TY - JOUR A1 - Yoshinobu, T. A1 - Iwasaki, H. A1 - Ui, Y. A1 - Furuichi, K. A1 - Ermelenko, Y. A1 - Mourzina, Y. A1 - Wagner, Torsten A1 - Näther, Niko A1 - Schöning, Michael Josef T1 - The light-addressable potentiometric sensor for multi-ion sensing and imaging JF - Methods. 37 (2005), H. 1 Y1 - 2005 SN - 1046-2023 SP - 99 EP - 102 ER - TY - JOUR A1 - Yoshinobu, T. A1 - Ermelenko, Y. A1 - Mourzina, Y. A1 - Schöning, Michael Josef A1 - Iwasaki, H. A1 - Vlasov, Y. T1 - Multi-component analysis based on the light-addressable potentiometric sensor (LAPS) JF - Proceedings : 11th international conference Sensor 2003; 13 - 15 May 2003, Exhibition Centre Nuremberg, Germany Y1 - 2003 N1 - Internationale Messe mit Kongress Sensor <11, 2003, Nürnberg> SP - 141 EP - 144 PB - AMA Service CY - Wunstorf ER - TY - JOUR A1 - Yoshinobu, T. A1 - Ecken, H. A1 - Poghossian, Arshak A1 - Simonis, A. A1 - Iwasaki, H. A1 - Lüth, H. A1 - Schöning, Michael Josef T1 - Constant-current-mode LAPS (CLAPS) for the detection of penicillin JF - Electroanalysis. 13 (2001), H. 8-9 Y1 - 2001 SN - 1040-0397 SP - 733 EP - 736 ER - TY - JOUR A1 - Yoshinobu, T. A1 - Ecken, H. A1 - Poghossian, Arshak A1 - Lüth, H. A1 - Iwasaki, H. A1 - Schöning, Michael Josef T1 - Alternative sensor materials for light-addressable potentiometric sensors JF - Sensors and Actuators B. 76 (2001), H. 1-3 Y1 - 2001 SN - 0925-4005 SP - 388 EP - 392 ER - TY - JOUR A1 - Yoshinobu, T. A1 - Ecken, H. A1 - Ismail, Md.A.B. A1 - Iwasaki, H. A1 - Lüth, H. A1 - Schöning, Michael Josef T1 - Chemical imaging sensor and its application to biological systems JF - Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz Y1 - 2001 SN - 0-08-044014-2 SP - 259 EP - 263 PB - Elsevier [u.a.] CY - Amsterdam [u.a.] ER - TY - JOUR A1 - Yazdanbakhsh, Pedram A1 - Solbach, Klaus A1 - Bitz, Andreas T1 - Variable power combiner for RF mode shimming in 7-T MR imaging JF - IEEE Transaction on Biomedical Engineering N2 - This contribution discusses the utilization of RF power in an MRI system with RF mode shimming which enables the superposition of circularly polarized modes of a transmit RF coil array driven by a Butler matrix. Since the required power for the individual modes can vary widely, mode-shimming can result in a significant underutilization of the total available RF power. A variable power combiner (VPC) is proposed to improve the power utilization: it can be realized as a reconfiguration of the MRI transmit system by the inclusion of one additional matrix network which receives the power from all transmit amplifiers at its input ports and provides any desired (combined) power distribution at its output ports by controlling the phase and amplitude of the amplifiers’ input signals. The power distribution at the output ports of the VPC is then fed into the “mode” ports of the coil array Butler matrix in order to superimpose the spatial modes at the highest achievable power utilization. The VPC configuration is compared to the standard configuration of the transmit chain of our MRI system with 8 transmit channels and 16 coils. In realistic scenarios, improved power utilization was achieved from 17% to 60% and from 14% to 55% for an elliptical phantom and a region of interest in the abdomen, respectively, and an increase of the power utilization of 1 dB for a region of interest in the upper leg. In general, it is found that the VPC allows significant improvement in power utilization when the shimming solution demands only a few modes to be energized, while the technique can yield loss in power utilization in cases with many modes required at high power level. Y1 - 2012 U6 - http://dx.doi.org/10.1109/TBME.2012.2205926 SN - 1558-2531 VL - 59 IS - 9 SP - 2549 EP - 2557 PB - IEEE CY - New York ER - TY - JOUR A1 - Yang, Peng-Fei A1 - Kriechbaumer, Andreas A1 - Albracht, Kirsten A1 - Sanno, Maximilian A1 - Ganse, Bergita A1 - Koy, Timmo A1 - Shang, Peng A1 - Brüggemann, Gert-Peter A1 - Müller, Lars Peter A1 - Rittweger, Jörn T1 - On the relationship between tibia torsional deformation and regional muscle contractions in habitual human exercises in vivo JF - Journal of Biomechanics Y1 - 2015 U6 - http://dx.doi.org/10.1016/j.jbiomech.2014.12.031 SN - 0021-9290 VL - 48 IS - 3 SP - 456 EP - 464 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Yang, Peng-Fei A1 - Kriechbaumer, Andreas A1 - Albracht, Kirsten A1 - Sanno, Maximilian A1 - Ganse, Bergita A1 - Koy, Timmo A1 - Shang, Peng A1 - brüggemann, Gert-Peter A1 - Müller, Lars Peter A1 - Rittweger, Jörn T1 - A novel optical approach for assessing in vivo bone segment deformation and its application in muscle-bone relationship studies in humans JF - Journal of Orthopaedic Translation Y1 - 2014 U6 - http://dx.doi.org/10.1016/j.jot.2014.07.078 SN - 2214-0328 SN - 2214-031X VL - 2 IS - 4 SP - 238 EP - 238 PB - Elsevier CY - Singapore ER - TY - CHAP A1 - Xu, Zhanping A1 - Schwarte, Rudolf A1 - Heinol, Horst-Guenther A1 - Buxbaum, Bernd A1 - Ringbeck, Thorsten ED - Cheung, Edmund H. M. T1 - Smart pixel : photonic mixer device (PMD) ; new system concept of a 3D-imaging camera-on-a-chip T2 - Proceedings : M2VIP '98; Nanjing, China, 10th - 12th September 1998 Y1 - 1998 SN - 962-442-129-3 N1 - International Conference on Mechatronics and Machine Vision in Practice <5, 1998, Nanking> ; M2VIP <5, 1998, Nanking> SP - 259 EP - 264 CY - Hong Kong ER - TY - JOUR A1 - Xu, Jung-Ming A1 - Lü, Min A1 - Ma, Meijie A1 - Hellwig, Angelika T1 - Super connectivity of line graphs JF - Information processing letters Y1 - 2005 SN - 1872-6119 (E-Journal); 0020-0190 (Print) VL - Vol. 94 IS - Iss. 4 SP - 191 EP - 195 ER - TY - JOUR A1 - Wulfhorst, Helene A1 - Duwe, Anna-Maria A1 - Merseburg, Johannes A1 - Tippkötter, Nils T1 - Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis JF - Tetrahedron N2 - The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.tet.2016.04.029 VL - 72 IS - 46 SP - 7329 EP - 7334 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Wulfhorst, H. A1 - Duwe, A. A1 - Möhring, S. A1 - Jurca, O. A1 - Tippkötter, Nils T1 - Analysis of pretreated biomass by differential scanning 132 calorimetry and multivariate data analysis T2 - New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany Y1 - 2016 SP - 132 PB - DECHEMA CY - Frankfurt am Main ER - TY - CHAP A1 - Wu, Ziyi A1 - Kemper, Hans T1 - The optimal 48 V – battery pack for a specific load profile of a heavy duty vehicle T2 - 8. Internationale Fachtagung Kraftwerk Batterie : 26. – 27. April 2016, Münster, Deutschland Y1 - 2016 ER - TY - CHAP A1 - Wu, Chunsheng A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Bronder, Thomas A1 - Bäcker, Matthias A1 - Wang, Ping A1 - Schöning, Michael Josef T1 - An application of a scanning light-addressable potentiometric sensor for label-free DNA detection T2 - 11. Dresdner Sensor-Symposium : 9.-11.12.2013 Y1 - 2013 SN - 978-3-9813484-5-3 SP - 164 EP - 168 ER - TY - JOUR A1 - Wu, Chunsheng A1 - Poghossian, Arshak A1 - Bronder, Thomas A1 - Schöning, Michael Josef T1 - Sensing of double-stranded DNA molecules by their intrinsic molecular charge using the light-addressable potentiometric sensor JF - Sensors and Actuators B: Chemical N2 - A multi-spot light-addressable potentiometric sensor (LAPS), which belongs to the family of semiconductor field-effect devices, was applied for label-free detection of double-stranded deoxyribonucleic acid (dsDNA) molecules by their intrinsic molecular charge. To reduce the distance between the DNA charge and sensor surface and thus, to enhance the electrostatic coupling between the dsDNA molecules and the LAPS, the negatively charged dsDNA molecules were electrostatically adsorbed onto the gate surface of the LAPS covered with a positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)). The surface potential changes in each spot of the LAPS, induced by the layer-by-layer adsorption of a PAH/dsDNA bilayer, were recorded by means of photocurrent-voltage and constant-photocurrent measurements. In addition, the surface morphology of the gate surface before and after consecutive electrostatic adsorption of PAH and dsDNA layers was studied by atomic force microscopy measurements. Moreover, fluorescence microscopy was used to verify the successful adsorption of dsDNA molecules onto the PAH-modified LAPS surface. A high sensor signal of 25 mV was registered after adsorption of 10 nM dsDNA molecules. The lower detection limit is down to 0.1 nM dsDNA. The obtained results demonstrate that the PAH-modified LAPS device provides a convenient and rapid platform for the direct label-free electrical detection of in-solution hybridized dsDNA molecules. KW - Layer-by-layer adsorption KW - Poly(allylamine hydrochloride) KW - Label-free detection KW - DNA biosensor KW - LAPS KW - Field effect Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.snb.2016.02.004 SN - 0925-4005 IS - 229 SP - 506 EP - 512 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Schöning, Michael Josef T1 - Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer JF - Nanoscale N2 - A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent–voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout. Y1 - 2015 U6 - http://dx.doi.org/10.1039/C4NR07225A VL - 14 IS - 7 SP - 6143 EP - 6150 PB - Royal Society of Chemistry (RSC) CY - Cambridge ER - TY - JOUR A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Werner, Frederik A1 - Bäcker, Matthias A1 - Schöning, Michael Josef T1 - Label-free electrical detection of DNA with a multi-spot LAPS: First step towards light-addressable DNA chips JF - Physica status solidi A : Applications and materials science N2 - A multi-spot (4 × 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent–voltage (I–V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I–V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift (∼5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge. Y1 - 2014 U6 - http://dx.doi.org/10.1002/pssa.201330442 SN - 1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) VL - 211 IS - 6 SP - 1423 EP - 1428 PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Wu, Chunsheng A1 - Bronder, Thomas A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer T2 - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250) Y1 - 2014 SN - 978-3-8007-3622-5 SP - 1 EP - 4 PB - VDE-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Wrede, Oliver T1 - Mnemotechnik in graphischen Benutzeroberflächen JF - Form / Form-Diskurs : Zeitschrift für Design und Theorie. 1 (1996), H. 2 Y1 - 1996 ER - TY - JOUR A1 - Wrede, Oliver T1 - Continuity in travel information JF - Information Design Journal N2 - This article discusses the contrast between the information transportation companies provide to travellers and that of their brand messaging. Companies’ brand messaging often portrays the service they provide as pleasant, stress free and perfect. Customers and users of the service, on the other hand, often describe their experience of the service as a negative one. This article suggests that the brand value would be greater if transportation companies paid more attention to the users’ experience when designing their information systems, particularly in worst case scenarios. Y1 - 2016 U6 - http://dx.doi.org/10.1075/idj.22.2.09wre SN - 0142-5471 N1 - Gedruckt vorhanden in der Bereichsbibliothek Boxgraben unter 53 Z 971-2016 (Magazin) VL - 22 IS - 2 SP - 172 EP - 178 PB - John Benjamins CY - Amsterdam ER - TY - BOOK A1 - Wosnitza, Franz A1 - Hilgers, Hans Gerd T1 - Energieeffizienz und Energiemanagement : ein Überblick heutiger Möglichkeiten und Notwendigkeiten Y1 - 2012 SN - 978-3-8348-1941-3 N1 - eISBN 978-3-8348-8671-2 PB - Springer Spektrum CY - Wiesbaden ER - TY - CHAP A1 - Wollert, Jörg A1 - Plessmann, K. W. T1 - Controlling processes by operating-point based hybride observers T2 - EUFIT '93 : September 7 - 10, 1993, Eurogress Aachen, Germany, proceedings / First European Congress on Fuzzy and Intelligent Technologies Y1 - 1993 SN - 3-86073-176-9 SP - 1151 EP - 1155 PB - Verl. d. Augustinus Buchh. CY - Aachen ER - TY - CHAP A1 - Wollert, Jörg T1 - The performance of UWB-communication in an industrial environment T2 - IEEE 1st International Symposium on Wireless Systems : (IDAACS-SWS) : Offenburg, 20-21 Sept. 2012 Y1 - 2012 SN - 978-1-4673-4678-8 U6 - http://dx.doi.org/10.1109/IDAACS-SWS.2012.6377639 SP - 85 EP - 90 PB - IEEE CY - Piscataway, NJ ER - TY - CHAP A1 - Wollert, Jörg T1 - Wireless systems for machinery safety : Requirements and solutions for wireless real time systems T2 - 16th International Conference on Research and Education in Mechatronics (REM), 2015 Y1 - 2015 SN - 978-3-945728-01-7 U6 - http://dx.doi.org/10.1109/REM.2015.7380377 SP - 88 EP - 91 ER - TY - JOUR A1 - Wollbrink, Moritz A1 - Maslo, Semir A1 - Zimmer, Daniel A1 - Abbas, Karim A1 - Arntz, Kristian A1 - Bergs, Thomas T1 - Clamping and substrate plate system for continuous additive build-up and post-processing of metal parts JF - Procedia CIRP N2 - The manufacturing share of laser powder bed fusion (L-PBF) increases in industrial application, but still many process steps are manually operated. Additionally, it is not possible to achieve tight dimensional tolerances or low surfaces roughness. Hence, a process chain has to be set up to combine additive manufacturing (AM) with further machining technologies. To achieve a continuous workpiece flow as basis for further industrialization of L-PBF, the paper presents a novel substrate system and its application on L-PBF machines and post-processing. The substrate system consists of a zero-point clamping system and a matrix-like interface of contact pins to be substantially connected to the workpiece within the L-PBF process. Y1 - 2020 U6 - http://dx.doi.org/10.1016/j.procir.2020.04.015 SN - 2212-8271 VL - 93 SP - 108 EP - 113 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wolisz, Henryk A1 - Schütz, Thomas A1 - Blanke, Tobias A1 - Hagenkamp, Markus A1 - Kohrn, Markus A1 - Wesseling, Mark A1 - Müller, Dirk T1 - Cost optimal sizing of smart buildings' energy system components considering changing end-consumer electricity markets JF - Energy Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.energy.2017.06.025 VL - 137 SP - 715 EP - 728 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Wolff, Nino A1 - Seefeldt, Patric A1 - Bauer, Wolfgang A1 - Fiebig, Christopher A1 - Gerding, Patrick A1 - Parow-Souchon, Kai A1 - Pongs, Anna A1 - Reiffenrath, Matti A1 - Ziemann, Thomas T1 - Alternative application of solar sail technology T2 - Advances in solar sailing N2 - The development of Gossamer sail structures for solar sails contributes to a large field of future space applications like thin film solar generators, membrane antennas and drag sails. The focus of this paper is the development of a drag sail based on solar sail technology that could contribute to a reduction of space debris in low Earth orbits. The drag sail design and its connections to solar sail development, a first test on a sounding rocket, as well as the ongoing integration of the drag sail into a triple CubeSat is presented. Y1 - 2014 SN - 978-3-642-34906-5 (Print) ; 978-3-642-34907-2 (E-Book) U6 - http://dx.doi.org/10.1007/978-3-642-34907-2_23 SP - 351 EP - 365 PB - Springer CY - Berlin ER - TY - CHAP A1 - Wolf, Martin A1 - Wiese, Ute T1 - A comparative transformation model for process changes using serious games T2 - 2013 IEEE 2nd International Conference on Serious Games and Applications for Health (SeGAH): proceedings of a meeting held 2-3 May 2013, Algarve, Portugal Y1 - 2013 SN - 978-1-4673-6164-4 U6 - http://dx.doi.org/10.1109/SeGAH.2013.6665307 SP - 64 EP - 70 PB - Institute of Electrical and Electronics Engineers (IEEE) CY - Piscataway, NJ ER - TY - JOUR A1 - Wolf, Martin A1 - Simon, S. A1 - Schlick, Christopher T1 - Qualitative and Quantitative Analysis of Cooperation / Wolf, M. ; Simon, S. ; Schlick, C. JF - Proceedings : May 26 - 29, 1998, Cannes (France) = Actes : 26 - 29 mai 1998 / INRIA, Institut National de Recherche en Informatique et en Automatique, Unité de recherche ... F. Darses ..., eds Y1 - 1998 N1 - COOP ; Institut National de Recherche en Informatique et en Automatique ; Centre ; (Sophia Antipolis) ; International Conference on the Design of Cooperative Systems ; (3 ; 1998.05.26-29 ; Cannes) ; Conference Internationale sur la Conception de Systèmes Coopératifs ; (3 ; 1998.05.26-29 ; Cannes) ; COOP ; (3 ; 1998.05.26-29 ; Cannes) SP - 1 EP - 5 PB - - CY - Cannes ER - TY - JOUR A1 - Wolf, Martin A1 - Schlick, Christopher A1 - Springer, J. ED - Miyamoto, Hiroyuki T1 - The use of implicit communication mechanisms in desktop-teleconferencing-systems JF - Proceedings of WWDU '97 Tokyo, Fifth International Scientific Conference on Work with Display Units : November 3 - 5, 1997, Tokyo Y1 - 1997 N1 - International Scientific Conference on Work with Display Units ; International Scientific Conference on Work with Display Units ; (5 ; 1997.11.03-05 ; Tokyo) SP - 193 EP - 194 PB - - CY - Tokyo ER - TY - JOUR A1 - Wolf, Martin A1 - Luczak, Holger A1 - Springer, J. A1 - Simon, S. ED - Miyamoto, Hiroyuki T1 - The role of metaphors in computer supported cooperative work JF - Proceedings of WWDU '97 Tokyo, Fifth International Scientific Conference on Work with Display Units : November 3 - 5, 1997, Tokyo / Y1 - 1997 N1 - International Scientific Conference on Work with Display Units ; International Scientific Conference on Work with Display Units ; (5 ; 1997.11.03-05 ; Tokyo) SP - 191 EP - 192 PB - - CY - Tokyo ER - TY - JOUR A1 - Wolf, Martin A1 - Luczak, Holger T1 - Computer Supported Communication and Cooperation – Making Information Aware / Luczak, H. ; Wolf, M. JF - Human-computer interaction : proceedings of HCI International '99 (the 8th International Conference on Human-Computer Interaction), Munich, Germany, August 22 - 26, 1999 Y1 - 1999 N1 - HCI - International Conference on Human-Computer Interaction ; 8 (Munich, Germany) : 1999.08.22-26 ; in 2 Bänden SP - 298 EP - 302 PB - Erlbaum CY - Mahwah, NJ ER - TY - CHAP A1 - Wolf, Martin A1 - Lenz, Laura L. T1 - The economic effectiveness of serious games in the healthcare environment : application and evaluation of the Comparative Transformation Model (CTM) T2 - IEEE 3rd International Conference on Serious Games and Applications for Health : SeGAH 2014 ; Rio de Janeiro, Brazil, May 14 - 16 Y1 - 2015 SN - 978-1-4799-4823-9 U6 - http://dx.doi.org/10.1109/SeGAH.2014.7067089 SP - 135 EP - 142 PB - IEEE [u.a.] CY - Piscataway, NJ ER - TY - JOUR A1 - Wolf, Martin A1 - Foltz, Christian A1 - Schlick, Christopher A1 - Luczak, Holger T1 - Empirical Investigation of a workspace model for Chemical engineers / Wolf, Martin ; Foltz, Christian ; Schlick, Christopher ; Luczak, Holger JF - Proceedings of the Human Factors and Ergonomics Society Annual Meeting July 2000. 44 (2000), H. 6 Y1 - 2000 N1 - Proceedings of the IEA 2000/HFES 2000 Congress SP - 612 EP - 615 PB - - ER - TY - JOUR A1 - Wolf, Martin A1 - Foltz, Christian A1 - Schlick, Christopher A1 - Luczak, Holger T1 - Development and evaluation of a groupware system to support chemical design processes / Wolf, Martin ; Foltz, Christian ; Schlick, Christopher ; Luczak, Holger JF - International Journal of Human-Computer Interaction . 14 (2002), H. 2 Y1 - 2002 SN - 1044-7318 SP - 181 EP - 198 PB - - ER - TY - JOUR A1 - Wolf, Martin A1 - Foltz, Christian A1 - Schlick, Christopher A1 - Luczak, Holger T1 - Groupware support for chemical process design / Wolf, M.; Foltz, C.; Schlick, C.; Luczak, H. JF - Human-computer interaction : proceedings of HCI International '99 (the 8th International Conference on Human-Computer Interaction), Munich, Germany, August 22 - 26, 1999 Y1 - 1999 N1 - HCI - International Conference on Human-Computer Interaction ; 8 (Munich, Germany) : 1999.08.22-26 ; in 2 Bänden SP - 343 EP - 347 PB - Erlbaum CY - Mahwah, NJ ER - TY - JOUR A1 - Wolf, Martin A1 - Foltz, Christian A1 - Killich, Stephan A1 - Schmidt, Ludger T1 - Task and Information Modeling for Cooperative Work / Foltz, Christian ; Killich, Stephan ; Wolf, Martin ; Schmidt, Ludger ; Luczak, Holger JF - Systems, social and internationalization design aspects of human-computer interaction / ed. by Michael J. Smith, Gavriel Salvendy Vol. 2 Y1 - 2001 SN - 0-8058-3608-X N1 - International Conference on Human Computer Interaction 9, 2001, New Orleans, La. SP - 172 EP - 176 PB - - ER - TY - BOOK A1 - Wolf, Martin A1 - Foltz, Christian A1 - Killich, S. T1 - K3 User Guide Y1 - 2000 SP - 1 EP - 13 PB - RWTH CY - Aachen ER - TY - JOUR A1 - Wolf, Martin T1 - Groupware related task design JF - ACM SIGGROUP Bulletin. 21 (2000), H. 2 Y1 - 2000 SP - 5 EP - 8 PB - - ER - TY - JOUR A1 - Wolf, Martin T1 - Groupware related task design JF - ACM SIGGROUP Bulletin N2 - his report summarizes the results of a workshop on Groupware related task design which took place at the International Conference on Supporting Group Work Group'99, Arizona, from 14 th to 17 th November 1999. The workshop was addressed to people from different viewpoints, backgrounds, and domains: - Researchers dealing with questions of task analysis and task modeling for Groupware application from an academic point of view. They may contribute modelbased design approaches or theoretically oriented work - Practitioners with experience in the design and everyday use of groupware systems. They might refer to the practical side of the topic: "real" tasks, "real" problems, "real" users, etc. Y1 - 2000 U6 - http://dx.doi.org/10.1145/605660.605662 SN - 2372-7403 VL - 21 IS - 2 SP - 5 EP - 8 ER - TY - CHAP A1 - Wolf, C. Roland A1 - Kapelyukh, Yury A1 - Scheer, Nico A1 - Henderson, Colin J. ED - Wilson, Alan G. E. T1 - Application of Humanised and Other Transgenic Models to Predict Human Responses to Drugs N2 - The use of transgenic animal models has transformed our knowledge of complex biochemical pathways in vivo. It has allowed disease processes to be modelled and used in the development of new disease prevention and treatment strategies. They can also be used to define cell- and tissue-specific pathways of gene regulation. A further major application is in the area of preclinical development where such models can be used to define pathways of chemical toxicity, and the pathways that regulate drug disposition. One major application of this approach is the humanisation of mice for the proteins that control drug metabolism and disposition. Such models can have numerous applications in the development of drugs and in their more sophisticated use in the clinic. Y1 - 2015 SN - 978-1-78262-778-4 U6 - http://dx.doi.org/10.1039/9781782622376-00152 SP - 152 EP - 176 PB - RSC Publ. CY - Cambridge ER - TY - JOUR A1 - Wittmann, Klaus A1 - Ulamec, S. A1 - Feuerbacher, B. A1 - Rosenbauer, H. (u.a.) T1 - Rosetta Lander - In Situ Investigation of a Cometary Nucleus JF - Lunar and Planetary Science. 28 (1997) Y1 - 1997 SN - 0270-9511 N1 - Proceedings of the 28th Annual Lunar and Planetary Science Conference, March 17-21, 1997, Houston, Texas SP - 1461 EP - 1462 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Ulamec, S. A1 - Feuerbacher, B. A1 - Rosenbauer, H. T1 - Technical Challenge, Scientific Thrill: A Long Term Lander on an Active Comet JF - Lunar and Planetary Science. 26 (1995) Y1 - 1995 SN - 0270-9511 N1 - Proceedings of the 26th Lunar and Planetary Science Conference / org. by Lunar and Planetary Institute, Houston Texas SP - 1431 EP - 1432 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Ulamec, S A1 - Richter, L T1 - Lander Systems for Planetary Missions JF - Laboratory astrophysics and space research / ed. by P. Ehrenfreund Y1 - 1999 SN - 0-7923-5338-2 N1 - Serie Astrophysics and space science library 236 SP - 597 EP - 621 PB - Kluwer CY - Dordrecht [u.a.] ER - TY - JOUR A1 - Wittmann, Klaus A1 - Schmidt, H. P. A1 - Padeken, D. A1 - Hildmann, B. T1 - The Microgravity User Support Centre (MUSC) JF - ESA SP. 277 (1988) Y1 - 1988 SN - 0379-6566 N1 - Proceedings of the International Symposium on Europe in Space--the Manned Space System : 25-29 April 1988, Strasbourg, France David, Valerie ; Longdon, Norman [ed] SP - 525 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Schmidt, H. P. A1 - Feuerbacher, B. T1 - Microgravity research and user support in the Space Station era: The Microgravity User Support Centre JF - Acta Astronautica. 17 (1988), H. 11-12 Y1 - 1988 SN - 0094-5765 SP - 1161 EP - 1168 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Padeken, D. T1 - The User Center for Microgravity MUSC - Support for space experiments JF - DLR-Nachrichten. 70 (1993) Y1 - 1993 SN - 0937-0420 SP - 19 EP - 24 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Kell, G. A1 - Winkler, F. A1 - Günther, M. (u.a.) T1 - High resolution temperature measurement technique for materials sciences experiments in space JF - Acta Astronautica. 43 (1998), H. 7-8 Y1 - 1998 SN - 0094-5765 SP - 385 EP - 395 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Kehr, J. A1 - Willnecker, R. T1 - Decentralized Payload Operations Concept for Columbus Y1 - 2000 N1 - 51st IAF Congress, Rio de Janeiro, Brazil, Oct. 2000 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Gillessen, F. A1 - Otto, G. A1 - Röstel, R. T1 - Undercooling and solidification of metallic melts suspended in a non-nucleating matrix: Experiment NUCAL of the German Spacelab Mission D-2 JF - Acta Astronautica. 38 (1996), H. 1 Y1 - 1996 SN - 0094-5765 SP - 63 EP - 68 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Fortezza, R. T1 - Telescience opportunities in the precursor flights JF - Space Technology - Industrial and Commercial Applications. 13 (1992), H. 2 Y1 - 1992 SN - 0892-9270 SP - 221 EP - 226 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Feuerbacher, B. A1 - Ulamec, S. A1 - Rosenbauer, H. (u.a.) T1 - Rosetta Lander - In Situ Characterisation of a Comet Nucleus JF - Acta Astronautica. 45 (1999), H. 4-9 Y1 - 1999 SN - 0094-5765 SP - 389 EP - 395 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Bibring, J.-P. A1 - Rosenbauer, H. A1 - Boehnhardt, H. T1 - The Rosetta Lander (“Philae”) Investigations / Bibring, J.-P. ; Rosenbauer, H. ; Boehnhardt, H. ; [...] Wittmann, K. JF - Space Science Reviews. 128 (2007), H. 1-4 Y1 - 2007 SN - 0038-6308 SP - 205 EP - 220 ER - TY - JOUR A1 - Wittmann, Klaus A1 - Bewersdorff, A. A1 - Görler, G.P. A1 - Otto, G. T1 - Solidification of silver-germanium alloys in an amorphous matrix aboard the space station Mir JF - Acta Astronautica. 29 (1993), H. 7 Y1 - 1993 SN - 0094-5765 SP - 547 EP - 552 ER - TY - JOUR A1 - Wittmann, Klaus T1 - The microgravity User Support Centre JF - Space Technology - Industrial and Commercial Applications. 13 (1993), H. 2 Y1 - 1993 SN - 0892-9270 SP - 121 EP - 124 ER - TY - JOUR A1 - Wittmann, Klaus T1 - Overview of Space Operations at DLR Y1 - 2000 N1 - SpaceOps2000 Conference, Toulouse, Frankreich,19-23 June 2000 ER - TY - CHAP A1 - Wittig, M. A1 - Rütters, René A1 - Bragard, Michael ED - Reiff-Stephan, Jörg ED - Jäkel, Jens ED - Schwarz, André T1 - Application of RL in control systems using the example of a rotatory inverted pendulum T2 - Tagungsband AALE 2024 : Fit für die Zukunft: praktische Lösungen für die industrielle Automation N2 - In this paper, the use of reinforcement learning (RL) in control systems is investigated using a rotatory inverted pendulum as an example. The control behavior of an RL controller is compared to that of traditional LQR and MPC controllers. This is done by evaluating their behavior under optimal conditions, their disturbance behavior, their robustness and their development process. All the investigated controllers are developed using MATLAB and the Simulink simulation environment and later deployed to a real pendulum model powered by a Raspberry Pi. The RL algorithm used is Proximal Policy Optimization (PPO). The LQR controller exhibits an easy development process, an average to good control behavior and average to good robustness. A linear MPC controller could show excellent results under optimal operating conditions. However, when subjected to disturbances or deviations from the equilibrium point, it showed poor performance and sometimes instable behavior. Employing a nonlinear MPC Controller in real time was not possible due to the high computational effort involved. The RL controller exhibits by far the most versatile and robust control behavior. When operated in the simulation environment, it achieved a high control accuracy. When employed in the real system, however, it only shows average accuracy and a significantly greater performance loss compared to the simulation than the traditional controllers. With MATLAB, it is not yet possible to directly post-train the RL controller on the Raspberry Pi, which is an obstacle to the practical application of RL in a prototyping or teaching setting. Nevertheless, RL in general proves to be a flexible and powerful control method, which is well suited for complex or nonlinear systems where traditional controllers struggle. KW - Rotatory Inverted Pendulum KW - MPC KW - LQR KW - PPO KW - Reinforcement Learning Y1 - 2024 SN - 978-3-910103-02-3 U6 - http://dx.doi.org/10.33968/2024.53 N1 - 20. AALE-Konferenz. Bielefeld, 06.03.-08.03.2024. (Tagungsband unter https://doi.org/10.33968/2024.29) SP - 241 EP - 248 PB - le-tex publishing services GmbH CY - Leipzig ER - TY - JOUR A1 - Wissenbach, U. A1 - Six, S. A1 - Bongaerts, Johannes A1 - Ternes, D. A1 - Steinwachs, S. A1 - Unden, G. T1 - A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport JF - Molecular microbiology Y1 - 1995 SN - 1365-2958 (E-Journal); 0950-382x (Print) VL - Vol. 17 IS - Iss. 4 SP - 675 EP - 686 ER - TY - JOUR A1 - Wissen, M. A1 - Bogdanski, N. A1 - Scheer, H.-C. A1 - Bitz, Andreas A1 - Ahrens, G. A1 - Gruetzner, G. T1 - Implication of the light polarisation for UV curing of pre-patterned resists JF - Microelectronic Engineering Y1 - 2005 U6 - http://dx.doi.org/10.1016/j.mee.2004.12.099 SN - 0167-9317 VL - 78-79 SP - 659 EP - 664 ER - TY - JOUR A1 - Winckler, Silvia A1 - Krueger, Rolf A1 - Schnitzler, Thomas A1 - Zang, Werner A1 - Fischer, Rainer A1 - Biselli, Manfred T1 - A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach JF - Bioprocess and biosystems engineering N2 - Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen. Y1 - 2014 U6 - http://dx.doi.org/10.1007/s00449-013-1062-8 SN - 1615-7591 (Print) 1615-7605 (Online) VL - 37 IS - 5 SP - 901 EP - 912 PB - Springer CY - Berlin, Heidelberg ER - TY - JOUR A1 - Wilson, Thomas L A1 - Blome, Hans-Joachim A1 - LaFave, Norman T1 - Astrophysical Cosmology Using a Lunar Ligo JF - Engineering, construction, and operations in space V : proceedings of the Fifth International Conference on Space '96, Albuquerque, New Mexico, June 1-6, 1996 / sponsored by Aerospace Division of the American Society of Civil Engineers ... [et al.]; edite Y1 - 1996 SN - 0-7844-0177-2 SP - 861 EP - 863 PB - The Society CY - New York ER - TY - JOUR A1 - Wilson, T. L. A1 - Blome, Hans-Joachim T1 - The Pioneer anomaly and a rotating Gödel universe JF - Advances in Space Research Y1 - 2009 SN - 0273-1177 VL - 44 IS - 11 SP - 1345 EP - 1353 ER - TY - JOUR A1 - Wilson, Ian D. A1 - Wilson, Claire E. A1 - Scheer, Nico A1 - Dickie, A.P. A1 - Schreiter, K. A1 - Wilson, E. M. A1 - Riley, R. J. A1 - Wehr, R. A1 - Bial, J. T1 - The Pharmacokinetics and Metabolism of Lumiracoxib in Chimeric Humanized and Murinized FRG Mice JF - Biochemical pharmacology Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.bcp.2017.03.015 SN - 1873-2968 VL - Volume 135 SP - 139 EP - 150 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wilson, C. E. A1 - Dickie, A. P. A1 - Schreiter, K. A1 - Wehr, R. A1 - Wilson, E. M. A1 - Bial, J. A1 - Scheer, Nico A1 - Wilson, I. D. A1 - Riley, R. J. T1 - The pharmacokinetics and metabolism of diclofenac in chimeric humanized and murinized FRG mice JF - Archives of Toxicology N2 - The pharmacokinetics of diclofenac were investigated following single oral doses of 10 mg/kg to chimeric liver humanized and murinized FRG and C57BL/6 mice. In addition, the metabolism and excretion were investigated in chimeric liver humanized and murinized FRG mice. Diclofenac reached maximum blood concentrations of 2.43 ± 0.9 µg/mL (n = 3) at 0.25 h post-dose with an AUCinf of 3.67 µg h/mL and an effective half-life of 0.86 h (n = 2). In the murinized animals, maximum blood concentrations were determined as 3.86 ± 2.31 µg/mL at 0.25 h post-dose with an AUCinf of 4.94 ± 2.93 µg h/mL and a half-life of 0.52 ± 0.03 h (n = 3). In C57BL/6J mice, mean peak blood concentrations of 2.31 ± 0.53 µg/mL were seen 0.25 h post-dose with a mean AUCinf of 2.10 ± 0.49 µg h/mL and a half-life of 0.51 ± 0.49 h (n = 3). Analysis of blood indicated only trace quantities of drug-related material in chimeric humanized and murinized FRG mice. Metabolic profiling of urine, bile and faecal extracts revealed a complex pattern of metabolites for both humanized and murinized animals with, in addition to unchanged parent drug, a variety of hydroxylated and conjugated metabolites detected. The profiles in humanized mice were different to those of both murinized and wild-type animals, e.g., a higher proportion of the dose was detected in the form of acyl glucuronide metabolites and much reduced amounts as taurine conjugates. Comparison of the metabolic profiles obtained from the present study with previously published data from C57BL/6J mice and humans revealed a greater, though not complete, match between chimeric humanized mice and humans, such that the liver humanized FRG model may represent a model for assessing the biotransformation of such compounds in humans. Y1 - 2018 U6 - http://dx.doi.org/10.1007/s00204-018-2212-1 SN - 1432-0738 VL - 92 IS - 6 SP - 1953 EP - 1967 PB - Springer ER - TY - JOUR A1 - Wilming, Anja A1 - Begemann, Jens A1 - Kuhne, Stefan A1 - Regestein, Lars A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Maurer, Karl-Heinz A1 - Büchs, Jochen T1 - Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations JF - Biochemical engineering journal Y1 - 2013 SN - 1873-295X (E-Journal); 1369-703X (Print) VL - Vol. 73 SP - 29 EP - 37 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Wilke, Thomas ED - Merlotti, Andrea T1 - Planning Process of the Di Castellamonte’s Chapel of the Holy Shroud T2 - Carlo e Amedeo di Castellamonte : 1571-1683, ingegneri e architetti per i duchi di Savoia Y1 - 2016 SN - 978-88-98229-57-4 N1 - Architettura e potere ; 4 SP - 141 EP - 152 PB - Campisano editore CY - Rom ER - TY - JOUR A1 - Wilke, Thomas T1 - Newly found plans for the chapel of the Holy Shroud JF - Studi Piemontesi Y1 - 2017 SN - 0392-7261 VL - XLVI IS - 1 SP - 75 EP - 85 ER - TY - JOUR A1 - Wild, Dominik A1 - Schrezenmeier, Johannes A1 - Czupalla, Markus A1 - Förstner, Markus T1 - Thermal Characterization of additive manufactured Integral Structures for Phase Change Applications JF - 2020 International Conference on Environmental Systems N2 - “Infused Thermal Solutions” (ITS) introduces a method for passive thermal control to stabilize structural components thermally without active heating and cooling systems, by using phase change material (PCM) in combination with lattice – both embedded into an additive manufactured integral structure. The technology is currently under development. This paper presents the results of the thermal property measurements performed on additive manufactured ITS breadboards. Within the breadboard campaigns key characteristics of the additive manufactured specimens were derived: Mechanical parameters: specimen impermeability, minimum wall thickness, lattice structure, subsequent heat treatment. Thermal properties: thermo-optical surface properties of the additive manufactured raw material, thermal conductivity and specific heat capacity measurements. As a conclusion the paper introduces an overview of potential ITS hardware applications, expected to increase the thermal performance. Y1 - 2020 PB - Texas Tech University ER - TY - JOUR A1 - Wiesen, Sebastian A1 - Tippkötter, Nils A1 - Muffler, Kai A1 - Suck, Kirstin A1 - Sohling, Ulrich A1 - Ruf, Friedrich A1 - Ulber, Roland T1 - Adsorption of fatty acids to layered double hydroxides in aqueous systems JF - Adsorption N2 - Due to their anion exchange characteristics, layered double hydroxides (LDHs) are suitable for the detoxification of aqueous, fatty acid containing fermentation substrates. The aim of this study is to examine the adsorption mechanism, using crude glycerol from plant oil esterification as a model system. Changes in the intercalation structure in relation to the amount of fatty acids adsorbed are monitored by X-ray diffraction and infra-red spectroscopy. Additionally, calcination of LDH is investigated in order to increase the binding capacity for fatty acids. Our data propose that, at ambient temperature, fatty acids can be bound to the hydrotalcite by adsorption or in addition by intercalation, depending on fatty acid concentration. The adsorption of fatty acids from crude glycerol shows a BET-like behavior. Above a fatty acid concentration of 3.5 g L−1, intercalation of fatty acids can be shown by the appearance of an increased interlayer spacing. This observation suggests a two phase adsorption process. Calcination of LDHs allows increasing the binding capacity for fatty acids by more than six times, mainly by reduction of structural CO32−. Y1 - 2015 VL - 21 IS - 6-7 SP - 459 EP - 466 PB - Springer CY - Berlin ER - TY - CHAP A1 - Wiesen, Patrick A1 - Engemann, Heiko A1 - Limpert, Nicolas A1 - Kallweit, Stephan T1 - Learning by Doing - Mobile Robotics in the FH Aachen ROS Summer School T2 - European Robotics Forum 2018, TRROS18 Workshop Y1 - 2018 SP - 47 EP - 58 ER - TY - CHAP A1 - Wiegner, Jonas A1 - Volker, Hanno A1 - Mainz, Fabian A1 - Backes, Andreas A1 - Löken, Michael A1 - Hüning, Felix T1 - Wiegand-effect-powered wireless IoT sensor node T2 - Sensoren und Messsysteme 2022 N2 - In this article we describe an Internet-of-Things sensing device with a wireless interface which is powered by the oftenoverlooked harvesting method of the Wiegand effect. The sensor can determine position, temperature or other resistively measurable quantities and can transmit the data via an ultra-low power ultra-wideband (UWB) data transmitter. With this approach we can energy-self-sufficiently acquire, process, and wirelessly transmit data in a pulsed operation. A proof-of-concept system was built up to prove the feasibility of the approach. The energy consumption of the system is analyzed and traced back in detail to the individual components, compared to the generated energy and processed to identify further optimization options. Based on the proof-of-concept, an application demonstrator was developed. Finally, we point out possible use cases. Y1 - 2022 SN - 978-3-8007-5835-7 SP - 255 EP - 260 PB - VDE Verlag GmbH CY - Berlin ER - TY - GEN A1 - Wiegner, J. A1 - Volker, H. A1 - Mainz, F. A1 - Backes, A. A1 - Löken, M. A1 - Hüning, Felix T1 - Wiegand-Effect-Powered Wireless IT Sensor Node N2 - With the growing interest in small distributed sensors for the “Internet of Things”, more attention is being paid to energy harvesting techologies. Reducing or eliminating the need for external power sources or batteries make devices more self-sufficient, more reliable, and reduces maintenance requirements. The Wiegand effect is a proven technology for harvesting small amounts of electrical power from mechanical motion. Y1 - 2022 N1 - PSMA International Energy Harvesting Workshop ~ 5-7 April 2022, Raleigh, NC, USA ER - TY - JOUR A1 - Wiegand, Sandra A1 - Voigt, Birgit A1 - Albrecht, Dirk A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Hecker, Michael A1 - Daniel, Rolf A1 - Liesegang, Heiko T1 - Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production JF - Microbial Cell Factories Y1 - 2013 U6 - http://dx.doi.org/10.1186/1475-2859-12-120 SN - 1475-2859 VL - 12 SP - 120 PB - Biomed Central CY - London ER - TY - JOUR A1 - Wiegand, Sandra A1 - Dietrich, Sascha A1 - Hertel, Robert A1 - Bongaerts, Johannes A1 - Evers, Stefan A1 - Volland, Sonja A1 - Daniel, Rolf A1 - Liesegang, Heiko T1 - RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation JF - BMC genomics Y1 - 2013 SN - 1471-2164 VL - Vol. 14 SP - 667 PB - BioMed Central CY - London ER - TY - JOUR A1 - Whitehead, Mark A1 - Öhlschläger, Peter A1 - Almajhdi, Fahad N. A1 - Alloza, Leonor A1 - Marzábal, Pablo A1 - Meyers, Ann E. A1 - Hitzeroth, Inga I. A1 - Rybicki, Edward P. T1 - Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice JF - BMC cancer Y1 - 2014 U6 - http://dx.doi.org/10.1186/1471-2407-14-367 SN - 1471-2407 IS - 14:367 SP - 1 EP - 15 PB - BioMed Central CY - London ER - TY - CHAP A1 - Wetter, Martin A1 - Kern, Alexander T1 - Number of lightning strikes to tall structures - comparison of calculations and measurements using a modern lightning monitoring system T2 - 2014 International Conference on Lightning Protection (ICLP), Shanghai, China Y1 - 2014 SP - 1 EP - 7 ER - TY - JOUR A1 - Wert, Stefan A1 - Iken, Heiko A1 - Schöning, Michael Josef A1 - Matysik, Frank-Michael T1 - Development of a temperature‐pulse enhanced electrochemical glucose biosensor and characterization of its stability via scanning electrochemical microscopy JF - Electroanalysis N2 - Glucose oxidase (GOx) is an enzyme frequently used in glucose biosensors. As increased temperatures can enhance the performance of electrochemical sensors, we investigated the impact of temperature pulses on GOx that was drop-coated on flattened Pt microwires. The wires were heated by an alternating current. The sensitivity towards glucose and the temperature stability of GOx was investigated by amperometry. An up to 22-fold increase of sensitivity was observed. Spatially resolved enzyme activity changes were investigated via scanning electrochemical microscopy. The application of short (<100 ms) heat pulses was associated with less thermal inactivation of the immobilized GOx than long-term heating. Y1 - 2021 U6 - http://dx.doi.org/10.1002/elan.202100089 SN - 1521-4109 IS - Early View PB - Wiley-VCH CY - Weinheim ER - TY - CHAP A1 - Werner, Frederik A1 - Yoshinobu, T. A1 - Miyamoto, K. A1 - Schöning, Michael Josef A1 - Wagner, Torsten T1 - Semiconductor-based sensors for imaging of chemical processes T2 - Sensoren und Messsysteme 2014 ; Beiträge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in Nürnberg. (ITG-Fachbericht ; 250) Y1 - 2014 SN - 978-3-8007-3622-5 SP - 1 EP - 5 PB - VDE-Verl. CY - Düsseldorf ER - TY - JOUR A1 - Werner, Frederik A1 - Wagner, Torsten A1 - Yoshinobu, Tatsuo A1 - Keusgen, Michael A1 - Schöning, Michael Josef T1 - Frequency behaviour of light-addressable potentiometric sensors JF - Physica Status Solidi (A) N2 - Light-addressable potentiometric sensors (LAPS) are semiconductor-based potentiometric sensors, with the advantage to detect the concentration of a chemical species in a liquid solution above the sensor surface in a spatially resolved manner. The addressing is achieved by a modulated and focused light source illuminating the semiconductor and generating a concentration-depending photocurrent. This work introduces a LAPS set-up that is able to monitor the electrical impedance in addition to the photocurrent. The impedance spectra of a LAPS structure, with and without illumination, as well as the frequency behaviour of the LAPS measurement are investigated. The measurements are supported by electrical equivalent circuits to explain the impedance and the LAPS-frequency behaviour. The work investigates the influence of different parameters on the frequency behaviour of the LAPS. Furthermore, the phase shift of the photocurrent, the influence of the surface potential as well as the changes of the sensor impedance will be discussed. Y1 - 2013 U6 - http://dx.doi.org/10.1002/pssa.201200929 SN - 1521-396X ; 0031-8965 VL - 210 IS - 5 SP - 884 EP - 891 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Werner, Frederik A1 - Wagner, Torsten A1 - Miyamoto, Ko-ichiro A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef T1 - High speed and high resolution chemical imaging based on a new type of OLED-LAPS set-up JF - Sensors and Actuators B: Chemical N2 - Light-addressable potentiometric sensors (LAPS) are field-effect-based sensors. A modulated light source is used to define the particular measurement spot to perform spatially resolved measurements of chemical species and to generate chemical images. In this work, an organic-LED (OLED) display has been chosen as a light source. This allows high measurement resolution and miniaturisation of the system. A new developed driving method for the OLED display optimised for LAPS-based measurements is demonstrated. The new method enables to define modulation frequencies between 1 kHz and 16 kHz and hence, reduces the measurement time of a chemical image by a factor of 40 compared to the traditional addressing of an OLED display. Y1 - 2012 U6 - http://dx.doi.org/10.1016/j.snb.2011.12.102 SN - 0925-4005 N1 - Part of special issue "Selected Papers presented at Eurosensors XXV" VL - 175 SP - 118 EP - 122 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Werner, Frederik A1 - Wagner, Torsten A1 - Miyamoto, Ko-ichiro A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef T1 - High speed and high resolution chemical imaging based on a new type of OLED-LAPS set-up JF - Procedia Engineering. 25 (2011) Y1 - 2011 SN - 1877-7058 N1 - EurosensorsXXV ; Proc. Eurosensors XXV, September 4-7, 2011, Athens, Greece SP - 346 EP - 349 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Werner, Frederik A1 - Takenaga, Shoko A1 - Taki, Hidenori A1 - Sawada, Kazuaki A1 - Schöning, Michael Josef T1 - Comparison of label-free ACh-imaging sensors based on CCD and LAPS JF - Sensors and Actuators B: Chemical (2012) N2 - Semiconductor-based chemical imaging sensors, like the light-addressable potentiometric sensor (LAPS) or the pH-imaging sensor based on a charge-coupled device (CCD), are becoming a powerful tool for label-free imaging of biological phenomena. We have proposed a polyion-based enzymatic membrane to develop an acetylcholine (ACh) imaging sensor for neural cell-activity observations. In this study, a CCD-type ACh-imaging sensor and a LAPS-type ACh-imaging sensor were fabricated and the prospect of both sensors was clarified by making a comparison of their basic characteristics. Y1 - 2013 SN - 0925-4005 VL - 177 SP - 745 EP - 752 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Werner, Frederik A1 - Schusser, Sebastian A1 - Spalthahn, Heiko A1 - Wagner, Torsten A1 - Yoshinobu, Tatsuo A1 - Schöning, Michael Josef T1 - Field-programmable gate array based controller for multi spot light-addressable potentiometric sensors with integrated signal correction mode JF - Electrochimica Acta N2 - A light-addressable potentiometric sensor (LAPS) can measure the concentration of one or several analytes at the sensor surface simultaneously in a spatially resolved manner. A modulated light pointer stimulates the semiconductor structure at the area of interest and a responding photocurrent can be read out. By simultaneous stimulation of several areas with light pointers of different modulation frequencies, the read out can be performed at the same time. With the new proposed controller electronic based on a field-programmable gate array (FPGA), it is possible to control the modulation frequencies, phase shifts, and light brightness of multiple light pointers independently and simultaneously. Thus, it is possible to investigate the frequency response of the sensor, and to examine the analyte concentration by the determination of the surface potential with the help of current/voltage curves and phase/voltage curves. Additionally, the ability to individually change the light intensities of each light pointer is used to perform signal correction. Y1 - 2011 U6 - http://dx.doi.org/10.1016/j.electacta.2011.03.012 SN - 0013-4686 VL - 56 IS - 26 SP - 9656 EP - 9660 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Werner, Frederik A1 - Miyamoto, Ko-ichiro A1 - Wagner, Torsten A1 - Schöning, Michael Josef A1 - Yoshinobu, Tatsuo T1 - Lateral resolution enhancement of pulse-driven light-addressable potentiometric sensor JF - Sensor and Actuators B: Chemical N2 - To study chemical and biological processes, spatially resolved determination of the concentrations of one or more analyte species is of distinct interest. With a light-addressable potentiometric sensor (LAPS), chemical images can be created, which visualize the concentration distribution above the sensor plate. One important challenge is to achieve a good lateral resolution in order to detect events that take place in a small and limited region. LAPS utilizes a focused light spot to address the measurement region. By moving this light spot along the semiconductor sensor plate, the concentration distribution can be observed. In this study, we show that utilizing a pulse as light excitation instead of a traditionally used continuously modulated light excitation, the lateral resolution can be improved by a factor of 6 or more. KW - Chemical images KW - LAPS KW - Light-addressable potentiometric sensor Y1 - 2017 U6 - http://dx.doi.org/10.1016/j.snb.2017.02.057 SN - 0925-4005 VL - 248 SP - 961 EP - 965 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Werner, Frederik A1 - Krumbe, Christoph A1 - Schumacher, Katharina A1 - Groebel, Simone A1 - Spelthahn, Heiko A1 - Stellberg, Michael A1 - Wagner, Torsten A1 - Yoshinobu, Tatsuo A1 - Selmer, Thorsten A1 - Keusgen, Michael A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor JF - Physica status solidi (a) : applications and material science. 208 (2011), H. 6 Y1 - 2011 SN - 1862-6319 SP - 1340 EP - 1344 PB - Wiley CY - Weinheim ER - TY - JOUR A1 - Werner, Frederik A1 - Groebel, Simone A1 - Krumbe, Christoph A1 - Wagner, Torsten A1 - Selmer, Thorsten A1 - Yoshinobu, Tatsuo A1 - Baumann, Marcus A1 - Schöning, Michael Josef T1 - Nutrient concentration-sensitive microorganism-based biosensor JF - Physica Status Solidi (a) Y1 - 2012 U6 - http://dx.doi.org/10.1002/pssa.201100801 SN - 1862-6319 VL - 209 IS - 5 SP - 900 EP - 904 PB - Wiley-VCH CY - Weinheim ER - TY - THES A1 - Werner, Frederik T1 - Development of light-addressable potentiometric sensor systems and their applications in biotechnological environments Y1 - 2014 N1 - Marburg, Univ., Diss., 2014 ER - TY - JOUR A1 - Werkhausen, Amelie A1 - Willwacher, Steffen A1 - Albracht, Kirsten T1 - Medial gastrocnemius muscle fascicles shorten throughout stance during sprint acceleration JF - Scandinavian Journal of Medicine & Science in Sports N2 - The compliant nature of distal limb muscle-tendon units is traditionally considered suboptimal in explosive movements when positive joint work is required. However, during accelerative running, ankle joint net mechanical work is positive. Therefore, this study aims to investigate how plantar flexor muscle-tendon behavior is modulated during fast accelerations. Eleven female sprinters performed maximum sprint accelerations from starting blocks, while gastrocnemius muscle fascicle lengths were estimated using ultrasonography. We combined motion analysis and ground reaction force measurements to assess lower limb joint kinematics and kinetics, and to estimate gastrocnemius muscle-tendon unit length during the first two acceleration steps. Outcome variables were resampled to the stance phase and averaged across three to five trials. Relevant scalars were extracted and analyzed using one-sample and two-sample t-tests, and vector trajectories were compared using statistical parametric mapping. We found that an uncoupling of muscle fascicle behavior from muscle-tendon unit behavior is effectively used to produce net positive mechanical work at the joint during maximum sprint acceleration. Muscle fascicles shortened throughout the first and second steps, while shortening occurred earlier during the first step, where negative joint work was lower compared with the second step. Elastic strain energy may be stored during dorsiflexion after touchdown since fascicles did not lengthen at the same time to dissipate energy. Thus, net positive work generation is accommodated by the reuse of elastic strain energy along with positive gastrocnemius fascicle work. Our results show a mechanism of how muscles with high in-series compliance can contribute to net positive joint work. KW - locomotion KW - muscle mechanics KW - running KW - sprint start KW - ultrasonography Y1 - 2021 U6 - http://dx.doi.org/10.1111/sms.13956 SN - 0905-7188 (Druckausgabe) SN - 1600-0838 (Onlineausgabe) VL - 31 IS - 7 SP - 1471 EP - 1480 PB - Wiley-Blackwell CY - Oxford ER - TY - JOUR A1 - Werkhausen, Amelie A1 - Cronin, Neil J. A1 - Albracht, Kirsten A1 - Paulsen, Gøran A1 - Larsen, Askild V. A1 - Bojsen-Møller, Jens A1 - Seynnes, Olivier R. T1 - Training-induced increase in Achilles tendon stiffness affects tendon strain pattern during running JF - PeerJ N2 - Background During the stance phase of running, the elasticity of the Achilles tendon enables the utilisation of elastic energy and allows beneficial contractile conditions for the triceps surae muscles. However, the effect of changes in tendon mechanical properties induced by chronic loading is still poorly understood. We tested the hypothesis that a training-induced increase in Achilles tendon stiffness would result in reduced tendon strain during the stance phase of running, which would reduce fascicle strains in the triceps surae muscles, particularly in the mono-articular soleus. Methods Eleven subjects were assigned to a training group performing isometric singleleg plantarflexion contractions three times per week for ten weeks, and another ten subjects formed a control group. Before and after the training period, Achilles tendon stiffness was estimated, and muscle-tendon mechanics were assessed during running at preferred speed using ultrasonography, kinematics and kinetics. Results Achilles tendon stiffness increased by 18% (P <0:01) in the training group, but the associated reduction in strain seen during isometric contractions was not statistically significant. Tendon elongation during the stance phase of running was similar after training, but tendon recoil was reduced by 30% (P <0:01), while estimated tendon force remained unchanged. Neither gastrocnemius medialis nor soleus fascicle shortening during stance was affected by training. Discussion These results show that a training-induced increase in Achilles tendon stiffness altered tendon behaviour during running. Despite training-induced changes in tendon mechanical properties and recoil behaviour, the data suggest that fascicle shortening patterns were preserved for the running speed that we examined. The asymmetrical changes in tendon strain patterns supports the notion that simple inseries models do not fully explain the mechanical output of the muscle-tendon unit during a complex task like running. KW - Achilles tendon KW - Stiffness KW - Running KW - Tendon properties KW - Architectural gear ratio Y1 - 2019 U6 - http://dx.doi.org/10.7717/peerj.6764 SN - 21678359 PB - Peer CY - London ER - TY - JOUR A1 - Werkhausen, Amelie A1 - Cronin, Neil J. A1 - Albracht, Kirsten A1 - Bojsen-Møller, Jens A1 - Seynnes, Olivier R. T1 - Distinct muscle-tendon interaction during running at different speeds and in different loading conditions JF - Journal of Applied Physiology Y1 - 2019 U6 - http://dx.doi.org/10.1152/japplphysiol.00710.2018 SN - 1522-1601 VL - 127 IS - 1 SP - 246 EP - 253 ER - TY - JOUR A1 - Werkhausen, Amelie A1 - Albracht, Kirsten A1 - Cronin, Neil J. A1 - Meier, Rahel A1 - Mojsen-Moeller, Jens A1 - Seynnes, Olivier R. T1 - Modulation of muscle-tendon interaction in the human triceps surae during an energy dissipation task JF - Journal of Experimental Biology Y1 - 2017 U6 - http://dx.doi.org/10.1242/jeb.164111 SN - 0022-0949 VL - 220 IS - 22 SP - 4141 EP - 4149 ER - TY - JOUR A1 - Werkhausen, Amelie A1 - Albracht, Kirsten A1 - Cronin, Neil J A1 - Paulsen, Gøran A1 - Bojsen-Møller, Jens A1 - Seynnes, Olivier R T1 - Effect of training-induced changes in achilles tendon stiffness on muscle-tendon behavior during landing JF - Frontiers in physiology N2 - During rapid deceleration of the body, tendons buffer part of the elongation of the muscle-tendon unit (MTU), enabling safe energy dissipation via eccentric muscle contraction. Yet, the influence of changes in tendon stiffness within the physiological range upon these lengthening contractions is unknown. This study aimed to examine the effect of training-induced stiffening of the Achilles tendon on triceps surae muscle-tendon behavior during a landing task. Twenty-one male subjects were assigned to either a 10-week resistance-training program consisting of single-leg isometric plantarflexion (n = 11) or to a non-training control group (n = 10). Before and after the training period, plantarflexion force, peak Achilles tendon strain and stiffness were measured during isometric contractions, using a combination of dynamometry, ultrasound and kinematics data. Additionally, testing included a step-landing task, during which joint mechanics and lengths of gastrocnemius and soleus fascicles, Achilles tendon, and MTU were determined using synchronized ultrasound, kinematics and kinetics data collection. After training, plantarflexion strength and Achilles tendon stiffness increased (15 and 18%, respectively), and tendon strain during landing remained similar. Likewise, lengthening and negative work produced by the gastrocnemius MTU did not change detectably. However, in the training group, gastrocnemius fascicle length was offset (8%) to a longer length at touch down and, surprisingly, fascicle lengthening and velocity were reduced by 27 and 21%, respectively. These changes were not observed for soleus fascicles when accounting for variation in task execution between tests. These results indicate that a training-induced increase in tendon stiffness does not noticeably affect the buffering action of the tendon when the MTU is rapidly stretched. Reductions in gastrocnemius fascicle lengthening and lengthening velocity during landing occurred independently from tendon strain. Future studies are required to provide insight into the mechanisms underpinning these observations and their influence on energy dissipation. KW - achilles tendon KW - energy absorption KW - energy dissipation KW - mechanical buffer KW - stiffness Y1 - 2018 U6 - http://dx.doi.org/10.3389/fphys.2018.00794 SN - 1664-042X IS - 9 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Werfel, Stanislas A1 - Günthner, Roman A1 - Hapfelmeier, Alexander A1 - Hanssen, Henner A1 - Kotliar, Konstantin A1 - Heemann, Uwe A1 - Schmaderer, Christoph ED - Guzik, Tomasz J. T1 - Identification of cardiovascular high-risk groups from dynamic retinal vessel signals using untargeted machine learning JF - Cardiovascular Research N2 - Dynamic retinal vessel analysis (DVA) provides a non-invasive way to assess microvascular function in patients and potentially to improve predictions of individual cardiovascular (CV) risk. The aim of our study was to use untargeted machine learning on DVA in order to improve CV mortality prediction and identify corresponding response alterations. KW - Machine learning KW - Retinal vessels KW - Microcirculation KW - Haemodialysis KW - Myocardial infarction and cardiac death Y1 - 2022 U6 - http://dx.doi.org/10.1093/cvr/cvab040 SN - 0008-6363 VL - 118 IS - 2 SP - 612 EP - 621 PB - Oxford University Press CY - Oxford ER - TY - CHAP A1 - Wendorff, Marion A1 - Eggert, Thorsten A1 - Pohl, Martina A1 - Dresen, Carola A1 - Müller, Michael A1 - Jaeger, Karl-Erich A1 - Sprenger, Georg A. A1 - Schürmann, Melanie A1 - Schürmann, Martin A1 - Johnen, Sandra A1 - Sprenger, Gerda A1 - Sahm, Hermann A1 - Inoue, Tomoyuki A1 - Schörken, Ulrich A1 - Breittaupt, Holger A1 - Frölich, Bettina A1 - Heim, Petra A1 - Iding, Hans A1 - Juchem, Bettina A1 - Siegert, Petra A1 - Kula, Maria-Regina A1 - Weckbecker, Andrea A1 - Hummel, Werner A1 - Fessner, Wolf-Dieter A1 - Elling, Lothar A1 - Wolberg, Michael A1 - Bode, Silke A1 - Feldmann, Ralf A1 - Geilenkirchen, Petra A1 - Schubert, Thomas A1 - Walter, Lydia A1 - Dünnwald, Thomas A1 - Demir, Ayhan S. A1 - Kolter-Jung, Doris A1 - Nitsche, Adam A1 - Dünkelmann, Pascal A1 - Cosp, Annabel A1 - Lingen, Bettina T1 - Catalytic asymmetric synthesis : section 2.2 T2 - Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ... Y1 - 2007 SN - 978-3-527-31473-7 SP - 298 EP - 413 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Wendlandt, Tim A1 - Koch, Claudia A1 - Britz, Beate A1 - Liedek, Anke A1 - Schmidt, Nora A1 - Werner, Stefan A1 - Gleba, Yuri A1 - Vahidpour, Farnoosh A1 - Welden, Melanie A1 - Poghossian, Arshak A1 - Schöning, Michael Josef T1 - Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System JF - Viruses N2 - Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes. KW - biosensor KW - horseradish peroxidase (HRP) KW - glucose oxidase (GOx) KW - enzyme cascade KW - turnip vein clearing virus (TVCV) KW - tobacco mosaic virus (TMV) Y1 - 2023 U6 - http://dx.doi.org/doi.org/10.3390/v15091951 SN - 1999-4915 N1 - This article belongs to the Special Issue "Tobamoviruses 2023" VL - 9 IS - 15 PB - MDPI CY - Basel ER - TY - JOUR A1 - Wemhöner, Carsten A1 - Hafner, Bernd A1 - Schwarzer, Klemens T1 - Simulation of solar thermal systems with CARNOT blockset in the environment MATLAB-Simulink Y1 - 2000 SP - 1 EP - 6 ER - TY - CHAP A1 - Wellmer, Georg A1 - Chen, B.-H. A1 - Braun, Carsten A1 - Ballmann, Josef T1 - Numerical prediction of aeroelastic effects on twin-sting-rig mounted models for rear fuselage and empennage flow investigation in transonic windtunnel T2 - Proceedings / IFASD 2007, CEAS/AIAA/KTH International Forum on Aeroelasticity and Structural Dynamics, June 18 - 21, 2007, Stockholm, Sweden Y1 - 2007 PB - KTH CY - Stockholm ER - TY - JOUR A1 - Welden, Rene A1 - Schöning, Michael Josef A1 - Wagner, Patrick H. A1 - Wagner, Torsten T1 - Light-Addressable Electrodes for Dynamic and Flexible Addressing of Biological Systems and Electrochemical Reactions JF - Sensors N2 - In this review article, we are going to present an overview on possible applications of light-addressable electrodes (LAE) as actuator/manipulation devices besides classical electrode structures. For LAEs, the electrode material consists of a semiconductor. Illumination with a light source with the appropiate wavelength leads to the generation of electron-hole pairs which can be utilized for further photoelectrochemical reaction. Due to recent progress in light-projection technologies, highly dynamic and flexible illumination patterns can be generated, opening new possibilities for light-addressable electrodes. A short introduction on semiconductor–electrolyte interfaces with light stimulation is given together with electrode-design approaches. Towards applications, the stimulation of cells with different electrode materials and fabrication designs is explained, followed by analyte-manipulation strategies and spatially resolved photoelectrochemical deposition of different material types. Y1 - 2020 U6 - http://dx.doi.org/10.3390/s20061680 SN - 1424-8220 VL - 20 IS - 6 PB - MDPI CY - Basel ER - TY - JOUR A1 - Welden, Rene A1 - Scheja, Sabrina A1 - Schöning, Michael Josef A1 - Wagner, Patrick A1 - Wagner, Torsten T1 - Electrochemical Evaluation of Light‐Addressable Electrodes Based on TiO2 for the Integration in Lab‐on‐Chip Systems JF - physica status solidi a : applications and materials sciences N2 - In lab-on-chip systems, electrodes are important for the manipulation (e.g., cell stimulation, electrolysis) within such systems. An alternative to commonly used electrode structures can be a light-addressable electrode. Here, due to the photoelectric effect, the conducting area can be adjusted by modification of the illumination area which enables a flexible control of the electrode. In this work, titanium dioxide based light-addressable electrodes are fabricated by a sol–gel technique and a spin-coating process, to deposit a thin film on a fluorine-doped tin oxide glass. To characterize the fabricated electrodes, the thickness, and morphological structure are measured by a profilometer and a scanning electron microscope. For the electrochemical behavior, the dark current and the photocurrent are determined for various film thicknesses. For the spatial resolution behavior, the dependency of the photocurrent while changing the area of the illuminated area is studied. Furthermore, the addressing of single fluid compartments in a three-chamber system, which is added to the electrode, is demonstrated. Y1 - 2018 U6 - http://dx.doi.org/10.1002/pssa.201800150 SN - 1862-6319 VL - 215 IS - 15 SP - Article number 1800150 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Welden, Rene A1 - Jablonski, Melanie A1 - Wege, Christina A1 - Keusgen, Michael A1 - Wagner, Patrick Hermann A1 - Wagner, Torsten A1 - Schöning, Michael Josef T1 - Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase JF - Biosensors N2 - The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS. KW - microfluidics KW - enzyme kinetics KW - actuator-sensor system KW - light-addressable electrode KW - light-addressable potentiometric sensor Y1 - 2021 U6 - http://dx.doi.org/10.3390/bios11060171 SN - 2079-6374 N1 - This article belongs to the Special Issue "Selected Papers from the 1st International Electronic Conference on Biosensors (IECB 2020)" VL - 11 IS - 6 PB - MDPI CY - Basel ER -