Preprint: Studies on the enzymatic reduction of levulinic acid using Chiralidon-R and Chiralidon-S
T2  - ChemRxiv
N2  - The enzymatic reduction of levulinic acid by the chiral catalysts Chiralidon-R and Chiralidon-S which are commercially available superabsorbed alcohol dehydrogenases is described. The Chiralidon®-R/S reduces the levulinic acid to the (R,S)-4-hydroxy valeric acid and the (R)- or (S)- gamma-valerolactone.
KW  - Levulinic acid
KW  - Chiralidon-R
KW  - Chiralidon-S
KW  - 4-hydroxy valeric acid
KW  - (R)- or (S)- gamma-valerolactone
Y1  - 2023
U6  - http://dx.doi.org/10.26434/chemrxiv-2023-jlvcv
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Woznyj, M.
A1  - Lüdemann, H.-D.
T1  - Pressure Dependence of the Melting and Self Diffusion in 2,2-Dimethylpropane, 2,2-Dimethylpropionitrile, and 2-Methylpropanol-2 / M. Woznyj, F. X. Prielmeier, H.-D. Lüdemann
JF  - Zeitschrift für Naturforschung A, Journal of Physical Sciences. 39 (1984)
Y1  - 1984
SN  - 0932-0784
SP  - 800
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Lang, E. W.
A1  - Lüdemann, H.-D.
T1  - Pressure dependence of the self-diffusion in liquid trifluoromethane / F. X. Prielmeier; E. W. Lang; H.-D. Lüdemann
JF  - Molecular Physics. 52 (1984), H. 5
Y1  - 1984
SN  - 0026-8976
SP  - 1105
EP  - 1113
ER  - 
TY  - CHAP
A1  - Möhring, S.
A1  - Wulfhorst, H.
A1  - Roth, J.
A1  - Tippkötter, Nils
T1  - Pretreatment strategies for lignocellulosic biomass
T2  - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany
Y1  - 2016
SP  - 131
PB  - DECHEMA
CY  - Frankfurt am Main
ER  - 
TY  - CHAP
A1  - Baumann, Marcus
ED  - Hempel, G.
T1  - Primary production and phytoplankton growth in the marginal ice zone of the Fram Strait and the interpretation of the field data with autecological experiments (Abstract)
T2  - Oceanography and biology of Arctic seas : selected papers from the ICES Symposium on Marine Sciences of the Arctic and Subarctic Regions, held in Santander, 28 - 30 September 1987. (Rapports et procès-verbaux des réunions / Conseil International pour l'Exploration de la Mer ; 188)
Y1  - 1989
SP  - 128
PB  - International Council for the Exploration of the Sea
CY  - Copenhagen
ER  - 
TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements
JF  - Geophysical research letters
Y1  - 1994
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 21
IS  - Iss. 8
SP  - 689
EP  - 692
ER  - 
TY  - JOUR
A1  - Gerigk, M.
A1  - Bujnicki, R.
A1  - Ganpo-Nkwenkwa, E.
A1  - Bongaerts, Johannes
A1  - Sprenger, G.
A1  - Takors, Ralf
T1  - Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains
JF  - Biotechnology and bioengineering
Y1  - 2002
SN  - 1097-0290 (E-Journal); 0006-3592 (Print)
VL  - Vol. 80
IS  - Iss. 7
SP  - 746
EP  - 754
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Born, C.
A1  - Wandrey, C.
T1  - Production of monoclonal antibodies in a pilot scale fluidized bed bioreactor / Born, C.; Biselli, M.; Wandrey, C.
JF  - Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery
Y1  - 1995
SN  - 0-7923-3736-0
N1  - Meeting "Animal Cell Technology, Developments towards the 21st Century" ; <1994.09.12-16 : ; Veldhoven>
SP  - 515
EP  - 519
PB  - Kluwer Acad. Press
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Hoehr, Cornelia
A1  - Hou, Xinchi
A1  - Hanemaayer, Victoire
A1  - Zeisler, Stefan
A1  - Adam, Michael J.
A1  - Ruth, Thomas J.
A1  - Celler, Anna
A1  - Buckley, Ken
A1  - Benard, Francois
A1  - Schaffer, Paul
T1  - Production of Y-86 and other radiometals for research purposes using a solution target system
JF  - Nuclear medicine and biology
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.nucmedbio.2015.06.005
SN  - 1872-9614
VL  - 42
IS  - 11
SP  - 842
EP  - 849
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Berndt, Heinz
A1  - Kalbe, Jochen
A1  - Kuropka, Rolf
A1  - Meyer-Stork, L. Sebastian
A1  - Höcker, Hartwig
ED  - Körner, Andrea
T1  - Progress and limitations of the DNA analysis in fine animal fiber identification
T2  - Proceedings of the 2nd International Symposium on Specialty Animal Fibers : Aachen, October 19 - 20, 1989. - (Schriftenreihe des Deutschen Wollforschungsinstituts an der Technischen Hochschule Aachen e. V. ; 106)
Y1  - 1990
SP  - 259
EP  - 265
PB  - Dt. Wollforschungsinst.
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Achebach, Stephanie
A1  - Unden, Gottfried
T1  - Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]·FNR of Escherichia coli / Achebach, S. ; Selmer, T. ; Unden, G.
JF  - The FEBS Journal. 272 (2005), H. 16
Y1  - 2005
SN  - 1742-464X
SP  - 4260
EP  - 4269
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Willanzheimer, Angela
A1  - Hetzel, Marc
T1  - Propionate CoA-transferase from Clostridium propionicum. Cloning of the gene and identification of glutamate 324 at the active site
JF  - European Journal of Biochemistry. 269 (2002), H. 1
Y1  - 2002
SN  - 0014-2956
SP  - 372
EP  - 380
ER  - 
TY  - JOUR
A1  - Dellmann, Sophia Florence
A1  - Glorius, J.
A1  - Litvinov, Yu A.
A1  - Reifarth, R.
A1  - Al-Khasawneh, Kafa
A1  - Aliotta, M.
A1  - Bott, L.
A1  - Brückner, Benjamin
A1  - Bruno, C. G.
A1  - Chen, Ruijiu
A1  - Davinson, T.
A1  - Dickel, T.
A1  - Dillmann, Iris
A1  - Dmytriev, D.
A1  - Erbacher, P.
A1  - Freire-Fernández, D.
A1  - Forstner, Oliver
A1  - Geissel, H.
A1  - Göbel, K.
A1  - Griffin, Christopher J.
A1  - Grisenti, R.
A1  - Gumberidze, Alexandre
A1  - Haettner, Emma
A1  - Hagmann, Siegbert
A1  - Heil, M.
A1  - Heß, R.
A1  - Hillenbrand, P.-M.
A1  - Joseph, R.
A1  - Jurado, B.
A1  - Kozhuharov, Christophor
A1  - Kulikov, I.
A1  - Löher, Bastian
A1  - Langer, Christoph
A1  - Leckenby, Guy
A1  - Lederer-Woods, C.
A1  - Lestinsky, M.
A1  - Litvinov, S. A.
A1  - Lorenz, B. A.
A1  - Lorenz, E.
A1  - Marsh, J.
A1  - Menz, Esther Babette
A1  - Morgenroth, T.
A1  - Petridis, N.
A1  - Pibernat, Jerome
A1  - Popp, U.
A1  - Psaltis, Athanasios
A1  - Sanjari, Shahab
A1  - Scheidenberger, C.
A1  - Sguazzin, M.
A1  - Sidhu, Ragandeep Singh
A1  - Spillmann, Uwe
A1  - Steck, M.
A1  - Stöhlker, T.
A1  - Surzhykov, A.
A1  - Swartz, J. A.
A1  - Törnqvist, H.
A1  - Varga, L.
A1  - Vescovi, Diego
A1  - Weick, H.
A1  - Weigand, M.
A1  - Woods, P.
A1  - Xing, Y.
A1  - Yamaguchi, Taiyo
T1  - Proton capture on stored radioactive ¹¹⁸Te ions
JF  - EPJ Web of Conferences
N2  - Experimental determination of the cross sections of proton capture on radioactive nuclei is extremely difficult. Therefore, it is of substantial interest for the understanding of the production of the p-nuclei. For the first time, a direct measurement of proton-capture cross sections on stored, radioactive ions became possible in an energy range of interest for nuclear astrophysics. The experiment was performed at the Experimental Storage Ring (ESR) at GSI by making use of a sensitive method to measure (p,γ) and (p,n) reactions in inverse kinematics. These reaction channels are of high relevance for the nucleosyn-thesis processes in supernovae, which are among the most violent explosions in the universe and are not yet well understood. The cross section of the ¹¹⁸Te(p,γ) reaction has been measured at energies of 6 MeV/u and 7 MeV/u. The heavy ions interacted with a hydrogen gas jet target. The radiative recombination process of the fully stripped ¹¹⁸Te ions and electrons from the hydrogen target was used as a luminosity monitor. An overview of the experimental method and preliminary results from the ongoing analysis will be presented.
Y1  - 2023
U6  - http://dx.doi.org/10.1051/epjconf/202327911018
SN  - 2100-014X
N1  - Volume 279, 2023. Nuclear Physics in Astrophysics – X (NPA-X 2022).
VL  - 279
IS  - Article Number: 11018
SP  - 1
EP  - 5
PB  - EDP Sciences
ER  - 
TY  - JOUR
A1  - Schmitz, M.
A1  - Hirsch, E.
A1  - Bongaerts, Johannes
A1  - Takors, Ralf
T1  - Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli
JF  - Biotechnology progress
Y1  - 2002
SN  - 1520-6033 (E-Journal); 8756-7938 (Print)
VL  - Vol. 18
IS  - Iss. 5
SP  - 935
EP  - 941
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Thömmes, J.
A1  - Born, C.
A1  - Wandrey, C.
T1  - Purification of monoclonal antibodies by fluidized bed adsorption / Thömmes, J. ; Born, C.  Biselli, M. ; Wandrey, C. ; Kula, M.-R.
JF  - Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery
Y1  - 1995
SN  - 0-7923-3736-0
N1  - Meeting "Animal Cell Technology, Developments towards the 21st Century" ; <1994.09.12-16 : ; Veldhoven>
SP  - 515
EP  - 519
PB  - Kluwer Acad. Press
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Hasegawa, Maki
A1  - Kapelyukh, Yury
A1  - Tahara, Harunobu
A1  - Seibler, Jost
A1  - Rode, Anja
A1  - Krueger, Sylvia
A1  - Lee, Dongtao N.
A1  - Wolf, C. Roland
A1  - Scheer, Nico
T1  - Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line
JF  - Molecular Pharmacology
Y1  - 2011
U6  - http://dx.doi.org/10.1124/mol.111.071845
SN  - 1521-0111
VL  - 80
IS  - 33
SP  - 518
EP  - 528
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Monakhova, Yulia
A1  - Soboleva, Polina M.
A1  - Fedotova, Elena S.
A1  - Musina, Kristina T.
A1  - Burmistrova, Natalia A.
T1  - Quantum chemical calculations of IR spectra of heparin disaccharide subunits
JF  - Computational and Theoretical Chemistry
N2  - Heparin is a natural polysaccharide, which plays essential role in many biological processes. Alterations in building blocks can modify biological roles of commercial heparin products, due to significant changes in the conformation of the polymer chain. The variability structure of heparin leads to difficulty in quality control using different analytical methods, including infrared (IR) spectroscopy. In this paper molecular modelling of heparin disaccharide subunits was performed using quantum chemistry. The structural and spectral parameters of these disaccharides have been calculated using RHF/6-311G. In addition, over-sulphated chondroitin sulphate disaccharide was studied as one of the most widespread contaminants of heparin. Calculated IR spectra were analyzed with respect to specific structure parameters. IR spectroscopic fingerprint was found to be sensitive to substitution pattern of disaccharide subunits. Vibrational assignments of calculated spectra were correlated with experimental IR spectral bands of native heparin. Chemometrics was used to perform multivariate analysis of simulated spectral data.
KW  - IR spectroscopy
KW  - Chemometrics
KW  - Quantum chemistry
KW  - Molecular modelling
KW  - Quality control
Y1  - 2022
SN  - 2210-271X
U6  - http://dx.doi.org/10.1016/j.comptc.2022.113891
VL  - 1217
IS  - Article number: 113891
PB  - Elsevier
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Lassonczyk, Beate
A1  - Voigt, B.
A1  - Gabriel, B.
A1  - Ghod, Mumin M.
T1  - Quaternary events  at the Horn of Africa / Voigt, B., B. Gabriel,  B. Lassonczyk and Mumin M. Ghod
JF  - Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Paläontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universität Berlin. 120 A (1990)
Y1  - 1990
SN  - 0172-8784
SP  - 679
EP  - 694
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Tomasberger, T.
A1  - Veltkamp, T. C.
A1  - Booij, A. S.
T1  - Radiocesium Removal from High Level Liquid Waste and Immobilisation in Sodium SilicoTitanate for Geological Disposal / T. Tomasberger, T.C. Veltkamp, A.S. Booij, U.W. Scherer
JF  - Radiochimica Acta. 89 (2001), H. 3
Y1  - 2001
SN  - 0033-8230
SP  - 145
EP  - 150
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Zevnik, Branko
A1  - Küter-Luks, Birgit
A1  - Andreas, Susanne
A1  - Kern, Heidrun
A1  - Hennek, Thomas
A1  - Rode, Anja
A1  - Heimann, Cornelia
A1  - Faust, Nicole
A1  - Kauselmann, Gunther
A1  - Schoor, Michael
A1  - Jaenisch, Rudolf
A1  - Rajewsky, Klaus
A1  - Kühn, Ralf
A1  - Schwenk, Frieder
T1  - Rapid generation of inducible mouse mutants
JF  - Nucleic Acids Research
Y1  - 2003
U6  - http://dx.doi.org/10.1093/nar/gng012
SN  - 1362-4962
VL  - 33
IS  - 4
SP  - e12
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Kragl, U.
A1  - Wandrey, C.
T1  - Reaction Engineering for Enzyme-Catalyzed Biotransformations
JF  - Enzyme catalysis in organic synthesis : a comprehensive handbook. Bd. 1. -  2., completely rev. and enl. ed. / ed. by Karlheinz Drauz.
Y1  - 2002
SN  - 3-527-29949-1
N1  - erschienen: Vol. 1-3
SP  - 185
EP  - 257
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Kragl, U.
A1  - Wandrey, C.
T1  - Reaction Engineering for Enzyme-Catalyzed Biotransformations
JF  - Enzyme catalysis in organic synthesis : a comprehensive handbook. Bd. 1.  / ed. by Karlheinz Drauz.
Y1  - 1995
SN  - 3-527-28479-6
N1  - erschienen: Vol. 1-2
SP  - 89
EP  - 155
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Biselli, Manfred
A1  - Wandrey, Christian
T1  - Reaction Mechanism of an Endoglucanase of Trichoderma Reesei QM 9414 using Cellodextrins as Substrate
JF  - Technology of biological processes, safety in biotechnology, applied genetic engineering : lectures held at the 5th DECHEMA Annual Meeting of Biotechnologists, May 12 - 13, 1987, [Frankfurt, Main] / [hrsg. von Dieter Behrens ...]
Y1  - 1988
N1  - Jahrestagung der Biotechnologen ; <5, 1987, Frankfurt, Main> ; DECHEMA Biotechnology Conferences series ; 1
SP  - 425
EP  - 429
PB  - VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Scherer, Ulrich W.
A1  - Brüchle, W.
A1  - Brügger, M.
A1  - Frink, C.
T1  - Reactions of 40Ar with 233U,,235U, and 238U at the Barrier / U.W. Scherer, W. Brüchle, M. Brügger, C. Frink, H. Gäggeler, G. Herrmann, J.V. Kratz, K.J. Moody, M. Schädel, K. Sümmerer, N. Trautmann, G. Wirth
JF  - Zeitschrift für Physik A Hadrons and Nuclei. 335 (1990), H. 4
Y1  - 1990
SN  - 0939-7922
SP  - 421
EP  - 430
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Schweighöfer, Philip V.
A1  - Abram, Ulrich
T1  - Reactions of [ReOX3(PPh3)2] Complexes (X = Cl, Br) with Phenylacetylene and the Structures of the Products
JF  - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry
N2  - Oxorhenium(V) complexes [ReOX3(PPh3)2] (X = Cl, Br) react with phenylacetylene under formation of complexes with ylide-type ligands. Compounds of the compositions [ReOCl3(PPh3){C(Ph)C(H)(PPh3)}] (1), [ReOBr3(OPPh3){C(Ph)C(H)(PPh3)}] (2), and [ReOBr3(OPPh3){C(H)C(Ph)(PPh3)}] (3) were isolated and characterized by X-ray diffraction. They contain a ligand, which was formed by a nucleophilic attack of released PPh3 at coordinated phenylacetylene. The structures of the products show that there is no preferable position for this attack. Cleavage of the Re–C bond in 3 and dimerization of the organic ligand resulted in the formation of the [{(PPh3)(H)CC(Ph)}2]2+ cation, which crystallized as its [(ReOBr4)(OReO3)]2– salt.
Y1  - 2010
U6  - http://dx.doi.org/10.1002/zaac.200900478
SN  - 1521-3749
VL  - 636
IS  - 5
SP  - 779
EP  - 783
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Haulena, Friedhelm
T1  - Recycling of Polymeric Composites
JF  - Invited and contributing lectures presented at the 38th Microsymposium on Recycling of Polymers : held in Prague, Czech Republic, July 14 - 17, 1997 / International Union of Pure and Applied Chemistry, Macromolecular Division. Symposium ed. J. Kahovec.
Y1  - 1998
N1  - Macromolecular symposia ; 135 ; Microsymposium on Recycling of Polymers <38, 1997, Praha>
SP  - 147
EP  - 156
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Tippkötter, Nils
A1  - Wollny, Steffen
A1  - Suck, Kirstin
A1  - Sohling, Ulrich
A1  - Ruf, Friedrich
A1  - Ulber, Roland
T1  - Recycling of spent oil bleaching earth as source of glycerol for the anaerobic production of acetone, butanol, and ethanol with Clostridium diolis and lipolytic Clostridium lundense
JF  - Engineering in Life Sciences
N2  - A major part of edible oil is subjected to bleaching procedures, primarily with minerals applied as adsorbers. Their recycling is currently done either by regaining the oil via organic solvent extraction or by using the spent bleaching earth (SBE) as additive for animal feed, etc. As a new method, the reutilization of the by-product SBE for the microbiologic formation of acetone, butanol, and ethanol (ABE) is presented as proof-of-concept. The SBE was taken from a palm oil cleaning process. The recycling concept is based on the application of lipolytic clostridia strains. Due to considerably long fermentation times, co-fermentation with Candida rugosa and enzymatic hydrolyses of the bound oil with a subsequent clostridia fermentation are shown as alternative routes. Anaerobic fermentations under comparison of different clostridia strains were performed with glycerol media, enzymatically hydrolyzed palm oil and SBE. Solutes, side product compositions and productivities were quantified via HPLC. A successful production of ABE solutes from SBE has been done with a yield of 0.15 g butanol per gram of bound glycerol. Thus, the biotechnological recycling of the waste stream is possible in principle. Inhibition of the substrate suspension has been observed. A chromatographic ion-exchange of substrates increased the biomass concentration.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/elsc.201300113
SN  - 1618-2863
VL  - 14
IS  - 4
SP  - 425
EP  - 432
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Tüg, Helmut
A1  - Baumann, Marcus
T1  - Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper “Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements”
JF  - Geophysical research letters
Y1  - 1995
SN  - 1944-8007 (E-Journal); 0094-8276 (Print)
VL  - Vol. 22
IS  - Iss. 9
SP  - 1159
EP  - 1160
ER  - 
TY  - JOUR
A1  - Tran, Quang Hon
A1  - Bongaerts, Johannes
A1  - Vlad, Dorina
A1  - Unden, Gottfried
T1  - Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications
JF  - European journal of biochemistry
Y1  - 1997
SN  - 0014-2956
VL  - Vol. 244
IS  - Iss. 1
SP  - 155
EP  - 160
ER  - 
TY  - JOUR
A1  - Berndt, Heinz
A1  - Krüger, Götz
T1  - Resolution of enantiomeric amino acid derivatives by high-performance liquid chromatography on chiral stationary phases
JF  - Journal of chromatography A
Y1  - 1985
U6  - http://dx.doi.org/10.1016/S0021-9673(01)92461-6
SN  - 0021-9673
VL  - 1985
IS  - 348
SP  - 275
EP  - 279
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Kleinridders, Andre
A1  - Küter-Luks, Birgit
A1  - Niehaves, Sandra
A1  - Brüning, Jens C.
A1  - Schwenk, Frieder
T1  - Reversible gene knockdown in mice using a tight, inducible shRNA expression system
JF  - Nucleic Acids Research
Y1  - 2007
U6  - http://dx.doi.org/10.1093/nar/gkm122
SN  - 1362-4962
VL  - 35
IS  - 7
SP  - e54
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Dietrich, Sascha
A1  - Hertel, Robert
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Volland, Sonja
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation
JF  - BMC genomics
Y1  - 2013
SN  - 1471-2164
VL  - Vol. 14
SP  - 667
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Scheele, Sandra
A1  - Oertel, Dan
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
A1  - Bott, Michael
A1  - Freudl, Roland
T1  - Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum
JF  - Microbial biotechnology
Y1  - 2013
SN  - 1751-7915
SP  - 202
EP  - 206
PB  - Wiley-Blackwell
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Lintz, Hans-Günther
A1  - Turek, Thomas
T1  - Selective catalytic reduction of nitrogen oxide by use of the Ljungstroem air heater as reactor : a case study
JF  - Chemical engineering science  . 47 (1992), H. 9-11
Y1  - 1992
SN  - 0009-2509
SP  - 2763
EP  - 2768
ER  - 
TY  - JOUR
A1  - Prielmeier, Franz
A1  - Lüdemann, H.-D.
T1  - Self diffusion in compressed liquid chloromethane, dichloromethane and trichloromethane / F. X. Prielmeier; H.-D. Lüdemann
JF  - Molecular Physics. 58 (1986), H. 3
Y1  - 1986
SN  - 0026-8976
SP  - 593
EP  - 604
ER  - 
TY  - JOUR
A1  - Nachtrodt, Frederik
A1  - Tietsch, Wolfgang
A1  - Mostacci, Domiziano
A1  - Scherer, Ulrich W.
T1  - Set-up and first operation of a plasma oven for treatment of low level radioactive wastes
JF  - Nuclear technology and radiation protection
Y1  - 2014
U6  - http://dx.doi.org/10.2298/NTRP140SS47N
SN  - 1451-3994
VL  - 29
IS  - Suppl.
SP  - 47
EP  - 51
PB  - VINČA Institute of Nuclear Sciences
CY  - Belgrad
ER  - 
TY  - PAT
A1  - Berndt, Heinz
A1  - Höcker, Hartwig
A1  - Kuropka, Rolf
A1  - Kinkel, Joachim
T1  - Silane coated inorganic materials for chromatography : United States Patent
Y1  - 1991
PB  - [The United States Patent and Trademark Office]
CY  - [Alexandria, VA u.a.]
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Kückmann, Theresa
A1  - Abram, Ulrich
T1  - Silver(I) Complexes of 1,3-Dialkyl-4,5-dimethylimidazol-2-ylidenes and their Use as Precursors for the Synthesis of Rhenium(V) NHC Complexes
JF  - Zeitschrift für anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry
Y1  - 2007
U6  - http://dx.doi.org/10.1002/zaac.200700021
SN  - 1521-3749
VL  - 633
IS  - 5-6
SP  - 830
EP  - 834
ER  - 
TY  - JOUR
A1  - Monakhova, Yulia
A1  - Diehl, Bernd W. K.
T1  - Simplification of NMR Workflows by Standardization Using 2H Integral of Deuterated Solvent as Applied to Aloe vera Preparations
JF  - Applied Magnetic Resonance
N2  - In this study, a recently proposed NMR standardization approach by 2H integral of deuterated solvent for quantitative multicomponent analysis of complex mixtures is presented. As a proof of principle, the existing NMR routine for the analysis of Aloe vera products was modified. Instead of using absolute integrals of targeted compounds and internal standard (nicotinamide) from 1H-NMR spectra, quantification was performed based on the ratio of a particular 1H-NMR compound integral and 2H-NMR signal of deuterated solvent D2O. Validation characteristics (linearity, repeatability, accuracy) were evaluated and the results showed that the method has the same precision as internal standardization in case of multicomponent screening. Moreover, a dehydration process by freeze drying is not necessary for the new routine. Now, our NMR profiling of A. vera products needs only limited sample preparation and data processing. The new standardization methodology provides an appealing alternative for multicomponent NMR screening. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and is recommended in different application areas (purity determination, forensics, pharmaceutical analysis, etc.).
Y1  - 2021
U6  - http://dx.doi.org/10.1007/s00723-021-01393-4
SN  - 1613-7507
VL  - 52
IS  - 11
SP  - 1591
EP  - 1600
PB  - Springer
CY  - Cham
ER  - 
TY  - CHAP
A1  - Frotscher, Ralf
A1  - Goßmann, Matthias
A1  - Raatschen, Hans-Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Staat, Manfred
T1  - Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM
T2  - Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45)
N2  - We present an electromechanically coupled Finite Element model for cardiac tissue. It bases on the mechanical model for cardiac tissue of Hunter et al. that we couple to the McAllister-Noble-Tsien electrophysiological model of purkinje fibre cells. The corresponding system of ordinary differential equations is implemented on the level of the constitutive equations in a geometrically and physically nonlinear version of the so-called edge-based smoothed FEM for plates. Mechanical material parameters are determined from our own pressure-deflection experimental setup. The main purpose of the model is to further examine the experimental results not only on mechanical but also on electrophysiological level down to ion channel gates. Moreover, we present first drug treatment simulations and validate the model with respect to the experiments.
Y1  - 2015
SN  - 978-3-319-02534-6 ; 978-3-319-02535-3
SP  - 187
EP  - 212
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Küter-Luks, Birgit
A1  - Kern, Heidrun
A1  - Streu, Sandra
A1  - Plum, Leona
A1  - Maurer, Jan
A1  - Kühn, Ralf
A1  - Brüning, Jens C.
A1  - Schwenk, Frieder
T1  - Single copy shRNA configuration for ubiquitous gene knockdown in mice
JF  - Nucleic Acids Research
Y1  - 2005
U6  - http://dx.doi.org/10.1093/nar/gni065
SN  - 1362-4962
VL  - 33
IS  - 7
SP  - e67
ER  - 
TY  - JOUR
A1  - Feng, Yong-Qing
A1  - Seibler, Jost
A1  - Alami, Raouf
A1  - Eisen, Andrew
A1  - Westerman, Karen A.
A1  - Leboulch, Philippe
A1  - Fiering, Steven
A1  - Bouhassira, Eric E.
T1  - Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange
JF  - Journal of Molecular Biology
Y1  - 1999
U6  - http://dx.doi.org/10.1006/jmbi.1999.3113
SN  - 0022-2836
VL  - 292
IS  - 4
SP  - 779
EP  - 785
ER  - 
TY  - JOUR
A1  - Rachinger, Michael
A1  - Bauch, Melanie
A1  - Strittmatter, Axel
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Maurer, Karl-Heinz
A1  - Daniel, Rolf
A1  - Liebl, Wolfgang
A1  - Liesegang, Heiko
A1  - Ehrenreich, Armin
T1  - Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis
JF  - Journal of biotechnology
Y1  - 2013
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - Vol. 164
IS  - Iss. 4
SP  - 365
EP  - 369
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Raupp, Sebastian M.
A1  - Schmitt, Marcel
A1  - Walz, Anna-Lena
A1  - Diehm, Ralf
A1  - Hummel, Helga
A1  - Scharfer, Philip
A1  - Schabel, Wilhelm
T1  - Slot die stripe coating of low viscous fluids
JF  - Journal of Coatings Technology and Research
N2  - Slot die coating is applied to deposit thin and homogenous films in roll-to-roll and sheet-to-sheet applications. The critical step in operation is to choose suitable process parameters within the process window. In this work, we investigate an upper limit for stripe coatings. This maximum film thickness is characterized by stripe merging which needs to be avoided in a stable process. It is shown that the upper limit reduces the process window for stripe coatings to a major extent. As a result, stripe coatings at large coating gaps and low viscosities are only possible for relatively thick films. Explaining the upper limit, a theory of balancing the side pressure in the gap region in the cross-web direction has been developed.
Y1  - 2018
U6  - http://dx.doi.org/10.1007/s11998-017-0039-y
SN  - 1935-3804
VL  - 15
IS  - 5
SP  - 899
EP  - 911
PB  - Springer
ER  - 
TY  - BOOK
A1  - Rath, Walter
A1  - Dzierzynski, Elmar
T1  - Solvent-free contact adhesive = Lösungsmittelfreier Kontaktklebstoff / Rath, Walter ; Dzierzynski, Elmar [Erfinder]
Y1  - 1995
N1  - EP0436347 06.04.1995 Veröffentlichungstag im Patentblatt ; Volltext recherchierbar über:
PB  - Europäisches Patentamt
CY  - München
ER  - 
TY  - JOUR
A1  - Elbers, Gereon
A1  - Buck, Manfred
T1  - Soot Concentration in Ambient Air / M. Buck, G. Elbers
JF  - Erdöl & Kohle, Erdgas, Petrochemie : EKEP. 45 (1992)
Y1  - 1992
SN  - 0014-0058
SP  - 219
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kolz, J.
A1  - Goga, N.
A1  - Casanova, F.
T1  - Spatial localization with single-sided NMR sensors / Kolz, J. ; Goga, N. ; Casanova, F. ; Mang, T. ; Blümich, B.
JF  - Applied Magnetic Resonance. 32 (2007), H. 1-2
Y1  - 2007
SN  - 0937-9347
SP  - 171
EP  - 184
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Scott, Richard
A1  - Näser, Ulrike
A1  - Friedrich, Peter
T1  - Stereochemistry of hydrogen removal from the 'unactivated' C-3 position of 4-hydroxybutyryl-CoA catalysed by 4-hydroxybutyryl-CoA dehydratase / Scott, R. ; Näser, U. ; Friedrich, P. ; Selmer, T. ; Buckel, W. ; Golding, BT.
JF  - Chemical Communications : ChemCom (2004)
Y1  - 2004
SN  - 1364-548X
SP  - 1210
EP  - 1211
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Darley, Dan J.
A1  - Clegg, William
A1  - Harrington, Ross W.
T1  - Stereocontrolled Synthesis of (2R,3S)-2-Methylisocitrate, a Central Intermediate in the Methylcitrate Cycle / Darley, Dan J. ; Selmer, Thorsten ; Clegg, William ; Harrington, Ross W. ; Buckel, Wolfgang ; Golding, Bernardt
JF  - Helvetica chimica acta. 86 (2003), H. 12
Y1  - 2003
SN  - 1522-2675
SP  - 3991
EP  - 3999
ER  - 
TY  - JOUR
A1  - Mang, Thomas
A1  - Kricheldorf, Hans R.
T1  - Stereospecificity of peptide synthesis by means of phosphorus derivatives: a model of peptide synthesis in molecular evolution / Kricheldorf, Hans R. ; Mang, Thomas
JF  - International Journal of Biological Macromolecules. 5 (1983), H. 5
Y1  - 1983
SN  - 0141-8130
SP  - 258
EP  - 266
ER  - 
TY  - JOUR
A1  - Schroeter, Rebecca
A1  - Hoffmann, Tamara
A1  - Voigt, Birgit
A1  - Meyer, Hanna
A1  - Bleisteiner, Monika
A1  - Muntel, Jan
A1  - Jürgen, Britta
A1  - Albrecht, Dirk
A1  - Becher, Dörte
A1  - Lalk, Michael
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Putzer, Harald
A1  - Hecker, Michael
A1  - Schweder, Thomas
A1  - Bremer, Erhard
T1  - Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges
JF  - PLoS ONE
N2  - The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress.
Y1  - 2014
U6  - http://dx.doi.org/10.1371/journal.pone.0080956
SN  - 1932-6203
VL  - 8
IS  - 11
PB  - PLOS
CY  - San Francisco
ER  - 
TY  - JOUR
A1  - Martins, Berta M.
A1  - Blaser, Martin
A1  - Feliks, Mikolaj
A1  - Ullmann, Matthias G.
A1  - Buckel, Wolfgang
A1  - Selmer, Thorsten
T1  - Structural basis for a Kolbe-type decarboxylation catalyzed by a glycyl radical enzyme
JF  - Journal of the American Chemical Society
Y1  - 2011
N1  - Just accepted manuscript
SP  - 1
EP  - 33
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Kotter, Michael
A1  - Lintz, Hans-Günther
A1  - Riekert, Lothar
A1  - Trimm, D. L.
T1  - Structure and selectivity changes in vanadia-titania catalysts used to promote the reduction of nitric oxide with ammonia
JF  - Applied catalysis. 52 (1989), H. 1
Y1  - 1989
SN  - 0166-9834
N1  - ISSN der E-Ausg.: 0166-9834
SP  - 225
EP  - 235
ER  - 
TY  - JOUR
A1  - Merschenz-Quack, Angelika
A1  - Mootz, D.
T1  - Structure of 5-sulfosalicylic acid pentahydrate
JF  - Acta crystallographica / Section C, Crystal structure communications. C 46 (1990), H. 8
Y1  - 1990
SN  - 0108-2701
SP  - 1478
EP  - 1480
ER  - 
TY  - JOUR
A1  - Eckert, Alexander
A1  - Abbasi, Mozhdeh
A1  - Mang, Thomas
A1  - Saalwächter, Kay
A1  - Walther, Andreas
T1  - Structure, Mechanical Properties, and Dynamics of Polyethylenoxide/Nanoclay Nacre-Mimetic Nanocomposites
JF  - Macromolecules
N2  - Nacre-mimetic nanocomposites based on high fractions of synthetic high-aspect-ratio nanoclays in combination with polymers are continuously pushing boundaries for advanced material properties, such as high barrier against oxygen, extraordinary mechanical behavior, fire shielding, and glass-like transparency. Additionally, they provide interesting model systems to study polymers under nanoconfinement due to the well-defined layered nanocomposite arrangement. Although the general behavior in terms of forming such layered nanocomposite materials using evaporative self-assembly and controlling the nanoclay gallery spacing by the nanoclay/polymer ratio is understood, some combinations of polymer matrices and nanoclay reinforcement do not comply with the established models. Here, we demonstrate a thorough characterization and analysis of such an unusual polymer/nanoclay pair that falls outside of the general behavior. Poly(ethylene oxide) (PEO) and sodium fluorohectorite form nacre-mimetic, lamellar nanocomposites that are completely transparent and show high mechanical stiffness and high gas barrier, but there is only limited expansion of the nanoclay gallery spacing when adding increasing amounts of polymer. This behavior is maintained for molecular weights of PEO varied over four orders of magnitude and can be traced back to depletion forces. By careful investigation via X-ray diffraction and proton low-resolution solid-state NMR, we are able to quantify the amount of mobile and immobilized polymer species in between the nanoclay galleries and around proposed tactoid stacks embedded in a PEO matrix. We further elucidate the unusual confined polymer dynamics, indicating a relevant role of specific surface interactions.
Y1  - 2020
U6  - http://dx.doi.org/10.1021/acs.macromol.9b01931
SN  - 1520-5835
VL  - 53
IS  - 5
SP  - 1716
EP  - 1725
PB  - ACS Publications
CY  - Washington, DC
ER  - 
TY  - JOUR
A1  - Merschenz-Quack, Angelika
A1  - Mootz, D.
T1  - Structures of sulfuryl halides: SO2F2, SO2CIF and SO2Cl2 / Mootz, D. ; Merschenz-Quack, A.
JF  - Acta crystallographica / Section C, Crystal structure communications. C 44 (1988), H. 5
Y1  - 1988
SN  - 0108-2701
SP  - 924
EP  - 925
ER  - 
TY  - JOUR
A1  - Merschenz-Quack, Angelika
A1  - Mootz, D.
T1  - Structures of thionyl halides: SOCl2 and SOBr2 / Mootz, D. ; Merschenz-Quack, A.
JF  - Acta crystallographica / Section C, Crystal structure communications. C 44 (1988), H. 5
Y1  - 1988
SN  - 0108-2701
SP  - 926
EP  - 927
ER  - 
TY  - JOUR
A1  - Nokihara, Kiyoshi
A1  - Berndt, Heinz
T1  - Studies on sulfur-containing peptides : tert-butyloxycarbonylsulfenyl and benzyloxycarbonylsulfenyl derivatives as protecting groups for cysteine
JF  - The journal of organic chemistry
Y1  - 1978
U6  - http://dx.doi.org/10.1021/jo00419a046
SN  - 0022-3263
VL  - 43
IS  - 25
SP  - 4893
EP  - 4895
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - PAT
A1  - Bessler, Cornelius
A1  - Maurer, Karl-Heinz
A1  - Merkel, Marion
A1  - Siegert, Petra
A1  - Wieland, Susanne
T1  - Subtilisin from Bacillus Pumilus and detergent and cleaning agents containing said novel subtilisin [US Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2009
SP  - 1
EP  - 39
PB  - USPTO; WIPO
CY  - Washington; Genf
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Andrei, Paula I.
A1  - Pierik, Antonio J.
A1  - Zauner, Stefan
T1  - Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase. A small subunit, HpdC, is essential for catalytic activity / Andrei, PI. ;  Pierik,  AJ. ; Zauner , S. ; Andrei-Selmer, LC.  ; Selmer, T.
JF  - European Journal of Biochemistry. 271 (2004), H. 11
Y1  - 2004
SN  - 0014-2956
SP  - 2225
EP  - 2230
ER  - 
TY  - JOUR
A1  - Selmer, Thorsten
A1  - Recksiek, Michael
A1  - Dierks, Thomas
A1  - Schmidt, Bernhard
T1  - Sulfatases, Trapping of the Sulfated Enzyme Intermediate by Substituting the Active Site Formylglycine / Recksiek, Michael ; Selmer, Thorsten ; Dierks, Thomas ; Schmidt, Bernhard ; Figura, Kurt von
JF  - Journal of Biological Chemistry. 273 (1998), H. 11
Y1  - 1998
SN  - 1083-351X
SP  - 6096
EP  - 6103
ER  - 
TY  - JOUR
A1  - Jeromin, Günter Erich
T1  - Superabsorbed alcohol dehydrogenase—a new catalyst for asymmetric reductions
JF  - Biotechnology Letters. 31 (2009), H. 11
Y1  - 2009
SN  - 0141-5492
SP  - 1717
EP  - 1721
ER  - 
TY  - JOUR
A1  - Al-Kaidy, Huschyar
A1  - Tippkötter, Nils
T1  - Superparamagnetic hydrophobic particles as shell material for digital microfluidic droplets and proof-of-principle reaction assessments with immobilized laccase
JF  - Engineering in Life Sciences
N2  - In the field of biotechnology and molecular biology, the use of small liquid volumes has significant advantages. In particular, screening and optimization runs with acceptable amounts of expensive and hardly available catalysts, reagents, or biomolecules are feasible with microfluidic technologies. The presented new microfluidic system is based on the inclusion of small liquid volumes by a protective shell of magnetizable microparticles. Hereby, discrete aqueous microreactor drops with volumes of 1–30 μL can be formed on a simple planar surface. A digital movement and manipulation of the microreactor is performed by overlapping magnetic forces. The magnetic forces are generated by an electrical coil matrix positioned below a glass plate. With the new platform technology, several discrete reaction compartments can be moved simultaneously on one surface. Due to the magnetic fields, the reactors can even be merged to initiate reactions by mixing or positioned above surface-immobilized catalysts and then opened by magnetic force. Comparative synthesis routes of the magnetizable shell particles and superhydrophobic glass slides including their performance and stability with the reaction platform are described. The influence of diffusive mass transport during the catalyzed reaction is discussed by evaluation finite element model of the microreactor. Furthermore, a first model dye reaction of the enzyme laccase has been established.
Y1  - 2016
U6  - http://dx.doi.org/10.1002/elsc.201400124
VL  - 16
IS  - 3
SP  - 222
EP  - 230
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Jakob, Felix
A1  - Martinez, Ronny
A1  - Mandawe, John
A1  - Hellmuth, Hendrik
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
T1  - Surface charge engineering of a Bacillus gibsonii subtilisin protease
JF  - Applied microbiology and biotechnology
Y1  - 2013
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 97
IS  - Iss. 15
SP  - 6793
EP  - 6802
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Hafidi, Youssef
A1  - El Hatka, Hicham
A1  - Schmitz, Dominik
A1  - Krauss, Manuel
A1  - Pettrak, Jürgen
A1  - Biel, Markus
A1  - Ittobane, Najim
T1  - Sustainable soil additives for water and micronutrient supply: swelling and chelating properties of polyaspartic acid hydrogels utilizing newly developed crosslinkers
JF  - Gels
N2  - Drought and water shortage are serious problems in many arid and semi-arid regions. This problem is getting worse and even continues in temperate climatic regions due to climate change. To address this problem, the use of biodegradable hydrogels is increasingly important for the application as water-retaining additives in soil. Furthermore, efficient (micro-)nutrient supply can be provided by the use of tailored hydrogels. Biodegradable polyaspartic acid (PASP) hydrogels with different available (1,6-hexamethylene diamine (HMD) and L-lysine (LYS)) and newly developed crosslinkers based on diesters of glycine (GLY) and (di-)ethylene glycol (DEG and EG, respectively) were synthesized and characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) and regarding their swelling properties (kinetic, absorbency under load (AUL)) as well as biodegradability of PASP hydrogel. Copper (II) and zinc (II), respectively, were loaded as micronutrients in two different approaches: in situ with crosslinking and subsequent loading of prepared hydrogels. The results showed successful syntheses of di-glycine-ester-based crosslinkers. Hydrogels with good water-absorbing properties were formed. Moreover, the developed crosslinking agents in combination with the specific reaction conditions resulted in higher water absorbency with increased crosslinker content used in synthesis (10% vs. 20%). The prepared hydrogels are candidates for water-storing soil additives due to the biodegradability of PASP, which is shown in an exemple. The incorporation of Cu(II) and Zn(II) ions can provide these micronutrients for plant growth.
KW  - micronutrients
KW  - swelling properties
KW  - biodegradable polymers
KW  - hydrogels
KW  - superabsorbent polymers
KW  - glycine
KW  - polyaspartic acid
Y1  - 2024
U6  - http://dx.doi.org/10.3390/gels10030170
SN  - 2310-2861
VL  - 10
IS  - 3
SP  - Artikel 170
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Meyer-Stork, L. Sebastian
A1  - Höcker, Hartwig
A1  - Berndt, Heinz
T1  - Syntheses and reactions of urethanes of cellobiose and cellulose-containing uretdione groups
JF  - Journal of applied polymer science
Y1  - 1992
SN  - 1097-4628
VL  - 44
IS  - 6
SP  - 1043
EP  - 1049
ER  - 
TY  - CHAP
A1  - Berndt, Heinz
A1  - Turck, Christoph W.
ED  - Voelter, Wolfgang
T1  - Syntheses of defined peptide derivatives by aminolysis of 3-[Nα-benzyloxycarbonyl peptidyloxy] -2-hydroxy-N-methyl-benzamides at elevated temperatures II. Synthesis of the peptide derivatives Z-Ala-X-Gly-N(Et)2, X=Phe, Leu, Val, Ser (But), Glu (OBut)
T2  - Chemistry of peptides and proteins : proceedings of the Fourth USSR-FRG Symposium, Tübingen, Federal Republic of Germany, June 8 - 12, 1982 / ed. Wolfgang Voelter ... - Vol. 2
Y1  - 1984
SN  - 3-11009-580-7
SP  - 97
EP  - 103
PB  - de Gruyter
CY  - Berlin [u.a.]
ER  - 
TY  - JOUR
A1  - Pellegrini, Paul A.
A1  - Howell, Nicholas R.
A1  - Shepherd, Rachael K.
A1  - Lengkeek, Nigel A.
A1  - Paulßen, Elisabeth
A1  - Katsifis, Andrew G.
A1  - Greguric, Ivan
T1  - Synthesis and Radiolabelling of DOTA-Linked Glutamine Analogues with 67,68Ga as Markers for Increased Glutamine Metabolism in Tumour Cells
JF  - Molecules
Y1  - 2013
U6  - http://dx.doi.org/10.3390/molecules18067160
SN  - 1420-3049
VL  - 18
IS  - 6
SP  - 7160
EP  - 7178
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Immel, Timo
A1  - Grützke, Martin
A1  - Späte, Anne-Katrin
A1  - Groth, Ulrich
A1  - Öhlschläger, Peter
A1  - Huhn, Thomas
T1  - Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy
JF  - Chemical Communications
N2  - Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model.
Y1  - 2012
U6  - http://dx.doi.org/10.1039/C2CC31624B
SN  - 1364-548X
VL  - 48
IS  - 46
SP  - 5790
EP  - 5792
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Nokihara, Kiyoshi
A1  - Berndt, Heinz
T1  - Synthesis of hapten–polypeptide conjugates as antigen models for the N-terminal region of the α-2-chain of rabbit skin collagen
JF  - Journal of the Royal Society of Chemistry: Perkin Transactions 1
N2  - Synthesis of derivatives of the peptide sequence L-pyroglutamyl-L-phenylalanyl-L-aspartyl-glycyl-L-lysyl-glycyl-glycyl-glycine as the antigenic determinant representing the N-terminal non-helical region of the α-2-chain of rabbit skin collagen, and conjugation to two different polypeptide carriers, are described.
Y1  - 1978
U6  - http://dx.doi.org/10.1039/P19780000260
SN  - 1364-5463
SN  - 0300-922X
SN  - 1470-4358
VL  - 1978
IS  - 3
SP  - 260
EP  - 263
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Heinze, Daniel
A1  - Mang, Thomas
A1  - Peter, Karin
A1  - Möller, Martin
A1  - Weichold, Oliver
T1  - Synthesis of low molecular weight poly(vinyl acetate) and its application as plasticizer
JF  - Journal of applied polymer science
N2  - Poly(vinyl acetate), PVAc, with a degree of polymerization Xn = 10 was prepared by chain-transfer radical polymerization using carbon tetrachloride and used as oligomeric plasticizer for commercial PVAc. However, the chlorinated chain ends cause a low thermal stability requiring mild Cl/H substitution. The product exhibits high thermal stability and excellent melt-compounding properties. Blends of oligomeric and commercial PVAc show single glass transition temperatures which decrease with higher oligomer content and exhibit small negative deviations from Fox' linear additivity rule. This indicates plasticization and miscibility being mainly due to entropic effects. Injection-moulded thick specimens show ductile behaviour at oligomer contents >10 wt %, while sheets with a thickness of 0.2–0.5 mm appear flexible already at 7.5 wt %. The oxygen permeability coefficients are an order of magnitude lower than those of low-density polyethylene. Due to the sum of their properties, the plasticized sheets present a promising alternative in the preparation of barrier materials.
Y1  - 2014
U6  - http://dx.doi.org/10.1002/app.40226
SN  - 1097-4628 (E-Journal); 0021-8995 (Print)
VL  - 131
IS  - 9
SP  - Article No. 40226
PB  - Wiley
CY  - New York
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Molinnus, Denise
A1  - Jablonski, Melanie
A1  - Kipp, Carina Ronja
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Wagner, Torsten
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
A1  - Siegert, Petra
T1  - Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase
JF  - RSC Advances
N2  - α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated.
Y1  - 2020
U6  - http://dx.doi.org/10.1039/D0RA02066D
SN  - 2046-2069
VL  - 10
SP  - 12206
EP  - 12216
PB  - Royal Society of Chemistry (RSC)
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Muschallik, Lukas
A1  - Kipp, Carina Ronja
A1  - Recker, Inga
A1  - Bongaerts, Johannes
A1  - Pohl, Martina
A1  - Gelissen, Melanie
A1  - Schöning, Michael Josef
A1  - Selmer, Thorsten
A1  - Siegert, Petra
T1  - Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase
JF  - Journal of Biotechnology
N2  - The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.
Y1  - 2020
SN  - 2590-1559
U6  - http://dx.doi.org/10.1016/j.jbiotec.2020.09.016
VL  - 202
IS  - Vol. 324
SP  - 61
EP  - 70
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Paulßen, Elisabeth
A1  - Alberto, Roger
A1  - Abram, Ulrich
T1  - Synthesis, Characterization, and Structures of R3EOTcO3 Complexes (E = C, Si, Ge, Sn, Pb) and Related Compounds
JF  - Inorganic Chemistry
N2  - AgTcO4 reacts with R3ECl compounds (E = C, Si, Ge, Sn, Pb; R = Me, iPr, tBu, Ph), tBu2SnCl2, or PhMgCl under formation of novel trioxotechnetium(VII) derivatives. The carbon and silicon derivatives readily undergo decomposition, which was proven by 99Tc NMR spectroscopy and the isolation of decomposition products such as [TcOCl3(THF)(OH2)]. Compounds [Ph3GeOTcO3], [(THF)Ph3SnOTcO3], [(O3TcO)SntBu2(OH)]2, and [(THF)4Mg(OTcO3)2] are more stable and were isolated in crystalline form and characterized by X-ray diffraction.
Y1  - 2010
U6  - http://dx.doi.org/10.1021/ic1001094
SN  - 1520-510X
VL  - 49
IS  - 7
SP  - 3525
EP  - 3530
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Öhlschläger, Peter
A1  - Michel, Nico
A1  - Osen, Wolfram
A1  - Freyschmidt, Eva-Jasmin
T1  - T cell response to human papillomavirus 16 E7 in mice: comparison of Cr release assay, intracellular IFN-gamma production, ELISPOT and tetramer staining / Michel, Nico ; Öhlschläger, Peter ; Osen, Wolfram ; Freyschmidt, Eva-Jasmin ; Gutöhrlein, Heidrun ;
JF  - Intervirology. 45 (2002)
Y1  - 2002
SN  - 1423-0100
SP  - 290
EP  - 299
ER  - 
TY  - JOUR
A1  - Delaittre, Guillaume
T1  - Telechelic Poly(2-Oxazoline)s
JF  - European Polymer Journal
Y1  - 2019
U6  - http://dx.doi.org/10.1016/j.eurpolymj.2019.109281
SN  - 0014-3057
IS  - In Press, Journal Pre-proof, 109281
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Jeromin, Günter Erich
T1  - Tetrabenzo[a,c,h,j]phenoxazine-18-yl a new stable neutral radical
JF  - Tetrahedron Letters  . 42 (2001), H. 10
Y1  - 2001
SN  - 0040-4039
N1  - ISSN der E-Ausg.: 1359-8562
SP  - 1863
EP  - 1865
ER  -