TY  - PAT
A1  - Bessler, Cornelius
A1  - Maurer, Karl-Heinz
A1  - Merkel, Marion
A1  - Siegert, Petra
A1  - Wieland, Susanne
T1  - Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin [Offenlegungsschrift]
T1  - Subtilisin from Bacillus pumilus and washing and cleaning agents containing said novel subtilisin [Europäische Patentanmeldung / Internationale Patentanmeldung / Kanadische Patentanmeldung]
Y1  - 2007
SP  - 1
EP  - 47
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO / CIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - JOUR
A1  - Martinez, Ronny
A1  - Jakob, Felix
A1  - Tu, Ran
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
T1  - Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution
JF  - Biotechnology and bioengineering
Y1  - 2013
SN  - 1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print)
VL  - Vol. 110
IS  - Iss. 3
SP  - 711
EP  - 720
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Jakob, Felix
A1  - Martinez, Ronny
A1  - Mandawe, John
A1  - Hellmuth, Hendrik
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
T1  - Surface charge engineering of a Bacillus gibsonii subtilisin protease
JF  - Applied microbiology and biotechnology
Y1  - 2013
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 97
IS  - Iss. 15
SP  - 6793
EP  - 6802
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Niehaus, F.
A1  - Gabor, E.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Eck, J.
T1  - Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases
JF  - Microbial biotechnology
Y1  - 2011
SN  - 1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)
VL  - Vol. 4
IS  - Iss. 6
SP  - 767
EP  - 776
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Heumann, S.
A1  - Karl, W.
A1  - Gerlach, J.
A1  - Leber, R.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Siegert, Petra
A1  - Lange, J.
A1  - Maurer, Karl-Heinz
A1  - Berg, G.
A1  - Guebitz, G. M.
A1  - Schwab, H.
T1  - Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli
JF  - Journal of biotechnology
N2  - A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3.

From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.
KW  - Alginate beads
KW  - Stenotrophomonas maltophilia
KW  - Detergent protease
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiotec.2011.09.025
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 157
IS  - 1
SP  - 140
EP  - 147
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Ribitsch, D.
A1  - Karl, W.
A1  - Birner-Gruenberger, R.
A1  - Gruber, K.
A1  - Eiteljoerg, I.
A1  - Remler, P.
A1  - Wieland, S.
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schwab, H.
T1  - C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli
JF  - Journal of biotechnology
N2  - Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477.

This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.
Y1  - 2010
U6  - http://dx.doi.org/10.1016/j.jbiotec.2010.09.947
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
VL  - 150
IS  - 3
SP  - 408
EP  - 416
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Wendorff, Marion
A1  - Eggert, Thorsten
A1  - Pohl, Martina
A1  - Dresen, Carola
A1  - Müller, Michael
A1  - Jaeger, Karl-Erich
A1  - Sprenger, Georg A.
A1  - Schürmann, Melanie
A1  - Schürmann, Martin
A1  - Johnen, Sandra
A1  - Sprenger, Gerda
A1  - Sahm, Hermann
A1  - Inoue, Tomoyuki
A1  - Schörken, Ulrich
A1  - Breittaupt, Holger
A1  - Frölich, Bettina
A1  - Heim, Petra
A1  - Iding, Hans
A1  - Juchem, Bettina
A1  - Siegert, Petra
A1  - Kula, Maria-Regina
A1  - Weckbecker, Andrea
A1  - Hummel, Werner
A1  - Fessner, Wolf-Dieter
A1  - Elling, Lothar
A1  - Wolberg, Michael
A1  - Bode, Silke
A1  - Feldmann, Ralf
A1  - Geilenkirchen, Petra
A1  - Schubert, Thomas
A1  - Walter, Lydia
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Kolter-Jung, Doris
A1  - Nitsche, Adam
A1  - Dünkelmann, Pascal
A1  - Cosp, Annabel
A1  - Lingen, Bettina
T1  - Catalytic asymmetric synthesis : section 2.2
T2  - Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ...
Y1  - 2007
SN  - 978-3-527-31473-7
SP  - 298
EP  - 413
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Siegert, Petra
A1  - McLeish, Michael J.
A1  - Baumann, Martin
A1  - Iding, Hans
A1  - Kneen, Malea M.
A1  - Kenyon, George L.
A1  - Pohl, Martina
T1  - Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida
JF  - Protein engineering, design, and selection : peds
Y1  - 2005
SN  - 1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print)
VL  - Vol. 18
IS  - Iss. 7
SP  - 345
EP  - 357
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Kluin, Cornelia
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas [Offenlegungsschrift]
T1  - Detergents and cleaners comprising proteases from xanthomonas [Internationale Patentanmeldung]
Y1  - 2008
SP  - 1
EP  - 15
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  - 
TY  - THES
A1  - Huber, Eugen
T1  - Selektive Reduktion von bifunktionellen aromatischen Carbonylverbindungen
Y1  - 2014
PB  - FH Aachen
CY  - Aachen
ER  - 
TY  - CHAP
A1  - Siegert, Petra
A1  - Pohl, Martina
A1  - Kneen, Malea M.
A1  - Pogozheva, Irina D.
A1  - Kenyon, George L.
A1  - McLeish, Michael J.
T1  - Exploring the substrate specificity of benzoylformate decarboxylase, pyruvate decarboxylase, and benzaldehyde lyase
T2  - Thiamine : catalytic mechanisms in normal and disease states / ed. by Frank Jordan ...
Y1  - 2004
SN  - 0-8247-4062-9
SP  - 275
EP  - 290
PB  - Dekker
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Dünkelmann, Pascal
A1  - Kolter-Jung, Doris
A1  - Nitsche, Adam
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Lingen, Bettina
A1  - Baumann, Martin
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation
JF  - Journal of the American Chemical Society
Y1  - 2002
SN  - 1520-5126 (E-Journal); 0002-7863 (Print)
VL  - Vol. 124
SP  - 12084
EP  - 12085
ER  - 
TY  - JOUR
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation
JF  - Cheminform
Y1  - 2001
SN  - 1522-2667 (E-Journal); 0931-7597 (Print)
VL  - Vol. 32
IS  - Iss. 4
SP  - Publ. online
ER  - 
TY  - JOUR
A1  - Dünnwald, Thomas
A1  - Demir, Ayhan S.
A1  - Siegert, Petra
A1  - Pohl, Martina
A1  - Müller, Michael
T1  - Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C−C Bond Formation
JF  - European journal of organic chemistry
Y1  - 2000
SN  - 0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print)
VL  - Vol. 2000
IS  - Iss. 11
SP  - 2161
EP  - 2170
ER  - 
TY  - CHAP
A1  - Siegert, Petra
A1  - Iding, Hans
A1  - Baumann, Martin
A1  - McLeish, Michael J.
A1  - Kenyon, George L.
A1  - Pohl, Martina
T1  - Broadening of the substrate spectra of two ThDP-dependent decarboxylases using site-directed-mutagenesis
T2  - Proceedings of the 4th International Congress on Biochemical Engineering : 17 and 18 February 2000, Stuttgart
Y1  - 2000
SN  - 3-8167-5570-4
SP  - 38
EP  - 42
ER  - 
TY  - JOUR
A1  - Iding, Hans
A1  - Dünnwald, Thomas
A1  - Greiner, Lasse
A1  - Liese, Andreas
A1  - Müller, Michael
A1  - Siegert, Petra
A1  - Grötzinger, Joachim
A1  - Demir, Ayhan S.
A1  - Pohl, Martina
T1  - Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones
JF  - Chemistry - a European journal
Y1  - 2000
SN  - 1521-3765 (E-Journal); 0947-6539 (Print)
VL  - Vol. 6
IS  - Iss. 8
SP  - 1483
EP  - 1495
ER  - 
TY  - JOUR
A1  - Iding, Hans
A1  - Siegert, Petra
A1  - Mesch, K.
A1  - Pohl, Martina
T1  - Application of α-keto acid decarboxylases in biotransformations
JF  - Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology
Y1  - 1998
SN  - 1879-2588 (E-Journal); 0167-4838 (Print)
VL  - Vol. 1385
IS  - Iss. 2
SP  - 307
EP  - 322
ER  - 
TY  - JOUR
A1  - Pohl, Martina
A1  - Siegert, Petra
A1  - Mesch, K.
A1  - Bruhn, H.
A1  - Grötzinger, Joachim
T1  - Active site mutants of pyruvate decarboxylase from Zymomonas mobilis : a site-directed mutagenesis study of L112, I472, I476, E473 and N482
JF  - European journal of biochemistry
Y1  - 1998
SN  - 1432-1033 (E-Journal); 1742-4658 (E-Journal); 0014-2956 (Print); 1742-464X (Print)
VL  - Vol. 257
IS  - Iss. 3
SP  - 538
EP  - 546
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Evers, Stefan
A1  - Marion, Merkel
A1  - Mussmann, Nina
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Schwaneberg, Ulrich
A1  - Martinez, Ronny
A1  - Jakob, Felix
T1  - Verfahren zur Anpassung eines hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente [Offenlegungsschrift]
T1  - Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2013
SP  - 1
EP  - 27
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Siegert, Petra
A1  - Weber, Thomas
A1  - Tondera, Susanne
A1  - Hellmuth, Hendrik
T1  - Flüssige Tensidzubereitung enthaltend Lipase und Phosphonat [Offenlegungsschrift]
T1  - Liquid surfactant preparation containing lipase and phosphonate [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 22
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierenden Komponente, die eine Phenylalkyldicarbonsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (using a component that stabilizes the hydrolytic enzyme and includes a phenylalkyldicarboxylic acid) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 15
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (durch den Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine mehrfach substituierte Benzolcarbonsäure umfasst, die an mindestens zwei Kohlenstoffatomen des Benzolrestes eine Carboxylgruppe aufweist) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising a multiply substituted benzyl carboxylic acid comprising a carboxyl group on at least two carbon atoms of the benzyl radical is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Phthaloylglutaminsäure und/oder eine Phthaloylasparaginsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising a phthaloyl glutamine acid and/or a phthaloyl asparagine acid is used for stablizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Aminophthalsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising an aminophthalic acid is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die eine Oligoamino-biphenyl-oligocarbonsäure umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid tenside preparation comprising enzymes (a component comprising an oligoamino-biphenyl-oligocarboxylic acid is used for stabilizing the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 16
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Hellmuth, Hendrik
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Stabilisierte flüssige enzymhaltige Tensidzubereitung (Einsatz einer das hydrolytische Enzym stabilisierende Komponente, die ein Monosaccharidglycerat umfasst) [Offenlegungsschrift]
T1  - Stabilized liquid enzyme-containing surfactant preparation (using a component which comprises a monosaccharide glycerate and which stabilizes the hydrolytic enzyme) [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 17
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Merkel, Marion
A1  - Kluin, Cornelia
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Wieland, Susanne
A1  - Hellmuth, Hendrik
T1  - Neue Proteasen und diese enthaltende Mittel [Offenlegungsschrift]
T1  - Novel proteases and compositions comprising them [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2011
SP  - 1
EP  - 21
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Schwaneberg, Ulrich
A1  - Martinez Moya, Ronny
A1  - Merkel, Marion
A1  - Spitz, Astrid
A1  - Wieland, Susanne
A1  - Hellmuth, Hendrik
A1  - Maurer, Karl-Heinz
T1  - Leistungsverbesserte Proteasevariante [Offenlegungsschrift]
T1  - Performance-enhanced protease variant [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2012
SP  - 1
EP  - 29
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Wieland, Susanne
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
A1  - O'Connell, Timothy
A1  - Prüser, Inken
A1  - Schiedel, Marc-Steffen
A1  - Eiting, Thomas
A1  - Sendor-Müller, Dorota
A1  - Bastigkeit, Thorsten
A1  - Benda, Konstantin
A1  - Müller, Sven
T1  - Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen [Offenlegungsschrift]
T1  - Storage-stable liquid washing or cleaning agent containing proteases [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2011
SP  - 1
EP  - 25
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Mussmann, Nina
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Novel proteases and means containing said proteases [Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 30
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Baumstark, Rebecca
A1  - Kluin, Cornelia
A1  - O'Connell, Timothy
A1  - Maurer, Karl-Heinz
A1  - Hellmuth, Hendrik
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
Y1  - 2010
SP  - 1
EP  - 30
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
T1  - Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]
T1  - Novel proteases and compositions comprising these proteases [Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 31
PB  - Deutsches Patentamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Spitz, Astrid
A1  - Maurer, Karl-Heinz
T1  - Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus pumilus [Offenlegungsschrift]
T1  - Detergents and cleaning agents containing proteases from bacillus pumilus [Europäische Patentschrift / Internationale Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 20
PB  - Deutsches Patentamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - O'Connell, Timothy
A1  - Siegert, Petra
A1  - Evers, Stefan
A1  - Bongaerts, Johannes
A1  - Weber, Thomas
A1  - Maurer, Karl-Heinz
A1  - Bessler, Cornelius
T1  - Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft [Offenlegungsschrift]
T1  - Method from improving the cleaning action of a detergent of cleaning agent [US Patentanmeldung]
Y1  - 2010
SP  - 1
EP  - 34
PB  - Deutsches Patentamt
CY  - München
ER  - 
TY  - PAT
A1  - Berg, Gabriele
A1  - Bessler, Cornelius
A1  - Gerlach, Jochen
A1  - Gübitz, Georg
A1  - Heumann, Sonja
A1  - Karl, Wolfgang
A1  - Maurer, Karl-Heinz
A1  - Remler, Peter
A1  - Ribitsch, Doris
A1  - Schwab, Helmut
A1  - Siegert, Petra
A1  - Wieland, Susanne
T1  - Mittel enthaltend Proteasen aus Stenotrophomonas maltophilia [Offenlegungsschrift]
T1  - Agents containing proteases from Stenotrophomonas maltophilia [Europäische Patentanmeldung / US Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2009
SP  - 1
EP  - 47
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / USPTO / WIPO
CY  - München / Den Hague / Washington / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Wieland, Susanne
A1  - Engelskirchen, Julia
A1  - Merkel, Marion
A1  - Maurer, Karl-Heinz
A1  - Bessler, Cornelius
T1  - Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease [Offenlegungsschrift]
T1  - Novel alkaline protease from Bacillus Gibsonii and washing and cleaning agents containing said novel alkaline protease [Europäische Patentanmeldung / US Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2008
SP  - 1
EP  - 51
PB  - Deutsches Patentamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Merkel, Marion
A1  - Weber, Angrit
A1  - Siegert, Petra
A1  - Wieland, Susanne
A1  - Maurer, Karl-Heinz
A1  - Bessler, Cornelius
T1  - Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease [Offenlegungsschrift]
T1  - Novel alkaline protease from Bacillus Gibsonii and washing and cleaning agents containing said novel alkaline protease [Internationale Patentanmeldung]
Y1  - 2007
SP  - 1
EP  - 46
PB  - Deutsches Patent- und Markenamt / WIPO
CY  - München / Genf
ER  - 
TY  - PAT
A1  - O'Connell, Timothy
A1  - Hoven, Nina
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
T1  - Amadoriasen in Wasch- und Reinigungsmitteln [Offenlegungsschrift]
T1  - Amadoriases in washing and cleaning products [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2007
SP  - 1
EP  - 45
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Schulze zur Wiesche, Erik
A1  - Otto, Ralf
A1  - Kleen, Astrid
A1  - Hollenberg, Detlef
A1  - Sättler, Andrea
A1  - Siegert, Petra
A1  - Boßmann, Britta
T1  - Verfahren zur dauerhaften Ausrüstung keratinischer Fasern mit Pflegewirkstoffen durch Carboxylesterhydrolasen [Offenlegungsschrift]
T1  - Method for the permanent deposition of conditioning agents on keratinic fibres using carboxylester hydrolases [Europäische Patentanmeldung]
Y1  - 2005
SP  - 1
EP  - 13
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt
CY  - München / Den Hague
ER  - 
TY  - PAT
A1  - Kluin, Cornelia
A1  - Maurer, Karl-Heinz
A1  - Banowski, Bernhard
A1  - Bessler, Cornelius
A1  - Siegert, Petra
T1  - Verwendung von Siderophoren gegen Geruchskeime [Offenlegungsschrift]
T1  - Use of siderophores for control of odor-producing bacteria, particularly for treating or preventing body odor, act by depriving bacteria of iron
Y1  - 2005
SP  - 1
EP  - 32
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - PAT
A1  - Bankowski, Bernhard
A1  - Hoffmann, Daniele
A1  - Wadle, Armin
A1  - Siegert, Petra
A1  - Sättler, Andrea
A1  - Gerke, Thomas
T1  - Beta-Glucuronidase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - Beta-Glucuronidase inhibitors for use in deodorants and antiperspirants [Europäische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2003
SP  - 1
EP  - 24
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / WIPO
CY  - München / Den Hague / Genf
ER  - 
TY  - PAT
A1  - Siegert, Petra
A1  - Bankowski, Bernhard
T1  - Verwendung von Steroidsulfatase-Inhibitoren zur Verminderung von Haarausfall ; Kosmetische und pharmazeutische Zusammensetzungen enthaltend Steroidsulfatase-Inhibitoren und deren Verwendung zur Verminderung von Haarausfall [Offenlegungsschrift]
T1  - Cosmetic and pharmaceutical compositions comprising steroid sulfatase inhibitors and their use for the reduction of hair loss [Europäische Patentanmeldung]
Y1  - 2003
SP  - 1
EP  - 38
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt
CY  - München / Den Hague
ER  - 
TY  - PAT
A1  - Banowski, Bernhard
A1  - Wadle, Armin
A1  - Siegert, Petra
T1  - Arylsulfatase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - Arylsulfatase inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung]
Y1  - 2004
SP  - 1
EP  - 15
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt
CY  - München / Den Hague
ER  - 
TY  - PAT
A1  - Banowski, Bernhard
A1  - Wadle, Armin
A1  - Siegert, Petra
T1  - Arylsulfatase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - Reducing body odor, e.g. in men, comprises applying deodorant or antiperspirant composition containing arylsulfatase-inhibiting phenoxyphenol derivative to skin
Y1  - 2003
SP  - 1
EP  - 20
PB  - Deutsches Patent- und Markenamt
CY  - München
ER  - 
TY  - PAT
A1  - Banowski, Bernhard
A1  - Hoffmann, Daniele
A1  - Wadle, Armin
A1  - Siegert, Petra
A1  - Sättler, Andrea
A1  - Gerke, Thomas
T1  - Arylsulfatase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - Arylsufatas-inhibitors in deodorants and antiperspirants[Europäische Patentschrift / Österreichische Patentanmeldung / Polnische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2002
SP  - 1
EP  - 22
PB  - Deutsches Patent- und Markenamt / Polish Patent Office / WIPO
CY  - München / Warsaw / Genf
ER  - 
TY  - PAT
A1  - Banowski, Bernhard
A1  - Wadle, Armin
A1  - Siegert, Petra
T1  - Use of hydroxydiphenyl ether derivatives as arylsulfatase-inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung / US Patentanmeldung / Australische Patentanmeldung / Internationale Patentanmeldung]
T1  - Verwendung von Hydroxydiphenylether-Derivaten als Arylsulfatase-Hemmer in Deodorants und Antitranspirantien
Y1  - 2005
SP  - 1
EP  - 40
PB  - Europäisches Patentamt / USPTO / IP Australia / WIPO
CY  - Den Hague / Washington / Canberra/ Genf
ER  - 
TY  - PAT
A1  - Schmitz, Susanne
A1  - Spitz, Astrid
A1  - Merkel, Marion
A1  - Siegert, Petra
T1  - Verfahren zur Bestimmung des enzymatischen Status humaner Haut in vitro [Offenlegungsschrift]
T1  - Method for determining the enzymatic status of human skin in vitro [Europäische Patentanmeldung / Australische Patentanmeldung / Internationale Patentanmeldung]
Y1  - 2004
SP  - 1
EP  - 7
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt / IP Australia / WIPO
CY  - München / Den Hague / Canberra / Genf
ER  - 
TY  - PAT
A1  - Banowski, Bernhard
A1  - Wadle, Armin
A1  - Siegert, Petra
A1  - Sättler, Andrea
T1  - Lipase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - Lipase inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung]
Y1  - 2004
SP  - 1
EP  - 22
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt
CY  - München / Den Hague
ER  - 
TY  - PAT
A1  - Banowski, Bernhard
A1  - Waldmann-Laue, Marianne
A1  - Wadle, Armin
A1  - Siegert, Petra
A1  - Sättler, Andreas
T1  - 5-Lipoxigenase-Inhibitoren in Deodorantien und Antitranspirantien [Offenlegungsschrift]
T1  - 5-Lipooxygenase inhibitors in deodorants and antiperspirants [Europäische Patentanmeldung]
Y1  - 2004
SP  - 1
EP  - 12
PB  - Deutsches Patent- und Markenamt / Europäisches Patentamt
CY  - München / Den Hague
ER  - 
TY  - JOUR
A1  - Ratke, Lorenz
A1  - Milow, Barbara
A1  - Lisinski, Susanne
A1  - Hoepfner, Sandra
T1  - On an effect of fine ceramic particles on the structure of aerogels
JF  - Microgravity science and technology
Y1  - 2014
U6  - http://dx.doi.org/10.1007/s12217-014-9380-2
SN  - 0938-0108 ; 1875-0494
VL  - 26
SP  - 103
EP  - 110
PB  - Springer Nature
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Handtke, Stefan
A1  - Volland, Sonja
A1  - Methling, Karen
A1  - Albrecht, Dirk
A1  - Becher, Dörte
A1  - Nehls, Jenny
A1  - Bongaerts, Johannes
A1  - Maurer, Karl-Heinz
A1  - Lalk, Michael
A1  - Liesegang, Heiko
A1  - Voigt, Birgit
A1  - Daniel, Rolf
A1  - Hecker, Michael
T1  - Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach
JF  - Journal of Biotechnology
N2  - Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC–MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.
Y1  - 2014
U6  - http://dx.doi.org/10.1016/j.jbiotec.2014.08.028
SN  - 1873-4863 (E-Journal); 0168-1656 (Print)
IS  - 192(A)
SP  - 204
EP  - 214
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Nachtrodt, Frederik
A1  - Tietsch, Wolfgang
A1  - Mostacci, Domiziano
A1  - Scherer, Ulrich W.
T1  - Set-up and first operation of a plasma oven for treatment of low level radioactive wastes
JF  - Nuclear technology and radiation protection
Y1  - 2014
U6  - http://dx.doi.org/10.2298/NTRP140SS47N
SN  - 1451-3994
VL  - 29
IS  - Suppl.
SP  - 47
EP  - 51
PB  - VINČA Institute of Nuclear Sciences
CY  - Belgrad
ER  - 
TY  - CHAP
A1  - Frotscher, Ralf
A1  - Goßmann, Matthias
A1  - Raatschen, Hans-Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Staat, Manfred
T1  - Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM
T2  - Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45)
N2  - We present an electromechanically coupled Finite Element model for cardiac tissue. It bases on the mechanical model for cardiac tissue of Hunter et al. that we couple to the McAllister-Noble-Tsien electrophysiological model of purkinje fibre cells. The corresponding system of ordinary differential equations is implemented on the level of the constitutive equations in a geometrically and physically nonlinear version of the so-called edge-based smoothed FEM for plates. Mechanical material parameters are determined from our own pressure-deflection experimental setup. The main purpose of the model is to further examine the experimental results not only on mechanical but also on electrophysiological level down to ion channel gates. Moreover, we present first drug treatment simulations and validate the model with respect to the experiments.
Y1  - 2015
SN  - 978-3-319-02534-6 ; 978-3-319-02535-3
SP  - 187
EP  - 212
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Raue, Markus
A1  - Wambach, M.
A1  - Glöggler, S.
A1  - Grefen, Dana
A1  - Kaufmann, R.
A1  - Abetz, C.
A1  - Georgopanos, P.
A1  - Handge, U. A.
A1  - Mang, Thomas
A1  - Blümich, B.
A1  - Abetz, V.
T1  - Investigation of historical hard rubber ornaments of Charles Goodyear
JF  - Macromolecular chemistry and physics
Y1  - 2014
SN  - 1022-1352
VL  - Vol. 215
IS  - No. 3
SP  - 245
EP  - 254
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Seifarth, Volker
A1  - Goßmann, Matthias
A1  - Grosse, J. O.
A1  - Becker, C.
A1  - Heschel, I.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds
JF  - Urologia Internationalis
Y1  - 2015
U6  - http://dx.doi.org/10.1159/000368419
SN  - 0042-1138
VL  - 2015
IS  - 95
SP  - 106
EP  - 113
PB  - Karger
CY  - Basel
ER  - 
TY  - JOUR
A1  - Kusch, Peter
A1  - Rieser, Claudia
A1  - Knupp, Gerd
A1  - Mang, Thomas
T1  - Characterization of copolymers of methacrylic acid with poly(ethylene glycol) methyl ether methacrylate macromonomers by analytical pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS)
JF  - Journal of Analytical and Applied Pyrolysis
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.jaap.2015.03.003
SN  - 0165-2370
VL  - Vol. 113
SP  - 412
EP  - 418
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Iken, Heiko
A1  - Selmer, Thorsten
A1  - Keusgen, Michael
A1  - Schöning, Michael Josef
T1  - Development of a multi‐parameter sensor chip for the simultaneous detection of organic compounds in biogas processes
JF  - Physica status solidi (a)
N2  - An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431894
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1306
EP  - 1312
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Kirschbaum, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide
JF  - Physica status solidi (a)
N2  - Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201431944
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1368
EP  - 1374
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Raab, Monika
A1  - Kappel, Sven
A1  - Krämer, Andrea
A1  - Sanhaji, Mourad
A1  - Matthess, Yves
A1  - Kurunci-Csacsko, Elisabeth
A1  - Calzada-Wack, Julia
A1  - Rathkolb, Birgit
A1  - Rosman, Jan
A1  - Adler, Thure
A1  - Busch, Dirk H.
A1  - Esposito, Irene
A1  - Fuchs, Helmut
A1  - Gailus-Durner, Valérie
A1  - Klingenspor, Martin
A1  - Wolf, Eckhard
A1  - Sänger, Nicole
A1  - Prinz, Florian
A1  - Hrabe de Angelis, Martin
A1  - Seibler, Jost
A1  - Yuan, Juping
A1  - Bergmann, Martin
A1  - Knecht, Rainald
A1  - Kreft, Bertolt
A1  - Strebhardt, Klaus
T1  - Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells
JF  - Nature Communications
Y1  - 2011
U6  - http://dx.doi.org/10.1038/ncomms1395
SN  - 2041-1723
VL  - 2
IS  - 395
SP  - 1
EP  - 11
PB  - Nature
CY  - London
ER  - 
TY  - JOUR
A1  - Hasegawa, Maki
A1  - Kapelyukh, Yury
A1  - Tahara, Harunobu
A1  - Seibler, Jost
A1  - Rode, Anja
A1  - Krueger, Sylvia
A1  - Lee, Dongtao N.
A1  - Wolf, C. Roland
A1  - Scheer, Nico
T1  - Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line
JF  - Molecular Pharmacology
Y1  - 2011
U6  - http://dx.doi.org/10.1124/mol.111.071845
SN  - 1521-0111
VL  - 80
IS  - 33
SP  - 518
EP  - 528
PB  - ASPET
CY  - Bethesda, Md.
ER  - 
TY  - JOUR
A1  - Jordan, Sabine D.
A1  - Krüger, Markus
A1  - Willmes, Diana M.
A1  - Redemann, Nora
A1  - Wunderlich, F. Thomas
A1  - Brönneke, Hella S.
A1  - Merkwirth, Carsten
A1  - Kashkar, Hamid
A1  - Olkkonen, Vesa M.
A1  - Böttger, Thomas
A1  - Braun, Thomas
A1  - Seibler, Jost
A1  - Brüning, Jens C.
T1  - Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism
JF  - Nature Cell Biology
N2  - The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.
Y1  - 2011
U6  - http://dx.doi.org/10.1038/ncb2211
SN  - 1465-7392
VL  - 13
IS  - 4
SP  - 434
EP  - 446
PB  - Nature
CY  - New York
ER  - 
TY  - CHAP
A1  - Seibler, Jost
A1  - Schwenk, Frieder
T1  - Transgenic RNAi Applications in the Mouse
T2  - Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition
Y1  - 2010
SN  - 978-0-12-384880-2
N1  - Methods in Enzymology : Vol. 477
SP  - 367
EP  - 386
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Glaser, Stefan
A1  - Lubitz, Sandra
A1  - Loveland, Kate L.
A1  - Ohbo, Kazu
A1  - Robb, Lorraine
A1  - Schwenk, Frieder
A1  - Seibler, Jost
A1  - Roellig, Daniela
A1  - Kranz, Andrea
A1  - Anastassiadis, Konstantinos
A1  - Stewart, A. Francis
T1  - The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis
JF  - Epigenetics & Chromatin
Y1  - 2009
U6  - http://dx.doi.org/10.1186/1756-8935-2-5
SN  - 1756-8935
VL  - 2
IS  - 5
SP  - 1
EP  - 16
ER  - 
TY  - JOUR
A1  - Kotnik, Katarina
A1  - Popova, Elena
A1  - Todiras, Mihail
A1  - Mori, Marcelo A.
A1  - Alenina, Natalia
A1  - Seibler, Jost
A1  - Bader, Michael
T1  - Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown
JF  - Plos One
Y1  - 2009
U6  - http://dx.doi.org/10.1371/journal.pone.0005124
VL  - 4
IS  - 4
SP  - e5124
PB  - Plos
CY  - San Francisco, California, US
ER  - 
TY  - JOUR
A1  - Herold, Marco J.
A1  - Brandt, Jens van den
A1  - Seibler, Jost
A1  - Reichardt, Holger M.
T1  - Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats
JF  - Proceedings of the National Academy of Sciences of the United States of America
Y1  - 2008
U6  - http://dx.doi.org/10.1073/pnas.0806213105
SN  - 1091-6490
VL  - 105
IS  - 47
SP  - 18507
EP  - 18512
ER  - 
TY  - JOUR
A1  - Koch, Linda
A1  - Wunderlich, F. Thomas
A1  - Seibler, Jost
A1  - Könner, A. Christine
A1  - Hampel, Brigitte
A1  - Irlenbusch, Sigrid
A1  - Brabant, Georg
A1  - Kahn, C. Ronald
A1  - Schwenk, Frieder
A1  - Brüning, Jens C.
T1  - Central insulin action regulates peripheral glucose and fat metabolism in mice
JF  - The Journal of Clinical Investigation (JCI)
Y1  - 2008
U6  - http://dx.doi.org/10.1172/JCI31073
SN  - 1558-8238
VL  - 118
IS  - 6
SP  - 2132
EP  - 2147
ER  - 
TY  - JOUR
A1  - Christoph, Thomas
A1  - Bahrenberg, Gregor
A1  - Vry, Jean de
A1  - Englberger, Werner
A1  - Erdmann, Volker A.
A1  - Frech, Moritz
A1  - Kögel, Babette
A1  - Röhl, Thomas
A1  - Schiene, Klaus
A1  - Schröder, Wolfgang
A1  - Seibler, Jost
A1  - Kurreck, Jens
T1  - Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice
JF  - Molecular and Cellular Neuroscience
Y1  - 2008
U6  - http://dx.doi.org/10.1016/j.mcn.2007.12.006
SN  - 1044-7431
VL  - 37
IS  - 3
SP  - 579
EP  - 589
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Kleinridders, Andre
A1  - Küter-Luks, Birgit
A1  - Niehaves, Sandra
A1  - Brüning, Jens C.
A1  - Schwenk, Frieder
T1  - Reversible gene knockdown in mice using a tight, inducible shRNA expression system
JF  - Nucleic Acids Research
Y1  - 2007
U6  - http://dx.doi.org/10.1093/nar/gkm122
SN  - 1362-4962
VL  - 35
IS  - 7
SP  - e54
ER  - 
TY  - JOUR
A1  - Scheer, Nico
A1  - Snaith, Mike
A1  - Wolf, C. Roland
A1  - Seibler, Jost
T1  - Generation and utility of genetically humanized mouse models
JF  - Drug Discovery Today
Y1  - 2013
U6  - http://dx.doi.org/10.1016/j.drudis.2013.07.007
SN  - 1359-6446
VL  - Vol 18
IS  - 23-24
SP  - 1200
EP  - 1211
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Bouwman, Peter
A1  - Gulden, Hanneke van der
A1  - Heijden, Ingrid van der
A1  - Drost, Rinske
A1  - Klijn, Christiaan N.
A1  - Prasetyanti, Pramudita
A1  - Pieterse, Mark
A1  - Wientjens, Ellen
A1  - Seibler, Jost
A1  - Hogervorst, Frank B. L.
A1  - Jonkers, Jos
T1  - A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants
JF  - Cancer Discovery
Y1  - 2013
U6  - http://dx.doi.org/10.1158/2159-8290.CD-13-0094
SN  - 2159-8290
IS  - 3
SP  - 1142
EP  - 1152
ER  - 
TY  - JOUR
A1  - Michalak, Ewa Malgorzata
A1  - Nacerddine, Karim
A1  - Pietersen, Alexandra
A1  - Beuger, Vincent
A1  - Pawlitzky, Inka
A1  - Cornelissen-Steijger, Paulien
A1  - Wientjens, Ellen
A1  - Tanger, Ellen
A1  - Seibler, Jost
A1  - Lohuizen, Maarten van
A1  - Jonkers, Jos
T1  - Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool
JF  - Stem Cells
Y1  - 2013
U6  - http://dx.doi.org/10.1002/stem.1437
SN  - 1549-4918
VL  - Vol 31
IS  - 9
SP  - 1910
EP  - 1920
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Gebeshuber, Christoph A.
A1  - Kornauth, Christoph
A1  - Dong, Lihua
A1  - Sierig, Ralph
A1  - Seibler, Jost
A1  - Reiss, Martina
A1  - Tauber, Stefanie
A1  - Bilban, Martin
A1  - Wang, Shijun
A1  - Kain, Renate
A1  - Böhmig, Georg A.
A1  - Moeller, Marcus J.
A1  - Gröne, Hermann-Josef
A1  - Englert, Christoph
A1  - Martinez, Javier
A1  - Kerjaschki, Dontscho
T1  - Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1
JF  - Nature Medicine
Y1  - 2013
U6  - http://dx.doi.org/10.1038/nm.3142
SN  - 1078-8956
VL  - 19
IS  - 4
SP  - 481
EP  - 487
ER  - 
TY  - JOUR
A1  - Kornfeld, Jan-Wilhelm
A1  - Baitzel, Catherina
A1  - Könner, A. Christine
A1  - Nicholls, Hayley T.
A1  - Vogt, Merly C.
A1  - Herrmanns, Karolin
A1  - Scheja, Ludger
A1  - Haumaitre, Cécile
A1  - Wolf, Anna M.
A1  - Knippschild, Uwe
A1  - Seibler, Jost
A1  - Cereghini, Silvia
A1  - Heeren, Joerg
A1  - Stoffel, Markus
A1  - Brüning, Jens C.
T1  - Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b
JF  - Nature
Y1  - 2013
SN  - 0028-0836
U6  - http://dx.doi.org/10.1038/nature11793
VL  - 494
IS  - 7435
SP  - 111
EP  - 115
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - JOUR
A1  - Inagaki, Akiko
A1  - Sleddens-Linkels, Esther
A1  - Wassenaar, Evelyne
A1  - Ooms, Marja
A1  - Cappellen, Wiggert A. van
A1  - Hoeijmakers, Jan H. J.
A1  - Seibler, Jost
A1  - Vogt, Thomas F.
A1  - Shin, Myung K.
A1  - Grootegoed, J. Anton
A1  - Baarends, Willy M.
T1  - Meiotic functions of RAD18
JF  - Journal of Cell Science
Y1  - 2011
U6  - http://dx.doi.org/10.1242/jcs.081968
SN  - 1477-9137
VL  - 124
IS  - 16
SP  - 2837
EP  - 2850
PB  - Company of Biologists Limited
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Schiffels, Johannes
A1  - Selmer, Thorsten
T1  - A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro
JF  - Biotechnology and Bioengineering
Y1  - 2015
U6  - http://dx.doi.org/10.1002/bit.25658
SN  - 1097-0290
VL  - 112
IS  - 11
SP  - 2360
EP  - 2372
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Sievers, Susanne
A1  - Becher, Dörte
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Schweder, Thomas
A1  - Maurer, Karl-Heinz
A1  - Hecker, Michael
T1  - High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium
JF  - Proteomics
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pmic.201400504
SN  - 1615-9861
VL  - 15
IS  - 15
SP  - 2629
EP  - 2633
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Molinnus, Denise
A1  - Bäcker, Matthias
A1  - Siegert, Petra
A1  - Willenberg, H.
A1  - Poghossian, Arshak
A1  - Keusgen, M.
A1  - Schöning, Michael Josef
T1  - Detection of Adrenaline Based on Substrate Recycling Amplification
JF  - Procedia Engineering
N2  - An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.708
SN  - 1877-7058
N1  - Eurosensors 2015
VL  - 120
SP  - 540
EP  - 543
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Pilas, Johanna
A1  - Mariano, K.
A1  - Keusgen, M.
A1  - Selmer, Thorsten
A1  - Schöning, Michael Josef
T1  - Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes
JF  - Procedia Engineering
Y1  - 2015
U6  - http://dx.doi.org/10.1016/j.proeng.2015.08.702
SN  - 1877-7058
N1  - Part of special issue "Eurosensors 2015"
VL  - 120
SP  - 532
EP  - 535
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Plum, Leona
A1  - Ma, Xiaosong
A1  - Hampel, Brigitte
A1  - Balthasar, Nina
A1  - Coppari, Roberto
A1  - Münzberg, Heike
A1  - Shanabrough, Marya
A1  - Burdakov, Denis
A1  - Rother, Eva
A1  - Janoschek, Ruth
A1  - Alber, Jens
A1  - Belgardt, Bengt F.
A1  - Koch, Linda
A1  - Seibler, Jost
A1  - Schenk, Frieder
A1  - Fekete, Csaba
A1  - Suzuki, Akira
A1  - Mak, Tak W.
A1  - Krone, Wilhelm
A1  - Horvath, Tamas L.
A1  - Ashcroft, Frances M.
A1  - Brüning, Jens C.
T1  - Enhanced PIP3 signaling in POMC neurons causes KATP channel activation and leads to diet-sensitive obesity
JF  - The Journal of Clinical Investigation (JCI)
Y1  - 2006
U6  - http://dx.doi.org/10.1172/JCI27123
SN  - 1558-8238
VL  - 116
IS  - 7
SP  - 1886
EP  - 1901
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Küter-Luks, Birgit
A1  - Kern, Heidrun
A1  - Streu, Sandra
A1  - Plum, Leona
A1  - Maurer, Jan
A1  - Kühn, Ralf
A1  - Brüning, Jens C.
A1  - Schwenk, Frieder
T1  - Single copy shRNA configuration for ubiquitous gene knockdown in mice
JF  - Nucleic Acids Research
Y1  - 2005
U6  - http://dx.doi.org/10.1093/nar/gni065
SN  - 1362-4962
VL  - 33
IS  - 7
SP  - e67
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Zevnik, Branko
A1  - Küter-Luks, Birgit
A1  - Andreas, Susanne
A1  - Kern, Heidrun
A1  - Hennek, Thomas
A1  - Rode, Anja
A1  - Heimann, Cornelia
A1  - Faust, Nicole
A1  - Kauselmann, Gunther
A1  - Schoor, Michael
A1  - Jaenisch, Rudolf
A1  - Rajewsky, Klaus
A1  - Kühn, Ralf
A1  - Schwenk, Frieder
T1  - Rapid generation of inducible mouse mutants
JF  - Nucleic Acids Research
Y1  - 2003
U6  - http://dx.doi.org/10.1093/nar/gng012
SN  - 1362-4962
VL  - 33
IS  - 4
SP  - e12
ER  - 
TY  - JOUR
A1  - Goetze, Sandra
A1  - Baer, Alexandra
A1  - Winkelmann, Silke
A1  - Nehlsen, Kristina
A1  - Seibler, Jost
A1  - Maass, Karin
A1  - Bode, Jürgen
T1  - Performance of genomic bordering elements at predefined genomic loci
JF  - Molecular and Cellular Biology
Y1  - 2005
U6  - http://dx.doi.org/10.1128/MCB.25.6.2260-2272.2005
SN  - 1098-5549
VL  - 25
IS  - 6
SP  - 2260
EP  - 2272
ER  - 
TY  - JOUR
A1  - Cesari, Francesca
A1  - Rennekampff, Verena
A1  - Vintersten, Kristina
A1  - Vuong, Lam Giang
A1  - Seibler, Jost
A1  - Bode, Jürgen
A1  - Wiebel, Franziska F.
A1  - Nordheim, Alfred
T1  - Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange
JF  - Genesis : The Journal of Genetics and Development
Y1  - 2004
U6  - http://dx.doi.org/10.1002/gene.20003
SN  - 1526-968X
VL  - 38
IS  - 2
SP  - 87
EP  - 92
ER  - 
TY  - JOUR
A1  - Takenaga, Shoko
A1  - Schneider, Benno
A1  - Erbay, E.
A1  - Biselli, Manfred
A1  - Schnitzler, Thomas
A1  - Schöning, Michael Josef
A1  - Wagner, Torsten
T1  - Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process
JF  - Physica status solidi (a)
N2  - A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.
Y1  - 2015
U6  - http://dx.doi.org/10.1002/pssa.201532053
SN  - 1862-6319
VL  - 212
IS  - 6
SP  - 1347
EP  - 1352
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Bode, Jürgen
A1  - Schlake, Thomas
A1  - Iber, Michaela
A1  - Schübeler, Dirk
A1  - Seibler, Jost
A1  - Snezhkov, Evgeney
A1  - Nikolaev, Lev
T1  - The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes
JF  - Biological Chemistry
Y1  - 2000
U6  - http://dx.doi.org/10.1515/BC.2000.103
SN  - 1431-6730
VL  - 381
IS  - 9-10
SP  - 801
EP  - 813
ER  - 
TY  - JOUR
A1  - Feng, Yong-Qing
A1  - Seibler, Jost
A1  - Alami, Raouf
A1  - Eisen, Andrew
A1  - Westerman, Karen A.
A1  - Leboulch, Philippe
A1  - Fiering, Steven
A1  - Bouhassira, Eric E.
T1  - Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange
JF  - Journal of Molecular Biology
Y1  - 1999
U6  - http://dx.doi.org/10.1006/jmbi.1999.3113
SN  - 0022-2836
VL  - 292
IS  - 4
SP  - 779
EP  - 785
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Schübeler, Dirk
A1  - Fiering, Steven
A1  - Groudine, Mark
A1  - Bode, Jürgen
T1  - DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs
JF  - Biochemistry
Y1  - 1998
U6  - http://dx.doi.org/10.1021/bi980288t
SN  - 1520-4995
VL  - 37
IS  - 18
SP  - 6229
EP  - 6234
ER  - 
TY  - JOUR
A1  - Seibler, Jost
A1  - Bode, Jürgen
T1  - Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay
JF  - Biochemistry
Y1  - 1997
SN  - 1520-4995
VL  - 36
IS  - 7
SP  - 1740
EP  - 1747
ER  - 
TY  - JOUR
A1  - Bode, J.
A1  - Bartsch, J.
A1  - Boulikas, T.
A1  - Iber, M.
A1  - Mielke, C.
A1  - Schübeler, D.
A1  - Seibler, Jost
A1  - Benham, C.
T1  - Transcription-promoting genomic sites in mammalia: their elucidation and architectural principles
JF  - Gene therapy & molecular biology
Y1  - 1998
SN  - 1529-9120
VL  - 1
IS  - 1
SP  - 1
EP  - 29
ER  - 
TY  - JOUR
A1  - Iber, Michaela
A1  - Schübeler, Dirk
A1  - Seibler, Jost
A1  - Höxter, Maria
A1  - Bode, Jürgen
T1  - Efficient FACS selection procedure for cells undergoing Flp-mediated site-specific conversions
JF  - Technical Tips Online
Y1  - 1998
U6  - http://dx.doi.org/10.1016/S1366-2120(08)70132-6
VL  - 4
IS  - 1
SP  - 25
EP  - 29
ER  - 
TY  - JOUR
A1  - Wiegand, Sandra
A1  - Voigt, Birgit
A1  - Albrecht, Dirk
A1  - Bongaerts, Johannes
A1  - Evers, Stefan
A1  - Hecker, Michael
A1  - Daniel, Rolf
A1  - Liesegang, Heiko
T1  - Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production
JF  - Microbial Cell Factories
Y1  - 2013
U6  - http://dx.doi.org/10.1186/1475-2859-12-120
SN  - 1475-2859
VL  - 12
SP  - 120
PB  - Biomed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Henken, F. E.
A1  - Oosterhuis, K.
A1  - Öhlschläger, Peter
A1  - Bosch, L.
A1  - Hooijberg, E.
A1  - Haanen, J. B. A. G.
A1  - Steenbergen, R. D. M.
T1  - Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7
JF  - Vaccine
N2  - Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.vaccine.2012.04.013
SN  - 0264-410X
VL  - 30
IS  - 28
SP  - 4259
EP  - 4266
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Immel, Timo
A1  - Grützke, Martin
A1  - Späte, Anne-Katrin
A1  - Groth, Ulrich
A1  - Öhlschläger, Peter
A1  - Huhn, Thomas
T1  - Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy
JF  - Chemical Communications
N2  - Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model.
Y1  - 2012
U6  - http://dx.doi.org/10.1039/C2CC31624B
SN  - 1364-548X
VL  - 48
IS  - 46
SP  - 5790
EP  - 5792
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - CHAP
A1  - Srivastava, Alok
A1  - Knolle, F.
A1  - Schnug, E.
A1  - Scherer, Ulrich W.
T1  - Study of Trace Elements in Water Bodies of the Harz Mts. Region, Germany using Total Reflection X Ray Fluorescence (TXRF)
T2  - DAE-BRNS 12th National Symposium on Nuclear and Radiochemistry NUCAR 2015, Feb. 9-13, Mumbai, India, Mumbai, India; 02/2015
Y1  - 2015
SP  - 355
EP  - 356
ER  - 
TY  - CHAP
A1  - Srivastava, Alok
A1  - Knolle, Friedhart
A1  - Hoyler, Friedrich
A1  - Scherer, Ulrich W.
A1  - Schnug, Ewald
T1  - Uranium Toxicity in the State of Punjab in North-Western India
T2  - Management of Natural Resources in a Changing Environment
N2  - Lately there has been an increasing concern about uranium toxicity in some districts of Punjab State located in the North Western part of India after the publication of a report (Blaurock-Busch et al. 2010) which showed that the concentration of uranium in hair and urine of children suffering from physical deformities, neurological and mental disorder from Malwa region (Fig. 1) of Punjab State was manifold higher than the reference ranges. A train which connects the affected region with the nearby city of Bikaner which has a Cancer Hospital has been nicknamed as Cancer Express due to the frenzy generated on account of uranium related toxicity.
Y1  - 2015
SN  - 978-3-319-12559-6
U6  - http://dx.doi.org/10.1007/978-3-319-12559-6_21
SP  - 271
EP  - 275
PB  - Springer
CY  - Cham
ER  - 
TY  - JOUR
A1  - Cehreli, Ruksan
A1  - Akpinar, Hale
A1  - Temiz Artmann, Aysegül
A1  - Sagol, Ozgul
T1  - Effects of Glutamine and Omega-3 Fatty Acids on Erythrocyte Deformability and Oxidative Damage in Rat Model of Enterocolitis
JF  - Gastroenterology Research
Y1  - 2015
U6  - http://dx.doi.org/10.14740/gr683w
SN  - 1918-2813
VL  - 8
IS  - 5
SP  - 265
EP  - 273
ER  - 
TY  - PAT
A1  - O'Connell, Timothy
A1  - Siegert, Petra
A1  - Maurer, Karl-Heinz
A1  - Schiedel, Marc-Steffen
A1  - Vockenroth, Inga Kerstin
T1  - Method for improving the cleaning action of a detergent or cleaning agent [Internationale Patentanmeldung]
T1  - Verfahren zur Verbesserung der Reinigungsleistung eines Wasch- oder Reinigungsmittels
Y1  - 2010
SP  - 1
EP  - 15
PB  - WIPO
CY  - Genf
ER  - 
TY  - CHAP
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, Ronald
A1  - Wagner, Torsten
T1  - Light-stimulated hydrogel actuators with incorporated graphene oxide for microfluidic applications
T2  - 12. Dresdner Sensor-Symposium 2015
Y1  - 2015
U6  - http://dx.doi.org/10.5162/12dss2015/P5.8
SP  - 206
EP  - 209
ER  - 
TY  - JOUR
A1  - Breuer, Lars
A1  - Raue, Markus
A1  - Strobel, M.
A1  - Mang, Thomas
A1  - Schöning, Michael Josef
A1  - Thoelen, R.
A1  - Wagner, Torsten
T1  - Hydrogels with incorporated graphene oxide as light-addressable actuator materials for cell culture environments in lab-on-chip systems
JF  - Physica status solidi (a)
N2  - Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.
Y1  - 2016
U6  - http://dx.doi.org/10.1002/pssa.201533056
SN  - 1862-6300
VL  - 213
IS  - 6
SP  - 1520
EP  - 1525
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Kasper, Katharina
A1  - Schiffels, Johannes
A1  - Krafft, Simone
A1  - Kuperjans, Isabel
A1  - Elbers, Gereon
A1  - Selmer, Thorsten
T1  - Biogas Production on Demand Regulated by Butyric Acid Addition
T2  - IOP Conference Series: Earth and Environmental Science. Bd. 32
Y1  - 2016
U6  - http://dx.doi.org/10.1088/1755-1315/32/1/012009
SN  - 1755-1315
N1  - ICARET 2016, International Conference on Advances in Renewable Energy and Technologies, Putrajaya, MY, Feb 23-25, 2016
VL  - 32
SP  - 012009/1
EP  - 012009/4
ER  -