TY  - JOUR
A1  - Zhubanova, Azhar A.
A1  - Aknazarov, S. K.
A1  - Mansurov, Zulkhair
A1  - Digel, Ilya
A1  - Kozhalakova, A. A.
A1  - Akimbekov, Nuraly Shardarbekovich
A1  - O'Heras, Carlos
A1  - Tazhibayeva, S.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials
N2  - Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.
KW  - Kohlenstofffaser
KW  - Lipopolysaccharide
KW  - nanostrukturierte carbonisierte Pflanzenteile
KW  - lipopolysaccharides
KW  - nanostructured carbonized plant parts
Y1  - 2010
ER  - 
TY  - JOUR
A1  - Zerlin, Kay
A1  - Kasischke, Nicole
A1  - Digel, Ilya
A1  - Maggakis-Kelemen, Christina
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Kayser, Peter
A1  - Linder, Peter
A1  - Artmann, Gerhard
T1  - Structural transition temperature of hemoglobins correlates with species’ body temperature
JF  - European Biophysics Journal. 37 (2007), H. 1
Y1  - 2007
SN  - 1432-1017
SP  - 1
EP  - 10
ER  - 
TY  - JOUR
A1  - Uysal, Karya
A1  - Firat, Ipek Serat
A1  - Creutz, Till
A1  - Aydin, Inci Cansu
A1  - Artmann, Gerhard
A1  - Teusch, Nicole
A1  - Temiz Artmann, Aysegül
T1  - A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes
JF  - membranes
N2  - Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here.
Y1  - 2022
U6  - http://dx.doi.org/10.3390/membranes13010022
N1  - This article belongs to the Special Issue "Latest Scientific Discoveries in Polymer Membranes"
VL  - 2023
IS  - 13(1)
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Uysal, Karya
A1  - Creutz, Till
A1  - Firat, Ipek Seda
A1  - Artmann, Gerhard
A1  - Teusch, Nicole
A1  - Temiz Artmann, Aysegül
T1  - Bio-functionalized ultra-thin, large-area and waterproof silicone membranes for biomechanical cellular loading and compliance experiments
JF  - Polymers
N2  - Biocompatibility, flexibility and durability make polydimethylsiloxane (PDMS) membranes top candidates in biomedical applications. CellDrum technology uses large area, <10 µm thin membranes as mechanical stress sensors of thin cell layers. For this to be successful, the properties (thickness, temperature, dust, wrinkles, etc.) must be precisely controlled. The following parameters of membrane fabrication by means of the Floating-on-Water (FoW) method were investigated: (1) PDMS volume, (2) ambient temperature, (3) membrane deflection and (4) membrane mechanical compliance. Significant differences were found between all PDMS volumes and thicknesses tested (p < 0.01). They also differed from the calculated values. At room temperatures between 22 and 26 °C, significant differences in average thickness values were found, as well as a continuous decrease in thicknesses within a 4 °C temperature elevation. No correlation was found between the membrane thickness groups (between 3–4 µm) in terms of deflection and compliance. We successfully present a fabrication method for thin bio-functionalized membranes in conjunction with a four-step quality management system. The results highlight the importance of tight regulation of production parameters through quality control. The use of membranes described here could also become the basis for material testing on thin, viscous layers such as polymers, dyes and adhesives, which goes far beyond biological applications.
Y1  - 2022
SN  - 2073-4360
VL  - 14
IS  - 11
SP  - 2213
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Trzewik, Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Linder, Peter
A1  - Demirci, T.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Evaluation of lateral mechanical tension in thin-film tissue constructs
JF  - Annals of Biomedical Engineering. 32 (2004), H. 9
Y1  - 2004
SN  - 1573-9686
SP  - 1243
EP  - 1251
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Yolcuoglu, B.
A1  - Onal, M. A.
A1  - Sipahi, O.
T1  - The effect of skin transplantation on tumour growth in mice. Yolcuoglu, B.; Onal, M. A.; Sipahi, O.; Temiz, A.; Pekcetin, C.; Acikgöz, O.; Kar,  H.; Güre A.
JF  - The American Journal of Surgery. 178 (1999), H. 4
Y1  - 1999
SN  - 0002-9610
SP  - 329
EP  - 330
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Yalcin, Ozlem
A1  - Resmi, Halil
A1  - Baskurt, Oguz K.
T1  - Can white blood cell activation be one of the major factors that affect hemorheological parameters during and after exercise?
JF  - Clinical Hemorheology and Microcirculation. 26 (2002), H. 3
Y1  - 2002
SN  - 1386-0291
N1  - = The 11th European Symposium on Clinical Hemorheology, Rouen, France, 19-22 September 2000
SP  - 189
EP  - 193
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Saklamaz, Ali
A1  - Comlekci, Abdurrahman
A1  - Caliskan, Sezer
T1  - The beneficial effects of lipid-lowering drugs beyond lipid-lowering effects: A comparative study with pravastatin, atorvastatin, and fenofibrate in patients with type IIa and type IIb hyperlipidemia / Saklamaz, Ali ; Comlekci, Abdurrahman ; Temiz, Aysegu
JF  - Metabolism. 54 (2005), H. 5
Y1  - 2005
SN  - 0026-0495
SP  - 677
EP  - 681
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Resmi, Halil
A1  - Akhunlar, Hülya
A1  - Güner, Gül
T1  - In vitro effects of high glucose concentrations on membrane protein sulfhydryl oxidation, G-actin and deformability of human erythrocytes. Resmi, Halil ; Akhunlar, Hülya ; Temiz Artmann, Aysegül ; Güner, Gül
JF  - Cell biochemistry and function. 23 (2005), H. 3
Y1  - 2005
SN  - 0263-6484
SP  - 163
EP  - 168
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Digel, Ilya
T1  - NMR in vitro effects on proliferation, apoptosis, and viability of human chondrocytes and osteoblasts
JF  - Methods and findings in Experimental and Clinical Pharmacology. 27 (2005), H. 6
Y1  - 2005
SN  - 0379-0355
SP  - 391
EP  - 394
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Kurulgan demirci, Eylem
A1  - Fırat, Ipek Seda
A1  - Oflaz, Hakan
A1  - Artmann, Gerhard
T1  - Recombinant activated protein C (rhAPC) affects lipopolysaccharide-induced mechanical compliance changes and beat frequency of mESC-derived cardiomyocyte monolayers
JF  - SHOCK
KW  - Septic cardiomyopathy
KW  - LPS
KW  - cardiomyocyte biomechanics
KW  - CellDrum
KW  - actin cytoskeleton
Y1  - 2021
U6  - http://dx.doi.org/10.1097/SHK.0000000000001845
SN  - 1540-0514
PB  - Wolters Kluwer
CY  - Köln
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Kayser, Peter
T1  - Why is Sepsis an Ongoing Clinical Challenge? Lipopolysaccharide Effects on Red Blood Cell Volume / Temiz, Aysegül ;  Kayser, Peter
JF  - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)
Y1  - 2008
SN  - 978-3-540-75408-4
SP  - 497
EP  - 508
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Kayatekin, Muammer B.
A1  - Uysal, N.
A1  - Resmi, H.
T1  - Does antioxidant supplementation alter the effects of acute exercise on erythrocyte aggregation, deformability and endothelium adhesion in untrained rats? Kayatekin, Muammer B.; Uysal N.; Resmi, H.; Bediz, Seref C.; Temiz Artmann, A.; Genç, S.; Tugyan, K.
JF  - International Journal for Vitamin and Nutrition Research. 75 (2005), H. 4
Y1  - 2005
SN  - 0300-9831
SP  - 243
EP  - 250
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Cavdar, C.
A1  - Yenicerioglu, Y.
A1  - Caliskan, S.
T1  - The effects of intravenous iron treatment on oxidant stress and erythrocyte deformability in haemodialysis patients. Cavdar, C.; Temiz, A.; Yenicerioglu, Y.; Caliskan, S.; Celik, A.; Sifil, A.; Onvural, B.; Camsari, T.
JF  - Scandinavian Journal of Urology and Nephrology. 37 (2003), H. 1
Y1  - 2003
SN  - 0036-5599
SP  - 77
EP  - 82
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Baskurt, Oguz K.
A1  - Pekcetin, C.
A1  - Kandemir, F.
T1  - Leukocyte activation, oxidant stress and red blood cell properties after acute, exhausting exercise in rats. Temiz, A.; Baskurt, O. K., Pekcetin, C.; Kandemir, F.; Güre, A.
JF  - Clinical Hemorheology and Microcirculation. 22 (2000), H. 4
Y1  - 2000
SN  - 1386-0291
SP  - 253
EP  - 259
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Baskurt, O. K.
A1  - Meiselman, H. J.
T1  - Effect of superoxide anions on red blood cell rheologic properties. Baskurt, O. K.; Temiz, A.; Meiselman, H. J.
JF  - Free Radical Biology and Medicine. 24 (1998), H. 1
Y1  - 1998
SN  - 0891-5849
SP  - 102
EP  - 110
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Baskurt, O. K.
A1  - Meiselman, H. J.
T1  - Red blood cell aggregation in experimental sepsis . Baskurt, O. K.; Temiz, A.; Meiselman, H. J.
JF  - Journal of Laboratory and Clinical Medicine. 130 (1997), H. 2
Y1  - 1997
SN  - 0022-2143
SP  - 183
EP  - 190
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Baskurt, O. K.
A1  - Edremitlioglu, M.
T1  - Effect of erythrocyte deformability on myocardial hematocrit gradient. Baskurt, O.K.; Edremitlioglu, M.; Temiz, A.
JF  - American Journal of Physiology: Heart and Circulatory Physiology. 268 (1995), H. 1
Y1  - 1995
SN  - 0363-6135
SP  - 260
EP  - 264
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Baskurt, O. K.
A1  - Edremitlioglu, M.
T1  - In vitro effects of in vivo activated leukocytes on red blood cell filterability and lipid peroxidation. Baskurt, O.K.; Edremitlioglu, M.; Temiz, A.
JF  - Clinical Hemorheology. 14 (1994), H. 4
Y1  - 1994
SP  - 591
EP  - 596
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Ates, H.
A1  - Üretmen, Ö.
A1  - Andac, K.
T1  - Erythrocyte deformability in high-tension and normal tension glaucoma. Ates, H.; Üretmen, Ö.; Temiz, A.; Andac, K.
JF  - International Ophthalmology. 22 (1998), H. 1
Y1  - 1998
SN  - 0165-5701
SP  - 7
EP  - 12
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Akhisaroglu, M.
A1  - Sercan, Z.
A1  - Kayatekin, B. M.
T1  - Adhesion of Erythrocytes to Endothelial Cells After Acute Exercise: Differences in Red Blood Cells from Juvenile and Adult Rats. Temiz Artmann, A.; Akhisaroglu, M.; Sercan, Z.; Kayatekin, BM.; Yorukoglu, K.; Kirkali, G.
JF  - Physiological  Research (2005)
Y1  - 2005
SN  - 0862-8408
N1  - pre-press article
ER  - 
TY  - THES
A1  - Temiz Artmann, Aysegül
T1  - Sicanlarda egzersiz sonrasi oksidatif hasar ve eritrosit membran degisikliklerinin hemoreolojik etkileri
T1  - The effect of oxidative stress and erythrocyte membrane changes on hemorheological factors following exercise in rats
Y1  - 2001
N1  - Dokuz Eylül Üniversitesi, Izmir, Diss., 2001. 
Veröffentlicht unter Temiz, Aysegül
ER  - 
TY  - THES
A1  - Temiz Artmann, Aysegül
T1  - Sicanlarda, akut egzersiz sonucu gelisen oksidan stresin lökosit aktivasyon degisiklikleri ile ile olan iliskisi ve eritrosit deformabilitesine etkisi
T1  - The oxidative stress effect on leukocyte activation and erythrocyte deformability after acute exercise in rats
Y1  - 1996
N1  - Dokuz Eylül Üniversitesi, Izmir, Diss., 1996.
Veröffentlicht unter Temiz, Ayşegül
ER  - 
TY  - RPRT
A1  - Temiz Artmann, Aysegül
T1  - Frühgeburtenrate mindern durch ein Prognoseverfahren für den vorzeitigen Blasensprung - PROMPT (Premature rupture of membranes prediction test) : Abschlussbericht ; Laufzeit des Vorhabens: 01.03.2007 - 31.12.2009
Y1  - 2010
U6  - http://dx.doi.org/10.2314/GBV:644277858
N1  - Förderkennzeichen BMBF 1772X07
PB  - Technische Informationsbibliothek u. Universitätsbibliothek
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Seifarth, Volker
A1  - Schehl, D.
A1  - Linder, Peter
A1  - Gossmann, Matthias
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Porst, Dariusz
A1  - Preiß, C.
A1  - Kayser, Peter
A1  - Pack, O.
A1  - Temiz Artmann, Aysegül
T1  - Ureplace: development of a bioreactor for in vitro culturing of cell seeded tubular vessels on collagen scaffolds
N2  - The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 %. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days.
KW  - Tissue Engineering
KW  - Bioreaktor
KW  - Organkultur
KW  - Harnleiter
Y1  - 2011
ER  - 
TY  - JOUR
A1  - Seifarth, Volker
A1  - Grosse, Joachim O.
A1  - Grossmann, Matthias
A1  - Janke, Heinz Peter
A1  - Arndt, Patrick
A1  - Koch, Sabine
A1  - Epple, Matthias
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Mechanical induction of bi-directional orientation of primary porcine bladder smooth muscle cells in tubular fibrin-poly(vinylidene fluoride) scaffolds for ureteral and urethral repair using cyclic and focal balloon catheter stimulation
JF  - Journal of Biomaterials Applications
Y1  - 2017
U6  - http://dx.doi.org/10.1177/0885328217723178
SN  - 1530-8022
VL  - 32
IS  - 3
SP  - 321
EP  - 330
PB  - Sage
CY  - London
ER  - 
TY  - JOUR
A1  - Seifarth, Volker
A1  - Goßmann, Matthias
A1  - Grosse, J. O.
A1  - Becker, C.
A1  - Heschel, I.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds
JF  - Urologia Internationalis
Y1  - 2015
U6  - http://dx.doi.org/10.1159/000368419
SN  - 0042-1138
VL  - 2015
IS  - 95
SP  - 106
EP  - 113
PB  - Karger
CY  - Basel
ER  - 
TY  - JOUR
A1  - Sadykov, Rustam
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Artmann, Gerhard
A1  - Savitskaya, Irina
A1  - Zhubanova, Azhar
T1  - Oral lead exposure induces dysbacteriosis in rats
JF  - Journal of Occupational Health. 51 (2009) (2009), H. 1
Y1  - 2009
SN  - 1348-9585
SP  - 64
EP  - 73
ER  - 
TY  - JOUR
A1  - Preiß, C.
A1  - Linder, Peter
A1  - Wendt, K.
A1  - Krystek, M.
A1  - Digel, Ilya
A1  - Gossmann, Matthias
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Kayser, Peter
A1  - Bassam, Rasha
A1  - Artmann, Gerhard
T1  - Engineering technology for plant physiology and plant stress research
N2  - Plant physiology and plant stress: Plant physiology will be much more important for human mankind because of yield and cultivation limits of crops determined by their resistance to stress. To assess and counteract various stress factors it is necessary to conduct plant research to gain information and results on plant physiology.
KW  - Pflanzenphysiologie
KW  - Pflanzenstress
KW  - Pflanzenscanner
KW  - plant stress
KW  - plant scanner
Y1  - 2011
ER  - 
TY  - CHAP
A1  - O\'Heras, Carlos
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - Nanostructured carbon-based column for LPS/protein adsorption : [abstract]
N2  - The absence of a general method for endotoxin removal from liquid interfaces gives an opportunity to find new methods and materials to overcome this gap. Activated nanostructured carbon is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study is to find the adsorption rates for a carboneous material produced at different temperatures, as well as to reveal possible differences between the performance of the material for each of the adsorbates used during the study (hemoglobin, serum albumin and lipopolysaccharide, LPS).
KW  - Kohlenstofffaser
KW  - Adsorption
KW  - Lipopolysaccharide
KW  - aktivierte nanostrukturierte Kohlenstofffaser
KW  - lipopolysaccharides
KW  - activated nanostructured carbon
Y1  - 2009
ER  - 
TY  - JOUR
A1  - Niedermeyer, Angela
A1  - Zhou, Bei
A1  - Dursun, Gözde
A1  - Temiz Artmann, Aysegül
A1  - Markert, Bernd
T1  - An examination of tissue engineered scaffolds in a bioreactor
JF  - Proceedings in Applied Mathematics and Mechanics PAMM
N2  - Replacement tissues, designed to fill in articular cartilage defects, should exhibit the same properties as the native material. The aim of this study is to foster the understanding of, firstly, the mechanical behavior of the material itself and, secondly, the influence of cultivation parameters on cell seeded implants as well as on cell migration into acellular implants. In this study, acellular cartilage replacement material is theoretically, numerically and experimentally investigated regarding its viscoelastic properties, where a phenomenological model for practical applications is developed. Furthermore, remodeling and cell migration are investigated.
Y1  - 2016
U6  - http://dx.doi.org/10.1002/pamm.201610038
SN  - 1617-7061
N1  - Joint Annual Meeting of DMV and GAMM 2016, 87th Annual Meeting of the International Association of Applied Mathematics and Mechanics (GAMM) and Deutsche Mathematiker-Vereinigung (DMV), Braunschweig, DE, Mar 7-11, 2016
VL  - 16
IS  - 1
SP  - 99
EP  - 100
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Micili, Serap C.
A1  - Valter, Markus
A1  - Oflaz, Hakan
A1  - Ozogul, Candan
A1  - Linder, Peter
A1  - Föckler, Nicole
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - Optical coherence tomography : a potential tool to predict premature rupture of fetal membranes
JF  - Proceedings of the Institution of Mechanical Engineers. Part H : Journal of engineering in medicine
Y1  - 2013
SN  - 0046-2039 (Print) ; 2041-3033 (E-Journal)
VL  - Vol. 227
IS  - No. 4
SP  - 393
EP  - 401
PB  - Sage
CY  - London
ER  - 
TY  - CHAP
A1  - Mansurov, Zulkhair
A1  - Zhubanova, Azhar A.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
A1  - Savitskaja, Irina S.
A1  - Kozhalakova, A. A.
A1  - Kistaubaeva, Aida S.
T1  - The sorption of LPS toxic shock by nanoparticles on base of carbonized vegetable raw materials
N2  - Immobilization of lactobacillus on high temperature carbonizated vegetable raw material (rice husk, grape stones) increases their physiological activity and the quantity of the antibacterial metabolits, that consequently lead to increase of the antagonistic activity of lactobacillus. It is implies that the use of the nanosorbents for the attachment of the probiotical microorganisms are highly perspective for decision the important problems, such as the probiotical preparations delivery to the right address and their attachment to intestines mucosa with the following detoxication of gastro-intestinal tract and the normalization of it’s microecology. Besides that, thus, the received carbonizated nanoparticles have peculiar properties – ability to sorption of LPS toxical shock and, hence, to the detoxication of LPS.
KW  - Kohlenstofffaser
KW  - Lipopolysaccharide
KW  - nanostrukturierte carbonisierte Pflanzenteile
KW  - lipopolysaccharides
KW  - nanostructured carbonized plant parts
Y1  - 2008
ER  - 
TY  - JOUR
A1  - Malik, A. M.
A1  - Abdieva, G. Zh.
A1  - Ualieva, P. S.
A1  - Zhubanova, A. A.
A1  - Temiz Artmann, Aysegül
T1  - CКPИНИНГ МИКPOOPГAНИЗМOВ-ДECТPУКТOPOВ XЛOРOPГAНИЧECКИX ЗAГPЯЗНИТEЛEЙ
T1  - Screening of microorganisms – destructors of chlororganic pollutants
JF  - Eurasian Journal of Ecology
Y1  - 2019
SN  - 2617-7358
VL  - 61
IS  - 4
SP  - 61
EP  - 71
ER  - 
TY  - JOUR
A1  - Linder, Peter
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Kayser, Peter
A1  - Porst, Dariusz
A1  - Artmann, Gerhard
T1  - High-throughput testing of mechanical forces generated in thin cell and tissue layers
JF  - Tissue Engineering. 13 (2007), H. 7
Y1  - 2007
SN  - 1076-3279
N1  - Meeting abstract 433
SP  - 1778
EP  - 1778
ER  - 
TY  - JOUR
A1  - Leimena, W.
A1  - Artmann, Gerhard
A1  - Dachwald, Bernd
A1  - Temiz Artmann, Aysegül
A1  - Gossmann, Matthias
A1  - Digel, Ilya
T1  - Feasibility of an in-situ microbial decontamination of an ice-melting probe
JF  - Eurasian Chemico-Technological Journal. 12 (2010), H. 2
Y1  - 2010
SN  - 1562-3920
SP  - 145
EP  - 150
ER  - 
TY  - JOUR
A1  - Kurz, R.
A1  - Linder, Peter
A1  - Trzewik, Jürgen
A1  - Rüffer, M.
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Rothermel, A.
A1  - Robitzki, A.
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes
JF  - Medical and Biological Engineering and Computing
N2  - The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.
Y1  - 2010
U6  - http://dx.doi.org/10.1007/s11517-009-0552-y
SN  - 1741-0444
VL  - 48
IS  - 1
SP  - 59
EP  - 65
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Demirci, Taylan
A1  - Trzewik, Jürgen
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension of endothelial cells: An LPS based in-vitro sepsis model
JF  - IUBMB Life. 61 (2009), H. 3
Y1  - 2009
SN  - 1521-6543
N1  - Abstracts: Turkish Society of Molecular Medicine, Third International Congress of Molecular Medicine, May 5-8, 2009, Istanbul, Turkey
SP  - 307
EP  - 308
PB  - Wiley
CY  - Weinheim
ER  - 
TY  - CHAP
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Demirci, Taylan
A1  - Gierkowski, Jessica R.
A1  - Digel, Ilya
A1  - Gossmann, Matthias
A1  - Temiz Artmann, Aysegül
T1  - rhAPC reduces the endothelial cell permeability via a decrease of cellular mechanical contractile tensions : [abstract]
N2  - In this study, the CellDrum technology quanitfying cellular mechanical tension on a pico-scale was used to investigate the effect of LPS (lipopolysaccharide) on HAoEC (Human Aortic Endothelial Cell) tension.
KW  - Endothelzelle
KW  - Sepsis
KW  - kontraktile Spannung
KW  - rhAPC
KW  - contractile tension
KW  - rhAPC
KW  - celldrum technology
Y1  - 2010
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Demirci, Taylan
A1  - Linder, Peter
A1  - Trzewik, Jürgen
A1  - Gierkowski, Jessica Ricarda
A1  - Gossmann, Matthias
A1  - Kayser, Peter
A1  - Porst, Dariusz
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - rhAPC reduces the endothelial cell permeability via a decrease of contractile tensions induced by endothelial cells
JF  - Journal of Bioscience and Bioengineering
N2  - All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.
KW  - Cell permeability
KW  - Cellular force
KW  - Endothelial cells
KW  - Recombinant activated protein C
KW  - Lipopolysaccharide
KW  - Contractile tension
KW  - CellDrum
Y1  - 2012
U6  - http://dx.doi.org/10.1016/j.jbiosc.2012.03.019
SN  - 1347-4421
VL  - 113
IS  - 2
SP  - 212
EP  - 219
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Kurulgan Demirci, Eylem
A1  - Demirci, T.
A1  - Trzewik, Jürgen
A1  - Linder, Peter
A1  - Karakulah, G.
A1  - Artmann, Gerhard
A1  - Sakizli, M.
A1  - Temiz Artmann, Aysegül
T1  - Genome-Wide Gene Expression Analysis of NIH 3T3 Cell Line Under Mechanical Stimulation
JF  - Cellular and molecular bioengineering. 4 (2011), H. 1
Y1  - 2011
SN  - 1865-5025
SP  - 46
EP  - 55
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Kozhalakova, A. A.
A1  - Zhubanova, Azhar A.
A1  - Mansurov, Z. A.
A1  - Digel, Ilya
A1  - Tazhibayeva, S. M.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Adsorption of bacterial lipopolysaccharides on carbonized rice shell
JF  - Science of Central Asia (2010)
Y1  - 2010
SP  - 50
EP  - 54
ER  - 
TY  - JOUR
A1  - Goßmann, Matthias
A1  - Frotscher, Ralf
A1  - Linder, Peter
A1  - Bayer, Robin
A1  - Epple, U.
A1  - Staat, Manfred
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
T1  - Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells
JF  - Cellular physiology and biochemistry
N2  - Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.
KW  - Inotropic compounds
KW  - Pharmacology
KW  - Ion channels
KW  - CellDrum
KW  - Heart tissue culture
KW  - Induced pluripotent stem cells
KW  - Cardiac myocytes
Y1  - 2016
U6  - http://dx.doi.org/10.1159/000443124
SN  - 1421-9778 (Online)
SN  - 1015-8987 (Print)
VL  - 38
IS  - 3
SP  - 1182
EP  - 1198
PB  - Karger
CY  - Basel
ER  - 
TY  - JOUR
A1  - Frotscher, Ralf
A1  - Muanghong, Danita
A1  - Dursun, Gözde
A1  - Goßmann, Matthias
A1  - Temiz Artmann, Aysegül
A1  - Staat, Manfred
T1  - Sample-specific adaption of an improved electro-mechanical model of in vitro cardiac tissue
JF  - Journal of Biomechanics
N2  - We present an electromechanically coupled computational model for the investigation of a thin cardiac tissue construct consisting of human-induced pluripotent stem cell-derived atrial, ventricular and sinoatrial cardiomyocytes. The mechanical and electrophysiological parts of the finite element model, as well as their coupling are explained in detail. The model is implemented in the open source finite element code Code_Aster and is employed for the simulation of a thin circular membrane deflected by a monolayer of autonomously beating, circular, thin cardiac tissue. Two cardio-active drugs, S-Bay K8644 and veratridine, are applied in experiments and simulations and are investigated with respect to their chronotropic effects on the tissue. These results demonstrate the potential of coupled micro- and macroscopic electromechanical models of cardiac tissue to be adapted to experimental results at the cellular level. Further model improvements are discussed taking into account experimentally measurable quantities that can easily be extracted from the obtained experimental results. The goal is to estimate the potential to adapt the presented model to sample specific cell cultures.
KW  - hiPS cardiomyocytes
KW  - Homogenization
KW  - Hodgkin–Huxley models
KW  - Frequency adaption
KW  - Electromechanical modeling
KW  - Drug simulation
KW  - Computational biomechanics
KW  - Cardiac tissue
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.jbiomech.2016.01.039
SN  - 0021-9290 (Print)
SN  - 1873-2380 (Online)
VL  - 49
IS  - 12
SP  - 2428
EP  - 2435
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Frotscher, Ralf
A1  - Goßmann, Matthias
A1  - Temiz Artmann, Aysegül
A1  - Staat, Manfred
T1  - Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM
T2  - 1st International Conference "Shell and Membrane Theories in Mechanics and Biology: From Macro- to Nanoscale Structures", Minsk, Belarus, Sept. 16-20, 2013
Y1  - 2013
SN  - 978-985-553-135-8
SP  - 165
EP  - 167
PB  - Verl. d. Weißruss. Staatl. Univ.
CY  - Minsk
ER  - 
TY  - CHAP
A1  - Frotscher, Ralf
A1  - Goßmann, Matthias
A1  - Raatschen, Hans-Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Staat, Manfred
T1  - Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM
T2  - Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45)
N2  - We present an electromechanically coupled Finite Element model for cardiac tissue. It bases on the mechanical model for cardiac tissue of Hunter et al. that we couple to the McAllister-Noble-Tsien electrophysiological model of purkinje fibre cells. The corresponding system of ordinary differential equations is implemented on the level of the constitutive equations in a geometrically and physically nonlinear version of the so-called edge-based smoothed FEM for plates. Mechanical material parameters are determined from our own pressure-deflection experimental setup. The main purpose of the model is to further examine the experimental results not only on mechanical but also on electrophysiological level down to ion channel gates. Moreover, we present first drug treatment simulations and validate the model with respect to the experiments.
Y1  - 2015
SN  - 978-3-319-02534-6 ; 978-3-319-02535-3
SP  - 187
EP  - 212
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Erbayraktar, Zubeyde
A1  - Yilmaz, Osman
A1  - Temiz Artmann, Aysegül
A1  - Cehreli, Ruksan
A1  - Coker, Canan
T1  - Effects of Selenium Supplementation on Antioxidant Defense and Glucose Homeostasis in Experimental Diabetes Mellitus
JF  - Biological Trace Element Research
Y1  - 2007
SN  - 1559-0720
VL  - 118
IS  - 3
SP  - 217
EP  - 226
ER  - 
TY  - CHAP
A1  - Duong, Minh Tuan
A1  - Seifarth, Volker
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
A1  - Staat, Manfred
ED  - Artmann, Gerhard
ED  - Temiz Artmann, Aysegül
ED  - Zhubanova, Azhar A.
ED  - Digel, Ilya
T1  - Growth Modelling Promoting Mechanical Stimulation of Smooth Muscle Cells of Porcine Tubular Organs in a Fibrin-PVDF Scaffold
T2  - Biological, Physical and Technical Basics of Cell Engineering
N2  - Reconstructive surgery and tissue replacements like ureters or bladders reconstruction have been recently studied, taking into account growth and remodelling of cells since living cells are capable of growing, adapting, remodelling or degrading and restoring in order to deform and respond to stimuli. Hence, shapes of ureters or bladders and their microstructure change during growth and these changes strongly depend on external stimuli such as training. We present the mechanical stimulation of smooth muscle cells in a tubular fibrin-PVDFA scaffold and the modelling of the growth of tissue by stimuli. To this end, mechanotransduction was performed with a kyphoplasty balloon catheter that was guided through the lumen of the tubular structure. The bursting pressure was examined to compare the stability of the incubated tissue constructs. The results showed the significant changes on tissues with training by increasing the burst pressure as a characteristic mechanical property and the smooth muscle cells were more oriented with uniformly higher density. Besides, the computational growth models also exhibited the accurate tendencies of growth of the cells under different external stimuli. Such models may lead to design standards for the better layered tissue structure in reconstructing of tubular organs characterized as composite materials such as intestines, ureters and arteries.
KW  - Mechanical simulation
KW  - Growth modelling
KW  - Ureter
KW  - Bladder
KW  - Reconstruction
Y1  - 2018
SN  - 978-981-10-7904-7
U6  - http://dx.doi.org/10.1007/978-981-10-7904-7_9
SP  - 209
EP  - 232
PB  - Springer
CY  - Singapore
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Zerlin, Kay
A1  - Temiz Artmann, Aysegül
A1  - Engels, S.
T1  - Protein dynamics in thermosensation
JF  - Regenerative medicine. 2 (2007), H. 5
Y1  - 2007
SN  - 1746-0751
N1  - Proceedings of the 3rd World Congress on Regenerative Medicine. October 18-20, 2007. Leipzig, Germany
SP  - 533
EP  - 533
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Trzewik, Jürgen
A1  - Demirci, Taylan
A1  - Temiz Artmann, Aysegül
T1  - Response of fibroblasts to cyclic mechanical stress : a proteome approach / Digel, I. ; Trzewik, J. ; Demirci, T. ; Temiz Artmann, A. ; Artmann, G. M.
JF  - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2
Y1  - 2004
SN  - 0932-4666
SP  - 1042
EP  - 1043
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - Effects of plasma generated ions on bacteria : [poster]
N2  - Summary and Conclusions PCIs were clearly effective in terms of their antibacterial effects with the strains tested. This efficacy increased with the time the bacteries were exposed to PCIs. The bactericidal action has proved to be irreversible. PCIs were significantly less effective in shadowed areas. PCI exposure caused multiple protein damages as observed in SDS PAGE studies. There was no single but multiple molecular mechanism causing the bacterial death.
KW  - Clusterion
KW  - Bakterien
KW  - plasma generated ions
Y1  - 2003
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - Some peculiarities of application of cluster ions generated by plasma in respect of indoor air purification :[abstract]
N2  - Recently, the SHARP Corporation, Japan, has developed the world’s first "Plasma Cluster Ions (PCI)" air purification technology using plasma discharge to generate cluster ions. The new plasma cluster device releases positive and negative ions into the air, which are able to decompose and deactivate harmful airborne substances by chemical reactions. Because cluster ions consist of positive and negative ions that normally exist in the natural world, they are completely harmless and safe to humans. The amount of ozone generated by cluster ions is less than 0.01 ppm, which is significantly less than the 0.05-ppm standard for industrial operations and consumer electronics. This amount, thus, has no harming effects whatsoever on the human body. But particular properties and chemical processes in PCI treatment are still under study. It has been shown that PCI in most cases show strongly pronounced irreversible killing effects in respect of airborne microflora due to free-radical induced reactions and can be considered as a potent technology to disinfect both home, medical and industrial appliances.
KW  - Clusterion
KW  - Raumluft
KW  - Luftreiniger
KW  - Plasmacluster ion technology
KW  - Air purification
Y1  - 2003
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - Plasma-generated cluster ions' effects on indoor microflora : [abstract]
N2  - Recently, SHARP corporation has developed the world’s first "Plasma Cluster Ions® (PCI)" air purification technology, which uses plasma discharge to generate cluster ions. The new Plasma Cluster Device releases positive and negative ions into the air, which are harmless to humans and are able to decompose and deactivate airborne substances by chemical reactions. In the past, phenomenological tests on the efficacy of the PCI air purification technology on microbial cells have been conducted. In most cases, it has been shown that PCI demonstrated strongly pronounced killing effects on microorganisms. However, the particular mechanisms of PCI action still have to be uncovered.
KW  - Clusterion
KW  - Raumluft
KW  - Luftreiniger
KW  - Plasmacluster ion technology
KW  - Air purification
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Nishikawa, K.
A1  - Cook, M.
T1  - Bactericidal effects of plasma-generated cluster ions
JF  - Medical and Biological Engineering and Computing. 43 (2005), H. 6
Y1  - 2005
SN  - 1741-0444
SP  - 800
EP  - 807
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Nishikawa, K.
A1  - Artmann, Gerhard
T1  - Cluster air-ion effects on bacteria and moulds
JF  - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2
Y1  - 2004
SN  - 0932-4666
SP  - 1040
EP  - 1041
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
A1  - Nojima, H.
T1  - Plasma cluster ions (PCI) influence on microbial cells viability : [abstract]
N2  - Recently, SHARP corporation has developed the world’s first “Plasma Cluster Ions (PCI)” air purification technology, which uses plasma discharge to generate cluster ions. The new plasma cluster device releases into the air positive and negative ions, which are harmless to humans and are able to decompose and deactivate airborne substances by chemical reactions. A lot of phenomenological tests of the PCI air purification technology on microbial cells have been conducted. And, in most cases, it has been shown that PCI demonstrate strongly pronounced killing effect. Although, the particular mechanisms of PCI action are still not evident. We studied variations in resistance to PCI among gram-positive airborne microorganisms, as well as some dose-dependent, spatial, cultural and biochemical properties of PCI action in respect of Staphylococcus spp, Enterococcus spp, Micrococcus spp.
KW  - Clusterion
KW  - Raumluft
KW  - Luftreiniger
KW  - Plasmacluster ion technology
KW  - Air purification
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - The emperor's new body : seeking for a blueprint of limb regeneration in humans
JF  - Stem cell engineering : principles and applications / Gerhard M. Artmann ... eds.
Y1  - 2011
SN  - 978-3-642-11864-7
SP  - 3
EP  - 37
PB  - Springer
CY  - Berlin [u.a.]
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Sadykov, R.
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
T1  - Changes in intestinal microflora in rats induced by oral exposure to low lead (II) concentrations
T2  - Lead Exposure and Poisoning: Clinical Symptoms, Medical Management and Preventive Strategies
Y1  - 2015
SN  - 9781634826990
SP  - 75
EP  - 99
PB  - Nova Science Publ.
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Leimena, W.
A1  - Dachwald, Bernd
A1  - Linder, Peter
A1  - Porst, Dariusz
A1  - Kayser, Peter
A1  - Funke, O.
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
T1  - In-situ biological decontamination of an ice melting probe : [abstract]
N2  - The objective of our study was to investigate the efficacy of different in-situ decontamination protocols in the conditions of thermo-mechanical ice-melting.
KW  - Sonde
KW  - Dekontamination
KW  - Wasserstoffperoxid
KW  - Natriumhypochlorit
Y1  - 2010
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Demirci, Taylan
A1  - Trzewik, Jürgen
A1  - Linder, Peter
A1  - Temiz Artmann, Aysegül
T1  - Fibroblast response to mechanical stress: role of the adhesion substrate : [abstract]
N2  - Mechanical stimulation of the cells resulted in evident changes in the cell morphology, protein composition and gene expression. Microscopically, additional formation of stress fibers accompanied by cell re-arrangements in a monolayer was observed. Also, significant activation of p53 gene was revealed as compared to control. Interestingly, the use of CellTech membrane coating induced cell death after mechanical stress had been applied. Such an effect was not detected when fibronectin had been used as an adhesion substrate.
KW  - Fibroblast
KW  - Mechanische Beanspruchung
KW  - celldrum technology
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Demirci, Taylan
A1  - Temiz Artmann, Aysegül
A1  - Nishikawa, K.
T1  - Free Radical Nature of the Bactericidal Effect of Plasma-Generated Cluster Ions (PCIs)
JF  - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2
Y1  - 2004
SN  - 0932-4666
SP  - 982
EP  - 983
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Akimbekov, N.
A1  - Turalieva, M.
A1  - Mansurov, Z.
A1  - Temiz Artmann, Aysegül
A1  - Eshibaev, A.
A1  - Zhubanova, A.
T1  - Usage of Carbonized Plant Wastes for Purification of Aqueous Solutions
JF  - Journal of Industrial Technology and Engineering
Y1  - 2013
VL  - 2
IS  - 07
SP  - 47
EP  - 54
ER  - 
TY  - JOUR
A1  - Demirci, Taylan
A1  - Kurulgan Demirci, Eylem
A1  - Trzewik, Jürgen
A1  - Linder, Peter
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Sakizli, Meral
A1  - Temiz Artmann, Aysegül
T1  - Gene expression profile analysis of 3T3/NIH fibroblasts after one hour mechanical stress
JF  - IUBMB Life. 61 (2009), H. 3
Y1  - 2009
SN  - 1521-6543
N1  - Abstracts: Turkish Society of Molecular Medicine, Third International Congress of Molecular Medicine, May 5-8, 2009, Istanbul, Turkey
SP  - 311
EP  - 312
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Demirci, T.
A1  - Trzewik, J.
A1  - Linder, Peter
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Mechanical Stimulation of 3T3 Fibroblasts Activates Genes: ITGB5 and p53 Responses as Quantified on the mRNA Level
JF  - Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2
Y1  - 2004
SN  - 0932-4666
SP  - 1030
EP  - 1031
ER  - 
TY  - JOUR
A1  - Demirci, T.
A1  - Trzewik, J.
A1  - Linder, Peter
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Mechanical Stimulation of 3T3 Fibroblasts Activates Genes: Real Time PCR Products and Suppliers by Comparison
JF  - Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2
Y1  - 2004
SN  - 0932-4666
SP  - 1046
EP  - 1047
ER  - 
TY  - JOUR
A1  - Cehreli, Ruksan
A1  - Akpinar, Hale
A1  - Temiz Artmann, Aysegül
A1  - Sagol, Ozgul
T1  - Effects of Glutamine and Omega-3 Fatty Acids on Erythrocyte Deformability and Oxidative Damage in Rat Model of Enterocolitis
JF  - Gastroenterology Research
Y1  - 2015
U6  - http://dx.doi.org/10.14740/gr683w
SN  - 1918-2813
VL  - 8
IS  - 5
SP  - 265
EP  - 273
ER  - 
TY  - JOUR
A1  - Bayer, Robin
A1  - Temiz Artmann, Aysegül
A1  - Digel, Ilya
A1  - Falkenstein, Julia
A1  - Artmann, Gerhard
A1  - Creutz, Till
A1  - Hescheler, Jürgen
T1  - Mechano-pharmacological testing of L-Type Ca²⁺ channel modulators via human vascular celldrum model
JF  - Cellular Physiology and Biochemistry
N2  - Background/Aims: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology. Methods: The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. The cell generated tensions deviations in the range of a few N/m² are evaluated by the CellDrum technology. This study focuses on the dilative and contractive effects of L-type Ca²⁺ channel agonists and antagonists, respectively. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate analysis technique for the study purpose. These three operation modes are called, particular time mode" (PTM), "long term mode" (LTM) and "real-time mode" (RTM). Results: It was possible to quantify the biomechanical response of haSMCs to the addition of vasoactive agents using CellDrum technology. Due to the supplementation of 100nM Bay K8644, the tension increased approximately 10.6% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomechanical stress around 6,5% and 50nM verapamil by 2,8%, compared to the initial tension maximum. Additionally, all tested measurement modes provide similar results while focusing on different analysis parameters. Conclusion: The CellDrum technology allows highly sensitive biomechanical stress measurements of cultured haSMC monolayers. The mechanical stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m²). All tested operation modes resulted in equal findings, whereas each mode features operation-related data analysis.
Y1  - 2020
U6  - http://dx.doi.org/10.33594/000000225
SN  - 1421-9778
VL  - 54
SP  - 371
EP  - 383
PB  - Cell Physiol Biochem Press
CY  - Düsseldorf
ER  - 
TY  - CHAP
A1  - Bayer, Robin
A1  - Hescheler, Jürgen
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
ED  - Staat, Manfred
ED  - Erni, Daniel
T1  - Treating arterial hypertension in a cell culture well
T2  - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH AachenW
N2  - Hypertension describes the pathological increase of blood pressure, which is most commonly associated with the increase of vascular wall stiffness [1]. Referring to the “Deutsche Bluthochdruck Liga” this pathology shows a growing trend in our aging society. In order to find novel pharmacological and probably personalized treatments, we want to present a functional approach to study biomechanical properties of a human aortic vascular model.
In this method review we will give an overview of recent studies which were carried out with the CellDrum technology [2] and underline the added value to already existing standard procedures known from the field of physiology.
Herein described CellDrum technology is a system to measure functional mechanical properties of cell monolayers and thin tissue constructs in-vitro. Additionally, the CellDrum enables to elucidate the mechanical response of cells to pharmacological drugs, toxins and vasoactive agents. Due to its highly flexible polymer support, cells can also be mechanically stimulated by steady and cyclic biaxial stretching.
Y1  - 2019
SN  - 978-3-940402-22-6
U6  - http://dx.doi.org/10.17185/duepublico/48750
SP  - 5
EP  - 6
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  - 
TY  - JOUR
A1  - Bassam, Rasha
A1  - Hescheler, Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
A1  - Digel, Ilya
T1  - Effects of spermine NONOate and ATP on the thermal stability of hemoglobin
JF  - BMC Biophysics
N2  - Background
Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min.

Results
Major results were:
1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment,
2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and
3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature.

Conclusion
The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell.
KW  - Nitric Oxide Donor
KW  - NONOate
KW  - Circular Dichroism
KW  - Nitric Oxide
Y1  - 2012
U6  - http://dx.doi.org/10.1186/2046-1682-5-16
SN  - 2046-1682
VL  - 5
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Bassam, Rasha
A1  - Digel, Ilya
A1  - Hescheler, Jürgen
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
T1  - Effects of spermine NONOate and ATP on protein aggregation: light scattering evidences
JF  - BMC Biophysics
Y1  - 2013
U6  - http://nbn-resolving.de/urn/resolver.pl?10.1186/2046-1682-6-1
SN  - 2046-1682
SP  - 1
EP  - 14
PB  - BioMed Central
CY  - London
ER  - 
TY  - JOUR
A1  - Bassam, Rasha
A1  - Artmann, Gerhard
A1  - Hescheler, Jürgen
A1  - Graef, T.
A1  - Temiz Artmann, Aysegül
A1  - Porst, Dariusz
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Arinkin, Vladimir
A1  - Gossmann, Matthias
A1  - Digel, Ilya
T1  - Alterations in human hemoglobin structure related to red blood cell storage
N2  - The importance of the availability of stored blood or blood cells, respectively, for urgent transfusion cannot be overestimated. Nowadays, blood storage becomes even more important since blood products are used for epidemiological studies, bio-technical research or banked for transfusion purposes. Thus blood samples must not only be processed, stored, and shipped to preserve their efficacy and safety, but also all parameters of storage must be recorded and reported for Quality Assurance. Therefore, blood banks and clinical research facilities are seeking more accurate, automated means for blood storage and blood processing.
KW  - Hämoglobin
KW  - Hämoglobinstruktur
KW  - Blutzellenlagerung
KW  - Hemoglobin structure
KW  - Red blood cell storage
Y1  - 2011
ER  - 
TY  - BOOK
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
A1  - Zhubanova, Azhar A.
A1  - Digel, Ilya
ED  - Artmann, Gerhard
ED  - Temiz Artmann, Aysegül
ED  - Zhubanova, Azhar A.
ED  - Digel, Ilya
T1  - Biological, physical and technical basics of cell engineering
Y1  - 2018
SN  - 978-981-10-7903-0
PB  - Springer
CY  - Singapore
ER  - 
TY  - CHAP
A1  - Artmann, Gerhard
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Zaccai, Giuseppe
A1  - Büldt, Georg
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - The crucial role of water in a phase transition of hemoglobin at body temperature : [abstract]
N2  - The observation of a temperature transition of hemoglobin occurring at a critical temperature close to body temperature
KW  - Hämoglobin
KW  - Erythrozyt
KW  - Körpertemperatur
Y1  - 2010
ER  - 
TY  - CHAP
A1  - Artmann, Gerhard
A1  - Meruvu, Haritha
A1  - Kizildag, Sefa
A1  - Temiz Artmann, Aysegül
ED  - Artmann, Gerhard
ED  - Temiz Artmann, Aysegül
ED  - Zhubanova, Azhar A.
ED  - Digel, Ilya
T1  - Functional Toxicology and Pharmacology Test of Cell Induced Mechanical Tensile Stress in 2D and 3D Tissue Cultures
T2  - Biological, Physical and Technical Basics of Cell Engineering
N2  - Mechanical forces/tensile stresses are critical determinants of cellular growth, differentiation and migration patterns in health and disease. The innovative “CellDrum technology” was designed for measuring mechanical tensile stress of cultured cell monolayers/thin tissue constructs routinely. These are cultivated on very thin silicone membranes in the so-called CellDrum. The cell layers adhere firmly to the membrane and thus transmit the cell forces generated. A CellDrum consists of a cylinder which is sealed from below with a 4 μm thick, biocompatible, functionalized silicone membrane. The weight of cell culture medium bulbs the membrane out downwards. Membrane indentation is measured. When cells contract due to drug action, membrane, cells and medium are lifted upwards. The induced indentation changes allow for lateral drug induced mechanical tension quantification of the micro-tissues. With hiPS-induced (human) Cardiomyocytes (CM) the CellDrum opens new perspectives of individualized cardiac drug testing. Here, monolayers of self-beating hiPS-CMs were grown in CellDrums. Rhythmic contractions of the hiPS-cells induce membrane up-and-down deflections. The recorded cycles allow for single beat amplitude, single beat duration, integration of the single beat amplitude over the beat time and frequency analysis. Dose effects of agonists and antagonists acting on Ca2+ channels were sensitively and highly reproducibly observed. Data were consistent with published reference data as far as they were available. The combination of the CellDrum technology with hiPS-Cardiomyocytes offers a fast, facile and precise system for pharmacological and toxicological studies. It allows new preclinical basic as well as applied research in pharmacolgy and toxicology.
Y1  - 2018
SN  - 978-981-10-7904-7
U6  - http://dx.doi.org/10.1007/978-981-10-7904-7_7
SP  - 157
EP  - 192
PB  - Springer
CY  - Singapore
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Zerlin, Kay
A1  - Maggakis-Kelemen, Christina
A1  - Linder, Peter
A1  - Porst, Dariusz
A1  - Kayser, Peter
A1  - Stadler, David
A1  - Dikta, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Hemoglobin senses body temperature
JF  - European Biophysics Journal
Y1  - 2009
SN  - 0175-7571
VL  - 38
IS  - 5
SP  - 589
EP  - 600
ER  - 
TY  - CHAP
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Linder, Peter
A1  - Temiz Artmann, Aysegül
T1  - Biophysical and Engineering Contributions to Plant Research
N2  - Tests with palm tree leaves have just started yet and scan data are in the process to be analyzed. The final goal of future project for palm tree gender and species recognition will be to develop optical scanning technology to be applied to date palm tree leaves for in–situ screening purposes. Depending on the software used and the particular requirements of the users the technology potentially shall be able to identify palm tree diseases, palm tree gender, and species of young date palm trees by scanning leaves.
KW  - Pflanzenphysiologie
KW  - Dattel
KW  - Pflanzenscanner
KW  - plant scanner
KW  - date palm tree
Y1  - 2011
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Burns, Laura
A1  - Canaves, Jaume M.
A1  - Temiz Artmann, Aysegül
T1  - Circular dichroism spectra of human hemoglobin reveal a reversible structural transition at body temperature
JF  - European Biophysics Journal. 33 (2004), H. 6
Y1  - 2004
SN  - 1432-1017
SP  - 490
EP  - 496
ER  - 
TY  - JOUR
A1  - Arinkin, Vladimir
A1  - Digel, Ilya
A1  - Porst, Dariusz
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
T1  - Phenotyping date palm varieties via leaflet cross-sectional imaging and artificial neural network application
JF  - BMC bioinformatics
N2  - Background
True date palms (Phoenix dactylifera L.) are impressive trees and have served as an indispensable source of food for mankind in tropical and subtropical countries for centuries. The aim of this study is to differentiate date palm tree varieties by analysing leaflet cross sections with technical/optical methods and artificial neural networks (ANN).

Results
Fluorescence microscopy images of leaflet cross sections have been taken from a set of five date palm tree cultivars (Hewlat al Jouf, Khlas, Nabot Soltan, Shishi, Um Raheem). After features extraction from images, the obtained data have been fed in a multilayer perceptron ANN with backpropagation learning algorithm.

Conclusions
Overall, an accurate result in prediction and differentiation of date palm tree cultivars was achieved with average prediction in tenfold cross-validation is 89.1% and reached 100% in one of the best ANN.
Y1  - 2014
U6  - http://dx.doi.org/10.1186/1471-2105-15-55
SN  - 1471-2105
VL  - 15
IS  - 55
SP  - 1
EP  - 8
ER  - 
TY  - JOUR
A1  - Amin, Rashid
A1  - Temiz Artmann, Aysegül
A1  - Artmann, Gerhard
A1  - Lazarovici, Philip
A1  - Lelkes, Peter I.
T1  - Permeability of an In Vitro Model of Blood Brain Barrier (BBB)
JF  - 13th International Conference on Biomedical Engineering / Lim, Chwee Teck [Ed.]
Y1  - 2009
SN  - 978-3-540-92841-6
N1  - IFMBE Proceedings ; 23, Track 1 ; ICBME 2008 3–6 December 2008 Singapore
SP  - 81
EP  - 84
ER  - 
TY  - JOUR
A1  - Akimbekov, Nuraly Shardarbekovich
A1  - Mansurov, Zulkhair
A1  - Jandosov, J.
A1  - Digel, Ilya
A1  - Gossmann, Matthias
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
A1  - Zhubanova, Azhar A.
T1  - Wound healing activity of carbonized rice husk
N2  - The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls.
KW  - Wundheilung
KW  - Epithel
KW  - Fibroblast
KW  - carbonized rice husk
KW  - wound healing
KW  - epithelization
KW  - human dermal fibroblasts
Y1  - 2013
PB  - Trans Tech Publications, Switzerland
CY  - Bäch
ER  - 
TY  - JOUR
A1  - Akimbekov, N. Sh.
A1  - Zhubanova, A. A.
A1  - Mansurov, Z. A.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Use of Carbonized Rise Shell for the local treatment of wounds
JF  - Eurasian ChemTech Journal
N2  - On the model of musculocutaneous wound in rats, the effect of applicative sorption by carbonized rise shell (CRS) on the healing of festering wound was studied. It has been shown, that cytological changes end with rapid scar formation. The use of CRS at the period of severe purulent wound contributes to its favorable course, prevents the development of complications of the animals from sepsis.
Y1  - 2010
U6  - http://dx.doi.org/10.18321/ectj35
SN  - 2522-4867
VL  - 12
IS  - 2
SP  - 133
EP  - 138
PB  - Institute of Combustion Problems
CY  - Almaty
ER  -